technicolour transgenics: imaging tools for functional
... GFP, and the first strains of transgenic mice that expressed readily detectable GFP variants were reported approximately five years ago22,23. Fluorescent proteins were also mutated to give reporters with different spectral properties, so in vivo multicolour labelling experiments are now possible if ...
... GFP, and the first strains of transgenic mice that expressed readily detectable GFP variants were reported approximately five years ago22,23. Fluorescent proteins were also mutated to give reporters with different spectral properties, so in vivo multicolour labelling experiments are now possible if ...
GFP is the way to glow: bioimaging of the plant endomembrane
... and microfilaments labelled with fluorescent proteins. 2.3. Golgi imaging in vivo Studies on the dynamics of the plant Golgi are relatively young in comparison with their mammalian counterparts, due to the absence of genuine plant Golgi enzymes to be fused to GFP and used as in vivo markers. The fir ...
... and microfilaments labelled with fluorescent proteins. 2.3. Golgi imaging in vivo Studies on the dynamics of the plant Golgi are relatively young in comparison with their mammalian counterparts, due to the absence of genuine plant Golgi enzymes to be fused to GFP and used as in vivo markers. The fir ...
Disulphide-bond formation in protein folding catalysed by highly
... used to compensate for fluctuations in excitation and alterations photomultiplier in the field of view (Harbig et al., 1976) or for measuring changes in dye absorbance. A diagram of the experimental arrangement is shown in Fig. 1. Optical signals were recorded cence measurements. The cells were then ...
... used to compensate for fluctuations in excitation and alterations photomultiplier in the field of view (Harbig et al., 1976) or for measuring changes in dye absorbance. A diagram of the experimental arrangement is shown in Fig. 1. Optical signals were recorded cence measurements. The cells were then ...
Regulators of Lysosome Function and Dynamics in Caenorhabditis
... that cup-12 to cup-17 mutants had brighter GFP in coelomocytes, indicating a defect in lysosomal transport and/or function in these mutants (Figure 1, A and B). The brighter GFP in coelomocytes of cup-13 and cup-16 mutants is an underestimate because of the reduced uptake of GFP into coelomocytes fr ...
... that cup-12 to cup-17 mutants had brighter GFP in coelomocytes, indicating a defect in lysosomal transport and/or function in these mutants (Figure 1, A and B). The brighter GFP in coelomocytes of cup-13 and cup-16 mutants is an underestimate because of the reduced uptake of GFP into coelomocytes fr ...
Targeting to the T. gondii plastid
... composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis (Köhler et al., 1997). The inner two membranes are presumab ...
... composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis (Köhler et al., 1997). The inner two membranes are presumab ...
Good & Bad Behaviour: Expected & Unexpected Annulations of
... We have explored cyclic amidines as conformationally restricted amide bond replacements, and have prepared imidazolines (1)1 and TETRAHYDROPYRIMIDINES (2)2 as pseudodipeptides. ...
... We have explored cyclic amidines as conformationally restricted amide bond replacements, and have prepared imidazolines (1)1 and TETRAHYDROPYRIMIDINES (2)2 as pseudodipeptides. ...
Protein Labeling
... Effective design criteria for site-specific protein labeling in living systems : • Receptor moieties must be amenable to genetic encoding as fusions to the protein of interest. • The receptors must be relatively small so as to not perturb protein function — an ideal receptor would be a short peptid ...
... Effective design criteria for site-specific protein labeling in living systems : • Receptor moieties must be amenable to genetic encoding as fusions to the protein of interest. • The receptors must be relatively small so as to not perturb protein function — an ideal receptor would be a short peptid ...
Regulators of Lysosome Function and Dynamics in Caenorhabditis
... progeny for worms that had coelomocytes with brighter GFP, indicative of a delay in the lysosomal degradation of endocytosed GFP in coelomocytes. We identified 20 independent homozygous mutants that gave viable progeny (Table 1). All of the mutations were recessive since crossing them to the parent ...
... progeny for worms that had coelomocytes with brighter GFP, indicative of a delay in the lysosomal degradation of endocytosed GFP in coelomocytes. We identified 20 independent homozygous mutants that gave viable progeny (Table 1). All of the mutations were recessive since crossing them to the parent ...
