Drosophila Muller F Elements Maintain a Distinct Set of Genomic
... comparative analyses of the repeat and gene characteristics of the F element in four Drosophila species. The Drosophila 12 Genomes Consortium (Drosophila 12 Genomes Consortium et al. 2007) and the modENCODE project (Kharchenko et al. 2011) have produced a large collection of genomic datasets for D. ...
... comparative analyses of the repeat and gene characteristics of the F element in four Drosophila species. The Drosophila 12 Genomes Consortium (Drosophila 12 Genomes Consortium et al. 2007) and the modENCODE project (Kharchenko et al. 2011) have produced a large collection of genomic datasets for D. ...
Concept_Paper
... Nobel-laureated codiscovery of catalytic RNA (ribozymes) [19]; Discovery of the function of histone acetylation [20]. Advanced molecular and genetic tools developed in Tetrahymena have maintained this organism at the forefront of fundamental research. This is particularly the case in areas that ...
... Nobel-laureated codiscovery of catalytic RNA (ribozymes) [19]; Discovery of the function of histone acetylation [20]. Advanced molecular and genetic tools developed in Tetrahymena have maintained this organism at the forefront of fundamental research. This is particularly the case in areas that ...
Tracing the origin of our species through palaeogenomics
... this case the one of present-day humans, since a de novo assemblage of an ancient genome based on these short molecules is not possible. The sequences may contain mistakes due to the damages that occur in DNA over time, therefore a higher coverage is required. Ideally each base should be sequenced a ...
... this case the one of present-day humans, since a de novo assemblage of an ancient genome based on these short molecules is not possible. The sequences may contain mistakes due to the damages that occur in DNA over time, therefore a higher coverage is required. Ideally each base should be sequenced a ...
Two genes from Bacillus subtilis under the sole control
... following section, DNA sequence analysis found a large ORF encoded by this newly isolated chromosomal region. T o locate the promoter activity or activities for this frame, we made a transcriptional fusion to the lacZ reporter gene in the single-copy vector pDG268 (Antoniewski et al., 1990). As show ...
... following section, DNA sequence analysis found a large ORF encoded by this newly isolated chromosomal region. T o locate the promoter activity or activities for this frame, we made a transcriptional fusion to the lacZ reporter gene in the single-copy vector pDG268 (Antoniewski et al., 1990). As show ...
UNIFR Rusconi 2002
... Basic understanding of 'genes and genomes': what is a gene, how many genes, molecular biology dogma genetic diseases, environmental factors, Essential concepts on 'molecular medicine' applications and problems, ageing as the major disease Genomics and other 'omics' essential technologies Application ...
... Basic understanding of 'genes and genomes': what is a gene, how many genes, molecular biology dogma genetic diseases, environmental factors, Essential concepts on 'molecular medicine' applications and problems, ageing as the major disease Genomics and other 'omics' essential technologies Application ...
Cells: A Multiple Time Point Analysis Chronic Lymphocytic
... (16, 17, 22–24), was used as a template (100 ng) for PCR in a volume of 50 ml containing 200 mM of each dNTP, 2.5 U of Taq polymerase (PerkinElmer Cetus Corp., Norwalk, CT), and 10 pmol of each oligonucleotide primer. Six individual PCR amplifications were performed for the H chain. Each reaction in ...
... (16, 17, 22–24), was used as a template (100 ng) for PCR in a volume of 50 ml containing 200 mM of each dNTP, 2.5 U of Taq polymerase (PerkinElmer Cetus Corp., Norwalk, CT), and 10 pmol of each oligonucleotide primer. Six individual PCR amplifications were performed for the H chain. Each reaction in ...
Transformation as a Tool for Genetic Analysis in Populus
... loads (Bradshaw and Strauss 2001; Bradshaw et al., 2001). Inbreeding to reveal recessive mutations is therefore poorly tolerated, and the expression of this load among progeny would make it difficult to distinguish the effects of specific gene lesions from the large number of additional loci whose m ...
... loads (Bradshaw and Strauss 2001; Bradshaw et al., 2001). Inbreeding to reveal recessive mutations is therefore poorly tolerated, and the expression of this load among progeny would make it difficult to distinguish the effects of specific gene lesions from the large number of additional loci whose m ...
