Purification of Arrestin from Bovine Retinas
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
... It has been reported that bovine arrestin may contain lipids and carbohydrate moieties, covalently attached to the protein (for review, see Ref. 22). It was also reported that arrestin can be modified substoichiometrically by phosphorylation, presumably by protein kinase C (23). Mass spectroscopic a ...
Identification and analysis of new phloem proteins from
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
Dynamics of lipoprotein transport in the human circulatory system
... mutagenesis of key amino acids of receptor- and heparin-binding sites that are absent from pancreatic lipase (Fig. 2). LPL is a serine hydrolase whose active site triad is made up of the $1.~2, Disc, and H24j residues. Consistent with other lipases in this family, the primary sequence of LPL predict ...
... mutagenesis of key amino acids of receptor- and heparin-binding sites that are absent from pancreatic lipase (Fig. 2). LPL is a serine hydrolase whose active site triad is made up of the $1.~2, Disc, and H24j residues. Consistent with other lipases in this family, the primary sequence of LPL predict ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... Cindy Knecht and Andrea Crago from the disability service that was provided by the OSU and the Professional Reporters, Inc. Their amazing real-time transcribing work truly helped me overcome the double barriers of disability and language. Thank to OSU provided such a friendly campus. I thank people ...
... Cindy Knecht and Andrea Crago from the disability service that was provided by the OSU and the Professional Reporters, Inc. Their amazing real-time transcribing work truly helped me overcome the double barriers of disability and language. Thank to OSU provided such a friendly campus. I thank people ...
attached paper highlights
... neonatal cardiomyocytes produces an increase in mitochondrial content that is preceded by enhanced expression of NRF1, indicating a role for this transcription factor in cardiac mitochondrial biogenesis.16 The Tfam promoter contains recognition sites for NRF1 and/or NRF2, thus allowing coordination ...
... neonatal cardiomyocytes produces an increase in mitochondrial content that is preceded by enhanced expression of NRF1, indicating a role for this transcription factor in cardiac mitochondrial biogenesis.16 The Tfam promoter contains recognition sites for NRF1 and/or NRF2, thus allowing coordination ...
Golgins and GTPases, giving identity and structure to the Golgi
... This doesn’t mean that they are unimportant. Rather, it probably indicates that the Golgi is structurally adapted in different species to meet the specific needs of different cell types. For example, the recent finding that GM130 is not required for mammalian cell viability at 34.5 8C, but is essent ...
... This doesn’t mean that they are unimportant. Rather, it probably indicates that the Golgi is structurally adapted in different species to meet the specific needs of different cell types. For example, the recent finding that GM130 is not required for mammalian cell viability at 34.5 8C, but is essent ...
Protein O-linked β-N-acetylglucosamine
... are, a long N100 kDa form, and a short form (~70 kDa) with the OGlcNAcase catalytic regions in the N-terminal domain [6,79]. The Cterminal region of the long isoform has a histone acetyl-transferase like domain with a caspase cleavage site between the two regions suggesting potential regulation in r ...
... are, a long N100 kDa form, and a short form (~70 kDa) with the OGlcNAcase catalytic regions in the N-terminal domain [6,79]. The Cterminal region of the long isoform has a histone acetyl-transferase like domain with a caspase cleavage site between the two regions suggesting potential regulation in r ...
Nicotine Increases Hepatic Oxygen Uptake in the Isolated Perfused
... nicotine, a phenomenon prevented by incubation of cells with d-tubocurarine, a nicotinic acetylcholine receptor antagonist. Respiration was also increased ⬃30% in hepatocytes isolated from fed rats by nicotine, whereas hepatocytes isolated from fasted rats showed little response. In the presence of ...
... nicotine, a phenomenon prevented by incubation of cells with d-tubocurarine, a nicotinic acetylcholine receptor antagonist. Respiration was also increased ⬃30% in hepatocytes isolated from fed rats by nicotine, whereas hepatocytes isolated from fasted rats showed little response. In the presence of ...
THE PHYSICAL NATURE OF F PLASMID TRAI AND
... but there have been many friends and family who have lent their support generously. The first people who I’d like to thank are all the great friends I’ve made in graduate school. Specifically, I want to thank my friend Monica Berrondo, a once fellow graduate student who I met while doing Tae Kwon Do ...
... but there have been many friends and family who have lent their support generously. The first people who I’d like to thank are all the great friends I’ve made in graduate school. Specifically, I want to thank my friend Monica Berrondo, a once fellow graduate student who I met while doing Tae Kwon Do ...
