Activation of Silent Genes by Transposons Tn5 and TnlO
... of the target region and their own transcripts are terminatedbeforereachingthenextdownstream gene. This suggests that polarity sites are common and areusually present within the untranslated region downstream of the element. Insertions activate the adjacent gene only if no polarity site is located b ...
... of the target region and their own transcripts are terminatedbeforereachingthenextdownstream gene. This suggests that polarity sites are common and areusually present within the untranslated region downstream of the element. Insertions activate the adjacent gene only if no polarity site is located b ...
The evolutionary causes and consequences of sex
... in effective population size (Ne), which introduces a trade-off. This may explain why the Y chromosome of flies and mammals contains only a handful of genes that are essential for male fertility; most genes of this type are located elsewhere in the genome. Nonetheless, it is evident from recent expe ...
... in effective population size (Ne), which introduces a trade-off. This may explain why the Y chromosome of flies and mammals contains only a handful of genes that are essential for male fertility; most genes of this type are located elsewhere in the genome. Nonetheless, it is evident from recent expe ...
Identification of large-scale human-specific copy number
... Number of changes detected by aCGH in primate genomes For each of the primate species investigated (PTR, PPA, GGO, PPY and MFA), DNA from ten unrelated females was pooled, labeled and hybridized together with the differently labeled human reference DNA pool, also consisting of ten unrelated (female) ...
... Number of changes detected by aCGH in primate genomes For each of the primate species investigated (PTR, PPA, GGO, PPY and MFA), DNA from ten unrelated females was pooled, labeled and hybridized together with the differently labeled human reference DNA pool, also consisting of ten unrelated (female) ...
Document
... A. Are the sequence counterparts co-linear with respect to human? If not, is their evidence of genomics rearrangements in this region? Which ...
... A. Are the sequence counterparts co-linear with respect to human? If not, is their evidence of genomics rearrangements in this region? Which ...
The vertebrate genome annotation (Vega) database
... Generating the database for the Vega website As mentioned in Ashurst et al. (2), the data released via the Vega website is produced by merging two in-house databases at the Sanger Institute: the pipeline database containing the genome assembly and alignments of features (mRNAs, proteins and ESTs, ge ...
... Generating the database for the Vega website As mentioned in Ashurst et al. (2), the data released via the Vega website is produced by merging two in-house databases at the Sanger Institute: the pipeline database containing the genome assembly and alignments of features (mRNAs, proteins and ESTs, ge ...
No Slide Title
... of their fragments, performed by the program FPC. FPC uses an algorithm to cluster clones into contigs based on their probability of coincidence score. For each contig, it builds a consensus band (CB) map which is similar to a restriction map; but it does not try to resolve all the errors. The CB ma ...
... of their fragments, performed by the program FPC. FPC uses an algorithm to cluster clones into contigs based on their probability of coincidence score. For each contig, it builds a consensus band (CB) map which is similar to a restriction map; but it does not try to resolve all the errors. The CB ma ...
Full-Text PDF
... multiplied, and it plays an important role in plant evolution [4]. Two forms of polyploidy are often considered: allopolyploidy, which originates from interspecies hybrids, and autopolyploidy, which originates from intraspecies genome duplication events. Polyploidy is particularly widespread in the ...
... multiplied, and it plays an important role in plant evolution [4]. Two forms of polyploidy are often considered: allopolyploidy, which originates from interspecies hybrids, and autopolyploidy, which originates from intraspecies genome duplication events. Polyploidy is particularly widespread in the ...
Document
... • Pre-defined six generic aspects for summarizing genes: – GP (Gene Product): describing the product (protein, rRNA, etc.) of the target gene; Ex. The eag gene encodes a polypeptide that shares sequence similarities with several different ionic channel proteins… – EL (Expression Location): describin ...
... • Pre-defined six generic aspects for summarizing genes: – GP (Gene Product): describing the product (protein, rRNA, etc.) of the target gene; Ex. The eag gene encodes a polypeptide that shares sequence similarities with several different ionic channel proteins… – EL (Expression Location): describin ...
Slide 1
... If the first T and the second –P were deleted, the relative position of T and –P would change. ...
... If the first T and the second –P were deleted, the relative position of T and –P would change. ...
Sex-linked dosage-sensitive modifiers as imprinting
... Many modifiers of variegating position-effects (Locke et al. 1988), as well as modifiers of other phenotypes in Drosophila (Rabinow and Birchler, 1989), are responsive to gene dosage. Locke et al. (1988) have described a number of modifying genes that affect white-mottled and yellow variegation. The ...
... Many modifiers of variegating position-effects (Locke et al. 1988), as well as modifiers of other phenotypes in Drosophila (Rabinow and Birchler, 1989), are responsive to gene dosage. Locke et al. (1988) have described a number of modifying genes that affect white-mottled and yellow variegation. The ...
fig. 1 - Utrecht University Repository
... on the bit-string marker. There is also a fixed per-gene probability of loss (l). De novo gene discovery and gene duplication do not happen as a result of replicating the genome for reproduction. However, gene duplications and gene discovery can both be the result of the simplified form of HGT in th ...
... on the bit-string marker. There is also a fixed per-gene probability of loss (l). De novo gene discovery and gene duplication do not happen as a result of replicating the genome for reproduction. However, gene duplications and gene discovery can both be the result of the simplified form of HGT in th ...
Studies of the Growth Hormone-Prolactin Gene Family and their
... frequent duplications are small and result in the formation of pseudogenes. From this perspective I will discuss the mechanisms of unequal crossing over, retrotransposition and chromosome segment duplication to give an image of the plasticity of genomes and the way in which these processes dynamical ...
