Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
... C. jejuni ancestry. We characterized a C. coli strain (76339) with four novel multilocus sequence type alleles (ST-5088) and having the capability to express gamma-glutamyltranspeptidase (GGT); an accessory feature in C. jejuni. Our aim was to further characterize unintrogressed C. coli clades 2 and ...
... C. jejuni ancestry. We characterized a C. coli strain (76339) with four novel multilocus sequence type alleles (ST-5088) and having the capability to express gamma-glutamyltranspeptidase (GGT); an accessory feature in C. jejuni. Our aim was to further characterize unintrogressed C. coli clades 2 and ...
Stahl_3rd_ch04_Part2..
... the loss of this rich downstream chemical tour de force. Thus, agonists that restore this natural action would be potentially useful in states where reduced signal transduction leads to undesirable symptoms. There are two major ways to stimulate G protein-linked receptors with full agonist action. F ...
... the loss of this rich downstream chemical tour de force. Thus, agonists that restore this natural action would be potentially useful in states where reduced signal transduction leads to undesirable symptoms. There are two major ways to stimulate G protein-linked receptors with full agonist action. F ...
General introduction - University of Amsterdam
... from a nutrient-rich environment like the body is not necessary (48). Yeasts as model organisms Since ancient times the yeast Saccharomyces cerevisiae has been used for baking and brewing, but in the last century it was recognized as a suitable unicellular model organism to study eukaryotic cells, p ...
... from a nutrient-rich environment like the body is not necessary (48). Yeasts as model organisms Since ancient times the yeast Saccharomyces cerevisiae has been used for baking and brewing, but in the last century it was recognized as a suitable unicellular model organism to study eukaryotic cells, p ...
Mechanisms of transport through the Golgi complex
... Golgi units are made up of tubular networks (Kepes et al., 2005; Matsuura-Tokita et al., 2006; Rambourg et al., 2001). Given the high conservation from yeast to humans of proteins that regulate transport through the Golgi complex (Behnia and Munro, 2005; Lee et al., 2004), it is likely that tubular ...
... Golgi units are made up of tubular networks (Kepes et al., 2005; Matsuura-Tokita et al., 2006; Rambourg et al., 2001). Given the high conservation from yeast to humans of proteins that regulate transport through the Golgi complex (Behnia and Munro, 2005; Lee et al., 2004), it is likely that tubular ...
Trade-offs between tRNA abundance and mRNA secondary
... tial reason for this difference is the G/C content for each genome. Analysis of all coding regions revealed G/C content of 40.1% for S. cerevisiae and 52.6% for E. coli. As G/C residues have a greater chance of forming secondary structures, the reduced number of highly structured regions in S. cerev ...
... tial reason for this difference is the G/C content for each genome. Analysis of all coding regions revealed G/C content of 40.1% for S. cerevisiae and 52.6% for E. coli. As G/C residues have a greater chance of forming secondary structures, the reduced number of highly structured regions in S. cerev ...
Serine/threonine/tyrosine phosphorylation regulates
... STKs, Stk1 and Stk2, both implicated in regulating virulence and antibiotic resistance (Ohlsen & Donat, 2010; Tamber et al., 2010). Recently, it was established that the S. aureus Stk1 phosphorylates and regulates the activity of the global TR MgrA. MgrA belongs to the SarA/MgrA family of TRs that c ...
... STKs, Stk1 and Stk2, both implicated in regulating virulence and antibiotic resistance (Ohlsen & Donat, 2010; Tamber et al., 2010). Recently, it was established that the S. aureus Stk1 phosphorylates and regulates the activity of the global TR MgrA. MgrA belongs to the SarA/MgrA family of TRs that c ...
The Cleavage and Polyadenylation Specificity Factor in Xenopus
... During early development, specific mRNAs receive poly(A) in the cytoplasm. This cytoplasmic polyadenylation reaction correlates with, and in some cases causes, translational stimulation. Previously, it was suggested that a factor similar to the multisubunit nuclear cleavage and polyadenylation speci ...
... During early development, specific mRNAs receive poly(A) in the cytoplasm. This cytoplasmic polyadenylation reaction correlates with, and in some cases causes, translational stimulation. Previously, it was suggested that a factor similar to the multisubunit nuclear cleavage and polyadenylation speci ...