Gene Silencing without DNA: RNA-Mediated Cross
... were inoculated with PVX vectors. These vectors, shown in Figure 2B, were PVX–GUS, which carries the b-glucuronidase (GUS) reporter gene (Chapman et al., 1992), and PVX– GUSGF. The PVX–GUSGF vector is a derivative of PVX–GUS, with 363 nucleotides of 5 9 GFP sequence inserted downstream of the GUS ge ...
... were inoculated with PVX vectors. These vectors, shown in Figure 2B, were PVX–GUS, which carries the b-glucuronidase (GUS) reporter gene (Chapman et al., 1992), and PVX– GUSGF. The PVX–GUSGF vector is a derivative of PVX–GUS, with 363 nucleotides of 5 9 GFP sequence inserted downstream of the GUS ge ...
Advanced techniques
... Fluorescence Correlation Spectroscopy Small volume only a few molecules random fluctuations • Study the noise • Conclude about random processes at different time scales Small excitation volume Random molecular processes: • Diffuse in and out • Adopt different states • Bind or react ...
... Fluorescence Correlation Spectroscopy Small volume only a few molecules random fluctuations • Study the noise • Conclude about random processes at different time scales Small excitation volume Random molecular processes: • Diffuse in and out • Adopt different states • Bind or react ...
Vacuolar system distribution in Arabidopsis
... The leaf epidermal cells accumulating the lytic vacuolar Aleu-GFP showed a faint ¯uorescence apparently localized in the central vacuole (Fig. 3c). Aleu-GFP also was visible as a faint diffuse ¯uorescence in meristemoids but, in fully differentiated stomata, ¯uorescence decreased to the limit of det ...
... The leaf epidermal cells accumulating the lytic vacuolar Aleu-GFP showed a faint ¯uorescence apparently localized in the central vacuole (Fig. 3c). Aleu-GFP also was visible as a faint diffuse ¯uorescence in meristemoids but, in fully differentiated stomata, ¯uorescence decreased to the limit of det ...
Molecular sieving properties of the cytoplasm of Escherichia coli and
... rug.nl; Tel. (+31) 50 3634190; Fax (+31) 50 3634165. ...
... rug.nl; Tel. (+31) 50 3634190; Fax (+31) 50 3634165. ...
Presentation - people.vcu.edu
... order to gain insight on which domains of the ptk7 protein are required in this pathway, Hayes et al tested the deletion and substitution of PTK7 domains ability to ...
... order to gain insight on which domains of the ptk7 protein are required in this pathway, Hayes et al tested the deletion and substitution of PTK7 domains ability to ...
Plant RanGAPs are localized at the nuclear envelope in interphase
... These Arabidopsis cDNAs were closely related but not identical, and showed about 62 and 59% similarity to the MsRanGAP sequence (GenBank accession numbers AF214559and AF214560, respectively). To examine whether the two Arabidopsis RanGAP genes can provide the functions of the budding-yeast rna1 gene ...
... These Arabidopsis cDNAs were closely related but not identical, and showed about 62 and 59% similarity to the MsRanGAP sequence (GenBank accession numbers AF214559and AF214560, respectively). To examine whether the two Arabidopsis RanGAP genes can provide the functions of the budding-yeast rna1 gene ...
投影片 1
... A donor chromophore in its excited state can transfer energy by a nonradiative, long-range dipole-dipole coupling mechanism to an acceptor chromophore in close proximity (typically <10nm). ...
... A donor chromophore in its excited state can transfer energy by a nonradiative, long-range dipole-dipole coupling mechanism to an acceptor chromophore in close proximity (typically <10nm). ...
Multiple Exocytotic Markers Accumulate at the Sites of Perifungal
... a role in fungal accommodation inside the host cells. From this perspective, the composition of the PPA, which has been partially elucidated, gives clear indications. Beside the nucleus, whose movements to and from the fungal contact site are associated with the initiation and full development of th ...
... a role in fungal accommodation inside the host cells. From this perspective, the composition of the PPA, which has been partially elucidated, gives clear indications. Beside the nucleus, whose movements to and from the fungal contact site are associated with the initiation and full development of th ...
