Distribution and molecular characterization of
... gene cluster, which contains tetR and tetA, was cloned from one of the tetracycline-resistant strains, HLHK5. While the flanking regions and 30 end of the tetA of HLHK5 were identical to the corresponding regions of a tetC island in Chlamydia suis, the tetA gene was almost identical to that of trans ...
... gene cluster, which contains tetR and tetA, was cloned from one of the tetracycline-resistant strains, HLHK5. While the flanking regions and 30 end of the tetA of HLHK5 were identical to the corresponding regions of a tetC island in Chlamydia suis, the tetA gene was almost identical to that of trans ...
Analysis of single nucleotide polymorphisms in human
... The sequence variations in many important human genes have been extensively studied. In these studies, it was found that approximately half of the coding SNPs resulted in an amino acid change in the protein sequence [4-6]. Ng and Henikoff have predicted the effect of SNPs on protein function based o ...
... The sequence variations in many important human genes have been extensively studied. In these studies, it was found that approximately half of the coding SNPs resulted in an amino acid change in the protein sequence [4-6]. Ng and Henikoff have predicted the effect of SNPs on protein function based o ...
Murad Mohammed Hamed Shnaewer
... the phage life cycle after host infection. PPUDV showed a latent period of about 36 h, burst period of 70 h and a burst size of about 600 Plaque Forming Units (PFU) per infected cell. PPURV phage showed latent period of about 24 h, burst period of 47 h and a burst size of about 700 PFU per infected ...
... the phage life cycle after host infection. PPUDV showed a latent period of about 36 h, burst period of 70 h and a burst size of about 600 Plaque Forming Units (PFU) per infected cell. PPURV phage showed latent period of about 24 h, burst period of 47 h and a burst size of about 700 PFU per infected ...
A novel locus of Yersinia enterocolitica serotype O:3 involved in
... mutants. To test this, rough derivatives of previously constructed trans-complementation strains were selected using phage ~YeO3-12 as above and tested for phage ~R1-37 sensitivity (Table 2). Plasmids pAM200 and pRV12 did not trans-complement the mutation. The latter contains 1.4 kb upstream and 5.2 ...
... mutants. To test this, rough derivatives of previously constructed trans-complementation strains were selected using phage ~YeO3-12 as above and tested for phage ~R1-37 sensitivity (Table 2). Plasmids pAM200 and pRV12 did not trans-complement the mutation. The latter contains 1.4 kb upstream and 5.2 ...
Evolution at the Subgene Level: Domain Rearrangements in
... We present a novel phylogenomic pipeline for the architecture-aware reconstruction of gene evolution (fig. 3). The pipeline has three main stages: 1) identifying modules and module families from the genomic sequences, 2) clustering architectures into architecture families, and 3) reconstructing arch ...
... We present a novel phylogenomic pipeline for the architecture-aware reconstruction of gene evolution (fig. 3). The pipeline has three main stages: 1) identifying modules and module families from the genomic sequences, 2) clustering architectures into architecture families, and 3) reconstructing arch ...
Journal of Advanced Scientific Research MICROBIAL SPOILAGE OF
... presence in canned food portend possible spoilage if storage conditions become favourable due to abuse. Staphylococcus aureus and Pseudomonas are of human flora, they are known opportunistic pathogens. Contamination of canned foods could be via food producers/handlers and equipments. They are facult ...
... presence in canned food portend possible spoilage if storage conditions become favourable due to abuse. Staphylococcus aureus and Pseudomonas are of human flora, they are known opportunistic pathogens. Contamination of canned foods could be via food producers/handlers and equipments. They are facult ...
PROKARYOTES AND THE ORIGINS OF METABOLIC DIVERSITY
... ⇒ The domain Archaea includes the archaebacteria, the Bacteria includes the eubacteria, and the Eukarya includes all eukaryotic organisms. ⇒ The Eukarya and Archaea shared a common ancestor which lived more recently than the ancestor shared between the Archaea and Bacteria. • Although systematic deb ...
... ⇒ The domain Archaea includes the archaebacteria, the Bacteria includes the eubacteria, and the Eukarya includes all eukaryotic organisms. ⇒ The Eukarya and Archaea shared a common ancestor which lived more recently than the ancestor shared between the Archaea and Bacteria. • Although systematic deb ...
Cleavage of a model DNA replication fork by a Type I restriction
... Tris–HCl (pH 7.5), 2 mM ATP, 10 mM MgCl2, at 378C, for the indicated time, with or without 120 nM EcoR124I. The total reaction volume was 15 ml. This condition is identical to that used previously except for the MgCl2 concentration (33). Under the same condition, 5.3 nM of a circular DNA with a sing ...
