Expressing Biologically Active Membrane Proteins in a Cell
... Fluorescence-based carazolol binding assay uses (S)-carazolol, a derivative of the potent β blocker carazolol with fluorescence properties (ex633nm/em650nm). This ligand can be used as a fluorescence tracker for β2AR binding activity. The detailed experimental setup is described in Mat ...
... Fluorescence-based carazolol binding assay uses (S)-carazolol, a derivative of the potent β blocker carazolol with fluorescence properties (ex633nm/em650nm). This ligand can be used as a fluorescence tracker for β2AR binding activity. The detailed experimental setup is described in Mat ...
Green Fluorescent Protein (GFP) Purification Kit
... Transformation—the pGLO System” (catalog number 166-0003-EDU). Students begin this activity with the bacteria they genetically transformed using the plasmid, pGLO. Transformed bacteria which produce the genetically engineered Green Fluorescent Protein (GFP) are removed from their agar plates and all ...
... Transformation—the pGLO System” (catalog number 166-0003-EDU). Students begin this activity with the bacteria they genetically transformed using the plasmid, pGLO. Transformed bacteria which produce the genetically engineered Green Fluorescent Protein (GFP) are removed from their agar plates and all ...
Títol del treball:
... billion). Current estimates indicate that we need to increase food production by 70% in the next 40 years. Recent reports form the FAO (Food and Agriculture Organization of the United Nations) expose that the number of people in the world who are chronically hungry crossed the one billion mark in 20 ...
... billion). Current estimates indicate that we need to increase food production by 70% in the next 40 years. Recent reports form the FAO (Food and Agriculture Organization of the United Nations) expose that the number of people in the world who are chronically hungry crossed the one billion mark in 20 ...
Multiple Sequence Motifs in the Rubisco Small
... targeting, we constructed mutants, E1aT1[TTT/ AAA], E1aT1[FPL/AAA], and E1aT1[K/A], that contained Ala substitution of the three Thrs (TTT), the three hydrophobic amino acids (FPL), and the single Lys (K), respectively (Fig. 2C). These mutants were fused to GFP and then we examined their ability to ...
... targeting, we constructed mutants, E1aT1[TTT/ AAA], E1aT1[FPL/AAA], and E1aT1[K/A], that contained Ala substitution of the three Thrs (TTT), the three hydrophobic amino acids (FPL), and the single Lys (K), respectively (Fig. 2C). These mutants were fused to GFP and then we examined their ability to ...
C. elegans daf-6 Encodes a Patched-Related Protein
... present early during channel development. Indeed, young daf-6(e1377) larvae did not frequently contain bent cilia or vacuolar structures. However, dye uptake was completely blocked at all stages, indicating that channel neurons always lacked access to the outside environment. Furthermore, animals at ...
... present early during channel development. Indeed, young daf-6(e1377) larvae did not frequently contain bent cilia or vacuolar structures. However, dye uptake was completely blocked at all stages, indicating that channel neurons always lacked access to the outside environment. Furthermore, animals at ...
DOC
... contributions to circuit rearrangements by presynaptic terminals have remained unclear. We used hippocampal slice cultures from mice expressing spectral variants of green fluorescent protein (GFP) that are targeted to the membrane and/or synaptic vesicles in neuronal subsets to image identified pres ...
... contributions to circuit rearrangements by presynaptic terminals have remained unclear. We used hippocampal slice cultures from mice expressing spectral variants of green fluorescent protein (GFP) that are targeted to the membrane and/or synaptic vesicles in neuronal subsets to image identified pres ...
A Class of Human Proteins that Deliver Functional
... in the current study possess positively charged amino acids widely dispersed throughout their sequence, and would not have been identified using these previously reported methods. Moreover, the NSHPs we have identified were able to mediate internalization of fused proteins with up to 40-fold higher ...
... in the current study possess positively charged amino acids widely dispersed throughout their sequence, and would not have been identified using these previously reported methods. Moreover, the NSHPs we have identified were able to mediate internalization of fused proteins with up to 40-fold higher ...
regulates cortical cell migration out of the
... independent clones, one novel clone (designated later as filip) showed restricted expression in the ventricular zone of the neocortex (Fig. 1a). In addition to the nervous system, filip mRNA was also observed in muscular tissues, including the cardiovascular system. Two full-length FILIP cDNAs were ...
... independent clones, one novel clone (designated later as filip) showed restricted expression in the ventricular zone of the neocortex (Fig. 1a). In addition to the nervous system, filip mRNA was also observed in muscular tissues, including the cardiovascular system. Two full-length FILIP cDNAs were ...
