An in-depth look at fluorescent dyes for organelle labeling
... reagents that have been used and their characteristics. A featured reagent is highlighted, along with a validated protocol for that reagent with an estimate on protocol time, a troubleshooting guide in table format, an image example, and relevant recipes for stock and final solutions. In Part I the ...
... reagents that have been used and their characteristics. A featured reagent is highlighted, along with a validated protocol for that reagent with an estimate on protocol time, a troubleshooting guide in table format, an image example, and relevant recipes for stock and final solutions. In Part I the ...
4/3
... • The curves indicate the PCR cycle at which fluorescence is detected • Each cycle is twice the amount of the previous cycle ...
... • The curves indicate the PCR cycle at which fluorescence is detected • Each cycle is twice the amount of the previous cycle ...
Size exclusion chromatography with multi-angle
... microscope PSF in future fits. In order to identify fluorescent spots within cells, ...
... microscope PSF in future fits. In order to identify fluorescent spots within cells, ...
4_1 MI Quest_ ANSWER KEY - Kenwood Academy High School
... 34. Why does a salt buffer cause hydrophobic amino acids, like the ones in GFP, to push to the outside of a protein and bind to a chromatography column? (2 pts) A. The hydrophilic amino acids in GFP push to the inside of the column to be washed out B. The hydrophobic amino acids in GFP push to the i ...
... 34. Why does a salt buffer cause hydrophobic amino acids, like the ones in GFP, to push to the outside of a protein and bind to a chromatography column? (2 pts) A. The hydrophilic amino acids in GFP push to the inside of the column to be washed out B. The hydrophobic amino acids in GFP push to the i ...
A Nanobody-Based System Using Fluorescent Proteins as Scaffolds
... factors or site-specific recombinases in specific cell populations can then be used to control the expression of genes in responder cassettes. However, the utility of individual lines is limited by a transgene’s functional abilities; reporter lines expressing fluorescent proteins and histochemical e ...
... factors or site-specific recombinases in specific cell populations can then be used to control the expression of genes in responder cassettes. However, the utility of individual lines is limited by a transgene’s functional abilities; reporter lines expressing fluorescent proteins and histochemical e ...
Dual targeting of aminoacyl-tRNA synthetases to the mitochondrion
... Accepted 18 September 2012 Journal of Cell Science 125, 6176–6184 ß 2012. Published by The Company of Biologists Ltd doi: 10.1242/jcs.116533 ...
... Accepted 18 September 2012 Journal of Cell Science 125, 6176–6184 ß 2012. Published by The Company of Biologists Ltd doi: 10.1242/jcs.116533 ...
Template for Electronic Submission to ACS Journals - Lirias
... 8 is shown in tube representation and residues on β-strand 8 are represented by their side chains only. (c) Superposition of the red-on chromophores of pcDronpa (red), Kaede (grey) and IrisFP (yellow). Color code: oxygen in red; nitrogen in blue; carbon in the color corresponding with the color of t ...
... 8 is shown in tube representation and residues on β-strand 8 are represented by their side chains only. (c) Superposition of the red-on chromophores of pcDronpa (red), Kaede (grey) and IrisFP (yellow). Color code: oxygen in red; nitrogen in blue; carbon in the color corresponding with the color of t ...
Supplementary Information
... had the following sequence: 5’CTAATACGACTCACTATAGGGCGGCTGGTGAGATGGTTCAGT-3’. A second primer, containing only the suppression sequence underlined above, was named Supp. The two antisense primers designed on the viral vectors are: 5’-GCCTCAATAAAGCTTGCCTTG-3’, 5’TCCCAGGCTCAGATCTGGTCTAAC-3’, for the fi ...
... had the following sequence: 5’CTAATACGACTCACTATAGGGCGGCTGGTGAGATGGTTCAGT-3’. A second primer, containing only the suppression sequence underlined above, was named Supp. The two antisense primers designed on the viral vectors are: 5’-GCCTCAATAAAGCTTGCCTTG-3’, 5’TCCCAGGCTCAGATCTGGTCTAAC-3’, for the fi ...
pGLO workflow - Howard University > Plant Biotechnology
... Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria. Green Fluorescent Protein causes the jellyfish to fluoresce and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produ ...
... Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria. Green Fluorescent Protein causes the jellyfish to fluoresce and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produ ...
Magnetic relaxation switches capable of sensing molecular
... Highly sensitive, efficient, and high-throughput biosensors are required for genomic and proteomic data acquisition in complex biological samples and potentially for in vivo applications. To facilitate these studies, we have developed biocompatible magnetic nanosensors that act as magnetic relaxatio ...
... Highly sensitive, efficient, and high-throughput biosensors are required for genomic and proteomic data acquisition in complex biological samples and potentially for in vivo applications. To facilitate these studies, we have developed biocompatible magnetic nanosensors that act as magnetic relaxatio ...
- Wiley Online Library
... the GPI moiety. This revealed 248 potential GAPs that corresponded to a variety of proteins, including putative receptors, proteases, oxidases, extensins and lipid-transfer proteins. A proteomic analysis subsequently identified 39 of these proteins. Lipid-based sorting is obviously an area ripe for ...
... the GPI moiety. This revealed 248 potential GAPs that corresponded to a variety of proteins, including putative receptors, proteases, oxidases, extensins and lipid-transfer proteins. A proteomic analysis subsequently identified 39 of these proteins. Lipid-based sorting is obviously an area ripe for ...
Position Effect Variegation
... • Only some are directly involved • HP-1, Su(var)3-7 both co-localize to heterochromatin, interact in yeast two hybrid assay – Neither binds DNA ...
... • Only some are directly involved • HP-1, Su(var)3-7 both co-localize to heterochromatin, interact in yeast two hybrid assay – Neither binds DNA ...
User Manual - System Biosciences
... protein maturation, high stability at a wide range of pH (pH4-12), and does not require any additional cofactors or substrates. The copGFP protein has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emi ...
... protein maturation, high stability at a wide range of pH (pH4-12), and does not require any additional cofactors or substrates. The copGFP protein has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emi ...
A photoactivatable green-fluorescent protein from the phylum
... hydrozoan (Cnidaria) fluorescent proteins that were very similar to those we cloned from cDNA prepared from ctenophore specimens. We therefore now believe that the green-fluorescent proteins reported in our study are not from ctenophores, and were due to incorporation of cnidarian prey into the cten ...
... hydrozoan (Cnidaria) fluorescent proteins that were very similar to those we cloned from cDNA prepared from ctenophore specimens. We therefore now believe that the green-fluorescent proteins reported in our study are not from ctenophores, and were due to incorporation of cnidarian prey into the cten ...
SIZE EXCLUSION AND HI CHROMATOGRAPHY
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development • Formation of different organs • Screenable marker to identify transgenic organisms ...
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development • Formation of different organs • Screenable marker to identify transgenic organisms ...
Farnesyl diphosphate synthase, the target for nitrogen
... The mevalonate pathway of isoprenoid biosynthesis allows eukaryotic cells to convert acetyl-CoA into FDP (farnesyl diphosphate). FDP is mainly used for biosynthesis of sterols but it may also be used, either directly or after conversion into geranylgeranyl diphosphate, for protein prenylation [1]. T ...
... The mevalonate pathway of isoprenoid biosynthesis allows eukaryotic cells to convert acetyl-CoA into FDP (farnesyl diphosphate). FDP is mainly used for biosynthesis of sterols but it may also be used, either directly or after conversion into geranylgeranyl diphosphate, for protein prenylation [1]. T ...
GFP Assays: Live–Cell Translocation Assays
... In collaboration with BioImage, Amersham Biosciences has developed a set of translocation assays. These live-cell assays can be used to track protein movements within intra-cellular pathways and highlight any effects caused by potential drug candidates. They also allow you to detect more specific ag ...
... In collaboration with BioImage, Amersham Biosciences has developed a set of translocation assays. These live-cell assays can be used to track protein movements within intra-cellular pathways and highlight any effects caused by potential drug candidates. They also allow you to detect more specific ag ...
