Gene Section IGH@ (Immunoglobulin Heavy) Atlas of Genetics and Cytogenetics
... whereas 41 pseudogenes, which are too divergent to be assigned to subgroups, have been assigned to 4 clans. Seven non-mapped IGHV genes have been described as insertion/deletion polymorphism but have not yet been precisely located. The most 5' IGHV genes occupy a position very close to the chromosom ...
... whereas 41 pseudogenes, which are too divergent to be assigned to subgroups, have been assigned to 4 clans. Seven non-mapped IGHV genes have been described as insertion/deletion polymorphism but have not yet been precisely located. The most 5' IGHV genes occupy a position very close to the chromosom ...
genetic mapping and dna sequencing of the loblolly pine genome
... understanding of the organization and evolution of pine genomes. Two genetic maps were constructed using restriction fragment length polymorphism (RFLP) markers using complementary DNA (cDNA) probes from loblolly pine. Three generation outbred pedigrees were used for segregation and linkage analysis ...
... understanding of the organization and evolution of pine genomes. Two genetic maps were constructed using restriction fragment length polymorphism (RFLP) markers using complementary DNA (cDNA) probes from loblolly pine. Three generation outbred pedigrees were used for segregation and linkage analysis ...
Novel Molecular Methods for Discovery and Engineering of
... genes. If the properties are different from those of the known biocatalysts available in the databases, it means that the cloned genes and corresponding biocatalysts are newly found, which are not reported yet previously. In the case that a functional gene is located in the same single DNA insert as ...
... genes. If the properties are different from those of the known biocatalysts available in the databases, it means that the cloned genes and corresponding biocatalysts are newly found, which are not reported yet previously. In the case that a functional gene is located in the same single DNA insert as ...
Substitution Rates in a New Silene latifolia Sex
... relaxed purifying selection in Y-linked genes, leading to the accumulation of nonsynonymous substitutions and genetic degeneration of the Y-linked genes. ...
... relaxed purifying selection in Y-linked genes, leading to the accumulation of nonsynonymous substitutions and genetic degeneration of the Y-linked genes. ...
Gene ORGANizer: linking genes to the organs they
... if certain anatomical parts are over-represented in the ontology (because some phenotypes are easier to detect, or some diseases are more studied), it would not bias the results (2). Gene ORGANizer also allows users to enter their own background list. User-specified backgrounds are useful in cases w ...
... if certain anatomical parts are over-represented in the ontology (because some phenotypes are easier to detect, or some diseases are more studied), it would not bias the results (2). Gene ORGANizer also allows users to enter their own background list. User-specified backgrounds are useful in cases w ...
Identification of the Minus-Dominance Gene Ortholog in
... and skin tissue) after the cells had been homogenized with ceramic beads and a wash brush (Nozaki et al. 1997, 2006). Full-length cDNA synthesis from the total RNA was carried out with the CapFishing full-length cDNA premix kit (Seegene, Seoul, Korea). Nested RT–PCR using this cDNA yielded the parti ...
... and skin tissue) after the cells had been homogenized with ceramic beads and a wash brush (Nozaki et al. 1997, 2006). Full-length cDNA synthesis from the total RNA was carried out with the CapFishing full-length cDNA premix kit (Seegene, Seoul, Korea). Nested RT–PCR using this cDNA yielded the parti ...
Genome engineering of mammalian haploid embryonic stem cells
... (NGG) and the 12 bp seed sequence at the 30 end of the sgRNA (Jinek et al., 2012). The importance of the remaining bases is less well understood and may depend on the binding strength of the matching sgRNA or the inherent tolerance of Cas9 itself. Therefore, we selected a 23-mer sequence (N21GG) fro ...
... (NGG) and the 12 bp seed sequence at the 30 end of the sgRNA (Jinek et al., 2012). The importance of the remaining bases is less well understood and may depend on the binding strength of the matching sgRNA or the inherent tolerance of Cas9 itself. Therefore, we selected a 23-mer sequence (N21GG) fro ...
The Engineered Chloroplast Genome Just Got Smarter
... Chloroplast genome-engineering projects have led to stable integration and expression of transgenes from different kingdoms including bacterial, viral, fungal, animal, and human genes to express biopharmaceutical proteins, antibiotics, vaccine antigens, industrial enzymes, and biomaterials to conf ...
... Chloroplast genome-engineering projects have led to stable integration and expression of transgenes from different kingdoms including bacterial, viral, fungal, animal, and human genes to express biopharmaceutical proteins, antibiotics, vaccine antigens, industrial enzymes, and biomaterials to conf ...
The Mobile Genetic Element Alu in the Human Genome
... long, are similar to one another but not identical, and they are interspersed along the genome (not tandemly arrayed). Within this category are the trans po sons, elements that are able to move from one genomic location to another. This movement can be mediated by DNA or RNA. In RNA-mediated transpo ...