... frequent duplications are small and result in the formation of pseudogenes. From this perspective I will discuss the mechanisms of unequal crossing over, retrotransposition and chromosome segment duplication to give an image of the plasticity of genomes and the way in which these processes dynamical ...
Molecular Cloning of engrafted: A Gene Involved in the
... Comparison of restriction digests and hybridization analyses indicated when the two separate walks overlapped. A total of 225 kb of DNA was cloned from the 48AB region (Figure 2) and 205 kb were found to be deleted by en sF31. It is notable that in the course of this work a number of different chrom ...
... Comparison of restriction digests and hybridization analyses indicated when the two separate walks overlapped. A total of 225 kb of DNA was cloned from the 48AB region (Figure 2) and 205 kb were found to be deleted by en sF31. It is notable that in the course of this work a number of different chrom ...
Studying copy number variations using a nanofluidic platform
... tcag.ca/variation). A recent paper demonstrated the presence of 525 novel insertion sequences across the genomes of eight unrelated individuals, which were not present in the human reference genome, and showed that many of these have different copy numbers (10). However, the current CNV analysis is m ...
... tcag.ca/variation). A recent paper demonstrated the presence of 525 novel insertion sequences across the genomes of eight unrelated individuals, which were not present in the human reference genome, and showed that many of these have different copy numbers (10). However, the current CNV analysis is m ...
Application of PCR-technique in biological labs
... Prokaryotes RT-PCR is very useful in the insertion of eukaryotic genes into prokaryotes. Most eukaryotic genes contain introns in the genome but not in the mature mRNA, the cDNA generated from a RT-PCR reaction is the DNA sequence which is directly translated into protein after transcription. When t ...
... Prokaryotes RT-PCR is very useful in the insertion of eukaryotic genes into prokaryotes. Most eukaryotic genes contain introns in the genome but not in the mature mRNA, the cDNA generated from a RT-PCR reaction is the DNA sequence which is directly translated into protein after transcription. When t ...
Genomic Gene Clustering Analysis of Pathways
... genes were found in uber-operons as well. Lathe et al. (2000) showed that uber-operons can be used to correctly predict function of ‘hypothetical’ proteins. Operons that have been detected in eukaryotes differ from those in prokaryotes in that eukaryotic polycistronic mRNAs are not translatable dire ...
... genes were found in uber-operons as well. Lathe et al. (2000) showed that uber-operons can be used to correctly predict function of ‘hypothetical’ proteins. Operons that have been detected in eukaryotes differ from those in prokaryotes in that eukaryotic polycistronic mRNAs are not translatable dire ...
Gene - Representing Genes
... different mutation from each parent, but they are in the same gene, then it will have no mutation-free copy of that gene and will be a phenotypic mutant. Thus, crossing two mutant lines to produce offspring with the two mutations in trans-position tests whether they are in the same gene. If, however ...
... different mutation from each parent, but they are in the same gene, then it will have no mutation-free copy of that gene and will be a phenotypic mutant. Thus, crossing two mutant lines to produce offspring with the two mutations in trans-position tests whether they are in the same gene. If, however ...
Dian Yang - A Critical Review of Gene Set Enrichment Analysis: Development and Improvement
... single gene analysis usually misses some important effects on pathways. Modest changes in all genes encoding members of a biological pathway may alter the pathway dramatically and might ev ...
... single gene analysis usually misses some important effects on pathways. Modest changes in all genes encoding members of a biological pathway may alter the pathway dramatically and might ev ...
Homogenisation in the ribosomal RNA genes of an Epichloe
... Finally, evidence is presented that suggests the rate of ...
... Finally, evidence is presented that suggests the rate of ...
Topic #2: Should adults seek genome editing as a treatment for their
... The genome in every cell in an individual’s body is essentially identical - with a few notable exceptions: for example, the reproductive cells and the mutations acquired by each cell in a person’s lifetime. With these caveats in mind, in theory, the DNA of virtually any cell can be analyzed to provi ...
... The genome in every cell in an individual’s body is essentially identical - with a few notable exceptions: for example, the reproductive cells and the mutations acquired by each cell in a person’s lifetime. With these caveats in mind, in theory, the DNA of virtually any cell can be analyzed to provi ...
Genome editing and CRISPR Aim - Personal Genetics Education
... The genome in every cell in an individual’s body is essentially identical - with a few notable exceptions: for example, the reproductive cells and the mutations acquired by each cell in a person’s lifetime. With these caveats in mind, in theory, the DNA of virtually any cell can be analyzed to provi ...
... The genome in every cell in an individual’s body is essentially identical - with a few notable exceptions: for example, the reproductive cells and the mutations acquired by each cell in a person’s lifetime. With these caveats in mind, in theory, the DNA of virtually any cell can be analyzed to provi ...
physical maps
... Ready access to identification of known human polymorphism Speeds mapping of new organisms by comparison ...
... Ready access to identification of known human polymorphism Speeds mapping of new organisms by comparison ...
File - Alexis Kezirian
... site with a loxP site, upstream of the Lnp gene. The other transgenic line used the same (inverted) Hoxd9lacZ transgene, inserted into the rel5 site with a loxP site, downstream of the ltga6 ex1-24 gene. b) Figure C compares the expression of the LacZ gene product in maternallyand paternally-inherit ...
... site with a loxP site, upstream of the Lnp gene. The other transgenic line used the same (inverted) Hoxd9lacZ transgene, inserted into the rel5 site with a loxP site, downstream of the ltga6 ex1-24 gene. b) Figure C compares the expression of the LacZ gene product in maternallyand paternally-inherit ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.