Zhen jin liprin protein regulates differentiation of presynmaptic termini c elegans nature 1999
... At synaptic junctions, specialized subcellular structures occur in both pre- and postsynaptic cells. Most presynaptic termini contain electron-dense membrane structures1, often referred to as active zones, which function in vesicle docking and release2. The components of those active zones and how t ...
... At synaptic junctions, specialized subcellular structures occur in both pre- and postsynaptic cells. Most presynaptic termini contain electron-dense membrane structures1, often referred to as active zones, which function in vesicle docking and release2. The components of those active zones and how t ...
Dynamic redox potential change throughout apoptosis in cancer
... intracellular redox potential and cell death is desirable since it could help us understand disease and responses to treatment. In this study we are investigating the dynamic change in redox potential throughout cell death. Apoptosis is characterized by a number of distinct morphological (chromatin ...
... intracellular redox potential and cell death is desirable since it could help us understand disease and responses to treatment. In this study we are investigating the dynamic change in redox potential throughout cell death. Apoptosis is characterized by a number of distinct morphological (chromatin ...
Introduction
... Turnover of cellular proteins was discovered in the 1930s in studies of Rudolf Schoenheimer, but it was in the 1960s that is became apparent that this was not just turnover, but a highly selective process. By the end of the 1970s two independent groups were working on two different topics: in the la ...
... Turnover of cellular proteins was discovered in the 1930s in studies of Rudolf Schoenheimer, but it was in the 1960s that is became apparent that this was not just turnover, but a highly selective process. By the end of the 1970s two independent groups were working on two different topics: in the la ...
Microscopy
... Green Fluorescent Protein (GFP) - A naturally occurring protein fluorescent probe derived from the jellyfish Aequorea victoria, which is commonly employed to determine the location, concentration, interactions, and dynamics of a target protein in living cells and tissues. The excitation and emission ...
... Green Fluorescent Protein (GFP) - A naturally occurring protein fluorescent probe derived from the jellyfish Aequorea victoria, which is commonly employed to determine the location, concentration, interactions, and dynamics of a target protein in living cells and tissues. The excitation and emission ...
An enhanced transient expression system in plants based on
... Transient gene expression is a fast, flexible and reproducible approach to high-level expression of useful proteins. In plants, recombinant strains of Agrobacterium tumefaciens can be used for transient expression of genes that have been inserted into the T-DNA region of the bacterial Ti plasmid. A ...
... Transient gene expression is a fast, flexible and reproducible approach to high-level expression of useful proteins. In plants, recombinant strains of Agrobacterium tumefaciens can be used for transient expression of genes that have been inserted into the T-DNA region of the bacterial Ti plasmid. A ...
FRET Results Conclusions Introduction Protein Interaction
... diabetes and certain forms of cancer. The study of these interactions has been significantly advanced by the discovery of the genetic structure of the green fluorescent protein (GFP) in the jellyfish Aequorea Victoria (1992), enabling fluorescent labelling. Furthermore, the introduction of mutations ...
... diabetes and certain forms of cancer. The study of these interactions has been significantly advanced by the discovery of the genetic structure of the green fluorescent protein (GFP) in the jellyfish Aequorea Victoria (1992), enabling fluorescent labelling. Furthermore, the introduction of mutations ...
Differential Expression Control and Polarized Distribution of Plasma
... expressed in certain root tissues of 5-day-old seedlings. The GFP fluorescence of SYP111/KNOLLE was detected exclusively in dividing cells in the root tip region (Fig. 1A). The majority of expressed SYP111 proteins were predominantly localized to the forming cell plates during cytokinesis (arrows in ...
... expressed in certain root tissues of 5-day-old seedlings. The GFP fluorescence of SYP111/KNOLLE was detected exclusively in dividing cells in the root tip region (Fig. 1A). The majority of expressed SYP111 proteins were predominantly localized to the forming cell plates during cytokinesis (arrows in ...
Fluorescence, confocal microscopy
... IgG or albumin that are conjugated with 5 or more fluorescein molecules, for example, fluoresce less than when bound to 2–3 molecules, because energy is transferred to nonfluorescent fluorescein dimers. Photobleaching refers to the permanent loss of fluorescence by a dye due to photon-induced chemic ...
... IgG or albumin that are conjugated with 5 or more fluorescein molecules, for example, fluoresce less than when bound to 2–3 molecules, because energy is transferred to nonfluorescent fluorescein dimers. Photobleaching refers to the permanent loss of fluorescence by a dye due to photon-induced chemic ...
