Functional Genomics
... demand for algorithms (for example, see ref. 10) and relational databases that can integrate the information that will be obtained using very different tools. Figure 2 Understanding gene function. Full legend High resolution image and legend (61k) ...
... demand for algorithms (for example, see ref. 10) and relational databases that can integrate the information that will be obtained using very different tools. Figure 2 Understanding gene function. Full legend High resolution image and legend (61k) ...
A bacterial genome in flux: the twelve linear and nine circular
... plasmids, cp32-2 and cp32-5, in other cultures of isolate B31 that are not present in B31 culture MI. These 10 new plasmid DNA sequences, along with those previously published in Fraser et al. (1997), account for all of the random sequence generated by this genome sequencing project. Because of the ...
... plasmids, cp32-2 and cp32-5, in other cultures of isolate B31 that are not present in B31 culture MI. These 10 new plasmid DNA sequences, along with those previously published in Fraser et al. (1997), account for all of the random sequence generated by this genome sequencing project. Because of the ...
1 Sequential elimination of major-effect contributors
... confounding effects of major loci and epistasis. Therefore, to avoid confounding effects of major loci, epistasis and sample size, we used a targeted backcross mapping strategy that genetically eliminated the effect of a previously identified major QTL underlying high-temperature growth (Htg) in yea ...
... confounding effects of major loci and epistasis. Therefore, to avoid confounding effects of major loci, epistasis and sample size, we used a targeted backcross mapping strategy that genetically eliminated the effect of a previously identified major QTL underlying high-temperature growth (Htg) in yea ...
Bacteriophage A cloning system for the construction of
... phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containing single recognition sequences for the restriction enzymes B ...
... phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containing single recognition sequences for the restriction enzymes B ...
University of Groningen Methionine
... and utilization. The putative regulatory site of CmhR (5¢ TATAGTTTSAAACTATA-3¢ , where S denotes G/C/A) in the promoter regions of its regulon genes is predicted and confirmed by promoter mutational experiments. This site is also found to be highly conserved in other pneumococcal strains and strepto ...
... and utilization. The putative regulatory site of CmhR (5¢ TATAGTTTSAAACTATA-3¢ , where S denotes G/C/A) in the promoter regions of its regulon genes is predicted and confirmed by promoter mutational experiments. This site is also found to be highly conserved in other pneumococcal strains and strepto ...
Package `fcros`
... function fcros2() performs a pairwise comparison of samples from each table to obtain fold changes. The fold changes are sorted, their rank values are combined and then used to associate statistic with genes/probes. The function to use with a dataset from two biological condition samples. The datase ...
... function fcros2() performs a pairwise comparison of samples from each table to obtain fold changes. The fold changes are sorted, their rank values are combined and then used to associate statistic with genes/probes. The function to use with a dataset from two biological condition samples. The datase ...
An Introduction to Streptomyces
... due to their diversity and complex life cycles. Organisms are assigned to this class on the basis of their chemotaxonomy, their high G+C context and the similarities in the sequences of their 16S ribosomal ribonucleic acid (Hain, 1997). In the early steps of microbiology, many organisms now belongin ...
... due to their diversity and complex life cycles. Organisms are assigned to this class on the basis of their chemotaxonomy, their high G+C context and the similarities in the sequences of their 16S ribosomal ribonucleic acid (Hain, 1997). In the early steps of microbiology, many organisms now belongin ...
fulltext
... Preparation and amplification of DNA (II, III) ........................................ 26 Determination of the viability of bacterial cells (I, III, IV) ...................... 27 Antibiotic susceptibility testing (II, III) ................................................... 27 Determination of s ...
... Preparation and amplification of DNA (II, III) ........................................ 26 Determination of the viability of bacterial cells (I, III, IV) ...................... 27 Antibiotic susceptibility testing (II, III) ................................................... 27 Determination of s ...
Structural changes following the reversal of a Y chromosome to an
... translocations can also reverse sex chromosomes back into autosomes, which is especially intriguing given that autosomes and sex chromosomes differ in gene regulation and chromatin environment. While researchers are beginning to understand X chromosomes reversals to autosomes at a genomic level, it ...
... translocations can also reverse sex chromosomes back into autosomes, which is especially intriguing given that autosomes and sex chromosomes differ in gene regulation and chromatin environment. While researchers are beginning to understand X chromosomes reversals to autosomes at a genomic level, it ...
