IL-13 - York College of Pennsylvania
... IL-13 ligand has been experimentally attached to other proteins to produce a new fusion or ‘chimera’ protein. By successfully developing a fused protein consisting of more than one active part, researchers have been able to successfully develop a single protein with multiple functions and potential ...
... IL-13 ligand has been experimentally attached to other proteins to produce a new fusion or ‘chimera’ protein. By successfully developing a fused protein consisting of more than one active part, researchers have been able to successfully develop a single protein with multiple functions and potential ...
Culturing A. castellanii - College of Science
... protozoa Acanthamoeba castellanii. These eukaryotes of clade Amoebozoa are a species of gymnamoeba (naked amoeba) that fills niches in diverse aquatic and soil environments. These organisms are also opportunistic pathogens of humans and are associated with certain pathogenic and non-pathogenic bacte ...
... protozoa Acanthamoeba castellanii. These eukaryotes of clade Amoebozoa are a species of gymnamoeba (naked amoeba) that fills niches in diverse aquatic and soil environments. These organisms are also opportunistic pathogens of humans and are associated with certain pathogenic and non-pathogenic bacte ...
Chloroplast Tubules Visualized in Transplastomic Plants Expressing
... during chloroplast development between tobacco and Arabisopsis. Recently, Kohler and Hanson (2000) reported that the appearance of the plastid tubules (stromules) are abundant in root cells. However, we could not observe them due to the low level GFP expression from the leafspecific psbA promoter in ...
... during chloroplast development between tobacco and Arabisopsis. Recently, Kohler and Hanson (2000) reported that the appearance of the plastid tubules (stromules) are abundant in root cells. However, we could not observe them due to the low level GFP expression from the leafspecific psbA promoter in ...
Repressilator
... Experimental overview. The first attemp. The mathematical model. Determination of the appropiate parameters. ...
... Experimental overview. The first attemp. The mathematical model. Determination of the appropiate parameters. ...
Chapter 15
... For each of the following sentences, fill in the blanks with the best word or phrase selected from the list below. Not all words or phrases will be used; each word or phrase should be used only once. Many features of __________________ cells make them suitable for biochemical studies of the cell-cyc ...
... For each of the following sentences, fill in the blanks with the best word or phrase selected from the list below. Not all words or phrases will be used; each word or phrase should be used only once. Many features of __________________ cells make them suitable for biochemical studies of the cell-cyc ...
Aph-1 and pen-2 are required for Notch pathway
... compound E induces Notch pathway phenotypes in Drosophila and C. elegans.(A) Untreated wild-type Drosophila wing.(B) Wing from an animal raised on compound E (40 l of 5 mg/ml solution in DMSO placed on food surface).(C) One gonad arm of a C. elegans hop-1(ep171) hermaphrodite raised on compound E (1 ...
... compound E induces Notch pathway phenotypes in Drosophila and C. elegans.(A) Untreated wild-type Drosophila wing.(B) Wing from an animal raised on compound E (40 l of 5 mg/ml solution in DMSO placed on food surface).(C) One gonad arm of a C. elegans hop-1(ep171) hermaphrodite raised on compound E (1 ...
Endocytosis-like protein uptake in the bacterium Gemmata
... formation of vesicle structures in the cells allowing extracellular molecules to be internalized. Remarkably, proteins possessing structural features of eukaryotic MC proteins have been recently described in planctomycetes (23). MC proteins are related to the clathrin and coat protein families, all ...
... formation of vesicle structures in the cells allowing extracellular molecules to be internalized. Remarkably, proteins possessing structural features of eukaryotic MC proteins have been recently described in planctomycetes (23). MC proteins are related to the clathrin and coat protein families, all ...
Supplementary Figure 1. Experimental electron density
... frozen crystal with an ADSC Quantum Q210 CCD detector at beamline X25 at the National Synchrotron Light Source, Brookhaven National Laboratory. The crystals belong to space group C2 with unit cell dimensions of a = 82.60, b = 95.17, c = 65.57 Å, = 96.81˚. All data were processed and scaled using H ...
