Comparison of the Detection Limits of the Culture and PCR Methods
... Arch Pediatr Infect Dis. inpress(inpress):e38888. ...
... Arch Pediatr Infect Dis. inpress(inpress):e38888. ...
Investigating the environmental transport of human pharmaceuticals
... EA study analytical methods were developed for three substances, tamoxifen, dextropropoxyphene and lofepramine (Hilton and Thomas, 2003). Once a drug is ingested and metabolised in the body a variable proportion is excreted as a conjugate, very little is excreted as the free compound. Studies on the ...
... EA study analytical methods were developed for three substances, tamoxifen, dextropropoxyphene and lofepramine (Hilton and Thomas, 2003). Once a drug is ingested and metabolised in the body a variable proportion is excreted as a conjugate, very little is excreted as the free compound. Studies on the ...
Rapid Diversification of RNase A Superfamily Ribonucleases from
... these sequences were also identified by PCR amplification but not reported in the studies performed by Liao et al. (2000). The amino terminal “Q” of these novel sequences has been inferred based on similarity to the amino acid sequence of rc-RNase (rc-RN), whose terminus was determined directly via ...
... these sequences were also identified by PCR amplification but not reported in the studies performed by Liao et al. (2000). The amino terminal “Q” of these novel sequences has been inferred based on similarity to the amino acid sequence of rc-RNase (rc-RN), whose terminus was determined directly via ...
SNP
... Polymorphisms (SNPs) the variant sequence type has a frequency of at least 1% in the population. high frequency of SNPs in human genome: estimated ~1 SNP/Kb. ...
... Polymorphisms (SNPs) the variant sequence type has a frequency of at least 1% in the population. high frequency of SNPs in human genome: estimated ~1 SNP/Kb. ...
Comparison of the Detection Limits of the Culture and PCR Methods
... stool samples of patients with gastroenteritis in developing countries (28-30). These rates are lower than those reported for developed countries (4, 31). Despite differences in geographic area and culture in these countries, inaccurate results could be obtained due to the need for individual equipm ...
... stool samples of patients with gastroenteritis in developing countries (28-30). These rates are lower than those reported for developed countries (4, 31). Despite differences in geographic area and culture in these countries, inaccurate results could be obtained due to the need for individual equipm ...
Water SA
... are time consuming and special reagents, culture media and a high degree of technical skill are required in their application (Bartie et al., 2001) because it is difficult to isolate Legionella in waters with high levels of other heterotrophic bacteria. However, until now, standard culture methods h ...
... are time consuming and special reagents, culture media and a high degree of technical skill are required in their application (Bartie et al., 2001) because it is difficult to isolate Legionella in waters with high levels of other heterotrophic bacteria. However, until now, standard culture methods h ...
MOLECULAR CYTOGENETIC ANALYSES IN WHEAT AND
... genome of DNA fragments measuring only a few base pairs in length. Molecular cytogenetic techniques make it possible to study the desired DNA segments within the chromosome itself. Continual improvements in the methods and the combination of various molecular cytogenetic techniques allow the physica ...
... genome of DNA fragments measuring only a few base pairs in length. Molecular cytogenetic techniques make it possible to study the desired DNA segments within the chromosome itself. Continual improvements in the methods and the combination of various molecular cytogenetic techniques allow the physica ...
Potential of promotion of alleles by genome editing for improving
... Overall hypothesis of GS2.0 • Sequence data has huge potential in livestock and plant breeding • Huge volumes of sequence needed to realize potential • Individual breeding programs with 1 million animals with sequence information: – Will be normal “by the end of the decade” – We have project in pla ...
... Overall hypothesis of GS2.0 • Sequence data has huge potential in livestock and plant breeding • Huge volumes of sequence needed to realize potential • Individual breeding programs with 1 million animals with sequence information: – Will be normal “by the end of the decade” – We have project in pla ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... of a soluble form of CR1 in plasma (5). This regulatory capacity also suggested that CR1 was related to factor H and C4-binding protein (C4BP),' and these three proteins were subsequently shown to share a repeating 60-amino-acid motif in their primary sequence and to be genetically linked (6-9). An ...
... of a soluble form of CR1 in plasma (5). This regulatory capacity also suggested that CR1 was related to factor H and C4-binding protein (C4BP),' and these three proteins were subsequently shown to share a repeating 60-amino-acid motif in their primary sequence and to be genetically linked (6-9). An ...