Antibiotic resistance in microbes
... cellent example of this relationship between producer and clinical isolate (or an extraordinary coincidence!). Glycopeptide resistance in the enterococci is due to a cluster of genes that encode the synthesis of a novel cell envelope component, D-ala-D-lac that replaces the Dala-D-ala moiety normall ...
... cellent example of this relationship between producer and clinical isolate (or an extraordinary coincidence!). Glycopeptide resistance in the enterococci is due to a cluster of genes that encode the synthesis of a novel cell envelope component, D-ala-D-lac that replaces the Dala-D-ala moiety normall ...
Complete comparative genomic analysis of two field isolates of
... Analysis of all variations showed that 398 point mutations, six insertions totalling 30 bp and 20 deletions totalling 1326 bp occur in predicted ORFs; 65 point mutations, three insertions (13 bp) and five deletions (102 bp) occur in intergenic regions; and 58 point mutations, two insertions (79 bp) ...
... Analysis of all variations showed that 398 point mutations, six insertions totalling 30 bp and 20 deletions totalling 1326 bp occur in predicted ORFs; 65 point mutations, three insertions (13 bp) and five deletions (102 bp) occur in intergenic regions; and 58 point mutations, two insertions (79 bp) ...
African green monkeys - Journal of General Virology
... mangabeys, and the fact that the geographical spread of HIV-2 epidemics corresponds to the natural habitat of sooty mangabeys in West Africa, is an additional argument in favour of cross-species transmission (Marx et al., 1991 ; Gao et at., 1992). Based on genomic sequences, five genetically distinc ...
... mangabeys, and the fact that the geographical spread of HIV-2 epidemics corresponds to the natural habitat of sooty mangabeys in West Africa, is an additional argument in favour of cross-species transmission (Marx et al., 1991 ; Gao et at., 1992). Based on genomic sequences, five genetically distinc ...
African green monkeys (Cercopithecus aethiops sabaeus) in the wild
... mangabeys, and the fact that the geographical spread of HIV-2 epidemics corresponds to the natural habitat of sooty mangabeys in West Africa, is an additional argument in favour of cross-species transmission (Marx et al., 1991 ; Gao et at., 1992). Based on genomic sequences, five genetically distinc ...
... mangabeys, and the fact that the geographical spread of HIV-2 epidemics corresponds to the natural habitat of sooty mangabeys in West Africa, is an additional argument in favour of cross-species transmission (Marx et al., 1991 ; Gao et at., 1992). Based on genomic sequences, five genetically distinc ...
Multiple Domains Exist within the Upstream Activator
... pSaHi69 all contain half of the palindrome and are inactive in tobacco calli. The palindrome must therefore be complete in order for the UAS to function. Oligonucleotides corresponding to either half of the palindrome, pHPPD and ...
... pSaHi69 all contain half of the palindrome and are inactive in tobacco calli. The palindrome must therefore be complete in order for the UAS to function. Oligonucleotides corresponding to either half of the palindrome, pHPPD and ...
ADVANCING JUSTICE THROUGH DNA TECHNOLOGY
... Statistics (BJS) found that between 1997 and 2000, DNA laboratories experienced a 73% increase in casework and a 135% increase in their casework backlogs. Many casework samples go unanalyzed for lack of a suspect to which to compare the biological evidence from the crime scene. These are often refer ...
... Statistics (BJS) found that between 1997 and 2000, DNA laboratories experienced a 73% increase in casework and a 135% increase in their casework backlogs. Many casework samples go unanalyzed for lack of a suspect to which to compare the biological evidence from the crime scene. These are often refer ...
Chromosomes Carrying Meiotic Avoidance Loci
... contain a small deletion or translocation as a result of g-irradiation (Supplemental Fig. S1; Koltunow et al., 2011b). These four SCAR markers were used to screen an H. praealtum (R35) bacterial artificial chromosome (BAC) library in order to isolate genomic sequences associated with the LOA locus. ...
... contain a small deletion or translocation as a result of g-irradiation (Supplemental Fig. S1; Koltunow et al., 2011b). These four SCAR markers were used to screen an H. praealtum (R35) bacterial artificial chromosome (BAC) library in order to isolate genomic sequences associated with the LOA locus. ...
