THE RENAL ELECTROGENIC Na+:HCO3 COTRANSPORTER
... predicted Vm would be either more negative or more positive than the initial Vm, depending on whether the net direction of transport was inward or outward, respectively. However, the application of CO2/HCO3− can cause non-specific Vm changes. In the first place, CO2 diffuses rapidly across most cell ...
... predicted Vm would be either more negative or more positive than the initial Vm, depending on whether the net direction of transport was inward or outward, respectively. However, the application of CO2/HCO3− can cause non-specific Vm changes. In the first place, CO2 diffuses rapidly across most cell ...
nitrogen fixation and its improvement through genetic engineering
... experimental approaches were carried out due to the complexity of the nature of the biological nitrogen fixation process. This review summarizes some achievements in revealing the biochemical mechanism of nitrogenase, discovery of non conventional nitrogenase in Streptomyces thermoautotrophicus, ini ...
... experimental approaches were carried out due to the complexity of the nature of the biological nitrogen fixation process. This review summarizes some achievements in revealing the biochemical mechanism of nitrogenase, discovery of non conventional nitrogenase in Streptomyces thermoautotrophicus, ini ...
Case Study: BPTI
... the lengths and the conformation of the N and C-termini, most apparent in the case of the C-terminus from the snake venom protein, which is significantly elongated. This structural similarity becomes clearer after aligning all four protein structures using multiple sequence alignment. The so aligned ...
... the lengths and the conformation of the N and C-termini, most apparent in the case of the C-terminus from the snake venom protein, which is significantly elongated. This structural similarity becomes clearer after aligning all four protein structures using multiple sequence alignment. The so aligned ...
Actin Filaments Play a Critical Role in Vacuolar
... Protoplasts transformed with Spo:GFP or AALP:GFP were incubated with Lat B, and the localization of the GFP signal was examined at various time points after transformation. In the absence of Lat B, both reporter proteins were targeted to the central vacuole (Figure 2A, panels a, f, and k). We also o ...
... Protoplasts transformed with Spo:GFP or AALP:GFP were incubated with Lat B, and the localization of the GFP signal was examined at various time points after transformation. In the absence of Lat B, both reporter proteins were targeted to the central vacuole (Figure 2A, panels a, f, and k). We also o ...
Worm integumentary l-histidine transport
... isotope to a minimum, were made from the same salts used to prepare incubation media. Animals were incubated in isotopically labelled incubation medium at 22 °C for predetermined time intervals ranging from 5 to 30 min. Various calcium or sodium conditions were obtained by replacing calcium or sodiu ...
... isotope to a minimum, were made from the same salts used to prepare incubation media. Animals were incubated in isotopically labelled incubation medium at 22 °C for predetermined time intervals ranging from 5 to 30 min. Various calcium or sodium conditions were obtained by replacing calcium or sodiu ...
catalogue 2012 - olimp
... the leading world experts in the area of sport nutrition with whom we cooperate on a regular basis and the products themselves, put us at the top of the most innovative companies implementing the state of the art and most effective solutions in sport nutrition. Over 20 years of market presence and t ...
... the leading world experts in the area of sport nutrition with whom we cooperate on a regular basis and the products themselves, put us at the top of the most innovative companies implementing the state of the art and most effective solutions in sport nutrition. Over 20 years of market presence and t ...
Polyphosphates of the Yeast Cell Vacuole
... the 2000 g supernatant fluid fraction (Boulton, 1965) this explanation seems improbable. Weimberg & Orton (1965), however, have suggested that part (about 30 %) of the polyphosphate of the yeast cell may be associated with the cell surface. A further possibility is that the polyphosphates are contai ...
... the 2000 g supernatant fluid fraction (Boulton, 1965) this explanation seems improbable. Weimberg & Orton (1965), however, have suggested that part (about 30 %) of the polyphosphate of the yeast cell may be associated with the cell surface. A further possibility is that the polyphosphates are contai ...
Glycoprotein IIIa Is Phosphorylated in Intact Human
... The glycoprotein Ilb-llla complex (GP Ilb-llla) is a multifunctional transmembrane protein on platelets. Its most completely described function is as a fibrinogen receptor that mediates platelet aggregation, but it is also involved in clot retraction, signal transduction, calcium transport, and othe ...
... The glycoprotein Ilb-llla complex (GP Ilb-llla) is a multifunctional transmembrane protein on platelets. Its most completely described function is as a fibrinogen receptor that mediates platelet aggregation, but it is also involved in clot retraction, signal transduction, calcium transport, and othe ...
