Specific function of a plastid sigma factor for ndhF
... SIG5 and SIG6 mRNAs. For SIG2 mRNA, the hybridization temperature was lowered to 48 C. Amplification products were separated by electrophoresis on agarose (1.2%) gels. For semi-quantitative amplification of ndhF and psbN mRNAs only 1 mg of DNase I-treated total RNA was used. After cDNA synthesis us ...
... SIG5 and SIG6 mRNAs. For SIG2 mRNA, the hybridization temperature was lowered to 48 C. Amplification products were separated by electrophoresis on agarose (1.2%) gels. For semi-quantitative amplification of ndhF and psbN mRNAs only 1 mg of DNase I-treated total RNA was used. After cDNA synthesis us ...
File - Alexis Kezirian
... site with a loxP site, upstream of the Lnp gene. The other transgenic line used the same (inverted) Hoxd9lacZ transgene, inserted into the rel5 site with a loxP site, downstream of the ltga6 ex1-24 gene. b) Figure C compares the expression of the LacZ gene product in maternallyand paternally-inherit ...
... site with a loxP site, upstream of the Lnp gene. The other transgenic line used the same (inverted) Hoxd9lacZ transgene, inserted into the rel5 site with a loxP site, downstream of the ltga6 ex1-24 gene. b) Figure C compares the expression of the LacZ gene product in maternallyand paternally-inherit ...
Identification of R-Gene Homologous DNA Fragments Genetically
... of these can be distinguished by the presence of an Nterminal nucleotide binding site and a C-terminal stretch of leucine-rich repeats. Although these gene products are structurally related, the DNA sequences are poorly conserved. Only parts of the nucleotide binding site share enough DNA identity t ...
... of these can be distinguished by the presence of an Nterminal nucleotide binding site and a C-terminal stretch of leucine-rich repeats. Although these gene products are structurally related, the DNA sequences are poorly conserved. Only parts of the nucleotide binding site share enough DNA identity t ...
The evolution of large DNA viruses: combining genomic information
... Box 1. The origin of DNA viruses Numerous theories exist to explain the origins of DNA viruses. Some propose that the ancestors of modern viruses appeared before cells [45,47]. For example, it has been suggested that ‘micelles’, which had the tendency to trap and protect nucleic acids, formed from p ...
... Box 1. The origin of DNA viruses Numerous theories exist to explain the origins of DNA viruses. Some propose that the ancestors of modern viruses appeared before cells [45,47]. For example, it has been suggested that ‘micelles’, which had the tendency to trap and protect nucleic acids, formed from p ...
scylla and charybde, homologues of the human apoptotic gene
... products represent the prototypes for a new family of proteins. These related genes code for pioneer proteins, and their deduced polypeptide sequences are most notable for excluding, rather than suggesting, protein function. Although the biochemical mechanism of the RTP801, Scylla and Charybde prote ...
... products represent the prototypes for a new family of proteins. These related genes code for pioneer proteins, and their deduced polypeptide sequences are most notable for excluding, rather than suggesting, protein function. Although the biochemical mechanism of the RTP801, Scylla and Charybde prote ...
Molecular Cloning of Streptococcus bovis Lactose
... The positions of the lac genes within the 7.8 kb HindIII fragment were determined by a combination of transposon mutagenesis and the deletion and subcloning of various restriction fragments into pUC 19, followed by determination of P-D-galactosidase, lactose permease and thiogalactoside transacetyla ...
... The positions of the lac genes within the 7.8 kb HindIII fragment were determined by a combination of transposon mutagenesis and the deletion and subcloning of various restriction fragments into pUC 19, followed by determination of P-D-galactosidase, lactose permease and thiogalactoside transacetyla ...
Genome Biology and Evolution
... Monophlebidae. Further analyses should be done to determine whether other Flavobacteria that have been previously obtained from insects of the family Coccidae and Lecanodiaspididae whose 16S rRNA sequences are phylogenetically related to Walczuchella monophlebidarum (Rosenblueth et al. 2012) could b ...
... Monophlebidae. Further analyses should be done to determine whether other Flavobacteria that have been previously obtained from insects of the family Coccidae and Lecanodiaspididae whose 16S rRNA sequences are phylogenetically related to Walczuchella monophlebidarum (Rosenblueth et al. 2012) could b ...
