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... its physiological relevance. Activity-dependent changes in nuclear localization have also been noted in DVE-1’s mammalian homologs, SATB1 and SATB2 (Cai et al., 2003; Dobreva et al., 2003), but the relationship of those findings to ours remains unknown. Chromatin immunoprecipitation of DVE-1::GFP co ...
... its physiological relevance. Activity-dependent changes in nuclear localization have also been noted in DVE-1’s mammalian homologs, SATB1 and SATB2 (Cai et al., 2003; Dobreva et al., 2003), but the relationship of those findings to ours remains unknown. Chromatin immunoprecipitation of DVE-1::GFP co ...
Lats kinase is involved in the intestinal apical
... localizes to the subapical region of epithelial cells By searching the C. elegans genome database, we have identified a single gene that encodes a Lats kinase, wts-1. WTS-1 has features of the NDR kinase family, including long N-terminal sequences (Fig. 1A; see Fig. S1 in the supplementary material) ...
... localizes to the subapical region of epithelial cells By searching the C. elegans genome database, we have identified a single gene that encodes a Lats kinase, wts-1. WTS-1 has features of the NDR kinase family, including long N-terminal sequences (Fig. 1A; see Fig. S1 in the supplementary material) ...
Imaging of plant dynamin-related proteins and clathrin around the
... plant endocytosis, as has been shown to be the case for dynamin and clathrin in animals. Unlike animal cytokinesis, plant cytokinesis involves the transport of massive amounts of membrane into the division plane to construct a large membranous organelle called the cell plate, during which exo/endocy ...
... plant endocytosis, as has been shown to be the case for dynamin and clathrin in animals. Unlike animal cytokinesis, plant cytokinesis involves the transport of massive amounts of membrane into the division plane to construct a large membranous organelle called the cell plate, during which exo/endocy ...
Using intrinsically fluorescent proteins for plant cell
... coexpression studies. The most commonly used constitutive promoters include those from actin, ubiquitin and the cauliflower mosaic virus 35S promoter. However, these promoters often drive expression to high levels raising the possibility of overexpression and ectopic-expression-related artifacts. Re ...
... coexpression studies. The most commonly used constitutive promoters include those from actin, ubiquitin and the cauliflower mosaic virus 35S promoter. However, these promoters often drive expression to high levels raising the possibility of overexpression and ectopic-expression-related artifacts. Re ...
The role of Pex3p in early events of peroxisome biogenesis in
... endomembrane system in one specific case of peroxisome biogenesis. We showed that synthesis of the first 50 amino acids of Pex3p (Pex3p[1-50]) resulted in the formation of vesicles that arose from the nuclear envelope (Faber et al., 2002, chapter 4). These vesicles had the potential to develop into ...
... endomembrane system in one specific case of peroxisome biogenesis. We showed that synthesis of the first 50 amino acids of Pex3p (Pex3p[1-50]) resulted in the formation of vesicles that arose from the nuclear envelope (Faber et al., 2002, chapter 4). These vesicles had the potential to develop into ...
Bacterial Transformation with pGlo Overview
... • The araPBAD promoter is often used in expression vectors • The pGlo plasmid has been modified to incorporate some features of the arabinose operon. • Both the araPBAD promoter and the araC gene are present. • The genes which code for araA, araB and araD have been replaced by the gene which codes f ...
... • The araPBAD promoter is often used in expression vectors • The pGlo plasmid has been modified to incorporate some features of the arabinose operon. • Both the araPBAD promoter and the araC gene are present. • The genes which code for araA, araB and araD have been replaced by the gene which codes f ...
GFP-labelled Rubisco and aspartate aminotransferase are present
... the twentieth century, and there have been sporadic observations since then, but a clear understanding of their structure or function has never been achieved (KoÈhler et al., 1997; Gray et al., 2001). Recently, the use of green ¯uorescent protein (GFP) targeted to plastids has facilitated the study ...
... the twentieth century, and there have been sporadic observations since then, but a clear understanding of their structure or function has never been achieved (KoÈhler et al., 1997; Gray et al., 2001). Recently, the use of green ¯uorescent protein (GFP) targeted to plastids has facilitated the study ...
Enriched Motor Neuron Populations Derived From Bacterial Artificial
... Grin2A (N-methyl-D-asparate receptor subunit 2A), Ret (glial-derived neurotrophic factor receptor),14 and Slc5A7 (choline transporter).7 The majority of the 49 genes have not been implicated before in motor neuron biology. To validate the results of the transcriptome analysis, we performed immunoflu ...