Full-Text PDF
... 1.1. Sulfate Reducers: Phylogenetic Affiliation and Their Contributory Roles in Industrial Problems Sulfate reducers are ubiquitous anaerobic microorganisms that use sulfate as an electron acceptor to oxidize hydrogen and organic compounds (e.g., lactate, acetate, malate, butyrate) [10] (Table 1). T ...
... 1.1. Sulfate Reducers: Phylogenetic Affiliation and Their Contributory Roles in Industrial Problems Sulfate reducers are ubiquitous anaerobic microorganisms that use sulfate as an electron acceptor to oxidize hydrogen and organic compounds (e.g., lactate, acetate, malate, butyrate) [10] (Table 1). T ...
GTPase domains ofras p21 oncogene protein and elongation factor
... p21 and EF-Tu, is a strong selective pressure related to the proper functioning of the GTPase conformational switch. Overall Strutral Differences. In spite of the striking similarity of the Cc trace of the central (3-sheet, there are several differences in the interaction with the surrounding helice ...
... p21 and EF-Tu, is a strong selective pressure related to the proper functioning of the GTPase conformational switch. Overall Strutral Differences. In spite of the striking similarity of the Cc trace of the central (3-sheet, there are several differences in the interaction with the surrounding helice ...
RNA helicase DDX19 stabilizes ribosomal elongation and
... in vivo. Furthermore, Dbp5 has been shown to be required for Mex67 dissociation (NXF1 in human) from exported mRNA (17), and the nuclear export factor RBM15 was shown to facilitate recognition of NXF1-mRNP complexes by DDX19 in humans (18). Several studies provided evidence that Dbp5 and its homolog ...
... in vivo. Furthermore, Dbp5 has been shown to be required for Mex67 dissociation (NXF1 in human) from exported mRNA (17), and the nuclear export factor RBM15 was shown to facilitate recognition of NXF1-mRNP complexes by DDX19 in humans (18). Several studies provided evidence that Dbp5 and its homolog ...
A Metabolic Node in Action: Chorismate
... a discrete allosteric domain, and that inhibitors act competitively at the catalytic site of different family members which exhibit individuality in the range and extent of molecules recognized as substrate or inhibitor.52 The mutase activity of the T-protein (CM-T domain) is very similar to that of ...
... a discrete allosteric domain, and that inhibitors act competitively at the catalytic site of different family members which exhibit individuality in the range and extent of molecules recognized as substrate or inhibitor.52 The mutase activity of the T-protein (CM-T domain) is very similar to that of ...
A Trafficking Pathway for Anthocyanins Overlaps
... of anthocyanins, we show here that in Arabidopsis (Arabidopsis thaliana), one route for anthocyanin transport to the vacuole involves vesicle-like structures shared with components of the secretory pathway. By colocalizing the red fluorescence of the anthocyanins with green fluorescent protein marke ...
... of anthocyanins, we show here that in Arabidopsis (Arabidopsis thaliana), one route for anthocyanin transport to the vacuole involves vesicle-like structures shared with components of the secretory pathway. By colocalizing the red fluorescence of the anthocyanins with green fluorescent protein marke ...
Лекция 2. Структура и функция белка
... bases. Knowledge of the acid-base properties of amino acids is extremely important in understanding the physical and biological properties of proteins. Moreover, the technology of separating, identifying, and quantifying the different amino acids, which are necessary steps in determining the amino a ...
... bases. Knowledge of the acid-base properties of amino acids is extremely important in understanding the physical and biological properties of proteins. Moreover, the technology of separating, identifying, and quantifying the different amino acids, which are necessary steps in determining the amino a ...
Predicting the sidechain dihedral angle distributions
... structures8 to fix the bond lengths, bond angles, and x backbone dihedral angles of the dipeptide mimetics for our calculations. The higher-resolution set limits the refinement bias on the bond lengths, bond angles, and x dihedral angles associated with the crystal structure determination, but there ...
... structures8 to fix the bond lengths, bond angles, and x backbone dihedral angles of the dipeptide mimetics for our calculations. The higher-resolution set limits the refinement bias on the bond lengths, bond angles, and x dihedral angles associated with the crystal structure determination, but there ...
3 Amino Acids - Minificciones
... groups include alcohols, thiols, thioethers, carboxylic acids, carboxamides, and a variety of basic groups. Most of these groups are chemically reactive. All proteins in all species—bacterial, archaeal, and eukaryotic—are constructed from the same set of 20 amino acids with only a few exceptions. Th ...
... groups include alcohols, thiols, thioethers, carboxylic acids, carboxamides, and a variety of basic groups. Most of these groups are chemically reactive. All proteins in all species—bacterial, archaeal, and eukaryotic—are constructed from the same set of 20 amino acids with only a few exceptions. Th ...
