Simplified Insertion of Transgenes Onto Balancer Chromosomes via
... inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE). ...
... inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE). ...
Systems-wide Chicken DNA Microarrays, Gene Expression Profiling
... sequences in the UD collection. About 24% of our EST without previous identification are now in contigs with a high Blast score, whereas 17% are in contigs with a low Blast score. Although 33% of our EST are still classified as unknown genes, they are now assembled in highfidelity contigs, which pro ...
... sequences in the UD collection. About 24% of our EST without previous identification are now in contigs with a high Blast score, whereas 17% are in contigs with a low Blast score. Although 33% of our EST are still classified as unknown genes, they are now assembled in highfidelity contigs, which pro ...
The Complete Genome Sequence of Clostridium aceticum: a
... spectroscopy at room temperature and ⫺196°C in cell-free extracts of C. aceticum (15). The bands obtained indicated a c-type cytochrome. Indeed, all genes required for synthesis of a cytochrome c could be identified in the genome of C. aceticum (hemACDBL [CACET_c00470-CACET_c00520], hemE [CACET_ c22 ...
... spectroscopy at room temperature and ⫺196°C in cell-free extracts of C. aceticum (15). The bands obtained indicated a c-type cytochrome. Indeed, all genes required for synthesis of a cytochrome c could be identified in the genome of C. aceticum (hemACDBL [CACET_c00470-CACET_c00520], hemE [CACET_ c22 ...
Diplosporous development in Boehmeria tricuspis: Insights
... we established that triploid B. tricuspis reproduces through obligate diplospory. To understand the molecular basis of apomictic development in B. tricuspis, we sequenced and compared transcriptomic profiles of the flowers of sexual and apomictic plants at four key developmental stages. A total of 2 ...
... we established that triploid B. tricuspis reproduces through obligate diplospory. To understand the molecular basis of apomictic development in B. tricuspis, we sequenced and compared transcriptomic profiles of the flowers of sexual and apomictic plants at four key developmental stages. A total of 2 ...
Genomic imprinting of a placental lactogen gene in Peromyscus
... organism. For this reason there has been intense debate about the function of imprinting (Moore and Haig 1991; Hurst 1997). While the more than 30 imprinted genes discovered to date have a variety of biochemical functions and many are widely expressed, mutations in the genes disproportionately affec ...
... organism. For this reason there has been intense debate about the function of imprinting (Moore and Haig 1991; Hurst 1997). While the more than 30 imprinted genes discovered to date have a variety of biochemical functions and many are widely expressed, mutations in the genes disproportionately affec ...
A Molecularly Defined Duplication Set for the X Chromosome of
... Transformation: DNA was injected into y1 M{vas-int.Dm}ZH2A w*;PBac{y1-attP-3B}VK00033 embryos. Adult flies were crossed to five w1118;TM2/TM6C,Sb. Initially, we transferred the adults to fresh vials three times and screened the G1 progeny for mini-white expressing transformants once a week for 3 wee ...
... Transformation: DNA was injected into y1 M{vas-int.Dm}ZH2A w*;PBac{y1-attP-3B}VK00033 embryos. Adult flies were crossed to five w1118;TM2/TM6C,Sb. Initially, we transferred the adults to fresh vials three times and screened the G1 progeny for mini-white expressing transformants once a week for 3 wee ...
14 Phylogenomic Approach to the Evolutionary Dynamics of Gene
... diverge to produce novel functions in a process known as neofunctionalization (Zhang, 2003). For example, some duplicated members of the RNaseA gene superfamily in primates evolved a novel antibacterial function that was not present in the common ancestral gene or its descendants (Zhang et al., 1998 ...
... diverge to produce novel functions in a process known as neofunctionalization (Zhang, 2003). For example, some duplicated members of the RNaseA gene superfamily in primates evolved a novel antibacterial function that was not present in the common ancestral gene or its descendants (Zhang et al., 1998 ...
The DNA sequence of human chromosome 21
... and pseudogenes. The catalogue was arbitrarily divided into ®ve main hierarchical categories (see below) to distinguish known genes from pure gene predictions, and also anonymous complementary DNA sequences from those exhibiting similarities to known proteins or modular domains. The criteria governi ...
... and pseudogenes. The catalogue was arbitrarily divided into ®ve main hierarchical categories (see below) to distinguish known genes from pure gene predictions, and also anonymous complementary DNA sequences from those exhibiting similarities to known proteins or modular domains. The criteria governi ...
Ge´nie: literature-based gene prioritization at multi genomic scale
... relation to a research topic. However many genes, especially from poorly studied organisms, are not discussed in the literature. Moreover, a manual and comprehensive summarization of the literature attached to the genes of an organism is in general impossible due to the high number of genes and abst ...
