Characterization of NAD Salvage Pathways and their Role in
... Rodionov et al., 2008; Sorci et al., 2013; Spector et al., 1985; Zhu et al., 1988). While the de ...
... Rodionov et al., 2008; Sorci et al., 2013; Spector et al., 1985; Zhu et al., 1988). While the de ...
A Raman spectroscopic study of the interaction between nucleotides
... causes no shielding of the SH groups. With Raman difference spectroscopy i t is shown that binding of the protein to a single-stranded nucleotide involves both tyrosine and trytophan residues. A change in the secondary structure of the protein upon binding is observed. In the complex, gp32 contains ...
... causes no shielding of the SH groups. With Raman difference spectroscopy i t is shown that binding of the protein to a single-stranded nucleotide involves both tyrosine and trytophan residues. A change in the secondary structure of the protein upon binding is observed. In the complex, gp32 contains ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... Many AraC family members possess similar solubility problems, and because they most obviously share homologous DNA-binding domains, these domains were expected to be insoluble. It was a surprise, however, to discover that the AraC DNA-binding domain itself is soluble, well behaved biochemically, and ...
... Many AraC family members possess similar solubility problems, and because they most obviously share homologous DNA-binding domains, these domains were expected to be insoluble. It was a surprise, however, to discover that the AraC DNA-binding domain itself is soluble, well behaved biochemically, and ...
PROTEIN PHOSPHORYLATION AND CELLULAR REGULATION, I by
... a was active in the absence of this nucleotide. Since the concentration of 5’-AMP required for the activity of phosphorylase b was considerably higher ’ than that found in muscle, this form was considered to be physiologically inactive. Phosphorylas e a was thought of as the physiologically active s ...
... a was active in the absence of this nucleotide. Since the concentration of 5’-AMP required for the activity of phosphorylase b was considerably higher ’ than that found in muscle, this form was considered to be physiologically inactive. Phosphorylas e a was thought of as the physiologically active s ...
WldS and PGC-1 Regulate Mitochondrial Transport and Oxidation
... Figure 2. Axonal injury causes mitochondrial transport arrest in a separated axon fragment. A, B, Mitochondrial transport was imaged in a 50 m axon segment before (A) and after (B) GFP- and mitoDsRed-expressing axons were severed with a laser (yellow arrow points to injury site). Transport was moni ...
... Figure 2. Axonal injury causes mitochondrial transport arrest in a separated axon fragment. A, B, Mitochondrial transport was imaged in a 50 m axon segment before (A) and after (B) GFP- and mitoDsRed-expressing axons were severed with a laser (yellow arrow points to injury site). Transport was moni ...
METABOLIC CUES AND REGULATORY PROTEINS
... Maris Fonseca, Ari Molofsky, J-D Sauer, Natalie Whitfield and Brian Yagi. Each has provided me with thoughtful suggestions regarding my thesis project and has made the Swanson lab a fun and exciting place to work. Thanks to Dr. Carmen Buchrieser and Dr. Matthieu Jules who enabled me to conduct resea ...
... Maris Fonseca, Ari Molofsky, J-D Sauer, Natalie Whitfield and Brian Yagi. Each has provided me with thoughtful suggestions regarding my thesis project and has made the Swanson lab a fun and exciting place to work. Thanks to Dr. Carmen Buchrieser and Dr. Matthieu Jules who enabled me to conduct resea ...
Protons and how they are transported by proton pumps
... exported ions, whereas in E2 the same site(s) have low affinity for the exported ions and high affinity for the counter-ions being imported. In plasma membrane H+-ATPases, a single proton is believed to be transported per hydrolyzed ATP [22]. There is no evidence that counter-ion transport takes pla ...
... exported ions, whereas in E2 the same site(s) have low affinity for the exported ions and high affinity for the counter-ions being imported. In plasma membrane H+-ATPases, a single proton is believed to be transported per hydrolyzed ATP [22]. There is no evidence that counter-ion transport takes pla ...
Pseudomonas aeruginosa Anaerobic Respiration in Biofilms
... formation and cell viability, we grew biofilms in LBN under anaerobic conditions using a panel of mutant strains designed to test genetically whether NO killed the rhlR mutant bacteria. Figure 3E shows that most of the anaerobic ⌬rhlR biofilm bacteria were dead, consistent with the results described ...
... formation and cell viability, we grew biofilms in LBN under anaerobic conditions using a panel of mutant strains designed to test genetically whether NO killed the rhlR mutant bacteria. Figure 3E shows that most of the anaerobic ⌬rhlR biofilm bacteria were dead, consistent with the results described ...
