from dicp.ac.cn
... five sites are located in the MSK1 C-terminus, close to Thr700, which is conserved in MAPK-integrating kinase 1 and 2 (MNK1 and MNK2) (6). According to a recent activation model of MSK1, phosphorylation of Thr700 relieves inhibition of MSK1 by a C-terminal autoinhibitory helix, and helps induce a co ...
... five sites are located in the MSK1 C-terminus, close to Thr700, which is conserved in MAPK-integrating kinase 1 and 2 (MNK1 and MNK2) (6). According to a recent activation model of MSK1, phosphorylation of Thr700 relieves inhibition of MSK1 by a C-terminal autoinhibitory helix, and helps induce a co ...
Influenza virus assembly and budding
... in eggs causes a loss of the filamentous morphology (Choppin et al., 1960). Indeed, the two most widely used laboratory spherical strains, A/WSN/33 and A/PR/8, have been passaged extensively in eggs. Thus, the loss of filament-forming ability may be an adaptation to growth in eggs and not specifically ...
... in eggs causes a loss of the filamentous morphology (Choppin et al., 1960). Indeed, the two most widely used laboratory spherical strains, A/WSN/33 and A/PR/8, have been passaged extensively in eggs. Thus, the loss of filament-forming ability may be an adaptation to growth in eggs and not specifically ...
Probing for Binding Regions of the FtsZ Protein Surface through Site
... expressed at levels lower than that of wild-type FtsZ (wtFtsZ). However, neither fusion could function as the sole source of FtsZ. This suggested that these FtsZ fusions could copolymerize and therefore localize midcell with wtFtsZ, but the fusions apparently interfered with some functions when the ...
... expressed at levels lower than that of wild-type FtsZ (wtFtsZ). However, neither fusion could function as the sole source of FtsZ. This suggested that these FtsZ fusions could copolymerize and therefore localize midcell with wtFtsZ, but the fusions apparently interfered with some functions when the ...
Designing logical codon reassignment
... the advantage of being applicable to the study of intracellular biological processes in the very cellular system that produced them. To date, over 150 different UAAs have been incorporated into proteins. These have been used as tools for a number of applications including the study of protein–protein ...
... the advantage of being applicable to the study of intracellular biological processes in the very cellular system that produced them. To date, over 150 different UAAs have been incorporated into proteins. These have been used as tools for a number of applications including the study of protein–protein ...
View Full PDF - Essays in Biochemistry
... very few enzymes that uses pyruvate, instead of pyridoxal 5′-phosphate, as a cofactor [18]. The pyruvate is generated by an autocatalytical cleavage of the AdoMetDC protein after synthesis [19]. Mammalian AdoMetDC is produced as a pro-enzyme which is rapidly cleaved into two differently sized subuni ...
... very few enzymes that uses pyruvate, instead of pyridoxal 5′-phosphate, as a cofactor [18]. The pyruvate is generated by an autocatalytical cleavage of the AdoMetDC protein after synthesis [19]. Mammalian AdoMetDC is produced as a pro-enzyme which is rapidly cleaved into two differently sized subuni ...
review on enhancement of glucose uptake and up
... adipocytes, and skeletal muscle cells. Medicinal plants or their active constituents that can up-regulate GLUT expression and translocation that helps in the treatment of insulin resistance and hyperglycemia. Evidence from insulin-resistant rodent models suggests that defective glucose transport in ...
... adipocytes, and skeletal muscle cells. Medicinal plants or their active constituents that can up-regulate GLUT expression and translocation that helps in the treatment of insulin resistance and hyperglycemia. Evidence from insulin-resistant rodent models suggests that defective glucose transport in ...
Regulation of E. coli Glycogen Phosphorylase Activity by HPr
... for PEP to pass the phosphoryl group directly to enzymes II. Third, the cellular concentration of HPr in E. coli is significantly higher than necessary for its role as a phosphocarrier, and there are between 10- and 20-fold more HPr molecules in a cell than enzyme I subunits (Mattoo and Waygood, 198 ...
