Polycomb Group silencers collaborate with Notch pathway to cause
... Mutant discs are oddly shaped when compared with WT discs, but the resulting adults have largely normal eyes. RT-PCR experiments were performed using RNA isolated from third instar larvae collected from GS88A8/hsp70-Gal4 heat-shocked (+) or without heat-shock (—). We noted that RT-PCR experiments sh ...
... Mutant discs are oddly shaped when compared with WT discs, but the resulting adults have largely normal eyes. RT-PCR experiments were performed using RNA isolated from third instar larvae collected from GS88A8/hsp70-Gal4 heat-shocked (+) or without heat-shock (—). We noted that RT-PCR experiments sh ...
Alternative Splicing in Higher Plants
... with the annotation of the genome and the relatively low gene number • The complexity of human development is difficult to explain using only 30,000 genes • Humans have had a recent expansion of the transposable element Alu in the genome - 4% of human protein coding genes contain at least one Alu - ...
... with the annotation of the genome and the relatively low gene number • The complexity of human development is difficult to explain using only 30,000 genes • Humans have had a recent expansion of the transposable element Alu in the genome - 4% of human protein coding genes contain at least one Alu - ...
Gene conversion and purifying selection shape nucleotide variation
... When a sample from a male showed two nucleotide peaks at one or more sites (“heterozygous” sites) in the L or M opsin gene, the individual should have two or more loci of the gene with different sequences. In this case, we conservatively inferred two loci for this gene. When a female showed heterozy ...
... When a sample from a male showed two nucleotide peaks at one or more sites (“heterozygous” sites) in the L or M opsin gene, the individual should have two or more loci of the gene with different sequences. In this case, we conservatively inferred two loci for this gene. When a female showed heterozy ...
Evaluation of Three Methods for DNA Fingerprinting
... Warsaw University of Life Sciences, Warsaw, Poland Received 24 October 2007, revised 14 January 2008, accepted 12 February 2008 Abstract Phenotypic approaches based on metabolic and biological characteristics of Corynebacterium pseudotuberculosis have been limited due to insufficient discrimination ...
... Warsaw University of Life Sciences, Warsaw, Poland Received 24 October 2007, revised 14 January 2008, accepted 12 February 2008 Abstract Phenotypic approaches based on metabolic and biological characteristics of Corynebacterium pseudotuberculosis have been limited due to insufficient discrimination ...
Genome-wide search for asthma susceptibility loci in a founder
... evidence for linkage between genome-wide markers and four diagnostic groups was assessed using the LR test (26). The four diagnostic groups were: (1) ‘strict’ asthma (group D); (2) BHR (groups C + D); (3) asthma symptoms (groups B + D); and (4) ‘loose’ asthma (groups B + C + D). All markers showing ...
... evidence for linkage between genome-wide markers and four diagnostic groups was assessed using the LR test (26). The four diagnostic groups were: (1) ‘strict’ asthma (group D); (2) BHR (groups C + D); (3) asthma symptoms (groups B + D); and (4) ‘loose’ asthma (groups B + C + D). All markers showing ...
Microbiological and Immunological Investigation of adult patients
... Sinusitis is one of the common diseases both in developed and undeveloped countries including Iraq and it is one of the major causes for longstanding morbidity and suffering of patient.(1)Paranasal sinuses are considered as part of the upper respiratory tract and they are composed of four pairs sinu ...
... Sinusitis is one of the common diseases both in developed and undeveloped countries including Iraq and it is one of the major causes for longstanding morbidity and suffering of patient.(1)Paranasal sinuses are considered as part of the upper respiratory tract and they are composed of four pairs sinu ...
Phylogenetic Network and Physicochemical Properties of
... the average hydropathy of each 19-amino acid segment to the mean of all segments for the respective gene. The average Kyte-Doolittle hydropathy index of 19 neighboring amino acids was calculated for each amino acid position according to the MITOMAP reference sequence and by reference to the pepinfo ...
... the average hydropathy of each 19-amino acid segment to the mean of all segments for the respective gene. The average Kyte-Doolittle hydropathy index of 19 neighboring amino acids was calculated for each amino acid position according to the MITOMAP reference sequence and by reference to the pepinfo ...
thesis - Tel Archives ouvertes
... background, methods and literature, while following three chapters present an analysis of datas and results. The first chapter gives an overview of male reproductive system with a detail explanation of testicular histology and organization, the second chapter provides definition of different steps o ...
... background, methods and literature, while following three chapters present an analysis of datas and results. The first chapter gives an overview of male reproductive system with a detail explanation of testicular histology and organization, the second chapter provides definition of different steps o ...
