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Transcript
Seminar L11- Laboratorija za molekularno biologijo in nanobiotehnologijo in Infrastrukturnega centra za raziskave molekulskih interakcij, Oddelek za biologijo, Biotehniška fakulteta, Univerza v Ljubljani Beate Kern NanoTemper Technologies GmbH, Munich, Germany Biomolecular Interaction Analytics with NanoTemper Technologies Seminar bo v torek 14. 4. 2015 ob 9:30. uri v Preglovi predavalnici (PRC) Kemijskega inštituta Povzetek seminarja This presentation gives a brief overview on Microscale Thermophoresis (MST), a new technology to study biomolecule interactions. The term Microscale Thermophoresis refers to the directed movement of molecules in optically generated microscopic temperature gradients. This thermophoretic movement is determined by the entropy of the hydration shell around the molecules. Microscale Thermophoresis allows quantification of binding affinities of protein-, nucleic acid- and small molecule-interactions. The steep microscopic temperature gradient is generated by an IR-Laser, which is strongly absorbed by water. The readout method of the interaction analysis is based on fluorescence: fluorescently labeled proteins/peptides/nucleic acids can be used as well as GFP/YFP/RFP fusion-proteins. In this presentation we will briefly describe the technical details and the benefits of the Microscale Thermophoresis technology platform – the aspects will be given as a published examples of groups that used the technique with the emphasis of membrane protein interactions. In addition the new technologies, NanoDSF and SAW, will be presented as a novel approaches of protein characterization. VLJUDNO VABLJENI!