Analysis and nucleotide sequence of an origin of DNA replication in
... to the vector. A deletion analysis of the shuttle plasmid indicates that 2.2 kb is necessary for autonomous replication and stable maintenance in A. calcoaceticus. No rearrangements of the DNA or loss of plasmids are found in that organism, even in the absence of selective pressure, when this sequen ...
... to the vector. A deletion analysis of the shuttle plasmid indicates that 2.2 kb is necessary for autonomous replication and stable maintenance in A. calcoaceticus. No rearrangements of the DNA or loss of plasmids are found in that organism, even in the absence of selective pressure, when this sequen ...
Chapter 3 Clustering Microarray Data
... to uncover by giving equal weight to all the genes. Their procedure, Clustering Objects on Subsets of Attributes (COSA) computes distances between samples, giving the expression levels gene- and sample-specific weights. These distances are then passed to a distance-based clustering algorithm, such a ...
... to uncover by giving equal weight to all the genes. Their procedure, Clustering Objects on Subsets of Attributes (COSA) computes distances between samples, giving the expression levels gene- and sample-specific weights. These distances are then passed to a distance-based clustering algorithm, such a ...
Reconstruction of a Functional Human Gene Network, with an
... their functional interactions. By following up the top five genes per artificial locus, we were able to detect at least one known disease gene in 54% of the loci studied, representing a 2.8-fold increase over random selection. This suggests that our method can significantly reduce the cost and effor ...
... their functional interactions. By following up the top five genes per artificial locus, we were able to detect at least one known disease gene in 54% of the loci studied, representing a 2.8-fold increase over random selection. This suggests that our method can significantly reduce the cost and effor ...
Slide 1
... Use of enzymes and other products as tools for molecular biology techniques specially thermophylic products from thermophyl phages ...
... Use of enzymes and other products as tools for molecular biology techniques specially thermophylic products from thermophyl phages ...
Promega Notes: T4 RNA Ligase: A Molecular Tool for RNA and DNA
... to proceed was n=8 and that the reaction peaked at n=10-16 (8). The reaction then decreased as the chain length increased up to n=100 (8). One unit is defined as the amount of enzyme that catalyzes transformation of one nanomole of 5´-[32 P]poly(A) 14-20 into a phosphatase-resistant form in 30 minut ...
... to proceed was n=8 and that the reaction peaked at n=10-16 (8). The reaction then decreased as the chain length increased up to n=100 (8). One unit is defined as the amount of enzyme that catalyzes transformation of one nanomole of 5´-[32 P]poly(A) 14-20 into a phosphatase-resistant form in 30 minut ...
Chromocurvus halotolerans gen. nov., sp. nov., a
... medium A amended with the carbon sources glutamate, pyruvate, acetate, lactate, and malate (1 g/l each), and with vitamin solutions (Yurkov 2006) from which one of the following was removed for each treatment: biotin, B12, nicotinic acid, or thiamine. ...
... medium A amended with the carbon sources glutamate, pyruvate, acetate, lactate, and malate (1 g/l each), and with vitamin solutions (Yurkov 2006) from which one of the following was removed for each treatment: biotin, B12, nicotinic acid, or thiamine. ...
______________________________________________________________________ Análisis de la composición del regulón LexA en el dominio Resumen
... Previous studies carried out in our laboratory have analyzed the content of the LexA regulon of Gamma proteobacterias (Erill et al., 2003). Following the same line, the present work tries to extend the description of this regulon to other families of the Bacteria Domain: Geobacter sulfurreducens, re ...
... Previous studies carried out in our laboratory have analyzed the content of the LexA regulon of Gamma proteobacterias (Erill et al., 2003). Following the same line, the present work tries to extend the description of this regulon to other families of the Bacteria Domain: Geobacter sulfurreducens, re ...
PraderWilli syndrome resulting from an unbalanced translocation
... illustrate the tendency toward expanded phenotypes in these cases (21, 22). To our knowledge, only one instance of an unbalanced translocation (5;15) has been reported where the authors describe a PWS-like phenotype (23). This patient had severe mental retardation, a seizure disorder and hypotonia, ...
... illustrate the tendency toward expanded phenotypes in these cases (21, 22). To our knowledge, only one instance of an unbalanced translocation (5;15) has been reported where the authors describe a PWS-like phenotype (23). This patient had severe mental retardation, a seizure disorder and hypotonia, ...
illustra bacteria genomicPrep Mini Spin Kit
... Spin Kit performs effectively in quantitative real-time PCR. The performance of gDNA purified using the illustra bacteria genomicPrep Mini Spin Kit was compared to gDNA purified using the QIAamp DNA Mini Kit by performing quantitative real-time PCR analysis. No inhibitory effects were seen in either ...
