Recycling of vitamin B12 and NAD+ within the Pdu
... Oxidoreductive conversions between the three oxidation states are thus of key importance in the chemistry of vitamin B12. When the oxidation state of the cobalt atom decreases its coordination number tends to decrease accordingly. 1.1.2. Biosynthesis of vitamin B12 According to a widely accepted his ...
... Oxidoreductive conversions between the three oxidation states are thus of key importance in the chemistry of vitamin B12. When the oxidation state of the cobalt atom decreases its coordination number tends to decrease accordingly. 1.1.2. Biosynthesis of vitamin B12 According to a widely accepted his ...
The bicoid Protein Determines Position in the Drosophila Embryo in
... Another line of evidence that the bcd protein is not only distributed in a graded fashion but that this distribution determines its biological function derives from the analysis of embryos from females with increased or decreased bcd gene dosage. For the anterior pattern we demonstrated that the shi ...
... Another line of evidence that the bcd protein is not only distributed in a graded fashion but that this distribution determines its biological function derives from the analysis of embryos from females with increased or decreased bcd gene dosage. For the anterior pattern we demonstrated that the shi ...
Planta
... histidines at the N-terminal end of the protein, allowing a one-step puri®cation of the resulting protein. The resulting vectors were named pQE50iso and pQE30iso. As the peptide sequence preceding the pair of arginines was similar to a plastidial transit peptide (Fig. 1), a sequence lacking this 5¢ ...
... histidines at the N-terminal end of the protein, allowing a one-step puri®cation of the resulting protein. The resulting vectors were named pQE50iso and pQE30iso. As the peptide sequence preceding the pair of arginines was similar to a plastidial transit peptide (Fig. 1), a sequence lacking this 5¢ ...
Appendix 1: PBS listings of Nutritional Products
... Benefits Scheme (PBS). This review focuses on products for cows’ milk protein intolerance following the July 2012 changes to the PBS restrictions for these products. The DUSC requested an analysis of these products at its February 2015 meeting. Date of listing on the Pharmaceutical Benefits Scheme ( ...
... Benefits Scheme (PBS). This review focuses on products for cows’ milk protein intolerance following the July 2012 changes to the PBS restrictions for these products. The DUSC requested an analysis of these products at its February 2015 meeting. Date of listing on the Pharmaceutical Benefits Scheme ( ...
Eucalyptus Arabidopsis grandis thaliana
... The ever-growing demand for wood and wood derived cellulose products coupled with the public concern about the clearance of natural flora or agricultural land to make way for the forest tree plantations has sparked a renewed interest in the genetic improvement of forest tree species. Conventional br ...
... The ever-growing demand for wood and wood derived cellulose products coupled with the public concern about the clearance of natural flora or agricultural land to make way for the forest tree plantations has sparked a renewed interest in the genetic improvement of forest tree species. Conventional br ...
(LPT1) and humans (LPCAT3)
... the human MBOAT5, a protein with unknown function. Cell lysates from insect cells expressing human MBOAT5 (renamed as LPCAT3) showed a 10 fold increase in LPCAT activity over control. In summary, the identification of a novel LPLAT in yeast has proved to be the founding member of LPLAT family in hum ...
... the human MBOAT5, a protein with unknown function. Cell lysates from insect cells expressing human MBOAT5 (renamed as LPCAT3) showed a 10 fold increase in LPCAT activity over control. In summary, the identification of a novel LPLAT in yeast has proved to be the founding member of LPLAT family in hum ...
Mitochondrial translation factors of Trypanosoma brucei: elongation
... GTPase activity of EF-Tu is stimulated, causing a conformational change that eventually leads to the release of the aminoacylated tRNA. The GTP exchange factor EF-Ts binds to the GDP-form of EF-Tu and induces its recycling by exchanging GDP for GTP. The conserved bacterial GTPase EF-G has two functi ...
... GTPase activity of EF-Tu is stimulated, causing a conformational change that eventually leads to the release of the aminoacylated tRNA. The GTP exchange factor EF-Ts binds to the GDP-form of EF-Tu and induces its recycling by exchanging GDP for GTP. The conserved bacterial GTPase EF-G has two functi ...
Geometrical and Sequence Characteristics of
... 20-dimensional amino acid composition space (Chou and Zhang, 1995). Because these two parameters showed identical trends in all calculations, only the values of Hamming distances have been discussed in detail. Change in proportion of an amino acid in a given helix class is considered to be significa ...