... long, are similar to one another but not identical, and they are interspersed along the genome (not tandemly arrayed). Within this category are the trans po sons, elements that are able to move from one genomic location to another. This movement can be mediated by DNA or RNA. In RNA-mediated transpo ...
Tutorial - Maize Inflorescence
... RNAseq: To know whether the selected genes are differentially expressed. ChIPseq: To query for presence of Peak summits within 2kb of the gene. SNPs: To know if there is any SNPs within 2kb of the gene. Orthologs: To get the Orthologs Save list: To save list of gene ids in a file ...
... RNAseq: To know whether the selected genes are differentially expressed. ChIPseq: To query for presence of Peak summits within 2kb of the gene. SNPs: To know if there is any SNPs within 2kb of the gene. Orthologs: To get the Orthologs Save list: To save list of gene ids in a file ...
Request pdf - University of Cambridge
... other primate species, we have looked at the MW and LW opsin gene sequences of two additional species of Great Ape, the gorilla, Gorilla gorilla, and chimpanzee, Pan troglodytes, and three species of Cercopithecoid monkeys. Except for the gorilla, all of these species are predominantly frugivorous i ...
... other primate species, we have looked at the MW and LW opsin gene sequences of two additional species of Great Ape, the gorilla, Gorilla gorilla, and chimpanzee, Pan troglodytes, and three species of Cercopithecoid monkeys. Except for the gorilla, all of these species are predominantly frugivorous i ...
S010
... microRNAs. Here, one transcript may be processed to produce multiple functional microRNA sequences. In addition, each microRNA precursor hairpin has the potential to produce two different mature microRNAs. We have investigated the evolutionary and functional relationships between the multiple produc ...
... microRNAs. Here, one transcript may be processed to produce multiple functional microRNA sequences. In addition, each microRNA precursor hairpin has the potential to produce two different mature microRNAs. We have investigated the evolutionary and functional relationships between the multiple produc ...
finding the genes that regulate development
... (2) The realisation that such genes and basic developmental events are extremely highly conserved through evolution. So observations made in one organism (e.g. Drosophila) have wider relevance to all animals (including humans). (3) The development of molecular techniques to manipulate developmental ...
... (2) The realisation that such genes and basic developmental events are extremely highly conserved through evolution. So observations made in one organism (e.g. Drosophila) have wider relevance to all animals (including humans). (3) The development of molecular techniques to manipulate developmental ...
Detecting a Transposon in Corn
... crossed into a corn strain to produce Ds insertions in genes. The Ac/Ds mutagenesis system also works well in a number of other plants including tobacco, tomato, and the model plant Arabidopsis thaliana. This laboratory investigates a bronze (bz) mutant of maize to analyze the molecular relationship ...
... crossed into a corn strain to produce Ds insertions in genes. The Ac/Ds mutagenesis system also works well in a number of other plants including tobacco, tomato, and the model plant Arabidopsis thaliana. This laboratory investigates a bronze (bz) mutant of maize to analyze the molecular relationship ...
Transcription in Eukaryotes
... Enhancers and silencers • Usually 700 to 1000 bp or more away from the start of transcription. • Increase or repress gene promoter activity either in all tissues or in a regulated manner. • Typically contain ~10 binding sites for several different transcription factors. • How can you tell an enhanc ...
... Enhancers and silencers • Usually 700 to 1000 bp or more away from the start of transcription. • Increase or repress gene promoter activity either in all tissues or in a regulated manner. • Typically contain ~10 binding sites for several different transcription factors. • How can you tell an enhanc ...
(CH11) Transcription In Eukaryotes (Slides)
... Enhancers and silencers • Usually 700 to 1000 bp or more away from the start of transcription. • Increase or repress gene promoter activity either in all tissues or in a regulated manner. • Typically contain ~10 binding sites for several different transcription factors. • How can you tell an enhanc ...
... Enhancers and silencers • Usually 700 to 1000 bp or more away from the start of transcription. • Increase or repress gene promoter activity either in all tissues or in a regulated manner. • Typically contain ~10 binding sites for several different transcription factors. • How can you tell an enhanc ...
Computational Biology
... (e.g. level of conservation of amino acids) of individual genes/proteins. Genetic distance is defined as # mismatches / # matches. Sequence conservation depends on physico-chemical properties of amino acids (and genome context such as G+C content). ...
... (e.g. level of conservation of amino acids) of individual genes/proteins. Genetic distance is defined as # mismatches / # matches. Sequence conservation depends on physico-chemical properties of amino acids (and genome context such as G+C content). ...
transcription factor
... Enhancers and silencers • Usually 700 to 1000 bp or more away from the start of transcription. • Increase or repress gene promoter activity either in all tissues or in a regulated manner. • Typically contain ~10 binding sites for several different transcription factors. • How can you tell an enhanc ...
