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Staphylococcus Aureus Strain with GFP/YFP/mCherry Fluorescence Technology Primer: An estimated 32% of the US population is colonized with Staphylococcus aureus, with the highest prevalence among children. The proportion of strains that is methicillin resistant is increasing, with 64% of screened isolates from hospital intensive care units testing positive for methicillin resistance. In recent years, more virulent community-associated methicillin-resistant strains of S. aureus have emerged and their increasing prevalence in otherwise healthy individuals is a matter of great clinical concern. Optimal host response to infection with S. aureus relies on the efficient ingestion and killing of microorganisms. Technology Description: Researchers at the University of Iowa have developed a strain of Staphylococcus aureus that constitutively expresses GFP, YFP, or mCherry protein. These clonal strains maintain their plasmid due to selection media and have been shown to generate strong fluorescent signals when exposed to excitatory light wavelengths. These strains can be easily incorporated into research studies requiring rapid detection of growth or clearing of small amounts of bacteria or small changes in growth or growth rate. Additional Technology Benefit: Plasmids can also be removed from the strains of Staphylococcus aureus and used as a vector to create customized strains of bacteria. This enables one to screen compounds that are capable of killing strains that are resistant to other antibiotics.