Gene transfer in bacteria - McGraw Hill Higher Education
... colonies from one plate to another (see Fig 7.5a) Mutagens – used to increase the frequency of mutations (see Fig 7.10) Enrichment – increases the proportion of mutant cells • e.g. Penicillin kills only cells that are dividing but not cells that are unable to divide (Fig. 14.4) ...
... colonies from one plate to another (see Fig 7.5a) Mutagens – used to increase the frequency of mutations (see Fig 7.10) Enrichment – increases the proportion of mutant cells • e.g. Penicillin kills only cells that are dividing but not cells that are unable to divide (Fig. 14.4) ...
Mapping of the Recessive White Locus and
... information (UCSC Genome Browser on Chicken; alignment of AB023291 and chromosome 1:179545508 to 179595094; Table 1). The PCR was performed in a total volume of 15 L containing 20 ng of genomic DNA, 6.25 pmol of each primer, 0.2 mM each deoxynucleoside triphosphate (dNTP), 10 mM TrisⴢHCl (pH 8.3), ...
... information (UCSC Genome Browser on Chicken; alignment of AB023291 and chromosome 1:179545508 to 179595094; Table 1). The PCR was performed in a total volume of 15 L containing 20 ng of genomic DNA, 6.25 pmol of each primer, 0.2 mM each deoxynucleoside triphosphate (dNTP), 10 mM TrisⴢHCl (pH 8.3), ...
WormBase Advisory Board Meeting RNAi
... ≈ Phenotype annotations are associated with molecular information for alleles, transgenes, and RNAi objects that permit mapping these objects to the genome. ≈ High-level phenotype annotations associated with RNAi objects are automatically converted to GO terms (RNAi2GO) and associated with gene obje ...
... ≈ Phenotype annotations are associated with molecular information for alleles, transgenes, and RNAi objects that permit mapping these objects to the genome. ≈ High-level phenotype annotations associated with RNAi objects are automatically converted to GO terms (RNAi2GO) and associated with gene obje ...
Genomic Organization of Evolutionarily Correlated Genes in
... widen the scope of co-function by analyzing a benchmark data set consisting of 2254 proteincoding genes contributing to 22,500 gene pairs. 7 These pairs had been identified by comparing 105 bacterial genomes on the basis of two types of evolutionary correlations: 7 a tendency to be located close toge ...
... widen the scope of co-function by analyzing a benchmark data set consisting of 2254 proteincoding genes contributing to 22,500 gene pairs. 7 These pairs had been identified by comparing 105 bacterial genomes on the basis of two types of evolutionary correlations: 7 a tendency to be located close toge ...
Periodontal microbial ecology
... Earth that potentially could have colonized the oral cavity. Clearly, potent forces control the establishment of the oral microbiota, govern its composition and influence its re-development once the ecosystem has been disturbed. It is the intent of this section to describe some of the factors that i ...
... Earth that potentially could have colonized the oral cavity. Clearly, potent forces control the establishment of the oral microbiota, govern its composition and influence its re-development once the ecosystem has been disturbed. It is the intent of this section to describe some of the factors that i ...
Module 5: Alternative Open Reading Frame
... over the first nucleotide of the highlighted in the start codon and a popup box will show up that has the nucleotide number indicated. Make a note of the number. Scroll down the page until you come to the highlighted stop codon in the same reading frame. Hover your cursor over the LAST nucleotide in ...
... over the first nucleotide of the highlighted in the start codon and a popup box will show up that has the nucleotide number indicated. Make a note of the number. Scroll down the page until you come to the highlighted stop codon in the same reading frame. Hover your cursor over the LAST nucleotide in ...
Package `acde` - USTC Open Source Software Mirror
... Maintainer Juan Pablo Acosta
Description This package provides a multivariate inferential analysis
method for detecting differentially expressed genes in gene
expression data. It uses artificial components, close to the
data's principal components but with an exact interpreta ...
... Maintainer Juan Pablo Acosta
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
... analyses revealed that the 16S rDNA sequences of strains of each of these pathogens were identical or nearly identical. Differences between the three phytoplasmas ranged from 1?0 to 1?5 % of nucleotide positions and were thus below the recommended threshold of 2?5 % for assigning species rank to phy ...
... analyses revealed that the 16S rDNA sequences of strains of each of these pathogens were identical or nearly identical. Differences between the three phytoplasmas ranged from 1?0 to 1?5 % of nucleotide positions and were thus below the recommended threshold of 2?5 % for assigning species rank to phy ...
Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus
... and endophytic, setting it apart from other acetic acid bacteria. It is often found in association with sugar cane where it stimulates plant growth through the secretion of auxin-like compounds, notably indole acetic acid (IAA) [29,30]. No indolepyruvate decarboxylases could be identified on the G. ...
... and endophytic, setting it apart from other acetic acid bacteria. It is often found in association with sugar cane where it stimulates plant growth through the secretion of auxin-like compounds, notably indole acetic acid (IAA) [29,30]. No indolepyruvate decarboxylases could be identified on the G. ...
Read the Nobel Lecture
... happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an enzyme that actively elongated telomeres, we might be able to detect it ...
... happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an enzyme that actively elongated telomeres, we might be able to detect it ...
Cot-1 banding of human chromosomes using fluorescence
... Human Cot-1 DNA prepared from placental DNA is known to be highly enriched in repetitive sequences such as the AluI and KpnI family members (Britten et at., 1974; Weiner et al., 1986; Nisson et al., 199I). It is conventionally used to suppress hybridization signals from repetitive D N A sequences pr ...
... Human Cot-1 DNA prepared from placental DNA is known to be highly enriched in repetitive sequences such as the AluI and KpnI family members (Britten et at., 1974; Weiner et al., 1986; Nisson et al., 199I). It is conventionally used to suppress hybridization signals from repetitive D N A sequences pr ...
Analyzing microRNA Data and Integrating microRNA with Gene
... Scenario: You have only gene expression results (or a list of genes of interest) and are interested in identifying which microRNAs might regulate the significant genes in that experiment. Using a database like TargetScan, you can create a list of microRNAs that are statistically predicted to regulat ...
... Scenario: You have only gene expression results (or a list of genes of interest) and are interested in identifying which microRNAs might regulate the significant genes in that experiment. Using a database like TargetScan, you can create a list of microRNAs that are statistically predicted to regulat ...
human genome research
... gene disorders such as cystic fibrosis, but also of how genes interact with environmental factors and contribute to a range of other diseases including cancers, heart disease, and diabetes. Once the role of gene sequences in these and other types of disease are known, the way will be opened for a ne ...
... gene disorders such as cystic fibrosis, but also of how genes interact with environmental factors and contribute to a range of other diseases including cancers, heart disease, and diabetes. Once the role of gene sequences in these and other types of disease are known, the way will be opened for a ne ...
The Comparison of Transcriptomes Undergoing Waterlogging at the
... Two DGE libraries were constructed using total RNA from the treatment and the control groups using an Illumina Digital Gene Expression Tag Profiling Kit according to the manufacturer's protocol (Version 2.1B). These two libraries underwent Illumina proprietary sequencing chip for ...
... Two DGE libraries were constructed using total RNA from the treatment and the control groups using an Illumina Digital Gene Expression Tag Profiling Kit according to the manufacturer's protocol (Version 2.1B). These two libraries underwent Illumina proprietary sequencing chip for ...
draft - IC
... the genome shu e around, has been studied since shortly after the very advent of genetics [1, 2, 3]. With the increased availability of whole genome sequences, gene order data have been used to estimate the evolutionary distance between present-day genomes and to reconstruct the gene order of ancest ...
... the genome shu e around, has been studied since shortly after the very advent of genetics [1, 2, 3]. With the increased availability of whole genome sequences, gene order data have been used to estimate the evolutionary distance between present-day genomes and to reconstruct the gene order of ancest ...
Genome Visualisation and Annotation Tools: Artemis and ACT
... Feature. A window will appear containing all the annotation that is associated with that CDS. The format for this information is constrained by that which can be submitted to the EMBL database where it is stored within ‘keys’ and ‘Qualifiers’ see Appendix II. Viewing amino acid or protein sequence C ...
... Feature. A window will appear containing all the annotation that is associated with that CDS. The format for this information is constrained by that which can be submitted to the EMBL database where it is stored within ‘keys’ and ‘Qualifiers’ see Appendix II. Viewing amino acid or protein sequence C ...
Visualization, description and analysis of the Drosophila melanogaster
... The mathematical foundations of population genetics were established by R. A. Fisher, J. B. S. Haldane and S. Wright in the second and third decades of the XX century. They figured out the consequences of chance and selection in populations with Mendelian inheritance, and turned population genetics ...
... The mathematical foundations of population genetics were established by R. A. Fisher, J. B. S. Haldane and S. Wright in the second and third decades of the XX century. They figured out the consequences of chance and selection in populations with Mendelian inheritance, and turned population genetics ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.