TR-00-04 - Svensk Kärnbränslehantering AB
... sampled Palmottu groundwater is coupled with the reduction activities of the found microorganisms. In borehole R387, the distribution of SRB and IRB showed an inverse correlation with dissolved uranium. An attempt to mimic the groundwater situation in culture tubes inoculated with enrichment culture ...
... sampled Palmottu groundwater is coupled with the reduction activities of the found microorganisms. In borehole R387, the distribution of SRB and IRB showed an inverse correlation with dissolved uranium. An attempt to mimic the groundwater situation in culture tubes inoculated with enrichment culture ...
Genome duplications and accelerated evolution of
... entire genome and the subsequent loss of individual genes. The presumed conserved and important roles of these genes in tetrapods during development led to the general assumption that Hox cluster architecture had remained unchanged since the last common ancestor of all jawed vertebrates. But recent ...
... entire genome and the subsequent loss of individual genes. The presumed conserved and important roles of these genes in tetrapods during development led to the general assumption that Hox cluster architecture had remained unchanged since the last common ancestor of all jawed vertebrates. But recent ...
Castric et al. (MBE 2010) - GEPV
... these two effects remains to be determined, both theoretically and empirically. A second distinctive feature of the molecular evolution of SI genes is the role played by recombination and/or gene conversion. Indeed, whether recombination occurs at all in the genomic region of the S-locus is still a ...
... these two effects remains to be determined, both theoretically and empirically. A second distinctive feature of the molecular evolution of SI genes is the role played by recombination and/or gene conversion. Indeed, whether recombination occurs at all in the genomic region of the S-locus is still a ...
Alarmingly High Segregation Frequencies of Quinolone Resistance
... Antibiotic resistance poses a major threat to human health. It is therefore important to characterize the frequency of resistance within natural bacterial environments. Many studies have focused on characterizing the frequencies with which horizontally acquired resistance genes segregate within natu ...
... Antibiotic resistance poses a major threat to human health. It is therefore important to characterize the frequency of resistance within natural bacterial environments. Many studies have focused on characterizing the frequencies with which horizontally acquired resistance genes segregate within natu ...
Transmission-ratio distortion in the Framingham Heart Study | BMC
... family-based association) is that Mendel's law of segregation is obeyed. Transmission-ratio distortion (TRD) refers to the deviation from the expected Mendelian inheritance of alleles. Violation of this assumption could result in false-positive linkage, particularly within ‘affected-only’ or ‘non-pa ...
... family-based association) is that Mendel's law of segregation is obeyed. Transmission-ratio distortion (TRD) refers to the deviation from the expected Mendelian inheritance of alleles. Violation of this assumption could result in false-positive linkage, particularly within ‘affected-only’ or ‘non-pa ...
Introduction to Molecular Diagnostics
... sequences have a known association with a specific health condition or disease, they are often referred to as “biomarkers”” because they are markers of that condition or disease. Molecular diagnostics are the tools that are driving the continuing discovery of biomarkers at the research level, which ...
... sequences have a known association with a specific health condition or disease, they are often referred to as “biomarkers”” because they are markers of that condition or disease. Molecular diagnostics are the tools that are driving the continuing discovery of biomarkers at the research level, which ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
... our homes, hotels, restaurant kitchens, shops, supermarkets and bars, spreading diseases which can prove fatal to humans. Many cases of food poisoning are known to be as a result of cockroach contamination. Cockroaches have survived on the earth for more than 300 million years virtually without chan ...
... our homes, hotels, restaurant kitchens, shops, supermarkets and bars, spreading diseases which can prove fatal to humans. Many cases of food poisoning are known to be as a result of cockroach contamination. Cockroaches have survived on the earth for more than 300 million years virtually without chan ...
Open Questions on the Origin of Eukaryotes
... was likely present, opening the possibility for some form of sexual reproduction. The genome contained introns, making necessary a splicing system, likely integrated in a sophisticated gene regulation machinery that also included the activity of small non-coding RNAs and RNA interference. This list ...
... was likely present, opening the possibility for some form of sexual reproduction. The genome contained introns, making necessary a splicing system, likely integrated in a sophisticated gene regulation machinery that also included the activity of small non-coding RNAs and RNA interference. This list ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.