Molecular Interaction between COP1 and HY5
... degrees of interaction observed were not due to differences in protein expression, Western blot analysis was conducted. All of the LexA-HY5 fusion proteins were expressed at similar levels, except LexA-N77 and LexAN40, which were slightly higher and could account for the slightly stronger interactio ...
... degrees of interaction observed were not due to differences in protein expression, Western blot analysis was conducted. All of the LexA-HY5 fusion proteins were expressed at similar levels, except LexA-N77 and LexAN40, which were slightly higher and could account for the slightly stronger interactio ...
The mitochondrial compartment - Plant Mitochondrial dynamics
... Pcp1p genes are present in Arabidopsis: Mgm1p=members of the Arabidopsis dynamin-like gene family (Hong et al., 2003), the closest being DRP3B, At2g14120; Rbd1p/ Pcp1p=At1g18600. At the time of writing, only DRP3B has been shown to be required for normal mitochondrial morphology (Arimura and Tsutsum ...
... Pcp1p genes are present in Arabidopsis: Mgm1p=members of the Arabidopsis dynamin-like gene family (Hong et al., 2003), the closest being DRP3B, At2g14120; Rbd1p/ Pcp1p=At1g18600. At the time of writing, only DRP3B has been shown to be required for normal mitochondrial morphology (Arimura and Tsutsum ...
Protein Profile Characterization of Donkey Milk
... lipocalin family and has high affinity for a wide range of compounds opening the way to various suppositions about its function. In fact it has been shown that this protein is involved in hydrophobic ligand transport and uptake, enzyme regulation, and the neonatal acquisition of passive immunity, ot ...
... lipocalin family and has high affinity for a wide range of compounds opening the way to various suppositions about its function. In fact it has been shown that this protein is involved in hydrophobic ligand transport and uptake, enzyme regulation, and the neonatal acquisition of passive immunity, ot ...
ELM Database Entry Data Collection - Eukaryotic Linear Motif
... This appears as the ‘Abstract’ on the individual ELM pages (e.g. http://elm.eu.org/elms/elmPages/DEG_SCF_TIR1_1.html#abstract). It should be a biological abstract that reviews cellular systems in which the ELM(s) belonging to this functional site work and includes appropriate references (literature, ...
... This appears as the ‘Abstract’ on the individual ELM pages (e.g. http://elm.eu.org/elms/elmPages/DEG_SCF_TIR1_1.html#abstract). It should be a biological abstract that reviews cellular systems in which the ELM(s) belonging to this functional site work and includes appropriate references (literature, ...
Protein kinase C- modulates mitochondrial function and active Na
... TBHP treatment of the RPTC monolayer. RPTC monolayers reached confluence within 6 days and were treated with TBHP (300 M, 45 min) on day 7 of culture. After TBHP exposure, the monolayer was washed with fresh, warm (37°C) medium and cultured for an additional 4 days. Previously, we have shown that T ...
... TBHP treatment of the RPTC monolayer. RPTC monolayers reached confluence within 6 days and were treated with TBHP (300 M, 45 min) on day 7 of culture. After TBHP exposure, the monolayer was washed with fresh, warm (37°C) medium and cultured for an additional 4 days. Previously, we have shown that T ...
Kinesin-1–syntaphilin coupling mediates activity
... of snph+/+ and snph/ neurons expressing DsRed–mitochondria targeting sequence (Mito) and YFP-Rab7. To elevate cytosolic Ca2+, the neurons were treated briefly (2 min) with 50 mM KCl and 10 µM FPL 64176, an L-type Ca2+ channel agonist. This combined application was shown to raise synaptic Ca2+ to l ...
... of snph+/+ and snph/ neurons expressing DsRed–mitochondria targeting sequence (Mito) and YFP-Rab7. To elevate cytosolic Ca2+, the neurons were treated briefly (2 min) with 50 mM KCl and 10 µM FPL 64176, an L-type Ca2+ channel agonist. This combined application was shown to raise synaptic Ca2+ to l ...
Structure-Based Prediction of Asparagine and Aspartate
... fermentation, downstream-processing, and in vivo can often not be controlled sufficiently. If Asn and Asp residues are involved in antigen recognition, their chemical alteration can lead to severe loss of potency [11–15]. In several cases, these degradation events were reported to hamper long-term m ...
... fermentation, downstream-processing, and in vivo can often not be controlled sufficiently. If Asn and Asp residues are involved in antigen recognition, their chemical alteration can lead to severe loss of potency [11–15]. In several cases, these degradation events were reported to hamper long-term m ...