... for PEP to pass the phosphoryl group directly to enzymes II. Third, the cellular concentration of HPr in E. coli is significantly higher than necessary for its role as a phosphocarrier, and there are between 10- and 20-fold more HPr molecules in a cell than enzyme I subunits (Mattoo and Waygood, 198 ...
Organelle Assembly in Yeast: Characterization of
... to the gene for the normally secreted enzyme invertase (Inv) contain sufficient sorting information to divert delivery of enzymatically active Inv to the yeast vacuole (2, 21). Mutants have been selected that missort and secrete such CPY-Inv hybrid proteins. The 'x,600 mutants isolated thus far have ...
... to the gene for the normally secreted enzyme invertase (Inv) contain sufficient sorting information to divert delivery of enzymatically active Inv to the yeast vacuole (2, 21). Mutants have been selected that missort and secrete such CPY-Inv hybrid proteins. The 'x,600 mutants isolated thus far have ...
Evolution of acidocalcisomes and their role in polyphosphate
... most eukaryotic microbes. ScVtc4 was identified as the catalytic subunit of the complex (Hothorn et al. 2009), thus explaining the conservation of this subunit in other fungi as well as in protists, which usually have only two of the four subunits present in yeast. Vtc4 also possesses an SPX domain. ...
... most eukaryotic microbes. ScVtc4 was identified as the catalytic subunit of the complex (Hothorn et al. 2009), thus explaining the conservation of this subunit in other fungi as well as in protists, which usually have only two of the four subunits present in yeast. Vtc4 also possesses an SPX domain. ...
Evolution of acidocalcisomes and their role in polyphosphate
... most eukaryotic microbes. ScVtc4 was identified as the catalytic subunit of the complex (Hothorn et al. 2009), thus explaining the conservation of this subunit in other fungi as well as in protists, which usually have only two of the four subunits present in yeast. Vtc4 also possesses an SPX domain. ...
... most eukaryotic microbes. ScVtc4 was identified as the catalytic subunit of the complex (Hothorn et al. 2009), thus explaining the conservation of this subunit in other fungi as well as in protists, which usually have only two of the four subunits present in yeast. Vtc4 also possesses an SPX domain. ...
Lens Major Intrinsic Protein (MIP)
... quenching of NBD emission was seen only when LUV reconstituted with junctional proteins were mixed with PS vesicles. The presence of the junctional proteins on PC-reconstituted vesicles was studied using SDS-PAGE. As in lens junction membranes, the most abundant protein reconstituted was MIP, being ...
... quenching of NBD emission was seen only when LUV reconstituted with junctional proteins were mixed with PS vesicles. The presence of the junctional proteins on PC-reconstituted vesicles was studied using SDS-PAGE. As in lens junction membranes, the most abundant protein reconstituted was MIP, being ...
Identification of Bioactive Peptide Sequences from Amaranth
... protein by antibodies; (2) evidence at a transcript level, which indicates that the existence of a protein has not been strictly proven but that expression data (such as existence of complementary deoxyribonucleic acids, real-time polymerase chain reaction, or Northern blots) indicate the existence ...
... protein by antibodies; (2) evidence at a transcript level, which indicates that the existence of a protein has not been strictly proven but that expression data (such as existence of complementary deoxyribonucleic acids, real-time polymerase chain reaction, or Northern blots) indicate the existence ...
The SR Protein SRp38 Represses Splicing in M Phase Cells
... regulation play key roles in cell growth control, differentiation, and disease (reviewed by Graveley, 2001; Grabowski and Black, 2001). Indeed, it has been estimated that expression of 35% or more of human genes involves alternative splicing (e.g., Mironov et al., 1999). Given the frequency, importa ...
... regulation play key roles in cell growth control, differentiation, and disease (reviewed by Graveley, 2001; Grabowski and Black, 2001). Indeed, it has been estimated that expression of 35% or more of human genes involves alternative splicing (e.g., Mironov et al., 1999). Given the frequency, importa ...
Lactobacillus plantarum - UEF Electronic Publications
... environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, makin ...
... environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, makin ...
The mitochondrial carnitine/acylcarnitine carrier: Function
... greatly helped the interpretation of experimental results obtained mainly by site-directed mutagenesis. In these studies one or more amino acids of CAC were mutated and the mutant CAC proteins were tested for activity after their incorporation into liposomes. The first amino acids of CAC which were s ...
