Distinguishing coding from non-coding sequences in a prokaryote
... ftp://ncbi.nlm.nih.gov/genbank/genomes/, many research works have been carried out on genomic sequences. Recognition of coding sequences in a genome contributes to the automatic genome annotation process. So there is a demand for prediction techniques that can rapidly and accurately distinguish the ...
... ftp://ncbi.nlm.nih.gov/genbank/genomes/, many research works have been carried out on genomic sequences. Recognition of coding sequences in a genome contributes to the automatic genome annotation process. So there is a demand for prediction techniques that can rapidly and accurately distinguish the ...
Transgenerational Epigenetic Inheritance in Plants - 文献云下载
... broadly defined as the “change in gene expression without base sequence alteration” (Riggs and Porter 1996). This is frequently found during somatic cell differentiation in animal cells, typically occurring in clonal expansion of a single cell, leading to a diversity of cell types (Holliday 1993). A ...
... broadly defined as the “change in gene expression without base sequence alteration” (Riggs and Porter 1996). This is frequently found during somatic cell differentiation in animal cells, typically occurring in clonal expansion of a single cell, leading to a diversity of cell types (Holliday 1993). A ...
Ecological Risks of Gene Drive Technologies
... Box 2. Harnessing DNA repair All organisms have mechanisms to repair or destroy faulty molecules. When DNA is damaged by a break or cut, the cell’s machinery has broadly two methods to repair it. One is to re-join the two broken ends, known as non-homologous end-joining (NHEJ). NHEJ is error-prone a ...
... Box 2. Harnessing DNA repair All organisms have mechanisms to repair or destroy faulty molecules. When DNA is damaged by a break or cut, the cell’s machinery has broadly two methods to repair it. One is to re-join the two broken ends, known as non-homologous end-joining (NHEJ). NHEJ is error-prone a ...
Discovery of MLL1 binding units, their localization to CpG Islands
... Background: Mixed Lineage Leukemia 1 (MLL1) is a mammalian ortholog of the Drosophila Trithorax. In Drosophila, Trithorax complexes transmit the memory of active genes to daughter cells through interactions with Trithorax Response Elements (TREs). However, despite their functional importance, nothin ...
... Background: Mixed Lineage Leukemia 1 (MLL1) is a mammalian ortholog of the Drosophila Trithorax. In Drosophila, Trithorax complexes transmit the memory of active genes to daughter cells through interactions with Trithorax Response Elements (TREs). However, despite their functional importance, nothin ...
Chpt11_TxnPromoters.doc
... The methods for making cDNA from mRNA are more prone to copy the 3’ ends and middle of mRNAs than the 5’ ends. Thus it is common to have access to this part of the cDNA, and that provides the sequence information for the second, or internal, primer. In contrast, specialized techniques are often empl ...
... The methods for making cDNA from mRNA are more prone to copy the 3’ ends and middle of mRNAs than the 5’ ends. Thus it is common to have access to this part of the cDNA, and that provides the sequence information for the second, or internal, primer. In contrast, specialized techniques are often empl ...
The genetic basis of evolutionary change in gene expression levels
... the evolution of form as spectacularly evidenced through the study of development (Carroll 2008), the relationship between genetics and development was at one time a yawning gulf. The rise of the authority of Mendelian genetics ushered in by T.H. Morgan led to a view where genes were treated as trai ...
... the evolution of form as spectacularly evidenced through the study of development (Carroll 2008), the relationship between genetics and development was at one time a yawning gulf. The rise of the authority of Mendelian genetics ushered in by T.H. Morgan led to a view where genes were treated as trai ...
Protein expression in plastids Peter B Heifetz* and Ann Marie Tuttle
... for their correct interaction with the polymerase [24]. Plastid genes can have only PEP promoters, only NEP promoters, or hybrid promoter regions that contain both PEP and NEP elements. The significance for message accumulation of these multiple transcription initiation sites is unclear. A recent st ...
... for their correct interaction with the polymerase [24]. Plastid genes can have only PEP promoters, only NEP promoters, or hybrid promoter regions that contain both PEP and NEP elements. The significance for message accumulation of these multiple transcription initiation sites is unclear. A recent st ...
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes
... equal amount of DNA from six F2 plants showing resistant and susceptible phenotypes, respectively. Polymerase chain reaction of markers was performed in a PE9600 thermal cycler (PerkinElmer) in a volume of 10 mL containing 10 to 20 ng of template, 2 pmol of each of the primers, 2 nmol of each of the ...
... equal amount of DNA from six F2 plants showing resistant and susceptible phenotypes, respectively. Polymerase chain reaction of markers was performed in a PE9600 thermal cycler (PerkinElmer) in a volume of 10 mL containing 10 to 20 ng of template, 2 pmol of each of the primers, 2 nmol of each of the ...