C.Prinz, J.O. Tegenfeldt, R.H. Austin, E.C. Cox, J.C. Sturm, "Bacterial chromosome extraction and isolation," Lab Chip, 2, pp. 207-212 (2002).
... where q is the effective charge and a(w) is the polarizability of the object at the angular frequency w. The first term in the force expression corresponds to the electrophoretic force, which tends to move the object in the electric field direction. The second term corresponds to the dielectric forc ...
... where q is the effective charge and a(w) is the polarizability of the object at the angular frequency w. The first term in the force expression corresponds to the electrophoretic force, which tends to move the object in the electric field direction. The second term corresponds to the dielectric forc ...
GroupMeeting(BioSketch)-CollinsMod-_2005-07
... pWG+pWCI transformants (without IPTG or UV) give no fluorescence. Digests of those transformants clearly indicates that pWG is present. pWG-only controls glow. ...
... pWG+pWCI transformants (without IPTG or UV) give no fluorescence. Digests of those transformants clearly indicates that pWG is present. pWG-only controls glow. ...
Dave - Jin Lab
... 1.Q.C. PCR – two rounds to shift flanking amino acid, thereby shifting ‘insert’ ...
... 1.Q.C. PCR – two rounds to shift flanking amino acid, thereby shifting ‘insert’ ...
The role of cell cycle–regulated expression in the
... budding yeast Saccharomyces cerevisiae, such polarization allows asymmetric growth to form a bud, which becomes the daughter cell. An important feature of cell polarization is the selection of an axis. In S. cerevisiae, this is manifested in the selection of bud sites, which occurs in two distinct p ...
... budding yeast Saccharomyces cerevisiae, such polarization allows asymmetric growth to form a bud, which becomes the daughter cell. An important feature of cell polarization is the selection of an axis. In S. cerevisiae, this is manifested in the selection of bud sites, which occurs in two distinct p ...
Protein Physics by Computer. Step by Step: Protein Visualization
... splitting and as a consequence for oxygen evolution on earth. By far most of the atmospheric oxygen originates from this source and is probably the only source to replenish oxygen on a large scale. The appearance of oxygen on a large scale did not only trigger evolution of more sophisticated life fo ...
... splitting and as a consequence for oxygen evolution on earth. By far most of the atmospheric oxygen originates from this source and is probably the only source to replenish oxygen on a large scale. The appearance of oxygen on a large scale did not only trigger evolution of more sophisticated life fo ...
8879.full - The Journal of Neuroscience
... in neuronal fate determination. For example, delivery of miR124 duplexes to HeLa cells induces a neuronal gene profile (Lim et al., 2005), and forced expression of miR-124 in progenitor cells causes neuronal differentiation (Visvanathan et al., 2007; Yu et al., 2008). Nevertheless, despite the obser ...
... in neuronal fate determination. For example, delivery of miR124 duplexes to HeLa cells induces a neuronal gene profile (Lim et al., 2005), and forced expression of miR-124 in progenitor cells causes neuronal differentiation (Visvanathan et al., 2007; Yu et al., 2008). Nevertheless, despite the obser ...
PilB localization determines the direction of twitching
... plates (Rippka et al., 1979) at 30°C under white light of 50 µmol photons m -2 s-1. For ...
... plates (Rippka et al., 1979) at 30°C under white light of 50 µmol photons m -2 s-1. For ...
to get the file - Oxford Brookes University
... hindered the systematic isolation of mutants defective in various membrane-transport processes. To facilitate systematic studies of gene function in membrane traf®c during plant development, we developed a visual screen and biochemical assay system for potential mutants with perturbed endomembrane d ...
... hindered the systematic isolation of mutants defective in various membrane-transport processes. To facilitate systematic studies of gene function in membrane traf®c during plant development, we developed a visual screen and biochemical assay system for potential mutants with perturbed endomembrane d ...
Supporting online material for Elowitz et al. report
... Supporting online material for Elowitz et al. report ...
... Supporting online material for Elowitz et al. report ...
Non-Targeted and Targeted Protein Movement
... Intercellular transit through plasmodesmata is an absolute required function for viral movement proteins. As plasmodesmata provide an impediment to viral local and systemic movement, viruses have evolved these proteins, capable of manipulating these channels, to facilitate entrance to neighboring ce ...