... Spin Kit performs effectively in quantitative real-time PCR. The performance of gDNA purified using the illustra bacteria genomicPrep Mini Spin Kit was compared to gDNA purified using the QIAamp DNA Mini Kit by performing quantitative real-time PCR analysis. No inhibitory effects were seen in either ...
Nitrifyers in constructed wetlands treating landfill leachates Carina Sundberg
... efficiency but add little to an increased understanding of theorganisms performing the treatment or the spatial distribution of their activities and treatment processes. . In this thesis we investigated seasonal and annual changes in potential nitrification and denitrification, and in the correspond ...
... efficiency but add little to an increased understanding of theorganisms performing the treatment or the spatial distribution of their activities and treatment processes. . In this thesis we investigated seasonal and annual changes in potential nitrification and denitrification, and in the correspond ...
CHAPTER 15 THE CHROMOSOMAL BASIS OF INHERITANCE
... Whether an embryo develops into a male or female depends upon the presence of a Y chromosome. • A British research team has identified a gene, SRY (sex-determining region of Y), on the Y chromosome that is responsible for triggering the complex series of events that lead to normal testicular develop ...
... Whether an embryo develops into a male or female depends upon the presence of a Y chromosome. • A British research team has identified a gene, SRY (sex-determining region of Y), on the Y chromosome that is responsible for triggering the complex series of events that lead to normal testicular develop ...
Gene conversion rapidly generates major histocompatibility complex
... absolute estimates from data across populations need to be interpreted with caution. We developed a macro to quantify the level of shared polymorphism across unrelated haplotypes (hereafter ‘convergence’), using Minitab version 12.1 (available from the authors on request). Each haplotype (the ‘deriv ...
... absolute estimates from data across populations need to be interpreted with caution. We developed a macro to quantify the level of shared polymorphism across unrelated haplotypes (hereafter ‘convergence’), using Minitab version 12.1 (available from the authors on request). Each haplotype (the ‘deriv ...
how to analyze a splicing mutation - Stamm revision
... always be available. The majority of samples for clinical diagnostics are nearly always leukocytes from which, usually only the DNA is extracted. Extracted RNA is a relatively simple procedure, however, it is important to remember that the gene of interest may not be expressed in this tissue. Moreov ...
... always be available. The majority of samples for clinical diagnostics are nearly always leukocytes from which, usually only the DNA is extracted. Extracted RNA is a relatively simple procedure, however, it is important to remember that the gene of interest may not be expressed in this tissue. Moreov ...
Phenotypic and Genotypic Characterization of Rhizobium species
... These Isolates were studied for different salt concentration range from 5- 9.0%, and culture shows less growth as the concentration of salt increased .All isolates grow at pH 6.0 to 11.0 but none of the isolates grow at ph 12.0. The optimum physical parameters for growth of fast growing rhizobia wer ...
... These Isolates were studied for different salt concentration range from 5- 9.0%, and culture shows less growth as the concentration of salt increased .All isolates grow at pH 6.0 to 11.0 but none of the isolates grow at ph 12.0. The optimum physical parameters for growth of fast growing rhizobia wer ...
View/print full test page
... gene testing is desired for the patient or family members of an individual with a known mutation, that must be ordered separately. ...
... gene testing is desired for the patient or family members of an individual with a known mutation, that must be ordered separately. ...
NCBI Molecular Biology Resources
... XM records are models based only on genomic sequence, and are subject to revision or removal with each new build of that genome. BLAST the XM against the RefSeq database to look for a replacement: Query= gi|20850420|ref|XM_124429.1| Mus musculus expressed sequence AA553001 (AA553001), mRNA gi|19527 ...
... XM records are models based only on genomic sequence, and are subject to revision or removal with each new build of that genome. BLAST the XM against the RefSeq database to look for a replacement: Query= gi|20850420|ref|XM_124429.1| Mus musculus expressed sequence AA553001 (AA553001), mRNA gi|19527 ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... Our system works by replacing the standard file icons with automatically created icons that reflect the contents of the files in a principled way. We call such icons INTELLIGENT ICONS. While there is little utility in examining an individual icon, examining groups of them allows us to take advantage ...