... relation to a research topic. However many genes, especially from poorly studied organisms, are not discussed in the literature. Moreover, a manual and comprehensive summarization of the literature attached to the genes of an organism is in general impossible due to the high number of genes and abst ...
Genome Projector: zoomable genome map with multiple views
... scales. The Zoomable User Interface (ZUI) and tabbed browsing have proven successful for this purpose in other areas, especially to navigate the vast information in the World Wide Web. Results: This paper presents Genome Projector, a Web-based gateway for genomics information with a zoomable user in ...
... scales. The Zoomable User Interface (ZUI) and tabbed browsing have proven successful for this purpose in other areas, especially to navigate the vast information in the World Wide Web. Results: This paper presents Genome Projector, a Web-based gateway for genomics information with a zoomable user in ...
induction of instability at selected loci in maize
... Chromosomal segments capable of moving from one position on the chromosome to another within the genome. The genome is not static; genes have the ability to move. The normal genetic role of this is unknown, but is a universal phenomenon and can be found in many different organisms, including: plants ...
... Chromosomal segments capable of moving from one position on the chromosome to another within the genome. The genome is not static; genes have the ability to move. The normal genetic role of this is unknown, but is a universal phenomenon and can be found in many different organisms, including: plants ...
Genetic and Epigenetic Aspects of Polyploid Evolution in Plants
... chimeric genes found in neither progenitor diploid. If these results from Arabidopsis and Gossypium are generalized, one can envision how allopolyploids have a greater likelihood for multivalent pairing, homoeologous exchanges and genomic rearrangements, all of which generate diversity by novel mech ...
... chimeric genes found in neither progenitor diploid. If these results from Arabidopsis and Gossypium are generalized, one can envision how allopolyploids have a greater likelihood for multivalent pairing, homoeologous exchanges and genomic rearrangements, all of which generate diversity by novel mech ...
References - UTH e
... Because of its rapidity and simplicity, PCR is ideally suited to providing numerous DNA templates for mutation screening. Partial DNA sequences, at the genomic or the cDNA level, from a gene associated with disease, or some other interesting phenotype, immediately enable gene-specific PCR reactions ...
... Because of its rapidity and simplicity, PCR is ideally suited to providing numerous DNA templates for mutation screening. Partial DNA sequences, at the genomic or the cDNA level, from a gene associated with disease, or some other interesting phenotype, immediately enable gene-specific PCR reactions ...
A Resurrection of B Chromosomes?
... How are genes of interest introduced onto engineered minichromosomes? Targeted transgene integration into unique chromosomal loci might be achieved using gene constructs in combination with a site-specific recombinase cassette as provided by the Cre/lox system. The proof of principle has been demons ...
... How are genes of interest introduced onto engineered minichromosomes? Targeted transgene integration into unique chromosomal loci might be achieved using gene constructs in combination with a site-specific recombinase cassette as provided by the Cre/lox system. The proof of principle has been demons ...
Genes and RNA
... Ribosomal RNAs (rRNAs) are components of ribosomes, which are large macromolecular assemblies that act as guides to coordinate the assembly of the amino acid chain of a protein. Ribosomes are composed of several types of rRNA and about 100 different proteins. As in the case of tRNA, the rRNAs are ge ...
... Ribosomal RNAs (rRNAs) are components of ribosomes, which are large macromolecular assemblies that act as guides to coordinate the assembly of the amino acid chain of a protein. Ribosomes are composed of several types of rRNA and about 100 different proteins. As in the case of tRNA, the rRNAs are ge ...
Rare genomic changes and mitochondrial sequences
... number of non-canonical tRNA structures have been found within arachnid mt genomes (Masta and Boore, 2008), but their structures have also proven to be phylogenetically informative within some groups, such as ticks (Murrell et al., 2003) and spiders (Masta and Boore, 2008). Besides potentially provi ...
... number of non-canonical tRNA structures have been found within arachnid mt genomes (Masta and Boore, 2008), but their structures have also proven to be phylogenetically informative within some groups, such as ticks (Murrell et al., 2003) and spiders (Masta and Boore, 2008). Besides potentially provi ...
Allele- and parent-of-origin-specific effects on expression of the
... 2009), while ASE is common in non-imprinted loci throughout the genome (Tycko, 2010). Our results are consistent with those from other studies in humans (Locke et al., 2015), in which the KCNJ11 gene was found to be in allelic imbalance in beta cells. Even though this pattern is consistent between b ...