Pulling forces acting on Hox gene clusters cause expression
... Antennapedia, numerous homologue genes were found in many other genomes. These were named Hox genes and it turns out that they are grouped in complexes called HOX clusters. As in the Drosophila, Hox gene mutations cause severe homeotic transformations and malformations of the embryonic body plan. Be ...
... Antennapedia, numerous homologue genes were found in many other genomes. These were named Hox genes and it turns out that they are grouped in complexes called HOX clusters. As in the Drosophila, Hox gene mutations cause severe homeotic transformations and malformations of the embryonic body plan. Be ...
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes
... equal amount of DNA from six F2 plants showing resistant and susceptible phenotypes, respectively. Polymerase chain reaction of markers was performed in a PE9600 thermal cycler (PerkinElmer) in a volume of 10 mL containing 10 to 20 ng of template, 2 pmol of each of the primers, 2 nmol of each of the ...
... equal amount of DNA from six F2 plants showing resistant and susceptible phenotypes, respectively. Polymerase chain reaction of markers was performed in a PE9600 thermal cycler (PerkinElmer) in a volume of 10 mL containing 10 to 20 ng of template, 2 pmol of each of the primers, 2 nmol of each of the ...
Document
... Figure 1 The CRISPR/Cas9 system.1 Clustered regularly interspaced palindromic repeats (CRISPR) refers to sequences in the bacterial genome. They afford protection against invading viruses, when combined with a series of CRISPR-associated (Cas) proteins. Cas9, one of the associated proteins, is an en ...
... Figure 1 The CRISPR/Cas9 system.1 Clustered regularly interspaced palindromic repeats (CRISPR) refers to sequences in the bacterial genome. They afford protection against invading viruses, when combined with a series of CRISPR-associated (Cas) proteins. Cas9, one of the associated proteins, is an en ...
Recognition of an organism from fragments of its complete genome
... distinguish bacteria because the computed fractal dimensions of bacteria 关22兴 are the same. The classification and evolution relationship of bacteria is one of the most important problems in DNA research. Yu and Anh 关23兴 proposed a time series model based on the global structure of the complete geno ...
... distinguish bacteria because the computed fractal dimensions of bacteria 关22兴 are the same. The classification and evolution relationship of bacteria is one of the most important problems in DNA research. Yu and Anh 关23兴 proposed a time series model based on the global structure of the complete geno ...
A new repetitive DNA sequence family in the olive (Olea
... 81 bp family element (KATSIOTISet al. 1998). BITONTIet al. (1999) have described the OeTaq80 repeats with significant sequence homology with the 81 bp family. Sequence comparison between repeated DNA of Olea europaea cv. “Koroneiki”, and the first family of repeated DNA of Olea europaea cv. “Picual” ...
... 81 bp family element (KATSIOTISet al. 1998). BITONTIet al. (1999) have described the OeTaq80 repeats with significant sequence homology with the 81 bp family. Sequence comparison between repeated DNA of Olea europaea cv. “Koroneiki”, and the first family of repeated DNA of Olea europaea cv. “Picual” ...
REVIEW ARTICLE Regulation of Expression of the Integrated
... The great majority of these proviruses are transcriptionally silent; a few are activated under certain specified conditions, and others are permanently inactive and never able to specify transcripts. This lack of activity is perhaps not unexpected, since these proviruses exist as tolerated endo-para ...
... The great majority of these proviruses are transcriptionally silent; a few are activated under certain specified conditions, and others are permanently inactive and never able to specify transcripts. This lack of activity is perhaps not unexpected, since these proviruses exist as tolerated endo-para ...
The degenerate Y chromosome – can
... RBMY evolved from a ubiquitously expressed partner RBMX on the X (Delbridge et al. 1999; Mazeyrat et al. 1999), as did the testis-specific TSPY from a ubiquitously expressed TSPX (Delbridge et al. in press) and USP9Y, shown to be required for spermatogenesis in human beings (Sun et al. 1999), and Ub ...
... RBMY evolved from a ubiquitously expressed partner RBMX on the X (Delbridge et al. 1999; Mazeyrat et al. 1999), as did the testis-specific TSPY from a ubiquitously expressed TSPX (Delbridge et al. in press) and USP9Y, shown to be required for spermatogenesis in human beings (Sun et al. 1999), and Ub ...
Isolation of a gene encoding a novel chloroplast protein by T
... the C-terminal amino acids of the wild-type protein were replaced by 11 new codons (Figure 4). A DNA sequence of 16 bp located at the T-DNA -plant DNA junction showed homology to neither T-DNA nor the deleted target site. At the T-DNA boundaries 21 bp from the left and 3 bp from the right 25 bp bord ...