... Intercellular transit through plasmodesmata is an absolute required function for viral movement proteins. As plasmodesmata provide an impediment to viral local and systemic movement, viruses have evolved these proteins, capable of manipulating these channels, to facilitate entrance to neighboring ce ...
Y41G9a.1 and ciliary defects in osm
... with altered cilia structure has revealed that many of the proteins are homologs of IFT proteins identified in Chlamydomonas (Cole et al., 1998). The protein products of the worm osm-1 and osm-6 genes are homologs of IFT raft proteins identified in Chlamydomonas, while che-3 and osm-3 encode the dyn ...
... with altered cilia structure has revealed that many of the proteins are homologs of IFT proteins identified in Chlamydomonas (Cole et al., 1998). The protein products of the worm osm-1 and osm-6 genes are homologs of IFT raft proteins identified in Chlamydomonas, while che-3 and osm-3 encode the dyn ...
Zhang YA, Okada A, Lew CH, McConnell SK
... main, was deleted (GFP–⌬N97, –⌬N250, and –⌬N309: Figs. 4D– 4F) adopted a distribution identical to that of GFP alone, with protein visible in both the cytoplasm and the nucleus. These results are consistent with the hypothesis that the RKQRRER sequence within the homeodomain plays an important role ...
... main, was deleted (GFP–⌬N97, –⌬N250, and –⌬N309: Figs. 4D– 4F) adopted a distribution identical to that of GFP alone, with protein visible in both the cytoplasm and the nucleus. These results are consistent with the hypothesis that the RKQRRER sequence within the homeodomain plays an important role ...
Sorting of plant vacuolar proteins is initiated in the ER
... However, although individual expression of SNX1 or SNX2a mutants altered transport of the VSR, vacuolar delivery of soluble cargo was only marginally perturbed (Niemes et al., 2009). This raised the question as to whether retromer function is completely inhibited under these conditions, as it has be ...
... However, although individual expression of SNX1 or SNX2a mutants altered transport of the VSR, vacuolar delivery of soluble cargo was only marginally perturbed (Niemes et al., 2009). This raised the question as to whether retromer function is completely inhibited under these conditions, as it has be ...
Migration Cues Induce Chromatin Alterations
... cells (Figure 3A–C), indicating typical H1 nuclear distribution (15,16). In addition, the GFP-fused protein was released from the nuclei by stepwise salt extraction similar to endogenous H1 (Figure 3D), indicating compatible chromatin-binding properties. Next, the dynamics of migrationinduced change ...
... cells (Figure 3A–C), indicating typical H1 nuclear distribution (15,16). In addition, the GFP-fused protein was released from the nuclei by stepwise salt extraction similar to endogenous H1 (Figure 3D), indicating compatible chromatin-binding properties. Next, the dynamics of migrationinduced change ...
Photoactivation mechanism of PAmCherry based on crystal
... cellular and even macromolecular structure that were not previously discernible with less than 40 nm resolution (1). There is significant demand for a broader and more diverse range of photoactivatable fluorescent probes (2), in particular irreversibly photoactivatable fluorescent proteins (PAFPs). ...
... cellular and even macromolecular structure that were not previously discernible with less than 40 nm resolution (1). There is significant demand for a broader and more diverse range of photoactivatable fluorescent probes (2), in particular irreversibly photoactivatable fluorescent proteins (PAFPs). ...
The Plastidial 2-C-Methyl-D-Erythritol 4-Phosphate
... solely responsible for the biosynthesis of the isoprenyl diphosphates used by plant cells for protein isoprenylation. Indeed, incubation of BY-2 cells in the presence of [2-14C]1-deoxy-Dxylulose (DX), the dephosphorylated form of the first product of the MEP pathway, resulted in significant labeling ...
... solely responsible for the biosynthesis of the isoprenyl diphosphates used by plant cells for protein isoprenylation. Indeed, incubation of BY-2 cells in the presence of [2-14C]1-deoxy-Dxylulose (DX), the dephosphorylated form of the first product of the MEP pathway, resulted in significant labeling ...
pdf - Biotium
... particularly membrane proteins such as ion channels. The reagents selectively and rapidly react with thiols (sulfhydryls) to form a disulfide bond and as a result are highly efficient labeling agents for cysteine residues in proteins. The so-called SCAM method (substituted-cysteine accessibility met ...
... particularly membrane proteins such as ion channels. The reagents selectively and rapidly react with thiols (sulfhydryls) to form a disulfide bond and as a result are highly efficient labeling agents for cysteine residues in proteins. The so-called SCAM method (substituted-cysteine accessibility met ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.