The msh2 Gene of Schizosaccharomyces pombe Is
... homology to the Msh2 subgroup. msh2 disruption gives rise to increased mitotic mutation rates and increased levels of postmeiotic segregation of genetic markers. In bandshift assays performed with msh2D cell extracts, a general mismatch-binding activity is absent. By complementation assays, we showe ...
... homology to the Msh2 subgroup. msh2 disruption gives rise to increased mitotic mutation rates and increased levels of postmeiotic segregation of genetic markers. In bandshift assays performed with msh2D cell extracts, a general mismatch-binding activity is absent. By complementation assays, we showe ...
Correction to “Frequency of Undetected CYP2D6
... Frequency of hybrid genes per sample type The frequency of hybrids genes in CYP2D6*4 and CYP2D6*10 samples is reported for both heterozygous or homozygous and heterozygous alone. We did not determine whether one or both chromosomes contained a hybrid tandem in homozygous samples. For this reason, to ...
... Frequency of hybrid genes per sample type The frequency of hybrids genes in CYP2D6*4 and CYP2D6*10 samples is reported for both heterozygous or homozygous and heterozygous alone. We did not determine whether one or both chromosomes contained a hybrid tandem in homozygous samples. For this reason, to ...
Complete genome sequence of the thermophilic Thermus sp
... scotoductus SA-01) were selected for the comparison. The genome of strain HB27 consists of a chromosome (1.89 Mb) and a megaplasmid (0.23 Mb). On the other hand, strain HB8 has a chromosome of 1.85 Mb, a megaplasmid (0.26 Mb) and a plasmid (9.3 kb) [5]. The genome of T. scotoductus includes a 2.3 Mb ...
... scotoductus SA-01) were selected for the comparison. The genome of strain HB27 consists of a chromosome (1.89 Mb) and a megaplasmid (0.23 Mb). On the other hand, strain HB8 has a chromosome of 1.85 Mb, a megaplasmid (0.26 Mb) and a plasmid (9.3 kb) [5]. The genome of T. scotoductus includes a 2.3 Mb ...
what is alignment? - UWI St. Augustine
... Basis for Sequence comparison • Theory of evolution: – gene sequences have evolved/derived from a common ancestor – trace history of mutations/evolutionary changes ...
... Basis for Sequence comparison • Theory of evolution: – gene sequences have evolved/derived from a common ancestor – trace history of mutations/evolutionary changes ...
5 Genetic Analysis of Kidney Disease in Mice
... genetic and environmental factors. Because of structural homology between the mouse, rat, and human genomes, quantitative trait loci (QTL) detected in rodent models of disease often predict the location of human QTL. Several CKD QTL identified in rats are homologous to human CKD QTL (Korstanje and D ...
... genetic and environmental factors. Because of structural homology between the mouse, rat, and human genomes, quantitative trait loci (QTL) detected in rodent models of disease often predict the location of human QTL. Several CKD QTL identified in rats are homologous to human CKD QTL (Korstanje and D ...
BROWSING GENES AND GENOMES WITH ENSEMBL
... It doesn’t look like there is a clone that contains the complete BRCA2 gene. For example clone RP11-37E23 contains most of the gene, but not its very 3’ end. (d) 8 Click [Configure this page] in the side menu. 8 Type ‘refseq’ in the ‘Find a track’ text box. 8 Select ‘RefSeq import – Expanded wit ...
... It doesn’t look like there is a clone that contains the complete BRCA2 gene. For example clone RP11-37E23 contains most of the gene, but not its very 3’ end. (d) 8 Click [Configure this page] in the side menu. 8 Type ‘refseq’ in the ‘Find a track’ text box. 8 Select ‘RefSeq import – Expanded wit ...
Assaying … promoter activity
... …the plasmid integrated into the genome by homologous recombination with the nimB and ORF5468 gene. We tested the stability of the integrated plasmid via a true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic select ...
... …the plasmid integrated into the genome by homologous recombination with the nimB and ORF5468 gene. We tested the stability of the integrated plasmid via a true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic select ...