... Grin2A (N-methyl-D-asparate receptor subunit 2A), Ret (glial-derived neurotrophic factor receptor),14 and Slc5A7 (choline transporter).7 The majority of the 49 genes have not been implicated before in motor neuron biology. To validate the results of the transcriptome analysis, we performed immunoflu ...
Infectious Hematopoietic Necrosis recombinant viruses induce
... cell culture, and to be virulent for rainbow trout by bath infection. The replacement of ...
... cell culture, and to be virulent for rainbow trout by bath infection. The replacement of ...
Maggie Lowe - McManus Lab
... transcriptional control was hypothesized to be the predominant regulatory mechanism for miRNA. However, discrepancies between the levels of primary transcript and mature miRNA have revealed that other cellular mechanisms downstream of transcription must also be active.3 Among these, inhibition of D ...
... transcriptional control was hypothesized to be the predominant regulatory mechanism for miRNA. However, discrepancies between the levels of primary transcript and mature miRNA have revealed that other cellular mechanisms downstream of transcription must also be active.3 Among these, inhibition of D ...
A Genetically Encoded Optical Neurotechnique Probe of Membrane
... whether a signal originates from electrical activity in neurons or glia, nor which types of neurons are involved. One general approach to this problem is to encode protein-based sensors into DNA. This permits the sensor to be placed under the control of cell-specific promoters and to be introduced i ...
... whether a signal originates from electrical activity in neurons or glia, nor which types of neurons are involved. One general approach to this problem is to encode protein-based sensors into DNA. This permits the sensor to be placed under the control of cell-specific promoters and to be introduced i ...
T-cell regulator RNF125/TRAC-1 belongs to a novel family of
... isolation. COS-7, HeLa and HEK-293T (human embryonic kidney) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) (Cambrex), 10 % (v/v) FCS, 100 units/ml penicillin and 0.1 mg/ml streptomycin. For transfection, 107 Jurkat T-cells were electroporated in 250 µl of RPMI 1640 medium containing ...
... isolation. COS-7, HeLa and HEK-293T (human embryonic kidney) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) (Cambrex), 10 % (v/v) FCS, 100 units/ml penicillin and 0.1 mg/ml streptomycin. For transfection, 107 Jurkat T-cells were electroporated in 250 µl of RPMI 1640 medium containing ...
Rapid movement of axonal neurofilaments interrupted by prolonged
... yielded conflicting results. The first study of this kind reported anterograde movement of tubulin in cultured PC12 cells 2, but subsequent studies on tubulin in PC12 cells3, cultured chick sensory neurons4 and developing neurons in grasshopper and zebrafish embryos5,6, as well as studies of tubulin ...
... yielded conflicting results. The first study of this kind reported anterograde movement of tubulin in cultured PC12 cells 2, but subsequent studies on tubulin in PC12 cells3, cultured chick sensory neurons4 and developing neurons in grasshopper and zebrafish embryos5,6, as well as studies of tubulin ...
Full Text - Plant and Cell Physiology
... CSLM, which was also followed by the quantitative analysis. Under the control conditions (0.1% DMSO), GFP–CESA3 was time-dependently internalized into the cytoplasm after hypotonic stimulus and predominantly localized in the PM and ringshaped compartments closely associated with the CLC– mOrange-lab ...
... CSLM, which was also followed by the quantitative analysis. Under the control conditions (0.1% DMSO), GFP–CESA3 was time-dependently internalized into the cytoplasm after hypotonic stimulus and predominantly localized in the PM and ringshaped compartments closely associated with the CLC– mOrange-lab ...
Genetically encoded phenyl azide photochemistry drives
... Subsequent products of the various nitrene reaction pathways can be used to alter protein structure and thus control function.8 ...
... Subsequent products of the various nitrene reaction pathways can be used to alter protein structure and thus control function.8 ...
PDF (Title Page, Abstract, Acknowledgements, Table of Contents
... muscles, intestinal epithelial cells, neurons, pharyngeal muscles), permitting proteins in those cells – and only those cells – to be labeled with azides. Labeled proteins are therefore subject to “click” conjugation to cyclooctyne-functionalized affinity probes, separation from the rest of the prot ...
... muscles, intestinal epithelial cells, neurons, pharyngeal muscles), permitting proteins in those cells – and only those cells – to be labeled with azides. Labeled proteins are therefore subject to “click” conjugation to cyclooctyne-functionalized affinity probes, separation from the rest of the prot ...
Abstract The family Solanaceae is unique in representing crops like
... forefront of scientific importance in the fields of comparative genomics and plastome biology research with other model organisms that are well studied, such as rice, Arabidopsis and tobacco, respectively. An understanding of the plastome biology of the Solanaceae makes this an ideal time to link pl ...