Conservation and Coevolution in the Scale
... Oltvai 2004). Existing highly connected nodes (i.e., genes or proteins) are more likely, simply by virtue of their large number of connections, to be linked to nodes that are duplicated. Because the duplicated nodes are expected to maintain the same links as the ancestral singleton, the connectivity ...
... Oltvai 2004). Existing highly connected nodes (i.e., genes or proteins) are more likely, simply by virtue of their large number of connections, to be linked to nodes that are duplicated. Because the duplicated nodes are expected to maintain the same links as the ancestral singleton, the connectivity ...
What Do You Mean, “Epigenetic”?
... 2010). Those in the field of genetics concerned with DNA methylation, chromatin activity states, chromosomal imprinting, centromere function, etc., predominantly use Holliday’s notion of epigenetics. They are interested in how expression patterns persist across different cells (mitosis) and generatio ...
... 2010). Those in the field of genetics concerned with DNA methylation, chromatin activity states, chromosomal imprinting, centromere function, etc., predominantly use Holliday’s notion of epigenetics. They are interested in how expression patterns persist across different cells (mitosis) and generatio ...
AllBio_DJK
... Enriched signals at the QTL position • 16 differentially expressed probes in 1Mb region around QTL • QTL acting at chromatin or methylation level? • PH simply one of the downstream effects. ...
... Enriched signals at the QTL position • 16 differentially expressed probes in 1Mb region around QTL • QTL acting at chromatin or methylation level? • PH simply one of the downstream effects. ...
Shedding Genomic Ballast: Extensive Parallel Loss of Ancestral
... in which each of 3507 families present in at least two of the genomes was treated as a cladistic character (scored ‘‘present’’ or ‘‘absent’’). Three hundred seventy-one of these families were present in all genomes analyzed, 187 other families were parsimony-uninformative, and 2949 were parsimony-in ...
... in which each of 3507 families present in at least two of the genomes was treated as a cladistic character (scored ‘‘present’’ or ‘‘absent’’). Three hundred seventy-one of these families were present in all genomes analyzed, 187 other families were parsimony-uninformative, and 2949 were parsimony-in ...
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
... elements, five additional LTRs and six tRNA genes. Such DNA duplications form large regions of partial gene synteny between these two chromosomes. An example is a section from nucleotide 834,000 to 860,000 on chromosome XVI and from 762,422 to 777,500 on chromosome VII. In the chromosome XVI interva ...
... elements, five additional LTRs and six tRNA genes. Such DNA duplications form large regions of partial gene synteny between these two chromosomes. An example is a section from nucleotide 834,000 to 860,000 on chromosome XVI and from 762,422 to 777,500 on chromosome VII. In the chromosome XVI interva ...
Evolution of genes, evolution of species: the case of aminoacyl
... 1993). Conversely, it is only recently that the archaebacterial LysRS’s from Methanococcus maripaludis, Methanobacterium thermoautotrophicum, and Methanococcus jannaschii (but not that from Sulfolobus solfataricus) and the LysRS from the spirochete Borrelia burgdorferi have been shown to be radicall ...
... 1993). Conversely, it is only recently that the archaebacterial LysRS’s from Methanococcus maripaludis, Methanobacterium thermoautotrophicum, and Methanococcus jannaschii (but not that from Sulfolobus solfataricus) and the LysRS from the spirochete Borrelia burgdorferi have been shown to be radicall ...
Identification and characterization of an early gene in the Lymantria
... position 258 and ends at position 831. This gene encodes a 191 amino acid protein with a predicted M r of 22000. A region upstream of G22 is approximately 83% GC-rich (residues 70-202). Closer examination of the nucleotide sequence reveals that there are two types of identical direct repeats present ...
... position 258 and ends at position 831. This gene encodes a 191 amino acid protein with a predicted M r of 22000. A region upstream of G22 is approximately 83% GC-rich (residues 70-202). Closer examination of the nucleotide sequence reveals that there are two types of identical direct repeats present ...
Comprehensive Analysis of Amino Acid and Nucleotide
... mapping their positions on the chromosomes using BLAST (1) and related programs to search against protein databases(2-6). Here, we define pseudogenes as disabled copies of genes that do not produce a functional, full-length copy of a protein (7). Operationally, these are identified as regions of the ...
