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Study guide Microbiology Dr. Yoga Sundram Procaryotes Eucaryotes Viruses Unicellular No nucleus No organelles Metabolism Uni or multicellular Nucleus present Organelles present Metabolism Acellular (protein particles) Nucleoid (DNA only) No organelles Parasitic Scientific contributions Antonie van Leeuwenhoek: First to observe living microbes using lenses John Tyndall & Ferdinand Cohn: Demonstrated endospores resistant to heat Oliver Wendell Holmes: Suggested nosocomial (hospital acquired) infections Joseph Lister: Introduced aseptic techniques Louis pasteur: Disproved spontaneous theory Introduced germ theory Developed pasteurization Developed rabies vaccine Robert Koch: Koch's postulate Isolated and identified TB, anthrax and cholera Spontaneous theory: Living things arose from non living things spontaneously Evolution: All new organisms originated from existing organism: They were more advanced They were more complex They came through a process of "survival of the fittest". Classification: Kingdom, Phylum, Class, Order, Family, Genus, Species Naming bacteria: Either or and not or Staphylococcus aureus Staphylococcus aureus Staphylococcus Aureus staphylococcus aureus Genera upper case Species lower case Italicized or Underlined