... forefront of scientific importance in the fields of comparative genomics and plastome biology research with other model organisms that are well studied, such as rice, Arabidopsis and tobacco, respectively. An understanding of the plastome biology of the Solanaceae makes this an ideal time to link pl ...
Protein folding
... bonds. This motif is named based on its similarity to the pattern seen on Greek pottery. This motif occurs frequently in β-barrel structures. ...
... bonds. This motif is named based on its similarity to the pattern seen on Greek pottery. This motif occurs frequently in β-barrel structures. ...
The stability and nuclear localization of the transcription factor RAP2
... inside an H85 HypOxistation (HypOxygen, Frederick, MD, USA) containing an artificial atmosphere consisting of oxygen and nitrogen in the proportions described in the text and figure legends. During the hypoxic and re-oxygenation treatments, plants were maintained in the dark at 22 °C. The time perio ...
... inside an H85 HypOxistation (HypOxygen, Frederick, MD, USA) containing an artificial atmosphere consisting of oxygen and nitrogen in the proportions described in the text and figure legends. During the hypoxic and re-oxygenation treatments, plants were maintained in the dark at 22 °C. The time perio ...
Characterization of Organelles in the Vacuolar
... We have shown the localization and mobilization of modified green fluorescent proteins (GFPs) with various signals in different compartments in a vacuolar-sorting system of tobacco BY-2 cells. In contrast to the efficient secretion of GFP from the transformed cells expressing SP-GFP composed of a si ...
... We have shown the localization and mobilization of modified green fluorescent proteins (GFPs) with various signals in different compartments in a vacuolar-sorting system of tobacco BY-2 cells. In contrast to the efficient secretion of GFP from the transformed cells expressing SP-GFP composed of a si ...
RNA Synthetic Biology
... Highly specific pockets in the 5′ UTR of the mRNAs that bind ligands conformational change in RNA structure change in ...
... Highly specific pockets in the 5′ UTR of the mRNAs that bind ligands conformational change in RNA structure change in ...
PDF - Walter Lab
... Microscopic analyses of pil1D cells showed that Pil1 is required for proper localization of both Lsp1–GFP (Fig. 2a) and Sur7–GFP (Fig. 2b): in the absence of Pil1, Lsp1–GFP localized to only a few bright clusters (less than four per cell) at the cell periphery and to a diffusely staining cytoplasmic ...
... Microscopic analyses of pil1D cells showed that Pil1 is required for proper localization of both Lsp1–GFP (Fig. 2a) and Sur7–GFP (Fig. 2b): in the absence of Pil1, Lsp1–GFP localized to only a few bright clusters (less than four per cell) at the cell periphery and to a diffusely staining cytoplasmic ...
Photolabeling of Proteins and Cells
... variant, we irradiated it with 413-nm laser light (8), which resulted in increased absorbance in the minor peak region (Fig. 1C, open squares). The fluorescence emission peak of the photoconverted T203H mutant was slightly red-shifted relative to WEGFP (Fig. 1D). Irradiation of several other T203 va ...
... variant, we irradiated it with 413-nm laser light (8), which resulted in increased absorbance in the minor peak region (Fig. 1C, open squares). The fluorescence emission peak of the photoconverted T203H mutant was slightly red-shifted relative to WEGFP (Fig. 1D). Irradiation of several other T203 va ...
Functions of LIM proteins in cell polarity and chemotactic motility
... In Dictyostelium cells, macropinocytosis accounts for most of the ¯uid-phase uptake and depends on the integrity of the actin cytoskeleton (Hacker et al., 1997). We studied the dynamics of GFP±LimC and GFP±LimD distribution in Dictyostelium cells during uptake of TRITC±dextran. At the beginning of t ...
... In Dictyostelium cells, macropinocytosis accounts for most of the ¯uid-phase uptake and depends on the integrity of the actin cytoskeleton (Hacker et al., 1997). We studied the dynamics of GFP±LimC and GFP±LimD distribution in Dictyostelium cells during uptake of TRITC±dextran. At the beginning of t ...
Technologie de l’ADN Recombinant CHMI 4226 F
... Expression in yeast – use of Pichia pastoris • P. pastoris: – Methylotrophic yeast: uses methanol as sole carbon source, yielding formaldehyde and hydrogen peroxide (done in peroxysomes); – Protein glycosylation is closer to mammalian cells; – A mich higher biomass (10 times!!) can be obtained with ...
... Expression in yeast – use of Pichia pastoris • P. pastoris: – Methylotrophic yeast: uses methanol as sole carbon source, yielding formaldehyde and hydrogen peroxide (done in peroxysomes); – Protein glycosylation is closer to mammalian cells; – A mich higher biomass (10 times!!) can be obtained with ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.