Triplet repeat primed PCR assay for the screening and diagnosis of
... neurodegenerative diseases caused by dynamic expansions of trinucleotide repeats in different genes (Table 1). These diseases are characterized by progressive incoordination of gait, poor coordination of hands, speech, and eye movements. They are either inherited recessively (FA) or dominantly (SCA) ...
... neurodegenerative diseases caused by dynamic expansions of trinucleotide repeats in different genes (Table 1). These diseases are characterized by progressive incoordination of gait, poor coordination of hands, speech, and eye movements. They are either inherited recessively (FA) or dominantly (SCA) ...
id telt
... • xTAG® GPP is the first diagnostic to offer detection of 15 major gastrointestinal pathogens in a single test • Results within 5 hours for timely, well-informed treatment decisions and better patient care • Fast turn-around time and multiplex testing mean better use of time and human resources ...
... • xTAG® GPP is the first diagnostic to offer detection of 15 major gastrointestinal pathogens in a single test • Results within 5 hours for timely, well-informed treatment decisions and better patient care • Fast turn-around time and multiplex testing mean better use of time and human resources ...
Proof-of-principle rapid noninvasive prenatal diagnosis
... size (Supplemental Table 4). Therefore, we sought a larger haplotype sequence to aid fetal diagnosis by mapping a consensus N370S founder region surrounding the GBA gene. Fine mapping of the consensus AJ N370S founder haplotype region. To fine map the N370S founder region, we sequenced 7 unrelated h ...
... size (Supplemental Table 4). Therefore, we sought a larger haplotype sequence to aid fetal diagnosis by mapping a consensus N370S founder region surrounding the GBA gene. Fine mapping of the consensus AJ N370S founder haplotype region. To fine map the N370S founder region, we sequenced 7 unrelated h ...
Frequent, independent transfers of a catabolic gene from bacteria to
... of a particular species can differ by large fractions of their genome [2–6]. Long thought to be a prokaryote specialty, HGT is now recognized as a mechanism of genetic innovation in eukaryotes as well [7–10]. Genome analysis of eukaryotes revealed that several genes had been horizontally transferred ...
... of a particular species can differ by large fractions of their genome [2–6]. Long thought to be a prokaryote specialty, HGT is now recognized as a mechanism of genetic innovation in eukaryotes as well [7–10]. Genome analysis of eukaryotes revealed that several genes had been horizontally transferred ...
Systematic and Applied Microbiology - digital
... codes for a putative tyrosine decarboxilase in E. faecium. In order to check the presence of the gene coding for this protein in the E. faecium strains under study, two oligonucleotides were designed. We used primer 57 (5´-ATGAGTGAATCATTGTCG3´) and 58 (5´-TTATTTTGCTTCGCTTGCC-3´) to amplify a 1.9 kb ...
... codes for a putative tyrosine decarboxilase in E. faecium. In order to check the presence of the gene coding for this protein in the E. faecium strains under study, two oligonucleotides were designed. We used primer 57 (5´-ATGAGTGAATCATTGTCG3´) and 58 (5´-TTATTTTGCTTCGCTTGCC-3´) to amplify a 1.9 kb ...
Set - people.vcu.edu
... • The interface facilitates operations on sets of genes and sequences • The interface puts at your disposal powerful tools (that already exist), without the need to figure out a different computer environment • Taken together, these capabilities make possible a focus by those not particularly adept ...
... • The interface facilitates operations on sets of genes and sequences • The interface puts at your disposal powerful tools (that already exist), without the need to figure out a different computer environment • Taken together, these capabilities make possible a focus by those not particularly adept ...
Phytoplankton community structure defined by key
... productive period (May / June / July) at ebb tide. The main objective of this study was ...
... productive period (May / June / July) at ebb tide. The main objective of this study was ...
Drosophila Muller F Elements Maintain a Distinct Set of Genomic
... using a sliding window of 1 kb with a step size of 500 bp. Gene annotations This comparative analysis used the high-quality D. melanogaster gene annotations (release 5.50) produced by FlyBase as reference (Marygold et al. 2013). The annotation protocol has been described previously (Shaffer et al. 2 ...
... using a sliding window of 1 kb with a step size of 500 bp. Gene annotations This comparative analysis used the high-quality D. melanogaster gene annotations (release 5.50) produced by FlyBase as reference (Marygold et al. 2013). The annotation protocol has been described previously (Shaffer et al. 2 ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.