2007-10_GO-resources_jblake
... conjunction with the ontologies. The structures of the annotation files can be seen in an example here http://www.geneontology.org/GO.annotation.fields.shtml Click on ‘Annotation Files and Downloads’ [see arrow in screen shot]to look at files available for the model organism genomes. Notice that the ...
... conjunction with the ontologies. The structures of the annotation files can be seen in an example here http://www.geneontology.org/GO.annotation.fields.shtml Click on ‘Annotation Files and Downloads’ [see arrow in screen shot]to look at files available for the model organism genomes. Notice that the ...
Interfering RNA
... – probe function alone for target not sufficient to provide utility for RNAi ...
... – probe function alone for target not sufficient to provide utility for RNAi ...
Evolutionary dynamics of full genome content in Escherichia coli
... sequences are often acquired from organisms of very ...
... sequences are often acquired from organisms of very ...
The expression of a chromoplast-specific lycopene beta cyclase
... the b cyclase enzyme (Cunningham et al., 1994; Hugueney et al., 1995; Pecker et al., 1996). The e and b cyclase enzymes are products of related genes, but the e cyclase only adds one e ring to lycopene, whereas the b cyclase can add two b rings. Carotenoids with two e rings are not commonly found in ...
... the b cyclase enzyme (Cunningham et al., 1994; Hugueney et al., 1995; Pecker et al., 1996). The e and b cyclase enzymes are products of related genes, but the e cyclase only adds one e ring to lycopene, whereas the b cyclase can add two b rings. Carotenoids with two e rings are not commonly found in ...
Characterization of the first cultured representative of
... movies. Illumina sequencing was performed on a MiSeq platform with 2 × 150 cycles. The paired-end library contained inserts of an average insert size of 500 bp and delivered 1.8 million reads. A draft long read genome assembly named L21-Fru-AB-SP4_HGAP_5SC_std_np was created using the ‘RS_HGAP_Assem ...
... movies. Illumina sequencing was performed on a MiSeq platform with 2 × 150 cycles. The paired-end library contained inserts of an average insert size of 500 bp and delivered 1.8 million reads. A draft long read genome assembly named L21-Fru-AB-SP4_HGAP_5SC_std_np was created using the ‘RS_HGAP_Assem ...
Studies on Chlamydomonas Chloroplast Transformation: Foreign
... have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have measured transient expression of the chloramphenicol acetyltransferase gene in intact maize cells ...
... have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have measured transient expression of the chloramphenicol acetyltransferase gene in intact maize cells ...
Generation of the chromosome sequences
... Supplemental Segmental Duplication Analysis Segmental duplications have long been noted for their potential role in the evolution of new genes12. To examine the transcriptional and coding potential of duplicated regions, we analyzed a non-overlapping set of known genes with or without additional spl ...
... Supplemental Segmental Duplication Analysis Segmental duplications have long been noted for their potential role in the evolution of new genes12. To examine the transcriptional and coding potential of duplicated regions, we analyzed a non-overlapping set of known genes with or without additional spl ...
Comparative Genomics of Microbes
... • Tandem repeats: regions of repeated DNA in immediate succession but with different copy number in different genomes. – A repeat can occur 2.5 times October 2K5 ...
... • Tandem repeats: regions of repeated DNA in immediate succession but with different copy number in different genomes. – A repeat can occur 2.5 times October 2K5 ...
Shastry, B.S. 2002. SNP alleles in human disease and evolution
... SNPs in gene discovery Once the map of these SNPs is confirmed, they can be used for evolutionary biology studies, gene discovery and mapping, prediction of drug and environmental response, diagnostic tests, heterogeneity testing, and association studies (Gray et al. 2000; Schork et al. 2000). For t ...
... SNPs in gene discovery Once the map of these SNPs is confirmed, they can be used for evolutionary biology studies, gene discovery and mapping, prediction of drug and environmental response, diagnostic tests, heterogeneity testing, and association studies (Gray et al. 2000; Schork et al. 2000). For t ...
CapeTownGenomes
... Few genomes are completely sequenced. The completion and quality assurance needed for bacterial genomes is expensive, for larger eukaryotes even more so. ‘Finishing’ is the process by which a WGS shotgun assembly is completed (determine the sequence from any physical or sequence gaps) and furthe ...
... Few genomes are completely sequenced. The completion and quality assurance needed for bacterial genomes is expensive, for larger eukaryotes even more so. ‘Finishing’ is the process by which a WGS shotgun assembly is completed (determine the sequence from any physical or sequence gaps) and furthe ...
Alternative Splicing A very short introduction (in plants)
... Genome-wide analyses of alternative splicing in plants: Opportunities and challenges Genome Res. 2008. 18:1381-1392 ...
... Genome-wide analyses of alternative splicing in plants: Opportunities and challenges Genome Res. 2008. 18:1381-1392 ...
Reconstruction of a 450-My-old ancestral vertebrate protokaryotype
... orthologous loci map to a single chromosome in each of two or more different species, irrespective of conserved gene order [32]. Conserved synteny defines characteristics of ancestral chromosomes. This approach has been shown to be especially informative when integrating fish genome data, which show ...
... orthologous loci map to a single chromosome in each of two or more different species, irrespective of conserved gene order [32]. Conserved synteny defines characteristics of ancestral chromosomes. This approach has been shown to be especially informative when integrating fish genome data, which show ...
1 Title: Evidence for large domains of similarly expressed genes in
... We wished to know if there was a correlation between gene groups identified in the adult, embryo, and combined datasets. To do this we tabulated all genes identified in each dataset at each of three p-values (10-2, 10-3, 10-4) and calculated the Pearson correlation between each dataset at each p-val ...