Poster Presentation
... behavior of the proteins using an epifluorescence microscope. Over 200 transcription factors were monitored under various stress conditions to observe which ones display this bursting behavior. We hope to understand which transcription factors display bursting and under what ...
... behavior of the proteins using an epifluorescence microscope. Over 200 transcription factors were monitored under various stress conditions to observe which ones display this bursting behavior. We hope to understand which transcription factors display bursting and under what ...
Blm10 binds to preactivated proteasome core particles with open
... As the interaction between N-terminal residues of a3 and a4 imposes gate closure on CP, we also tested the open gate mutant a4DN. Consistent with our observations above, both open a-rings of the a4DN CP are capped either by Blm10 or by RP. Again, Blm10 is an abundant protein and only a few CPs are b ...
... As the interaction between N-terminal residues of a3 and a4 imposes gate closure on CP, we also tested the open gate mutant a4DN. Consistent with our observations above, both open a-rings of the a4DN CP are capped either by Blm10 or by RP. Again, Blm10 is an abundant protein and only a few CPs are b ...
Austin Texas Championship Poster
... Motivation and Background In recent years, the ability to expand the genetic code has been made possible by re-coding the amber stop codon, UAG, via the use of modified tRNA synthetase/tRNA pairs. The modified tRNA synthetase charges the modified tRNA with a non-canonical amino acid (ncAA), an amino ...
... Motivation and Background In recent years, the ability to expand the genetic code has been made possible by re-coding the amber stop codon, UAG, via the use of modified tRNA synthetase/tRNA pairs. The modified tRNA synthetase charges the modified tRNA with a non-canonical amino acid (ncAA), an amino ...
Identification of a Fluorescent Protein from Rhacostoma Atlantica
... (19). All three proteins have a tyrosine in position number 2 within the chromophore. So, in the denatured state, the chromophore absorbance peak of all three of these proteins becomes independent of the different protein environments in which they find themselves in the native state. Knowing that Rh ...
... (19). All three proteins have a tyrosine in position number 2 within the chromophore. So, in the denatured state, the chromophore absorbance peak of all three of these proteins becomes independent of the different protein environments in which they find themselves in the native state. Knowing that Rh ...
Vacuolar system distribution in Arabidopsis tissues
... The leaf epidermal cells accumulating the lytic vacuolar Aleu-GFP showed a faint ¯uorescence apparently localized in the central vacuole (Fig. 3c). Aleu-GFP also was visible as a faint diffuse ¯uorescence in meristemoids but, in fully differentiated stomata, ¯uorescence decreased to the limit of det ...
... The leaf epidermal cells accumulating the lytic vacuolar Aleu-GFP showed a faint ¯uorescence apparently localized in the central vacuole (Fig. 3c). Aleu-GFP also was visible as a faint diffuse ¯uorescence in meristemoids but, in fully differentiated stomata, ¯uorescence decreased to the limit of det ...
Diffusion of Green Fluorescent Protein in Three Cell
... strain MC4100, which was selected for arabinose resistance (MC4100AR) (2). The GFP gene was GFPmut3* (6). GFP constructs were expressed using the arabinose-inducible pBAD24 vector (10). The TatA-GFP construct is described in reference 19, and the TorA-GFP construct is described in reference 23. TorA ...
... strain MC4100, which was selected for arabinose resistance (MC4100AR) (2). The GFP gene was GFPmut3* (6). GFP constructs were expressed using the arabinose-inducible pBAD24 vector (10). The TatA-GFP construct is described in reference 19, and the TorA-GFP construct is described in reference 23. TorA ...
Expression of GFP-fusions in Arabidopsis companion cells reveals
... correlated precisely with the regions exhibiting apoplastic sucrose loading and lacking GFP-unloading from the phloem in AtSUC2-promoter::GFP plants (Wright et al., 2003). These data confirmed that the sites of AtSUC2 promoter-driven GFP expression represent the sites of active phloem loading into t ...
... correlated precisely with the regions exhibiting apoplastic sucrose loading and lacking GFP-unloading from the phloem in AtSUC2-promoter::GFP plants (Wright et al., 2003). These data confirmed that the sites of AtSUC2 promoter-driven GFP expression represent the sites of active phloem loading into t ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.