... 20-dimensional amino acid composition space (Chou and Zhang, 1995). Because these two parameters showed identical trends in all calculations, only the values of Hamming distances have been discussed in detail. Change in proportion of an amino acid in a given helix class is considered to be significa ...
Mitochondrial Dynamics in Mammals
... Mammals have two homologs of Fzo, termed mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2). These proteins are 80% similar to each other and are broadly expressed in a wide range of cell types (Rojo et al., 2002; Santel et al., 2003). Most studies have described a uniform localization of human Mfn1 and Mfn2 ...
... Mammals have two homologs of Fzo, termed mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2). These proteins are 80% similar to each other and are broadly expressed in a wide range of cell types (Rojo et al., 2002; Santel et al., 2003). Most studies have described a uniform localization of human Mfn1 and Mfn2 ...
embor2011116-sup-0001
... This work exploited the available crystallographic structural details of the amyloid fibril forming hexapeptide NNQQNY, to predict the regions of polypeptide sequences forming amyloid fibrils. The crystal structure of NNQQNY was used to create in silico a collection of near-native templates, or 3D p ...
... This work exploited the available crystallographic structural details of the amyloid fibril forming hexapeptide NNQQNY, to predict the regions of polypeptide sequences forming amyloid fibrils. The crystal structure of NNQQNY was used to create in silico a collection of near-native templates, or 3D p ...
NMR studies of the methionine methyl groups in calmodulin
... [3]. NMR and X-ray studies of complexes of CaM with various CaM-binding domain peptides show that all the Met residues, with the possible exception of Met-76, are in contact with the hydrophobic face(s) of the bound peptides [8-12]. Oxidation studies of CaM's Met residues have revealed that all of t ...
... [3]. NMR and X-ray studies of complexes of CaM with various CaM-binding domain peptides show that all the Met residues, with the possible exception of Met-76, are in contact with the hydrophobic face(s) of the bound peptides [8-12]. Oxidation studies of CaM's Met residues have revealed that all of t ...
REVIEWS - Haseloff Lab
... activates the ubiquitin moiety by forming a high energy thioester bond between an E1 Cys residue and the carboxy-terminal Gly of ubiquitin (see the figure, part a). This activated ubiquitin is then donated to a Cys on an E2 (or ubiquitin-conjugating enzyme) by trans-esterification. In most cases, th ...
... activates the ubiquitin moiety by forming a high energy thioester bond between an E1 Cys residue and the carboxy-terminal Gly of ubiquitin (see the figure, part a). This activated ubiquitin is then donated to a Cys on an E2 (or ubiquitin-conjugating enzyme) by trans-esterification. In most cases, th ...
and Rhizobiales-Like PPP-Family Protein Phosphatases from
... phosphatases from multiple organisms. The primary goal of the research presented here was to elucidate the evolutionary, biochemical, cellular and biological characteristics of two recently identified PPP-family protein phosphatase subclasses from the model photosynthetic Eukaryote Arabidopsis thali ...
... phosphatases from multiple organisms. The primary goal of the research presented here was to elucidate the evolutionary, biochemical, cellular and biological characteristics of two recently identified PPP-family protein phosphatase subclasses from the model photosynthetic Eukaryote Arabidopsis thali ...
Predicting DNA-binding sites of proteins from amino acid sequence
... contacts that mediate protein-DNA recognition [4-6]. In addition to biophysical methods for structure determination, biochemical and molecular genetic approaches have been widely used to identify DNA-binding sites on proteins and to investigate the interaction modes between proteins and DNA. For exa ...
... contacts that mediate protein-DNA recognition [4-6]. In addition to biophysical methods for structure determination, biochemical and molecular genetic approaches have been widely used to identify DNA-binding sites on proteins and to investigate the interaction modes between proteins and DNA. For exa ...
Ornithodoros savignyi CHAPTER 2 SIGNALING PATHWAYS REGULATING PROTEIN SECRETION FROM
... SDS-PAGE analysis of the secreted proteins showed variable results in response to different agonists. The highest number of proteins was detected from ticks in the earliest stages of feeding. In some ticks, proteins were identified at one collection time but not at another time in secretions from th ...