... the C-terminal amino acids of the wild-type protein were replaced by 11 new codons (Figure 4). A DNA sequence of 16 bp located at the T-DNA -plant DNA junction showed homology to neither T-DNA nor the deleted target site. At the T-DNA boundaries 21 bp from the left and 3 bp from the right 25 bp bord ...
Full Text
... prediction because of their higher conservation when compared with equivalent DNA sequences; (ii) it minimizes the number of false positives caused by sometimes inconsistent hits ranking produced by local sequence alignment tools such as BLAST; and (iii) it ensures that sequence similarities obtaine ...
... prediction because of their higher conservation when compared with equivalent DNA sequences; (ii) it minimizes the number of false positives caused by sometimes inconsistent hits ranking produced by local sequence alignment tools such as BLAST; and (iii) it ensures that sequence similarities obtaine ...
AthaMap web tools for database-assisted identification of
... the PlantProm database (7) together with the positional information relative to transcription start sites (TSSs) or translation start sites. Because each TFBS is associated with a particular score that represents the similarity of the site to the underlying alignment matrix, a new functionality was ...
... the PlantProm database (7) together with the positional information relative to transcription start sites (TSSs) or translation start sites. Because each TFBS is associated with a particular score that represents the similarity of the site to the underlying alignment matrix, a new functionality was ...
genstat - University of Illinois at Urbana
... • TestCode finds ORFs/Exons based on compositional bias with a periodicity of three ...
... • TestCode finds ORFs/Exons based on compositional bias with a periodicity of three ...
Acute stress and hippocampal histone H3 lysine 9 trimethylation, a
... but it was associated with reductions (stress down) at only 1,438 loci (Fig. 3A) [false-discovery rate (FDR) <0.0001, P < 0.05]. When categorized by repetitive-element class, differences emerge in the enrichment of stress-up versus stress-down peaks: the autonomous long interspersed element (LINE) r ...
... but it was associated with reductions (stress down) at only 1,438 loci (Fig. 3A) [false-discovery rate (FDR) <0.0001, P < 0.05]. When categorized by repetitive-element class, differences emerge in the enrichment of stress-up versus stress-down peaks: the autonomous long interspersed element (LINE) r ...
lecture05_11
... • When searching for a motif in a genome using PSSM or other methods – the motif is usually found all over the place ->The motif is considered real if found in the vicinity of a gene. • Checking experimentally for the binding sites of a specific TF (location analysis) – the sites that bind the motif ...
... • When searching for a motif in a genome using PSSM or other methods – the motif is usually found all over the place ->The motif is considered real if found in the vicinity of a gene. • Checking experimentally for the binding sites of a specific TF (location analysis) – the sites that bind the motif ...
Reference genome sequence of the model plant Setaria
... For full genome analysis, nuclear DNA was prepared from Yugu1 seedlings and subjected to Sanger sequence analysis on ABI3730xl capillary sequencing machines. Separate libraries with several insert sizes (3 kb, 6 kb, 37 kb, 121 kb) were end-sequenced using standard Sanger-based methods. A total of 5, ...
... For full genome analysis, nuclear DNA was prepared from Yugu1 seedlings and subjected to Sanger sequence analysis on ABI3730xl capillary sequencing machines. Separate libraries with several insert sizes (3 kb, 6 kb, 37 kb, 121 kb) were end-sequenced using standard Sanger-based methods. A total of 5, ...
PPT - Glasnost
... `Yeast' has a gene count of 6000 `Thale cress' has a gene count of 26000 -----------------------------------------------------------`Fruit fly' has a gene count of 13000 `Human' has a gene count of 31000 `Nematode worm' has a gene count of 18000 `Thale cress' has a gene count of 26000 `Tuberculosis ...
... `Yeast' has a gene count of 6000 `Thale cress' has a gene count of 26000 -----------------------------------------------------------`Fruit fly' has a gene count of 13000 `Human' has a gene count of 31000 `Nematode worm' has a gene count of 18000 `Thale cress' has a gene count of 26000 `Tuberculosis ...
Evolution of genetic and genomic features unique to the human
... Large cytogenetically visible changes in the genome structure were among the first human-specific genomic changes noted between humans and great apes. More recently, it has been determined that these regions harbour more importance than just being human-specific heterochromatin. Indeed, such regions ...
... Large cytogenetically visible changes in the genome structure were among the first human-specific genomic changes noted between humans and great apes. More recently, it has been determined that these regions harbour more importance than just being human-specific heterochromatin. Indeed, such regions ...
second of three for Chapter 8
... When an inversion involves the centromere, it is called a pericentric inversion. Crossing over in a pericentric inversion does not create dicentric and acentric chromosomes ...
... When an inversion involves the centromere, it is called a pericentric inversion. Crossing over in a pericentric inversion does not create dicentric and acentric chromosomes ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.