The influence of macrolide antibiotics on the uptake of organic
... were raised in rabbits against human OATP1B1 and OATP1B3, respectively. Both were kind gifts of Professor Dr. D. Keppler (German Cancer Research Center, Heidelberg, Germany). The horseradish peroxidase-conjugated goat anti-rabbit IgG was obtained from Amersham (GE Healthcare Europe GmbH, Munich, Ger ...
... were raised in rabbits against human OATP1B1 and OATP1B3, respectively. Both were kind gifts of Professor Dr. D. Keppler (German Cancer Research Center, Heidelberg, Germany). The horseradish peroxidase-conjugated goat anti-rabbit IgG was obtained from Amersham (GE Healthcare Europe GmbH, Munich, Ger ...
How do potentials derived from structural databases relate to true
... After all, the native proteins may not have been maximally optimized for stability through evolution because itis known that sometimes mutations introduced in the native proteins can yield more stable proteins (Lim et al., 1994). The unique stable fold of a protein is apparently an essential feature ...
... After all, the native proteins may not have been maximally optimized for stability through evolution because itis known that sometimes mutations introduced in the native proteins can yield more stable proteins (Lim et al., 1994). The unique stable fold of a protein is apparently an essential feature ...
Mitochondrial Dynamics
... localizes to the inner surface of the cell membrane at division sites, where it forms a ring structure (Z ring) that enables constriction and scission of the parent into two daughter cells. FtsZ is a GTPase, which can hydrolyze guanosine triphosphate to provide a source of energy. However, it is tho ...
... localizes to the inner surface of the cell membrane at division sites, where it forms a ring structure (Z ring) that enables constriction and scission of the parent into two daughter cells. FtsZ is a GTPase, which can hydrolyze guanosine triphosphate to provide a source of energy. However, it is tho ...
Temperature, pressure, and electrochemical
... Abstract. Thermodynamic calculations can be used to quantify environmental constraints on the speciation of proteins, such as the pH and temperature dependence of ionization state, and the relative chemical stabilities of proteins in different biogeochemical settings. These calculations depend in pa ...
... Abstract. Thermodynamic calculations can be used to quantify environmental constraints on the speciation of proteins, such as the pH and temperature dependence of ionization state, and the relative chemical stabilities of proteins in different biogeochemical settings. These calculations depend in pa ...
Investigating The Metal Binding Sites In Znta, A Zinc Transporting
... Sensitivity to metal salts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60 ...
... Sensitivity to metal salts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60 ...
Nutrient production by symbiotic bacteria
... phenylalanine, 2.9 mmol l−1 tryptophan, 2.7 mmol l−1 cysteine, 1.2 mmol l−1 glycine and 0.6 mmol l−1 tyrosine). Two types of dietary modification were used: addition of the antibiotic rifampicin at 50 µg ml−1 (Rahbé et al., 1994) to the complete diet; and individual omission of each of the 20 amino ...
... phenylalanine, 2.9 mmol l−1 tryptophan, 2.7 mmol l−1 cysteine, 1.2 mmol l−1 glycine and 0.6 mmol l−1 tyrosine). Two types of dietary modification were used: addition of the antibiotic rifampicin at 50 µg ml−1 (Rahbé et al., 1994) to the complete diet; and individual omission of each of the 20 amino ...
Conservation of PHO pathway in ascomycetes and the role of Pho84
... Pho90 is required for this vacuolar degradation (Ghillebert et al. 2011). It appears that yeast cells use two distinct mechanisms for regulation of low-affinity transporters. This degradation of low-affinity transporters, upon phosphate scarcity, serves as a positive feedback loop for upregulation o ...
... Pho90 is required for this vacuolar degradation (Ghillebert et al. 2011). It appears that yeast cells use two distinct mechanisms for regulation of low-affinity transporters. This degradation of low-affinity transporters, upon phosphate scarcity, serves as a positive feedback loop for upregulation o ...
The use of deuteration for the structural study of larger proteins
... • Out-and-back triple-resonance experiments: in pairs: 3D HNCA/ HN(CO)CA 3D HN(CA)CB/ HN(COCA)CB e.g. HN → N → CO → CA → CB (t1) → CA → CO → N → HN 3D HN(CA)CO/ HNCO 3D intra-HN(CA)CO/ HNCO 3D intra-HNCA/ DQ-HNCA further: 4D HN(COCA)NH 3D HN(CACB)CG • Increased resolution using 4D approach: HNCOCA/ ...