... mapping their positions on the chromosomes using BLAST (1) and related programs to search against protein databases(2-6). Here, we define pseudogenes as disabled copies of genes that do not produce a functional, full-length copy of a protein (7). Operationally, these are identified as regions of the ...
Genes for Cognitive Function: Developments on the X
... Developments in human genome research enabled the first steps toward a molecular understanding of cognitive function. That there are numerous genes on the X chromosome affecting intelligence at the lower end of the cognitive range is no longer in doubt. Naturally occurring mutations have so far led ...
... Developments in human genome research enabled the first steps toward a molecular understanding of cognitive function. That there are numerous genes on the X chromosome affecting intelligence at the lower end of the cognitive range is no longer in doubt. Naturally occurring mutations have so far led ...
DROSOPHILA MELANOGASTER - THE MODEL ORGANISM OF
... lines and thus regulatory sequences that give gene expression in many different tissues have been identified. Building on this approach, a further powerful P element based system (the GAL4-UAS system -see Figure 2) has been developed that allows patterns of enhancer-driven expression to be converted ...
... lines and thus regulatory sequences that give gene expression in many different tissues have been identified. Building on this approach, a further powerful P element based system (the GAL4-UAS system -see Figure 2) has been developed that allows patterns of enhancer-driven expression to be converted ...
the human y chromosome, in the light of evolution
... genes do not tend to jointly occupy particular chromo- ...
... genes do not tend to jointly occupy particular chromo- ...
Characterization of the trans-spliced transcriptome and polycistronic
... Schistosoma mansoni and herein we provide evidence that this type of gene expression is also employed by cestodes. Based on transcriptome data, we found around 10% of E. multilocularis genes processed involving trans-splicing. We also found that around 300 genes form part of a PTU. The majority of E ...
... Schistosoma mansoni and herein we provide evidence that this type of gene expression is also employed by cestodes. Based on transcriptome data, we found around 10% of E. multilocularis genes processed involving trans-splicing. We also found that around 300 genes form part of a PTU. The majority of E ...
X inactivation Xplained
... Silencing of one X chromosome by Xist. (a) The two X chromosome territories are depicted. Xist RNA produced from the Xic locus accumulates on the future Xi. This leads to the formation of a nuclear compartment that is devoid of the transcription machinery, such as RNA polymerase II (Pol II). Polycom ...
... Silencing of one X chromosome by Xist. (a) The two X chromosome territories are depicted. Xist RNA produced from the Xic locus accumulates on the future Xi. This leads to the formation of a nuclear compartment that is devoid of the transcription machinery, such as RNA polymerase II (Pol II). Polycom ...
Chapter 10 - McGraw Hill Higher Education
... The mice had their immune systems disabled The “healthy” gene was thus introduced into lung cells And the mice were successfully cured! Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... The mice had their immune systems disabled The “healthy” gene was thus introduced into lung cells And the mice were successfully cured! Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
Comparison between Human and Mouse genomes
... complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with signi ...
... complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with signi ...
Molecular Cloning and Nucleotide Sequence of the 3
... clearly read from one end to the other. However, if there were two proximal CluI sites or an EcoRI or BglII site which could not be detected by mapping with restriction endonucleases, they would be missed. To test this possibility we cleaved pYKL45 with one of ClaI, EcoRI and BglII completely, recir ...
... clearly read from one end to the other. However, if there were two proximal CluI sites or an EcoRI or BglII site which could not be detected by mapping with restriction endonucleases, they would be missed. To test this possibility we cleaved pYKL45 with one of ClaI, EcoRI and BglII completely, recir ...
The Functions of Introns: From Junk DNA to Designed DNA
... Variations in intron removal splicing patterns can be used to create two or more distinct mRNAs which code for different polypeptides. This allows one mRNA code to be used to produce a variety of polypeptides.33 Splicing variations are controlled by regulator proteins designated as SR proteins. SR p ...
... Variations in intron removal splicing patterns can be used to create two or more distinct mRNAs which code for different polypeptides. This allows one mRNA code to be used to produce a variety of polypeptides.33 Splicing variations are controlled by regulator proteins designated as SR proteins. SR p ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.