Large-Scale Variation Among Human and Great Ape Genomes
... comparative genomic hybridization (array CGH), measuring copy-number gains and losses among these species. Using an array of 2460 human bacterial artificial chromosomes (BACs) (12% of the genome), we identified a total of 63 sites of putative DNA copy-number variation between humans and the great ap ...
... comparative genomic hybridization (array CGH), measuring copy-number gains and losses among these species. Using an array of 2460 human bacterial artificial chromosomes (BACs) (12% of the genome), we identified a total of 63 sites of putative DNA copy-number variation between humans and the great ap ...
Localization of Three Genes in the Hook
... the conclusions from this work were preliminary. Finally, work in Drosophila identified several packaging motifs of the four chromosomes that were shared in all the nuclei examined, some of which were tissue-specific [23-25]. There was, however, a wide range of configurations for each chromosome suc ...
... the conclusions from this work were preliminary. Finally, work in Drosophila identified several packaging motifs of the four chromosomes that were shared in all the nuclei examined, some of which were tissue-specific [23-25]. There was, however, a wide range of configurations for each chromosome suc ...
Characterization of Two Rice MADS Box Genes That Control
... organ identity. We have characterized two flower-specific cDNAs from rice, designated OsMADS7 and OsMADS8. The cDNAs displayed the structure of a typical plant MADS box gene, which consists of the MADS domain, I region, K domain, and C-terminal region. These genes were classified as members of the A ...
... organ identity. We have characterized two flower-specific cDNAs from rice, designated OsMADS7 and OsMADS8. The cDNAs displayed the structure of a typical plant MADS box gene, which consists of the MADS domain, I region, K domain, and C-terminal region. These genes were classified as members of the A ...
Summary 121 Summary The Hox genes form a subset of the
... homeobox encodes a DNA binding motif, called the homeodomain. In most animal species the Hox genes are organised in one or more clusters. The number of genes present in a cluster varies between animal species; the number of clusters in each species also varies. The Hox clusters are thought to have a ...
... homeobox encodes a DNA binding motif, called the homeodomain. In most animal species the Hox genes are organised in one or more clusters. The number of genes present in a cluster varies between animal species; the number of clusters in each species also varies. The Hox clusters are thought to have a ...
CHEM642-07 Powerpoint
... the efficiency of each step and on the rates of degradation of the RNA and protein molecules. (A) In eucaryotic cells the RNA molecule produced by transcription alone (sometimes referred to as the primary transcript) would contain both coding (exon) and noncoding (intron) sequences. Before it can be ...
... the efficiency of each step and on the rates of degradation of the RNA and protein molecules. (A) In eucaryotic cells the RNA molecule produced by transcription alone (sometimes referred to as the primary transcript) would contain both coding (exon) and noncoding (intron) sequences. Before it can be ...
Using real time RT-PCR analysis to determine multiple gene
... regulating gonad development for the limited number of genes that have been identified. As the list of genes increases, studying their interactions will become even more daunting. One approach to investigate gene interactions in genital ridge development and/or gonadal sex differentiation is to comp ...
... regulating gonad development for the limited number of genes that have been identified. As the list of genes increases, studying their interactions will become even more daunting. One approach to investigate gene interactions in genital ridge development and/or gonadal sex differentiation is to comp ...
Performance Comparison of Two Gene Set Analysis Methods for
... second-best one [4]. We compared the distribution of gene scores that were calculated based on the best and the second-best p-values using a high-volume scatter plot that represented the local density of points by a false color representation (Fig. 1). One may notice the densely populated points alo ...
... second-best one [4]. We compared the distribution of gene scores that were calculated based on the best and the second-best p-values using a high-volume scatter plot that represented the local density of points by a false color representation (Fig. 1). One may notice the densely populated points alo ...
Using a Single Nucleotide Polymorphism to Predict Bitter
... Some sequences are found at only one site -- a single locus - in the human genome. For many tandem repeats, the number of repeated units vary between individuals. Such loci are termed VNTRs. One VNTR in humans is a 17 bp sequence of DNA repeated between 70 and 450 times in the genome. The total numb ...
... Some sequences are found at only one site -- a single locus - in the human genome. For many tandem repeats, the number of repeated units vary between individuals. Such loci are termed VNTRs. One VNTR in humans is a 17 bp sequence of DNA repeated between 70 and 450 times in the genome. The total numb ...
comparing quantitative trait loci and gene expression data
... of piecewise regression. However at the edge of chromosomes and some middle places where possibly near to the cutting points of the “smoothing windows”, we found that Expressionview gives apparent poor estimations. In those cases we use polynomial regression to estimate physical distance from cM by ...