... Enhancers and silencers • Usually 700 to 1000 bp or more away from the start of transcription. • Increase or repress gene promoter activity either in all tissues or in a regulated manner. • Typically contain ~10 binding sites for several different transcription factors. • How can you tell an enhanc ...
Annotation Practice Activity [Based on materials from the GEP
... The phase is the number of bases “left over” after the last full codon in the exon. Thus, to prevent a frame shift, the phases at the end of exon and the beginning of the next must add up to three. Sometimes there are multiple possible splice sites so you will need to determine the most likely corre ...
... The phase is the number of bases “left over” after the last full codon in the exon. Thus, to prevent a frame shift, the phases at the end of exon and the beginning of the next must add up to three. Sometimes there are multiple possible splice sites so you will need to determine the most likely corre ...
The DNA sequence of the fragment Hind.30, 378 bases lcng, fran
... The DNA sequence of the fragment Hind.30, 378 bases lcng, fran the beginning of gene 1 of T7 is presented. It contains the C promoter, two ill vitro transcriptianal terminator sites and a sequence of 171 bases which probably codes for the N terminus of the T7 RNA polymerase. The sequence also codes ...
... The DNA sequence of the fragment Hind.30, 378 bases lcng, fran the beginning of gene 1 of T7 is presented. It contains the C promoter, two ill vitro transcriptianal terminator sites and a sequence of 171 bases which probably codes for the N terminus of the T7 RNA polymerase. The sequence also codes ...
Patchy distribution of flexible genetic elements in bacterial
... differ, depending on the scale at which a study of interest is conducted. On the ecological scale, a population is defined to be a group of individuals of the same species within the same habitat at the same time that interact with one another (Lowe et al., 2004; Waples & Gaggiotti, 2006). On the ev ...
... differ, depending on the scale at which a study of interest is conducted. On the ecological scale, a population is defined to be a group of individuals of the same species within the same habitat at the same time that interact with one another (Lowe et al., 2004; Waples & Gaggiotti, 2006). On the ev ...
The complete nucleotide sequence of the chick a
... are similar but not identical. In a l l , 6 different actin iso-proteins have been identified in birds and mammals (3,1*,?). Four of these are a-actins or muscle actins obtained from 1* different muscle tissue types (skeletal striated, cardiac striated, vascular smooth, and enteric smooth). The a-ty ...
... are similar but not identical. In a l l , 6 different actin iso-proteins have been identified in birds and mammals (3,1*,?). Four of these are a-actins or muscle actins obtained from 1* different muscle tissue types (skeletal striated, cardiac striated, vascular smooth, and enteric smooth). The a-ty ...
Chapter 14: Gene Transcription and RNA Modification
... 14.4 RNA Modification Overview In eukaryotic organisms, transcription does not directly produce a functional mRNA. Instead, following transcription, the pre-mRNA molecule is modified by a number of different processes that can drastically alter the size and function of the pre-mRNA produced by the ...
... 14.4 RNA Modification Overview In eukaryotic organisms, transcription does not directly produce a functional mRNA. Instead, following transcription, the pre-mRNA molecule is modified by a number of different processes that can drastically alter the size and function of the pre-mRNA produced by the ...
Replicational and transcriptional selection on codon usage in
... the 59-dimensional space. The second axis accounted for only 6.1%, and no other axis accounted for more than 5%. Most of the variation in the second axis was because of a small number of outliers, which were annotated mostly as short, hypothetical proteins. It is probable that these hypothetical pro ...
... the 59-dimensional space. The second axis accounted for only 6.1%, and no other axis accounted for more than 5%. Most of the variation in the second axis was because of a small number of outliers, which were annotated mostly as short, hypothetical proteins. It is probable that these hypothetical pro ...
MCB 371/372 - Gogarten Lab | UConn
... 1) download Cn3D 4.2 from the CD-Tree release (http://www.ncbi.nlm.nih.gov/Structure/cdtree/cdtree.shtml) 2) Load the cd of interest into Cn3D 4.2 (find the cd on the web and click structure view to view it in cn3d 4.2 3) In the sequence window of cn3d 4.2, choose View/Export/PSSM – this will produc ...
... 1) download Cn3D 4.2 from the CD-Tree release (http://www.ncbi.nlm.nih.gov/Structure/cdtree/cdtree.shtml) 2) Load the cd of interest into Cn3D 4.2 (find the cd on the web and click structure view to view it in cn3d 4.2 3) In the sequence window of cn3d 4.2, choose View/Export/PSSM – this will produc ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.