... Tris–HCl (pH 7.5), 2 mM ATP, 10 mM MgCl2, at 378C, for the indicated time, with or without 120 nM EcoR124I. The total reaction volume was 15 ml. This condition is identical to that used previously except for the MgCl2 concentration (33). Under the same condition, 5.3 nM of a circular DNA with a sing ...
A Single Eubacterial Origin of Eukaryotic
... original PCR product to amplify almost the entire 59 region and to within 200 bp of the expected 39 end of the gene. Examination of preliminary sequence data from the G. lamblia Genome Project at The Josephine Bay Paul Center Web site at the Marine Biological Laboratory (www.bpc.mbl.edu) suggested t ...
... original PCR product to amplify almost the entire 59 region and to within 200 bp of the expected 39 end of the gene. Examination of preliminary sequence data from the G. lamblia Genome Project at The Josephine Bay Paul Center Web site at the Marine Biological Laboratory (www.bpc.mbl.edu) suggested t ...
The Effects of Variables on Microbial Growth in Wastewater
... 2. Organize students into laboratory groups of four. Students are to complete the following procedures for sampling and viewing the representative organisms. a) Half fill each of six culture dishes with boiled pond or spring water. Label the dishes A through F. With a dropper, add ten drops of each ...
... 2. Organize students into laboratory groups of four. Students are to complete the following procedures for sampling and viewing the representative organisms. a) Half fill each of six culture dishes with boiled pond or spring water. Label the dishes A through F. With a dropper, add ten drops of each ...
A mixed group ll/group III twintron in the Euglena
... The splicing of a 409 nucleotide intron from the Euglena gracilis chloroplast ribosomal protein S3 gene (rps3) was examined by cDNA cloning and sequencing, and northern hybridization. Based on the characterization of a partially spliced pre-mRNA, the intron was characterized as a 'mixed' twintron, c ...
... The splicing of a 409 nucleotide intron from the Euglena gracilis chloroplast ribosomal protein S3 gene (rps3) was examined by cDNA cloning and sequencing, and northern hybridization. Based on the characterization of a partially spliced pre-mRNA, the intron was characterized as a 'mixed' twintron, c ...
Intervention, integration and translation in obesity research: Genetic
... Arguably an even more important finding was that of the Lep gene and its product leptin, from the Greek for ‘thin’, The recessive gene mutation (Lepob – ob for obesity) had been linked to obesity and type 2 diabetes since the early 1950s, as was the mutant db of the leptin receptor gene (Lepr db ) [ ...
... Arguably an even more important finding was that of the Lep gene and its product leptin, from the Greek for ‘thin’, The recessive gene mutation (Lepob – ob for obesity) had been linked to obesity and type 2 diabetes since the early 1950s, as was the mutant db of the leptin receptor gene (Lepr db ) [ ...
The Effects of Variables on Microbial Growth in Wastewater
... 2. Organize students into laboratory groups of four. Students are to complete the following procedures for sampling and viewing the representative organisms. a) Half fill each of six culture dishes with boiled pond or spring water. Label the dishes A through F. With a dropper, add ten drops of each ...
... 2. Organize students into laboratory groups of four. Students are to complete the following procedures for sampling and viewing the representative organisms. a) Half fill each of six culture dishes with boiled pond or spring water. Label the dishes A through F. With a dropper, add ten drops of each ...
Significance of biocide usage and antimicrobial resistance in
... resistance to the QAC, benzalkonium chloride and antibiotic resistance genes. Six qacA/Bharbouring strains were also found to harbour the B-lactamase gene blaZ. In three of these strains the gene were located on the same plasmid. Studies involving biocides with specific target sites As stated previo ...
... resistance to the QAC, benzalkonium chloride and antibiotic resistance genes. Six qacA/Bharbouring strains were also found to harbour the B-lactamase gene blaZ. In three of these strains the gene were located on the same plasmid. Studies involving biocides with specific target sites As stated previo ...
Codon bias domains over bacterial chromosomes
... – Frequency of usage of the corresponding codon in the phage genome (+) – Frequency of usage of the corresponding codon in the host genome (-) – Difference of codon usage frequencies between phage and host genome (+) ...
... – Frequency of usage of the corresponding codon in the phage genome (+) – Frequency of usage of the corresponding codon in the host genome (-) – Difference of codon usage frequencies between phage and host genome (+) ...
What is PCR? - Cobb Learning
... intron based, or non-coding. • We pass introns and exons on from one generation to the next. ...