C H A P
... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
The FTLD risk factor TMEM106B and MAP6 control dendritic
... To investigate the role of TMEM106B in neurons, we took advantage of lentiviral knockdown using two specific shRNAs targeting TMEM106B (shT106b#1 and #2). Cortical neurons were transduced at day 7 in vitro (DIV7) with individual shRNAs for 5 days (DIV7+5). Both shRNAs strongly reduced TMEM106B expre ...
... To investigate the role of TMEM106B in neurons, we took advantage of lentiviral knockdown using two specific shRNAs targeting TMEM106B (shT106b#1 and #2). Cortical neurons were transduced at day 7 in vitro (DIV7) with individual shRNAs for 5 days (DIV7+5). Both shRNAs strongly reduced TMEM106B expre ...
Comparative Sequence Analysis of the Genomic Segment 6 of Four
... reproducible and specific. Also, this method provides the advantage of sequencing a representative population of the specific m R N A rather than dealing with a single m R N A sequence through the cloned cDNA. One artefact observed is premature termination (bands in all sequencing lanes); however, c ...
... reproducible and specific. Also, this method provides the advantage of sequencing a representative population of the specific m R N A rather than dealing with a single m R N A sequence through the cloned cDNA. One artefact observed is premature termination (bands in all sequencing lanes); however, c ...
Uptake of organic nitrogen by plants
... by the prominent role of inorganic N in many arable soils and the dependence of many crop plants on this N source. It was also, naturally, motivated by the abundance of inorganic N fertilizers for agricultural use. After the widely discussed human perturbation of the global carbon (C) cycle, anthrop ...
... by the prominent role of inorganic N in many arable soils and the dependence of many crop plants on this N source. It was also, naturally, motivated by the abundance of inorganic N fertilizers for agricultural use. After the widely discussed human perturbation of the global carbon (C) cycle, anthrop ...
The Utilization by Yeasts of Acids of the Tricarboxylic Acid Cycle
... The contents of the Warburg flask side arm were mixed with those of the main compartment, and a t the same moment 175 pmole of sodium acetate in 0.5 ml. water were added to the pipette flask and the contents we1 mixed. When the rate of acetate oxidation was steady (usually after about 20 min.) the a ...
... The contents of the Warburg flask side arm were mixed with those of the main compartment, and a t the same moment 175 pmole of sodium acetate in 0.5 ml. water were added to the pipette flask and the contents we1 mixed. When the rate of acetate oxidation was steady (usually after about 20 min.) the a ...
Cloning, Sequencing, and Characterization of Luciola italica
... comparisons among firefly species can be used to determine important structure-function similarities and differences among their luciferase enzymes. Amino acids shared between species’ sequences are likely to serve an important role in the catalysis of this reaction and identifying them will lead to ...
... comparisons among firefly species can be used to determine important structure-function similarities and differences among their luciferase enzymes. Amino acids shared between species’ sequences are likely to serve an important role in the catalysis of this reaction and identifying them will lead to ...
Chaperone-dependent gene expression of organic
... 3.7. Phylogenetic analysis of S5 lipase The G + C content of a gene encoding a protein may reveal the origin of the protein. The G + C content was calculated using FramePlot 2.3.2 software available at http://www.watson.nih.go.jp. The G + C content of this organic solvent-tolerant lipase gene was 65 ...
... 3.7. Phylogenetic analysis of S5 lipase The G + C content of a gene encoding a protein may reveal the origin of the protein. The G + C content was calculated using FramePlot 2.3.2 software available at http://www.watson.nih.go.jp. The G + C content of this organic solvent-tolerant lipase gene was 65 ...
Evidence for chloroplast control of external Ca -induced
... ([Ca2+]ext)-induced [Ca2+]cyt transients and stomatal closure in Arabidopsis. CAS (Ca2+ sensing receptor) is a plant-specific putative Ca2+-binding protein that was originally proposed to be a plasma membrane-localized external Ca2+ sensor. In the present study, we characterized the intracellular lo ...
... ([Ca2+]ext)-induced [Ca2+]cyt transients and stomatal closure in Arabidopsis. CAS (Ca2+ sensing receptor) is a plant-specific putative Ca2+-binding protein that was originally proposed to be a plasma membrane-localized external Ca2+ sensor. In the present study, we characterized the intracellular lo ...
Characteristics of Whey Flavor - Innovation Center for US Dairy
... Whey is the liquid substance obtained by separating the coagulum from milk or cream in cheese making. Acid whey is obtained from a process in which either a significant amount of lactose is converted to lactic acid or from curd formation by direct acidification of milk. Sweet whey is obtained from a ...
... Whey is the liquid substance obtained by separating the coagulum from milk or cream in cheese making. Acid whey is obtained from a process in which either a significant amount of lactose is converted to lactic acid or from curd formation by direct acidification of milk. Sweet whey is obtained from a ...