... of piecewise regression. However at the edge of chromosomes and some middle places where possibly near to the cutting points of the “smoothing windows”, we found that Expressionview gives apparent poor estimations. In those cases we use polynomial regression to estimate physical distance from cM by ...
New techniques in plant biotechnology
... genetically modified rootstock, gene silencing by DNA methylation, the use of oligonucleotides, and specific mutagenesis with homologous recombination. These techniques were chosen as they are either in the early stages of commercial application or give insight into the problem at stake. For some of ...
... genetically modified rootstock, gene silencing by DNA methylation, the use of oligonucleotides, and specific mutagenesis with homologous recombination. These techniques were chosen as they are either in the early stages of commercial application or give insight into the problem at stake. For some of ...
- Wiley Online Library
... right time, and with the proper rate. Metazoan developmental genes often possess long stretches of DNA flanking their coding sequences and/or large introns which contain elements that influence gene expression. Most of these regulatory elements are relatively small and can be studied in isolation. F ...
... right time, and with the proper rate. Metazoan developmental genes often possess long stretches of DNA flanking their coding sequences and/or large introns which contain elements that influence gene expression. Most of these regulatory elements are relatively small and can be studied in isolation. F ...
Homeotic selector genes
... – since there are multiple copies of each HOM-C gene, it is difficult to obtain perfect homeotic transformations • important concept: functional redundancy • this means that the if one gene is knocked out, the remaining paralogous genes can partially compensate – HOX-C and HOM-C genes bind to the sa ...
... – since there are multiple copies of each HOM-C gene, it is difficult to obtain perfect homeotic transformations • important concept: functional redundancy • this means that the if one gene is knocked out, the remaining paralogous genes can partially compensate – HOX-C and HOM-C genes bind to the sa ...
The Maize Genome Poster
... Corn, also known as maize (from the Spanish maíz), was first domesticated nearly 10,000 years ago from teosinte, a wild grass that looked quite different from our modern crop. Teosinte grew in Mexico and Central America as a bushy plant with many spikes, the precursor to our familiar ear of corn. Th ...
... Corn, also known as maize (from the Spanish maíz), was first domesticated nearly 10,000 years ago from teosinte, a wild grass that looked quite different from our modern crop. Teosinte grew in Mexico and Central America as a bushy plant with many spikes, the precursor to our familiar ear of corn. Th ...
Sequencing Crop Genomes - Tropical Life Sciences Research
... O. sativa were distributed among the groups from 11 different countries (China, Japan, India, United States of America, United Kingdom, Taiwan, Korea, Thailand, France, Brazil, and Canada) (Eckardt 2000). Some private firms also contributed to the rice genome sequencing. In 2000, Monsanto completed ...
... O. sativa were distributed among the groups from 11 different countries (China, Japan, India, United States of America, United Kingdom, Taiwan, Korea, Thailand, France, Brazil, and Canada) (Eckardt 2000). Some private firms also contributed to the rice genome sequencing. In 2000, Monsanto completed ...
A simple set of rules for primer sequence design is as follows
... The optimum length of a primer depends upon its (A+T) content, and the Tm. Apart from the Tm, a prime consideration is that the primers should be complex enough so that the likelihood of annealing to sequences other than the chosen target is very low. For example, there is a ¼ chance of finding an A ...
... The optimum length of a primer depends upon its (A+T) content, and the Tm. Apart from the Tm, a prime consideration is that the primers should be complex enough so that the likelihood of annealing to sequences other than the chosen target is very low. For example, there is a ¼ chance of finding an A ...
Goldmine: Integrating information to place sets of genomic ranges
... By default, Goldmine will ensure that the latest versions of reference tables from the UCSC Genome Browser are obtained. This is accomplished by comparing the date of the version in the cache to the date of the version on UCSC's server, and if UCSC's version is newer, the new version will be downloa ...
... By default, Goldmine will ensure that the latest versions of reference tables from the UCSC Genome Browser are obtained. This is accomplished by comparing the date of the version in the cache to the date of the version on UCSC's server, and if UCSC's version is newer, the new version will be downloa ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.