... We wished to know if there was a correlation between gene groups identified in the adult, embryo, and combined datasets. To do this we tabulated all genes identified in each dataset at each of three p-values (10-2, 10-3, 10-4) and calculated the Pearson correlation between each dataset at each p-val ...
Warren, ST and Nelson, DL: Trinucleotide repeat expansions in neurological disease. Current Opinion in Neurobiology 3:752-759 (1993).
... genetic disease have followed rules and examples set forth in model systems such as Drosophila and yeast. In recent years, however, new mechanisms responsible for genetic disease have emerged where little or no precedent had been established in other genetically studied organisms. One such mechanism ...
... genetic disease have followed rules and examples set forth in model systems such as Drosophila and yeast. In recent years, however, new mechanisms responsible for genetic disease have emerged where little or no precedent had been established in other genetically studied organisms. One such mechanism ...
ppt_II
... Automatically finds genes and other features of the sequence Associates sequence and features with data from other sources Provides a publicly accessible web based interface to the database ...
... Automatically finds genes and other features of the sequence Associates sequence and features with data from other sources Provides a publicly accessible web based interface to the database ...
Information Encoding in Biological Molecules: DNA and
... Automatically finds genes and other features of the sequence Associates sequence and features with data from other sources Provides a publicly accessible web based interface to the database ...
... Automatically finds genes and other features of the sequence Associates sequence and features with data from other sources Provides a publicly accessible web based interface to the database ...
Non contiguous-finished genome sequence and description of
... Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [2] using a Microflex spectrometer (Bruker Daltonics, Leipzig, Germany). Twelve distinct deposits were done for strain JCET from 12 isolated colonies. The twelve JCET sp ...
... Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [2] using a Microflex spectrometer (Bruker Daltonics, Leipzig, Germany). Twelve distinct deposits were done for strain JCET from 12 isolated colonies. The twelve JCET sp ...
- LSHTM Research Online
... These were constructed by the insertion of a rhamnose-inducible promoter upstream of the predicted open reading frame (ORF). This results in the target gene being transcribed only in the presence of L-rhamnose, while transcription is abolished in the presence of glucose. Thus, if bacterial growth is ...
... These were constructed by the insertion of a rhamnose-inducible promoter upstream of the predicted open reading frame (ORF). This results in the target gene being transcribed only in the presence of L-rhamnose, while transcription is abolished in the presence of glucose. Thus, if bacterial growth is ...
Modular proteins I
... Original theory was that exons corresponded directly to modules and/or structural motifs ...
... Original theory was that exons corresponded directly to modules and/or structural motifs ...
Standard Mutation Nomenclature in Molecular Diagnostics
... As shown in Table 1, genetic sequence changes occur at the DNA level, and we usually identify mutations at the DNA level in a clinical genetic testing. Descriptions at the amino acid level are usually inferred with no experimental proof and are not unequivocal because amino acid codes are degenerate ...
... As shown in Table 1, genetic sequence changes occur at the DNA level, and we usually identify mutations at the DNA level in a clinical genetic testing. Descriptions at the amino acid level are usually inferred with no experimental proof and are not unequivocal because amino acid codes are degenerate ...
Driscoll Katee Driscoll Dr. Ely Genetics October 20, 2013 Effects of
... tightly together at more places and will thus be harder to separate. Based on these results, Duzdevich et al. postulated that unusual DNA structures as formed by super long CAG repeats reduced transcription levels and led to a reduced phenotypic expression as well. While Lee et al. provided evidence ...
... tightly together at more places and will thus be harder to separate. Based on these results, Duzdevich et al. postulated that unusual DNA structures as formed by super long CAG repeats reduced transcription levels and led to a reduced phenotypic expression as well. While Lee et al. provided evidence ...
Divergence Pattern of Duplicate Genes in Protein
... model. This model predicts symmetry in the divergence pattern of the duplicates under the assumption of an equal rate of new partner gains for two copies of one duplicate pair as well as a constant rate of interaction turnover for all duplicate pairs; that is,. two copies of one duplicate pair would ...
... model. This model predicts symmetry in the divergence pattern of the duplicates under the assumption of an equal rate of new partner gains for two copies of one duplicate pair as well as a constant rate of interaction turnover for all duplicate pairs; that is,. two copies of one duplicate pair would ...
Statistical analysis of simple repeats in the human genome
... Experiments on kinetics of DNA denaturation and renaturation and the analysis of DNA sequences have revealed that most of our genome is populated by DNA repeats of different length, number and degree of dispersion [1]. Long repeats in few copies are usually orthologous genes, which may contain hidde ...
... Experiments on kinetics of DNA denaturation and renaturation and the analysis of DNA sequences have revealed that most of our genome is populated by DNA repeats of different length, number and degree of dispersion [1]. Long repeats in few copies are usually orthologous genes, which may contain hidde ...
A Separate Peace – Pre
... Although Gene does not want to attend meetings of the “Super Suicide Society,” he attends the meetings, stating, “Otherwise, I would have lost face with Phineas, and that would have been unthinkable.” What does this tell us about Gene’s attitude towards Phineas? How important is Phineas to Gene’ ...
... Although Gene does not want to attend meetings of the “Super Suicide Society,” he attends the meetings, stating, “Otherwise, I would have lost face with Phineas, and that would have been unthinkable.” What does this tell us about Gene’s attitude towards Phineas? How important is Phineas to Gene’ ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.