The Arabidopsis Rab5 Homologs Rha1 and Ara7 Localize to the
... role in vacuolar trafficking in plant cells (Sohn et al. 2003). To advance our understanding of their role in vacuolar trafficking, we examined their localization. We used in vivo targeting approaches in protoplasts (Kim et al. 2001, Lee et al. 2002) using green fluorescent protein (GFP)-tagged fusi ...
... role in vacuolar trafficking in plant cells (Sohn et al. 2003). To advance our understanding of their role in vacuolar trafficking, we examined their localization. We used in vivo targeting approaches in protoplasts (Kim et al. 2001, Lee et al. 2002) using green fluorescent protein (GFP)-tagged fusi ...
Fluorescent High-Throughput Conjugation and Deconjugation
... intrachain ubiquitination assay format can be used. Since both the TR-FRET donor (Tbubiquitin) and acceptor (fluorescein-ubiquitin) are present in the polyubiquitin chain, no development step is required for the intrachain assay. This makes the intrachain assay especially useful when real-time kinet ...
... intrachain ubiquitination assay format can be used. Since both the TR-FRET donor (Tbubiquitin) and acceptor (fluorescein-ubiquitin) are present in the polyubiquitin chain, no development step is required for the intrachain assay. This makes the intrachain assay especially useful when real-time kinet ...
BioWire_Progress_Report_Week_One_Rev_1
... [Components will be ordered Monday] Replicate Weiss sender cell, adding GFP as a reporter Manually add aTc to the system in varying concentrations Control: Add water instead of aTc Expected Result: GFP is expressed, but not in control plates. ...
... [Components will be ordered Monday] Replicate Weiss sender cell, adding GFP as a reporter Manually add aTc to the system in varying concentrations Control: Add water instead of aTc Expected Result: GFP is expressed, but not in control plates. ...
Guidelines for Abstract Submission
... N-terminal region. In order to identify residues important for chloroplast and/or mitochondria targeting, we introduced point mutations and deletions into conserved residues of RBP1b TS, and evaluated their effect in the relative mitochondria/chloroplast targeting, using a novel GFP quantitative app ...
... N-terminal region. In order to identify residues important for chloroplast and/or mitochondria targeting, we introduced point mutations and deletions into conserved residues of RBP1b TS, and evaluated their effect in the relative mitochondria/chloroplast targeting, using a novel GFP quantitative app ...
In gram negative bacteria, Outer membrane proteins synthesized in
... various OMPs into the membrane. To gain a better insight into the mechanism, by which Skp binds its client proteins in the periplasm, we designed, expressed and isolated a new Skp construct, Sx3kp, from E. coli. In this construct, the three Skp monomers were linked together with two short and flexib ...
... various OMPs into the membrane. To gain a better insight into the mechanism, by which Skp binds its client proteins in the periplasm, we designed, expressed and isolated a new Skp construct, Sx3kp, from E. coli. In this construct, the three Skp monomers were linked together with two short and flexib ...
Document
... phase. Light that is unaltered hits the phase ring in the lens and is excluded. Light that is slightly altered by passing through different refractive index is allowed through. Light passing through cellular structures such as chromosomes or mitochondria is retarded because they have a higher refrac ...
... phase. Light that is unaltered hits the phase ring in the lens and is excluded. Light that is slightly altered by passing through different refractive index is allowed through. Light passing through cellular structures such as chromosomes or mitochondria is retarded because they have a higher refrac ...
Fluorescent Cell Imaging Activities for Your Classroom - Bio-Rad
... numerous times within the cytoplasm, generating a large number of nuclei. Approximately four hours after fertilization, after 13 mitotic divisions, an estimated 6,000 nuclei will organize themselves at the surface of the oocyte to create the syncytial blastoderm. Worms Feeding on GFP Bacteria This i ...
... numerous times within the cytoplasm, generating a large number of nuclei. Approximately four hours after fertilization, after 13 mitotic divisions, an estimated 6,000 nuclei will organize themselves at the surface of the oocyte to create the syncytial blastoderm. Worms Feeding on GFP Bacteria This i ...
Escherichia coli
... Integral membrane proteins in Escherichia coli cells Located in the inner membrane of cell wall Vital for cellular functions • Difficult to study Due to hydrophobic and amphiphilic nature Less than 1% of high resolution 3D structures known ...
... Integral membrane proteins in Escherichia coli cells Located in the inner membrane of cell wall Vital for cellular functions • Difficult to study Due to hydrophobic and amphiphilic nature Less than 1% of high resolution 3D structures known ...