... SDS-PAGE analysis of the secreted proteins showed variable results in response to different agonists. The highest number of proteins was detected from ticks in the earliest stages of feeding. In some ticks, proteins were identified at one collection time but not at another time in secretions from th ...
Identification of a Chloroplast-encoded 9-kDa
... from spinach and showed that this polypeptide contains approximately 8 cysteine residues. Similar results pointing toward the 9-kDa polypeptide as an iron-sulfur protein of PS-I have been obtained in labeling studies with Anacystis nidulans (18). In contrast to these two studies, Bonnerjea et al. (1 ...
... from spinach and showed that this polypeptide contains approximately 8 cysteine residues. Similar results pointing toward the 9-kDa polypeptide as an iron-sulfur protein of PS-I have been obtained in labeling studies with Anacystis nidulans (18). In contrast to these two studies, Bonnerjea et al. (1 ...
Cyclic-‐di-‐AMP: another second messenger enters the fray
... regulator15) from B. subtilis physically interacts with DacA and stimulates cyclase activity15. The structures of two of the four YbbR domain-‐containing proteins from Desulfitobacterium hafniense Y51 hav ...
... regulator15) from B. subtilis physically interacts with DacA and stimulates cyclase activity15. The structures of two of the four YbbR domain-‐containing proteins from Desulfitobacterium hafniense Y51 hav ...
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase and
... in tryptophan metabolism in an attempt to maintain NAD+ levels. These results suggest a connection between ceramide and NAD metabolism. One of the main NAD+-consuming pathways involves sirtuins because they are NAD+-dependent enzymes, and the availability of NAD+ is an important mechanism that regul ...
... in tryptophan metabolism in an attempt to maintain NAD+ levels. These results suggest a connection between ceramide and NAD metabolism. One of the main NAD+-consuming pathways involves sirtuins because they are NAD+-dependent enzymes, and the availability of NAD+ is an important mechanism that regul ...
Outer Membrane Vesicle-Mediated Export of
... and PKD2) has not yet been fully investigated, although they have been suggested to be involved in protein-protein or protein-carbohydrate interaction [12]. Our previous studies provided a crystal structure model of the PKD1 domain from V. cholerae PrtV (residues 755– 838) and revealed a Ca++-bindin ...
... and PKD2) has not yet been fully investigated, although they have been suggested to be involved in protein-protein or protein-carbohydrate interaction [12]. Our previous studies provided a crystal structure model of the PKD1 domain from V. cholerae PrtV (residues 755– 838) and revealed a Ca++-bindin ...
Soybean Meal – An Exceptional Protein Source
... Soybean meal has long been considered an outstanding source of supplemental protein in diets for livestock and poultry. In fact, soybean meal is sometimes referred to as the "gold standard" because other protein sources are often compared to it. Soybean meal is rich in highly digestible protein, and ...
... Soybean meal has long been considered an outstanding source of supplemental protein in diets for livestock and poultry. In fact, soybean meal is sometimes referred to as the "gold standard" because other protein sources are often compared to it. Soybean meal is rich in highly digestible protein, and ...
The Multiple Personalities of the Regulatory Subunit of Protein
... As the regulatory subunit of a protein kinase that has functions associated with cell cycle progression, CK2β is reminiscent of cyclins that are the regulatory subunits of cyclin-dependent kinases. In a related respect, it is particularly intriguing that CK2β has motifs that have been previously cha ...
... As the regulatory subunit of a protein kinase that has functions associated with cell cycle progression, CK2β is reminiscent of cyclins that are the regulatory subunits of cyclin-dependent kinases. In a related respect, it is particularly intriguing that CK2β has motifs that have been previously cha ...
Structure, mechanism and function of prenyltransferases
... Over the past decade, many trans-IPPSs have been purified and their genes cloned [45,46]. The deduced amino-acid sequences of these enzymes show amino-acid sequence homology and two common DDxxD motifs [47], suggesting that they evolved from the same origin (Fig. 2) [48,49]. These Asp-rich motifs wer ...
... Over the past decade, many trans-IPPSs have been purified and their genes cloned [45,46]. The deduced amino-acid sequences of these enzymes show amino-acid sequence homology and two common DDxxD motifs [47], suggesting that they evolved from the same origin (Fig. 2) [48,49]. These Asp-rich motifs wer ...