... frozen crystal with an ADSC Quantum Q210 CCD detector at beamline X25 at the National Synchrotron Light Source, Brookhaven National Laboratory. The crystals belong to space group C2 with unit cell dimensions of a = 82.60, b = 95.17, c = 65.57 Å, = 96.81˚. All data were processed and scaled using H ...
Transform cells and spread plates
... o The presence of any colonies on the amp plate would suggest that those bacteria are resistant to the antibiotic ampicillin ...
... o The presence of any colonies on the amp plate would suggest that those bacteria are resistant to the antibiotic ampicillin ...
Transport of virally expressed green fluorescent protein through the
... peptide (Boevink et al. 1996). Fluorescence was restricted to the ER, which had two predominant distributions: (i) in a labile cortical network of tubules with occasional small lamellae (Fig. 3E,F) and (ii) in bright transvacuolar strands (not shown). Within both these systems small motile ¯uorescen ...
... peptide (Boevink et al. 1996). Fluorescence was restricted to the ER, which had two predominant distributions: (i) in a labile cortical network of tubules with occasional small lamellae (Fig. 3E,F) and (ii) in bright transvacuolar strands (not shown). Within both these systems small motile ¯uorescen ...
Intracellular Distribution of an Integral Nuclear Pore Membrane
... nucleotide sequences encoding the selected regions of POM121 Fig. 4. Intracellular distribution POM121-GFP chimeras and delewith 24 additional amino acids originating from a different read- tion mutants. Equatorial sections of nuclei of COS-I cells studied by ing frame. The coding sequences were cut ...
... nucleotide sequences encoding the selected regions of POM121 Fig. 4. Intracellular distribution POM121-GFP chimeras and delewith 24 additional amino acids originating from a different read- tion mutants. Equatorial sections of nuclei of COS-I cells studied by ing frame. The coding sequences were cut ...
LAB 6 pGlo Powerpoint - Bremen High School District 228
... resistance gene or be modified to contain other genes ...
... resistance gene or be modified to contain other genes ...
Probes for Multiplexed Detection of GFP
... the localization of a GFP fusion to SPoIIIE, a protein essential for translocation of the forespore chromosome that may also regulate membrane fusion events (see Proc Natl Acad Sci U S A 96, 14553 (1999)). The background contains sporangia at various stages in the engulfment process stained with Mit ...
... the localization of a GFP fusion to SPoIIIE, a protein essential for translocation of the forespore chromosome that may also regulate membrane fusion events (see Proc Natl Acad Sci U S A 96, 14553 (1999)). The background contains sporangia at various stages in the engulfment process stained with Mit ...
magnetic GFP-Trap -M for Immunoprecipitation of GFP
... magnetic GFP-Trap®-M for Immunoprecipitation of GFP-Fusion Proteins (gtm) For the immunoprecipitation of GFP-fusion-proteins from cellular extracts. Only for research applications, not for diagnostic or therapeutic use 1. Introduction Green fluorescent proteins (GFP) and variants thereof are widely ...
... magnetic GFP-Trap®-M for Immunoprecipitation of GFP-Fusion Proteins (gtm) For the immunoprecipitation of GFP-fusion-proteins from cellular extracts. Only for research applications, not for diagnostic or therapeutic use 1. Introduction Green fluorescent proteins (GFP) and variants thereof are widely ...
synthetic gene networks that count
... GFP fluorescence ratios between the single-inducer DIC three-counter exposed to three pulses of arabinose (N) versus two pulses of arabinose (N – 1) with varying arabinose pulse lengths and intervals; experimental results are represented by black dots. ...
... GFP fluorescence ratios between the single-inducer DIC three-counter exposed to three pulses of arabinose (N) versus two pulses of arabinose (N – 1) with varying arabinose pulse lengths and intervals; experimental results are represented by black dots. ...
學生: 徐綜遠 - 分子與細胞生物學研究所 - National Taiwan University
... Tg(KY43-3) embryos are GFP-positive, and thus induced by heat shock stress, HuC and GFAP immunostaining were performed. Results showed that the GFP-positive cells were not HuC-positive neuron cells, but rather, partial GFAP-positive glia cells, suggesting that some glia cells and immature neural ste ...