PDF - Wiley Online Library
... total of five proteins (Young, 1992). The S gene has a dual-start motif that allows for translation initiation events at two sites defined by codons 1 and 3, giving rise to two proteins with opposite functions, the antiholin (S107) and the holin (S105) (Bläsi et al., 1989, 1990) The R gene encodes ...
... total of five proteins (Young, 1992). The S gene has a dual-start motif that allows for translation initiation events at two sites defined by codons 1 and 3, giving rise to two proteins with opposite functions, the antiholin (S107) and the holin (S105) (Bläsi et al., 1989, 1990) The R gene encodes ...
The role of AMP-activated protein kinase in the coordination of
... (AMPK-␣) and two regulatory subunits (AMPK- and AMPK-␥) (Fig. 1). In humans, AMPK subunits are encoded by seven genes (␣1, ␣2; 1, 2; ␥1, ␥2, ␥3) that can form at least 12 ␣␥ heterotrimers, thus increasing the diversification of its functions (17, 109, 117). The most studied of these subunits is ...
... (AMPK-␣) and two regulatory subunits (AMPK- and AMPK-␥) (Fig. 1). In humans, AMPK subunits are encoded by seven genes (␣1, ␣2; 1, 2; ␥1, ␥2, ␥3) that can form at least 12 ␣␥ heterotrimers, thus increasing the diversification of its functions (17, 109, 117). The most studied of these subunits is ...
Resistance of Helicoverpa armigera to Cry1Ac toxin from Bacillus
... India and elsewhere. The general argument in not planting refugia is the wide availability of different non-transgenic host plants and other alternate host crops in the immediate vicinity of cotton fields [11]. India also has the distinction of growing large areas of cotton with “illegal” B. thuring ...
... India and elsewhere. The general argument in not planting refugia is the wide availability of different non-transgenic host plants and other alternate host crops in the immediate vicinity of cotton fields [11]. India also has the distinction of growing large areas of cotton with “illegal” B. thuring ...
The Miraculous Benefits of Non Denatured Whey Protein Powder
... muscle tissue. Approximately one third of muscle tissue is composed of branched chain amino acids. The body requires higher amounts of branched chain amino acids during and following exercise, as they are taken up directly by the skeletal muscles, versus first being metabolized through the liver, li ...
... muscle tissue. Approximately one third of muscle tissue is composed of branched chain amino acids. The body requires higher amounts of branched chain amino acids during and following exercise, as they are taken up directly by the skeletal muscles, versus first being metabolized through the liver, li ...
Neurotransmitter Transporters in the Central Nervous System
... cellular loops and the presence of an additional N-glycosylation site in the fourth extracellular loop (Fig. 2). The four transporter-like proteins have significant homology (;20 –30%) with the “classical” members such as the DA, serotonin, GABA, or glycine transporters. However, the overall percent ...
... cellular loops and the presence of an additional N-glycosylation site in the fourth extracellular loop (Fig. 2). The four transporter-like proteins have significant homology (;20 –30%) with the “classical” members such as the DA, serotonin, GABA, or glycine transporters. However, the overall percent ...
HEMAGGLUTINATION BY PURIFIED TYPE I ESCHERICHIA
... Gel Electrophoresis. Sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) was carried out in 12.5% polyacrylamide at a constant current of 25 mA using the method of Laemmli (13). 20-~d samples were l~oiled for 2 rain in a mixture of 10/~1 2% SDS, 10% glycerol, 10 mM TrisHC1, and 1% 2-mer ...
... Gel Electrophoresis. Sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) was carried out in 12.5% polyacrylamide at a constant current of 25 mA using the method of Laemmli (13). 20-~d samples were l~oiled for 2 rain in a mixture of 10/~1 2% SDS, 10% glycerol, 10 mM TrisHC1, and 1% 2-mer ...
Pseudouridine at position 55 in tRNA controls the contents of other
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
Ana Maria da Silva Esteves Dissertation presented to obtain
... membranar DIPPS domain was deemed highly relevant and challenging. The structure of the recombinant bifunctional protein IPCT/DIPPS of Archaeoglobus fulgidus with and without bound CDP-inositol was solved by X-ray crystallography to a resolution of 2.5 and 2.65 Å, respectively. The DIPPS domain comp ...
... membranar DIPPS domain was deemed highly relevant and challenging. The structure of the recombinant bifunctional protein IPCT/DIPPS of Archaeoglobus fulgidus with and without bound CDP-inositol was solved by X-ray crystallography to a resolution of 2.5 and 2.65 Å, respectively. The DIPPS domain comp ...