Single-Amino Acid Substitutions Alter the Specificity and Affinity of
... domain of PSD-95 with Arg and expressed both the wildtype (PDZ2) and mutant (K165R) PDZ domains as thioredoxin fusion proteins in bacteria. Both were expressed at high levels and were highly soluble. Their ability to interact with nNOS was assayed by overlaying blots with a fusion protein containing ...
... domain of PSD-95 with Arg and expressed both the wildtype (PDZ2) and mutant (K165R) PDZ domains as thioredoxin fusion proteins in bacteria. Both were expressed at high levels and were highly soluble. Their ability to interact with nNOS was assayed by overlaying blots with a fusion protein containing ...
23 Amino Acids, Peptides, and Proteins
... essential amino acids from our diets because we either cannot synthesize them at all or cannot synthesize them in adequate amounts. For example, we must have a dietary source of phenylalanine because we cannot synthesize benzene rings. However, we do not need tyrosine in our diets, because we can sy ...
... essential amino acids from our diets because we either cannot synthesize them at all or cannot synthesize them in adequate amounts. For example, we must have a dietary source of phenylalanine because we cannot synthesize benzene rings. However, we do not need tyrosine in our diets, because we can sy ...
A E M , Feb. 2004, p. 999–1007
... occupies the end of the predicted substrate-binding pocket and obstructs the binding of straight fatty acid chains longer than four carbons. On the other hand, a Thr residue occupies position 87 in the M. tuberculosis FabH protein, and this enzyme displays specificity for substrates of C8 to C16 len ...
... occupies the end of the predicted substrate-binding pocket and obstructs the binding of straight fatty acid chains longer than four carbons. On the other hand, a Thr residue occupies position 87 in the M. tuberculosis FabH protein, and this enzyme displays specificity for substrates of C8 to C16 len ...
Plant and Soil
... The inactive Fe protein carried phosphate, ribose and an 'adenine-like' unit. With an active Fe protein available it was possible to purify both dinitrogenase and dinitrogenase reductase from R. rubrum, and the properties of both were similar to those of other nitrogenase proteins. The modifying gro ...
... The inactive Fe protein carried phosphate, ribose and an 'adenine-like' unit. With an active Fe protein available it was possible to purify both dinitrogenase and dinitrogenase reductase from R. rubrum, and the properties of both were similar to those of other nitrogenase proteins. The modifying gro ...
Polycystin-1 maturation requires polycystin-2 in a dose
... with PC1-FL and PC1-CTS, as well as PC1-NTS via PC1-CTS/PC1NTS tethering, before PC1 maturation. Since PC2 was not affected by swainsonine and remained sensitive to EndoH, these data suggest that only PC1 passes through the Golgi, while PC2 is mostly retained in the ER. Localization of PC1 glycoform ...
... with PC1-FL and PC1-CTS, as well as PC1-NTS via PC1-CTS/PC1NTS tethering, before PC1 maturation. Since PC2 was not affected by swainsonine and remained sensitive to EndoH, these data suggest that only PC1 passes through the Golgi, while PC2 is mostly retained in the ER. Localization of PC1 glycoform ...
6 PUFA - SENS Research Foundation
... 37C by oxidation of pyruvate (5mM)+ malate (0.5mM) during complete conversion of 0.5mM ADP to ATP ...
... 37C by oxidation of pyruvate (5mM)+ malate (0.5mM) during complete conversion of 0.5mM ADP to ATP ...
Quality control in tRNA charging — editing of homocysteine
... LysRS, ProRS, PheRS, and ThrRS are less accurate and have the ability to misactivate non-cognate amino acids (reaction 1). Misactivation occurs because there is a limited scope for structural variation among related amino acids. Pauling first recognized this problem in 1957, well before the mechanis ...
... LysRS, ProRS, PheRS, and ThrRS are less accurate and have the ability to misactivate non-cognate amino acids (reaction 1). Misactivation occurs because there is a limited scope for structural variation among related amino acids. Pauling first recognized this problem in 1957, well before the mechanis ...
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... mutations of PP1 in fungi could be (partially) complemented by expression of mammalian PP1 (113, 311), indicating that PP1 is also functionally conserved. Eukaryotic genomes contain one (Saccharomyces cerevisiae) to eight genes (Arabidopsis thaliana) encoding PP1 isoforms. More than 70% of the resid ...
... mutations of PP1 in fungi could be (partially) complemented by expression of mammalian PP1 (113, 311), indicating that PP1 is also functionally conserved. Eukaryotic genomes contain one (Saccharomyces cerevisiae) to eight genes (Arabidopsis thaliana) encoding PP1 isoforms. More than 70% of the resid ...