... Our system works by replacing the standard file icons with automatically created icons that reflect the contents of the files in a principled way. We call such icons INTELLIGENT ICONS. While there is little utility in examining an individual icon, examining groups of them allows us to take advantage ...
From bedside to bench: how to analyze a splicing
... analysis of the affected tissue through a reverse transcriptase reaction followed by PCR using primers that amplify, preferably from exons as far away from the mutation location as possible. Though apparently straightforward, this approach carries problems. Firstly, the patient or the appropriate ti ...
... analysis of the affected tissue through a reverse transcriptase reaction followed by PCR using primers that amplify, preferably from exons as far away from the mutation location as possible. Though apparently straightforward, this approach carries problems. Firstly, the patient or the appropriate ti ...
B.Sc. (H) Microbiology THREE-YEAR FULL-TIME PROGRAMME Choice based credit system (Six-Semester Course)
... facilitate seamless mobility of students across various universities based on the credits. This credit based semester system will provide flexibility in designing curriculum and assigning credits based on the course contents and number of hours of teaching. In this system students have the option to ...
... facilitate seamless mobility of students across various universities based on the credits. This credit based semester system will provide flexibility in designing curriculum and assigning credits based on the course contents and number of hours of teaching. In this system students have the option to ...
Comparative Analysis of Structural Diversity and
... clear genes. Pearson’s correlation test was used to determine if there is any significant correlation between Ks and GC3s or Ks and ENC. The statistically significant level was set at 95%. The statistical analyses were conducted using the program R (http://www.r-project.org/). Results and Discussion ...
... clear genes. Pearson’s correlation test was used to determine if there is any significant correlation between Ks and GC3s or Ks and ENC. The statistically significant level was set at 95%. The statistical analyses were conducted using the program R (http://www.r-project.org/). Results and Discussion ...
RECOMBINANT DNA TECHNOLOGY AND BIOTECHNOLOGY
... the tracking dyes. Bromophenol blue and xylene cyanol dyes are used usually and they migrate through agarose gels at roughly the same rate as double-stranded DNA fragments of 300 and 4000 bp, respectively. When adequate migration has occured, DNA fragments are visualized by staining with ethidium br ...
... the tracking dyes. Bromophenol blue and xylene cyanol dyes are used usually and they migrate through agarose gels at roughly the same rate as double-stranded DNA fragments of 300 and 4000 bp, respectively. When adequate migration has occured, DNA fragments are visualized by staining with ethidium br ...
Transcription
... • The 5’ cap is a guanine nucleotide that has been methylated (7methyl guanine, m7G) and attached by a 5’5’ linkage to the first nucleotide of the transcript. There are 3 phosphate groups between the two nucleotides. • The 3’ end of newly transcribed RNA is protected by adding 100200 adenine nucleo ...
... • The 5’ cap is a guanine nucleotide that has been methylated (7methyl guanine, m7G) and attached by a 5’5’ linkage to the first nucleotide of the transcript. There are 3 phosphate groups between the two nucleotides. • The 3’ end of newly transcribed RNA is protected by adding 100200 adenine nucleo ...
Spectrum of Mutations in MMAB Identified by
... of L-methylmalonyl-CoA to succinyl-CoA. Somatic cells studies have been used to evaluate patient samples for cobalamin related disorders. Due to high basal levels of propionate incorporation, some patients with mild MMA biochemical phenotypes cannot be diagnosed by complementation analysis. A high r ...
... of L-methylmalonyl-CoA to succinyl-CoA. Somatic cells studies have been used to evaluate patient samples for cobalamin related disorders. Due to high basal levels of propionate incorporation, some patients with mild MMA biochemical phenotypes cannot be diagnosed by complementation analysis. A high r ...
univERsity oF copEnhAGEn
... biochemical parameters, that characterize the cells within a bacterial culture. In contrast, the genotype refer to genetical (DNA- or RNA-related) parameters such as genomic DNA structure, occurrence of plasmid DNA, sequence of ribosomal DNA or RNA, etc., although the latter may specifically be refe ...
... biochemical parameters, that characterize the cells within a bacterial culture. In contrast, the genotype refer to genetical (DNA- or RNA-related) parameters such as genomic DNA structure, occurrence of plasmid DNA, sequence of ribosomal DNA or RNA, etc., although the latter may specifically be refe ...
DNA Methylation Analysis
... bead-bound probes are used to detect the presence of T or C by hybridization followed by single-base extention with a labeled nucleotide. ...
... bead-bound probes are used to detect the presence of T or C by hybridization followed by single-base extention with a labeled nucleotide. ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.