... • Out-and-back triple-resonance experiments: in pairs: 3D HNCA/ HN(CO)CA 3D HN(CA)CB/ HN(COCA)CB e.g. HN → N → CO → CA → CB (t1) → CA → CO → N → HN 3D HN(CA)CO/ HNCO 3D intra-HN(CA)CO/ HNCO 3D intra-HNCA/ DQ-HNCA further: 4D HN(COCA)NH 3D HN(CACB)CG • Increased resolution using 4D approach: HNCOCA/ ...
Metabolic significance of inorganic triphosphate, thiamine
... inorganic triphosphate (PPPi) which raises the question of the physiological significance of this compound. We first studied the tripolyphosphatase activity in mammals and in bacteria and we showed that it is widely distributed in all organisms. We attempted to identify the enzymes responsible for t ...
... inorganic triphosphate (PPPi) which raises the question of the physiological significance of this compound. We first studied the tripolyphosphatase activity in mammals and in bacteria and we showed that it is widely distributed in all organisms. We attempted to identify the enzymes responsible for t ...
Evolution of Brachyury proteins: identification of a novel regulatory
... The surprising diversity of the roles of Brachyury in metazoans, and the availability of full-length cDNAs for orthologues from all major phyla, prompted us to test whether these proteins have intrinsically different activities, or whether their different functions reflect changes in their cellular ...
... The surprising diversity of the roles of Brachyury in metazoans, and the availability of full-length cDNAs for orthologues from all major phyla, prompted us to test whether these proteins have intrinsically different activities, or whether their different functions reflect changes in their cellular ...
Symbiotic bacteria enable insect to use a nutritionally inadequate diet
... A. pisum clone LL01 derived originally from an alfalfa crop in France was maintained under the same conditions on threeto four-week-old pre-flowering plants and on chemically defined diets of formulation A (Prosser & Douglas 1992) containing 0.15 M amino acids and 0.5 M sucrose. The diets contained ...
... A. pisum clone LL01 derived originally from an alfalfa crop in France was maintained under the same conditions on threeto four-week-old pre-flowering plants and on chemically defined diets of formulation A (Prosser & Douglas 1992) containing 0.15 M amino acids and 0.5 M sucrose. The diets contained ...
Identification and Analysis of Dicer Associated Proteins in
... 4.2. RNase III enzymes and their dsRNA-binding partners.......................................................... 67 4.3. RNAi and innate immunity: Is there a link in mammals?....................................................... 71 4.4. Ubiquitin as a regulatory component of RNA silencing? ....... ...
... 4.2. RNase III enzymes and their dsRNA-binding partners.......................................................... 67 4.3. RNAi and innate immunity: Is there a link in mammals?....................................................... 71 4.4. Ubiquitin as a regulatory component of RNA silencing? ....... ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... was found to be 1:10,000 (Fig. 3, lane 1). At this dilution, a strong, defined, and specific band of 71 kDa (determined by comparison with comigrating size markers) was observed. Lower dilutions of the primary antibody (1:3,000 and 1:6,000) resulted in a stronger signal, but at the same time produce ...
... was found to be 1:10,000 (Fig. 3, lane 1). At this dilution, a strong, defined, and specific band of 71 kDa (determined by comparison with comigrating size markers) was observed. Lower dilutions of the primary antibody (1:3,000 and 1:6,000) resulted in a stronger signal, but at the same time produce ...
Vanadium-Binding Proteins (Vanabins)
... vanadium-associated proteins (VAPs), in the blood cells of Ascidia sydneiensis samea [9]. These vanabins were identified by a combination of anion exchange chromatography and atomic absorption spectrometry. They include at least two major proteins with apparent molecular weights of 12.5 and 15 kDa, ...
... vanadium-associated proteins (VAPs), in the blood cells of Ascidia sydneiensis samea [9]. These vanabins were identified by a combination of anion exchange chromatography and atomic absorption spectrometry. They include at least two major proteins with apparent molecular weights of 12.5 and 15 kDa, ...
Molecular Cloning and Expression Analysis of IgD in Nile
... Abstract: IgD is considered to be a recently-evolved Ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. Although IgD likely plays an important role in vertebrate immune responses, the function of IgD in Nile tilapia (Oreochromis niloticus) is virtually unkno ...
... Abstract: IgD is considered to be a recently-evolved Ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. Although IgD likely plays an important role in vertebrate immune responses, the function of IgD in Nile tilapia (Oreochromis niloticus) is virtually unkno ...