... 2009), while ASE is common in non-imprinted loci throughout the genome (Tycko, 2010). Our results are consistent with those from other studies in humans (Locke et al., 2015), in which the KCNJ11 gene was found to be in allelic imbalance in beta cells. Even though this pattern is consistent between b ...
to the reprint.
... Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, MD , 1,3Department of Ophthalmology and Visual Sciences and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO Purpose: To identify homeobox-containing genes that may play a rol ...
... Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, MD , 1,3Department of Ophthalmology and Visual Sciences and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO Purpose: To identify homeobox-containing genes that may play a rol ...
The Chicken Gene Map
... others 1996a,b). It should be possible to define all microchromosomes using a set of reference probes within the next 3 yr. A World Wide Web (WWW1) page is maintained at the Roslin Institute (Edinburgh, United Kingdom) as part of the European CHICKMAP project to share information and probes for phys ...
... others 1996a,b). It should be possible to define all microchromosomes using a set of reference probes within the next 3 yr. A World Wide Web (WWW1) page is maintained at the Roslin Institute (Edinburgh, United Kingdom) as part of the European CHICKMAP project to share information and probes for phys ...
Archaeal Transcription Initiation - IMBB
... of the eucaryal TBP and TFIIB transcription initiation factors (Bult et al., 1996). Consistent with this, these are the only archaeal transcription factors needed to direct accurate transcription initiation in vitro by archaeal RNA polymerases (Thomm, 1996). Intriguingly, despite the usual complexit ...
... of the eucaryal TBP and TFIIB transcription initiation factors (Bult et al., 1996). Consistent with this, these are the only archaeal transcription factors needed to direct accurate transcription initiation in vitro by archaeal RNA polymerases (Thomm, 1996). Intriguingly, despite the usual complexit ...
A whole-genome assembly of the domestic cow, Bos taurus
... Another indicator of assembly completeness, and also of its potential for annotation, is the extent to which known gene sequences can be mapped onto it. We aligned 8,689 independently validated full-length cow mRNA sequences to the two assemblies, using spliced alignment mapping tools (see Materials ...
... Another indicator of assembly completeness, and also of its potential for annotation, is the extent to which known gene sequences can be mapped onto it. We aligned 8,689 independently validated full-length cow mRNA sequences to the two assemblies, using spliced alignment mapping tools (see Materials ...
My favourite flowering image: a cob of pod corn
... trait of the Solanaceae. Heinz’s intuition was right and, since he had teamed up with co-workers that were on a faster publication track, the initial work on the inflated calyx phenomenon was published many years before our work on Tunicate (He and Saedler, 2005). While the inflated calyx story gene ...
... trait of the Solanaceae. Heinz’s intuition was right and, since he had teamed up with co-workers that were on a faster publication track, the initial work on the inflated calyx phenomenon was published many years before our work on Tunicate (He and Saedler, 2005). While the inflated calyx story gene ...
The insect cytochrome oxidase I gene: evolutionary
... to 228 for COll and 261 for COlll; Clary & Wolstenholme, 1985), and is one of the largest proteincoding genes in the metazoan mitochondrial genome. This enables one to amplify and sequence many more characters (nucleotides), within the same functional complex, than is possible for almost any other m ...
... to 228 for COll and 261 for COlll; Clary & Wolstenholme, 1985), and is one of the largest proteincoding genes in the metazoan mitochondrial genome. This enables one to amplify and sequence many more characters (nucleotides), within the same functional complex, than is possible for almost any other m ...
FEBS Letters
... E. coli [9] and related sequences of hypothetical proteins in Bacillus subtilis and Synechocystis sp. PCC 6803 was performed. This led us to design suitable oligonucleotides (1for, 1rev; see Section 2) to amplify a 1085 bp segment of S. leopoliensis SAUG 1402-1 DNA in a PCR reaction. The deduced ami ...
... E. coli [9] and related sequences of hypothetical proteins in Bacillus subtilis and Synechocystis sp. PCC 6803 was performed. This led us to design suitable oligonucleotides (1for, 1rev; see Section 2) to amplify a 1085 bp segment of S. leopoliensis SAUG 1402-1 DNA in a PCR reaction. The deduced ami ...
E.Publication
... could be expensive. Should government pay for it? Maybe you say yes. However, the cost of this treatment for people who are albino may increase government’s expenditure on health for everyone. Would that change your answer? Think about the choices Fahim’s mother would have to make. If she loves Fahi ...
... could be expensive. Should government pay for it? Maybe you say yes. However, the cost of this treatment for people who are albino may increase government’s expenditure on health for everyone. Would that change your answer? Think about the choices Fahim’s mother would have to make. If she loves Fahi ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.