INVESTIGATING REDOX POTENTIAL WITH REDOX
... cellular metabolism and external stimuli. In all cells, small molecules capable of thiol-disulphide exchange such as glutathione (GSH) are abundant and are thought to act as buffers in redox equilibrium [1]. Given the importance and the general interest in cellular redox processes, a noninvasive met ...
... cellular metabolism and external stimuli. In all cells, small molecules capable of thiol-disulphide exchange such as glutathione (GSH) are abundant and are thought to act as buffers in redox equilibrium [1]. Given the importance and the general interest in cellular redox processes, a noninvasive met ...
as a PDF
... splice was expressed predominantly in testis. The C2 splice had a more general expression pattern. Therefore, in addition to the distinct features revealed by amino acid sequences, these splice variants also were expressed in a tissue-specific manner. Because the N0 splice contained sequences shared ...
... splice was expressed predominantly in testis. The C2 splice had a more general expression pattern. Therefore, in addition to the distinct features revealed by amino acid sequences, these splice variants also were expressed in a tissue-specific manner. Because the N0 splice contained sequences shared ...
Introductory presentation(, 3.5 MB)
... FUNCTION FINDERS Discover how DNA sequences code for proteins with different roles and functions yourgenome.org ...
... FUNCTION FINDERS Discover how DNA sequences code for proteins with different roles and functions yourgenome.org ...
Observations of green fluorescent protein as a fusion partner in
... wherein the total yield and specific activity of the productportion of the fusion were decreased while the time course profiles were similar. Thus, the advantages in processing (upstream and downstream) which have been projected for the use of the GFP fusions (Albano et al., 1996; Poppenborg et al., ...
... wherein the total yield and specific activity of the productportion of the fusion were decreased while the time course profiles were similar. Thus, the advantages in processing (upstream and downstream) which have been projected for the use of the GFP fusions (Albano et al., 1996; Poppenborg et al., ...
Production and Purification of Recombinant Fluorescent Protein
... commonly employed to track the localization and dynamics of proteins, organelles, and other cellular compartments. A variety of techniques have been developed to construct fluorescent protein fusion products and enhance their expression in mammalian and other systems. The primary vehicles for introd ...
... commonly employed to track the localization and dynamics of proteins, organelles, and other cellular compartments. A variety of techniques have been developed to construct fluorescent protein fusion products and enhance their expression in mammalian and other systems. The primary vehicles for introd ...
Co-translational, Intraribosomal Cleavage of Polypeptides by the
... some uncleaved full-length proteins. Thus the FMDV 2A sequence is functional in yeast cells. Pulse-chase analysis (e.g. Fig. 2B) revealed that the proportion of full-length protein to cleavage product remained constant during the chase period, the amount of both reducing similarly over time, presuma ...
... some uncleaved full-length proteins. Thus the FMDV 2A sequence is functional in yeast cells. Pulse-chase analysis (e.g. Fig. 2B) revealed that the proportion of full-length protein to cleavage product remained constant during the chase period, the amount of both reducing similarly over time, presuma ...
Easy Transformation of E.coli Using GPF
... Easy Transformation of E.coli Using GPF 3. Obtain a 1.5 mL microcentrifuge tube and label it “–Control.” This tube will not receive any plasmid DNA. It is your negative control. 4. Add 200 µL of 51mM CaCl2 to each tube. Place both tubes in your beaker of ice. 5. Obtain an E.coli starter plate. Usin ...
... Easy Transformation of E.coli Using GPF 3. Obtain a 1.5 mL microcentrifuge tube and label it “–Control.” This tube will not receive any plasmid DNA. It is your negative control. 4. Add 200 µL of 51mM CaCl2 to each tube. Place both tubes in your beaker of ice. 5. Obtain an E.coli starter plate. Usin ...
Supplementary Methods
... the dendritic shaft to the outermost part of the spine head. For the determination of spine width, a line was drawn across the widest part of the dendritic protrusion (“spine head”) and the average pixel intensity of the GFP channel was derived along this line. Spine width was defined as the length ...
... the dendritic shaft to the outermost part of the spine head. For the determination of spine width, a line was drawn across the widest part of the dendritic protrusion (“spine head”) and the average pixel intensity of the GFP channel was derived along this line. Spine width was defined as the length ...
Lecture 1
... Example: The extinction coefficient of fluorescein (pH 9.5) is ~93,000 M-1cm–1 at 490nm The length of the cuvette is 1 cm. An absorption of 0.019 corresponds to a concentration of ~2 x 10 ...
... Example: The extinction coefficient of fluorescein (pH 9.5) is ~93,000 M-1cm–1 at 490nm The length of the cuvette is 1 cm. An absorption of 0.019 corresponds to a concentration of ~2 x 10 ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.