Intragenic Revertants of Yeast Invertase Variants with Secretion-Defective Leader Sequences.
... the secretory pathway (C. A. Kaiser, Ph.D. thesis, Massachusetts Institute of Technology, Cambridge, 1987). Thus, these amino-terminal residues constitute a signal sequence for secretion. They are necessary for invertase secretion, since the constitutive form of invertase remains in the cytoplasm, a ...
... the secretory pathway (C. A. Kaiser, Ph.D. thesis, Massachusetts Institute of Technology, Cambridge, 1987). Thus, these amino-terminal residues constitute a signal sequence for secretion. They are necessary for invertase secretion, since the constitutive form of invertase remains in the cytoplasm, a ...
The mechanism of Stx2 enrichment in outer membrane vesicles of
... In this aim, we will purify OMVs and periplasm from E. coli O157:H7 and use Western blotting to confirm that Stx1 is present at a higher concentration in the OMVs than in the periplasm. To begin the identification of proteins that interact with Stx1 and are present in the OMVs, we will use velocity ...
... In this aim, we will purify OMVs and periplasm from E. coli O157:H7 and use Western blotting to confirm that Stx1 is present at a higher concentration in the OMVs than in the periplasm. To begin the identification of proteins that interact with Stx1 and are present in the OMVs, we will use velocity ...
KINETIC STUDY OF ACAMPROSATE ABSORPTION IN RAT SMALL
... (Received 25 October 1999; in revised form 3 February 2000; accepted 10 February 2000) Abstract — Acamprosate (calcium bis acetyl-homotaurine), a homotaurine derivative, a structural analogue of γ-aminobutyric acid (GABA) and an upper homologue of taurine, is a relatively new drug used to prevent re ...
... (Received 25 October 1999; in revised form 3 February 2000; accepted 10 February 2000) Abstract — Acamprosate (calcium bis acetyl-homotaurine), a homotaurine derivative, a structural analogue of γ-aminobutyric acid (GABA) and an upper homologue of taurine, is a relatively new drug used to prevent re ...
Structure of the ordered hydration of amino acids
... 2013). Zheng et al. (2013) calculated radial distribution functions of water around various protein atom types and calculated the corresponding potentials of mean force (wPMF). This allowed the authors to assign a wPMF score to individual water molecules in protein structures and also to predict pot ...
... 2013). Zheng et al. (2013) calculated radial distribution functions of water around various protein atom types and calculated the corresponding potentials of mean force (wPMF). This allowed the authors to assign a wPMF score to individual water molecules in protein structures and also to predict pot ...
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... follistatin, but harbors only two instead of three follistatin modules (Tortoriello et al., 2001). With respect to activin binding ability, functional homology among follistatin domain-containing proteins is only found between follistatin and FSTL3, whereas all other follistatin family proteins have ...
... follistatin, but harbors only two instead of three follistatin modules (Tortoriello et al., 2001). With respect to activin binding ability, functional homology among follistatin domain-containing proteins is only found between follistatin and FSTL3, whereas all other follistatin family proteins have ...
Molecular Genetic Analysis of Phototropism in
... phototropin1 (phot1) and phototropin2 (phot2), with corresponding gene name changes (Briggs et al. 2001). Blue light irradiation activates the kinase activity of the phototropins (reviewed by Christie 2007). Each FMN molecule associates with the LOV domains non-covalently in a dark state. Blue light ...
... phototropin1 (phot1) and phototropin2 (phot2), with corresponding gene name changes (Briggs et al. 2001). Blue light irradiation activates the kinase activity of the phototropins (reviewed by Christie 2007). Each FMN molecule associates with the LOV domains non-covalently in a dark state. Blue light ...
Enzymes responsible for chlorate reduction by Pseudomonas sp
... strain KJ (Table 2). SDS–PAGE analysis of the chlorate reductase from strain PDA indicated three bands with molecular weights of 60, 48, and 27 kDa (Fig. 2(b)). The first 15 amino acids of the N-terminus of the 60 kDa band (SATDISGAFEYSGGE) did not show similarity to any other previously isolated chl ...
... strain KJ (Table 2). SDS–PAGE analysis of the chlorate reductase from strain PDA indicated three bands with molecular weights of 60, 48, and 27 kDa (Fig. 2(b)). The first 15 amino acids of the N-terminus of the 60 kDa band (SATDISGAFEYSGGE) did not show similarity to any other previously isolated chl ...