... Tg(KY43-3) embryos are GFP-positive, and thus induced by heat shock stress, HuC and GFAP immunostaining were performed. Results showed that the GFP-positive cells were not HuC-positive neuron cells, but rather, partial GFAP-positive glia cells, suggesting that some glia cells and immature neural ste ...
Transformation Lab - Central Magnet School
... jellyfish gene that codes for GFP. When bacteria that have been transformed with pGLO plasmid DNA are grown in the presence of arabinose, the GFP gene is turned on and the bacteria glow brilliant green when exposed to UV light. ...
... jellyfish gene that codes for GFP. When bacteria that have been transformed with pGLO plasmid DNA are grown in the presence of arabinose, the GFP gene is turned on and the bacteria glow brilliant green when exposed to UV light. ...
The green fluorescent protein: discovery
... about 100Å, and the method has been generalized to involve energy transfer between three, rather than two, GFP chromophores. Engineering of GFP from Aequorea victoria has led to improved brightness and photo-stability. In addition, variants with better folding properties at temperatures higher than ...
... about 100Å, and the method has been generalized to involve energy transfer between three, rather than two, GFP chromophores. Engineering of GFP from Aequorea victoria has led to improved brightness and photo-stability. In addition, variants with better folding properties at temperatures higher than ...
TCS Cellworks and Essen have entered into a strategic relationship
... Under the terms of the agreement, TCS will distribute IncuCyte™-compatible AngioKit’s™ in Europe, and Essen Instruments will distribute the same kits in North America. In addition, TCS and Essen will co-market a “GFP Angiokit” for use in association with the IncuCyteFLR imaging system. The GFP label ...
... Under the terms of the agreement, TCS will distribute IncuCyte™-compatible AngioKit’s™ in Europe, and Essen Instruments will distribute the same kits in North America. In addition, TCS and Essen will co-market a “GFP Angiokit” for use in association with the IncuCyteFLR imaging system. The GFP label ...
Seminar L11- Laboratorija za molekularno biologijo in
... This presentation gives a brief overview on Microscale Thermophoresis (MST), a new technology to study biomolecule interactions. The term Microscale Thermophoresis refers to the directed movement of molecules in optically generated microscopic temperature gradients. This thermophoretic movement is d ...
... This presentation gives a brief overview on Microscale Thermophoresis (MST), a new technology to study biomolecule interactions. The term Microscale Thermophoresis refers to the directed movement of molecules in optically generated microscopic temperature gradients. This thermophoretic movement is d ...
Cells in the Microscope Biol 497B Bioimaging 1 Cells in the
... encoding green fluorescent protein (GFP) a naturally occurring fluorescent protein isolated from jelly fish Aequorea victoria (Chalfie et al., 1994). With this sequence, nearly any protein for which a gene sequence is available can be fluorescently tagged by creating a chimeric gene consisting of th ...
... encoding green fluorescent protein (GFP) a naturally occurring fluorescent protein isolated from jelly fish Aequorea victoria (Chalfie et al., 1994). With this sequence, nearly any protein for which a gene sequence is available can be fluorescently tagged by creating a chimeric gene consisting of th ...
Automated Microscopy, Machine Learning, Systems Biology, and
... (without using colocalization!) Examination of proteins for which methods disagree suggests machine classifier is correct in at least some cases Shann-Ching (Sam) Chen & Geoff Gordon ...
... (without using colocalization!) Examination of proteins for which methods disagree suggests machine classifier is correct in at least some cases Shann-Ching (Sam) Chen & Geoff Gordon ...
- Flintbox
... emerged and their increasing prevalence in otherwise healthy individuals is a matter of great clinical concern. Optimal host response to infection with S. aureus relies on the efficient ingestion and killing of microorganisms. ...
... emerged and their increasing prevalence in otherwise healthy individuals is a matter of great clinical concern. Optimal host response to infection with S. aureus relies on the efficient ingestion and killing of microorganisms. ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.