... intron based, or non-coding. • We pass introns and exons on from one generation to the next. ...
Quantifying the DNA Binding Properties of the Binuclear Ruthenium
... diameter of 2 nanometers and a base pair separation of 0.34 nanometers. The angle of its rotation results in the structure repeating every 10 base pairs.9 ...
... diameter of 2 nanometers and a base pair separation of 0.34 nanometers. The angle of its rotation results in the structure repeating every 10 base pairs.9 ...
Classifying Gene Expression Data using an Evolutionary Algorithm
... in labelling or detecting fluorescent dyes, and systematic biases in the expression level measurements (Quckenbush, 2002). Many of these factors make distinctions between differentially and constantly expressed genes difficult. The normalization process must be done on with the log-ratio values befo ...
... in labelling or detecting fluorescent dyes, and systematic biases in the expression level measurements (Quckenbush, 2002). Many of these factors make distinctions between differentially and constantly expressed genes difficult. The normalization process must be done on with the log-ratio values befo ...
Gene Selection For A Discriminant Microarray Data Analysis
... Breast Cancer Data Microarray experiments were carried out before and after chemotherapy on the same patient. Since these two samples are not independent, usual logistic regression can not be applied. We use paired case-control logistic regression. Two features: (1) each pair is essentially ...
... Breast Cancer Data Microarray experiments were carried out before and after chemotherapy on the same patient. Since these two samples are not independent, usual logistic regression can not be applied. We use paired case-control logistic regression. Two features: (1) each pair is essentially ...
Pseudomonas fluorescens can be used for bioremediation of textile
... cotton, etc. Dyes are recalcitrant molecules difficult to be degraded biologically. Heavy metals as cadmium, chromium, copper, lead, zinc are present in the dyes. Black et al. (1980) have demonstrated that aniline dyes could be mutagenic and carcinogenic to biota. These chemicals have been suggested ...
... cotton, etc. Dyes are recalcitrant molecules difficult to be degraded biologically. Heavy metals as cadmium, chromium, copper, lead, zinc are present in the dyes. Black et al. (1980) have demonstrated that aniline dyes could be mutagenic and carcinogenic to biota. These chemicals have been suggested ...
Linkage Disequilibrium and Inference of Ancestral
... integrates the effects of mutation, drift, and recombination in giving rise to a particular statistical association across sites. For example, in a population that is in steady state with respect to accumulation of neutral mutations, random drift, and recombination, the expected LD is E(r 2 ) p 1/(1 ...
... integrates the effects of mutation, drift, and recombination in giving rise to a particular statistical association across sites. For example, in a population that is in steady state with respect to accumulation of neutral mutations, random drift, and recombination, the expected LD is E(r 2 ) p 1/(1 ...
Parasitism and Mutualism in Wolbachia: What the
... the arthropods, antibiotic curing experiments suggest that in nematodes, Wolbachia infections are primarily beneficial to nematode fertility and larval development (Taylor et al. 2005). Further, the Wolbachia genome sequence from the filariid Brugia malayi suggests that these mutualists contribute e ...
... the arthropods, antibiotic curing experiments suggest that in nematodes, Wolbachia infections are primarily beneficial to nematode fertility and larval development (Taylor et al. 2005). Further, the Wolbachia genome sequence from the filariid Brugia malayi suggests that these mutualists contribute e ...
IEEE International Conference on Bioinformatics and Biomedicine
... November 9-12, 2015, Washington DC, USA ...
... November 9-12, 2015, Washington DC, USA ...
Phytopathology
... polymerase chain reaction (PCR) now also can be used as a confirmatory assay. Although the sensitivity of PCR may make its use advantageous, particularly if conducted with one of the realtime technologies (30) which alleviates the need for postamplification identification of PCR product, its widespr ...
... polymerase chain reaction (PCR) now also can be used as a confirmatory assay. Although the sensitivity of PCR may make its use advantageous, particularly if conducted with one of the realtime technologies (30) which alleviates the need for postamplification identification of PCR product, its widespr ...
unit-2 genetics of prokaryotes and eukaryotic
... chromosome or for finding out distances of these genes from centromere, this technique is called chromosome mapping. If linkage groups are already established in an organism, trisomics can be effectively used for assigning these linkage groups to specific chromosomes. Since the segregation ratio for ...
... chromosome or for finding out distances of these genes from centromere, this technique is called chromosome mapping. If linkage groups are already established in an organism, trisomics can be effectively used for assigning these linkage groups to specific chromosomes. Since the segregation ratio for ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.