A1985ALF1000002
... Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178:871-2, 1972. [Roche Inst. Mol. Biol , and Chem. Res. Dept , Hoffmann-La Roche, Inc., Nutley, NJ] me that he considered the proposed mechanism invalid and asked me to hold off ...
... Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178:871-2, 1972. [Roche Inst. Mol. Biol , and Chem. Res. Dept , Hoffmann-La Roche, Inc., Nutley, NJ] me that he considered the proposed mechanism invalid and asked me to hold off ...
Molecular Biology – Final Laboratory Report
... The gene expression profile of the JAL1 gene indicated that JAL1 is transcribed at low levels 4 hours post-conjugation, and at higher levels 8 hours after conjugation. In order to identify possible processes which the product of the JAL1 gene is involved in, it is necessary to consider what was goin ...
... The gene expression profile of the JAL1 gene indicated that JAL1 is transcribed at low levels 4 hours post-conjugation, and at higher levels 8 hours after conjugation. In order to identify possible processes which the product of the JAL1 gene is involved in, it is necessary to consider what was goin ...
Laser Sources for Life Sciences Research
... stable tagging of cells with a fluorescent probe, allowing later detection after injection into a laboratory animal. CellTracker Red from Invitrogen is one such proteinbinding dye that requires orange excitation. In fact, because red HeNe and diode lasers do not provide good excitation of this probe ...
... stable tagging of cells with a fluorescent probe, allowing later detection after injection into a laboratory animal. CellTracker Red from Invitrogen is one such proteinbinding dye that requires orange excitation. In fact, because red HeNe and diode lasers do not provide good excitation of this probe ...
Transcriptional Activity of Male Gamete
... of the 35S promoter in pollen (Twell et al. 1991, Okada and Toriyama 2001). Use of pollen vegetative cell-specific promoters such as LAT52 (Twell et al. 1990) and Bra r 1 (Okada and Toriyama 2001) resulted in a much higher ratio of GFP expressed pollen (T. Okada unpublished data). Together with thes ...
... of the 35S promoter in pollen (Twell et al. 1991, Okada and Toriyama 2001). Use of pollen vegetative cell-specific promoters such as LAT52 (Twell et al. 1990) and Bra r 1 (Okada and Toriyama 2001) resulted in a much higher ratio of GFP expressed pollen (T. Okada unpublished data). Together with thes ...
Transformations
... Like the lux genes, the GFP gene has an aquatic origin. GFP stands for Green Fluorescent Protein, and the GFP gene is from a bioluminescent jellyfish, Aequorea victoria. These jellyfish emit a green glow from the edges of their belllike structures. This glow is easily seen in the coastal waters inha ...
... Like the lux genes, the GFP gene has an aquatic origin. GFP stands for Green Fluorescent Protein, and the GFP gene is from a bioluminescent jellyfish, Aequorea victoria. These jellyfish emit a green glow from the edges of their belllike structures. This glow is easily seen in the coastal waters inha ...
A Conditional System to Specifically Link Disruption of
... deletion of a highly expressed target transcript. Finally, recent reports of abundant circular RNA species comprised of exons of protein-coding genes with potential additional activity forewarn of further inadvertent changes in cellular state when transcripts are entirely ablated or truncated (Memcz ...
... deletion of a highly expressed target transcript. Finally, recent reports of abundant circular RNA species comprised of exons of protein-coding genes with potential additional activity forewarn of further inadvertent changes in cellular state when transcripts are entirely ablated or truncated (Memcz ...
Genomewide view of gene silencing by small interfering RNAs
... machinery for generating siRNA also appears to be used for the production of a second class of endogenously encoded, small RNA molecules termed microRNAs (miRNAs). miRNA are processed from endogenous transcripts that form hairpin structures, and miRNAs are thought to mediate the translational contro ...
... machinery for generating siRNA also appears to be used for the production of a second class of endogenously encoded, small RNA molecules termed microRNAs (miRNAs). miRNA are processed from endogenous transcripts that form hairpin structures, and miRNAs are thought to mediate the translational contro ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.