... greatly helped the interpretation of experimental results obtained mainly by site-directed mutagenesis. In these studies one or more amino acids of CAC were mutated and the mutant CAC proteins were tested for activity after their incorporation into liposomes. The first amino acids of CAC which were s ...
Determinants in HIV-2 Env and tetherin required for
... positions 114 and 116, retained restrictive activity (data not shown), and these were further tested for sensitivity to both Ebola GP and ROD10 Env. As expected, both arttetherin and the two insertional mutants remained sensitive to the Ebola GP (Fig. 4b). However, the addition of the alanine motif ...
... positions 114 and 116, retained restrictive activity (data not shown), and these were further tested for sensitivity to both Ebola GP and ROD10 Env. As expected, both arttetherin and the two insertional mutants remained sensitive to the Ebola GP (Fig. 4b). However, the addition of the alanine motif ...
The relevance of carbon dioxide metabolism in
... foodstuff preservation against bacterial spoilage, a growthstimulating effect of CO2 has been reported for a limited number of lactic acid bacterial species. Detailed studies are available for Lactobacillus plantarum and Lactococcus lactis only (Wang et al., 1998, 2000; Nicoloff et al., 2000, 2005; ...
... foodstuff preservation against bacterial spoilage, a growthstimulating effect of CO2 has been reported for a limited number of lactic acid bacterial species. Detailed studies are available for Lactobacillus plantarum and Lactococcus lactis only (Wang et al., 1998, 2000; Nicoloff et al., 2000, 2005; ...
BTK, THE TYROSINE KINASE AFFECTED IN X-LINKED
... suggest a pivotal role for Btk in lymphohematopoietic growth and differentiation (16-21). The characteristics of the Tec family of PTKs are summarized in Table 1. Except for Bmx, which is expressed in bone marrow and endothelial cells, the members of the Tec family are mainly expressed in hematopoie ...
... suggest a pivotal role for Btk in lymphohematopoietic growth and differentiation (16-21). The characteristics of the Tec family of PTKs are summarized in Table 1. Except for Bmx, which is expressed in bone marrow and endothelial cells, the members of the Tec family are mainly expressed in hematopoie ...
1. The BCL-2 Family Reunion.
... large unstructured loop joining the inhibitory N terminus and the BH3-containing C terminus. Cleavage can be achieved by a variety of proteases; e.g., caspase-8 (via death receptors), granzyme B (cytotoxic lymphocytes), and caspase-2 (via heat shock). The proapoptotic function of BID is also enhance ...
... large unstructured loop joining the inhibitory N terminus and the BH3-containing C terminus. Cleavage can be achieved by a variety of proteases; e.g., caspase-8 (via death receptors), granzyme B (cytotoxic lymphocytes), and caspase-2 (via heat shock). The proapoptotic function of BID is also enhance ...
Expression of the LXR Protein in Human Atherosclerotic Lesions
... Human Monocyte-Derived Macrophages by the Monoclonal Antibody PPZ0412 Figure 2A indicates the results of immunoblotting using whole-cell extracts obtained from human monocytes and macrophages. The monoclonal antibody PPZ0412 bound to the 47-kDa protein in human monocyte-derived macrophages. The appa ...
... Human Monocyte-Derived Macrophages by the Monoclonal Antibody PPZ0412 Figure 2A indicates the results of immunoblotting using whole-cell extracts obtained from human monocytes and macrophages. The monoclonal antibody PPZ0412 bound to the 47-kDa protein in human monocyte-derived macrophages. The appa ...
Stimulation of glucose utilization in 3T3 adipocytes and rat
... lipolysis (rat adipocytes) up to 40-60%. The increased glucose utilization was correlated with a 3-4fold higher 2-deoxyglucose transport rate and amount of GLUT4 at the plasma membrane, as well as with increased activities of key metabolic enzymes (glycerol-3-phosphate acyltransferase, glycogen synt ...
... lipolysis (rat adipocytes) up to 40-60%. The increased glucose utilization was correlated with a 3-4fold higher 2-deoxyglucose transport rate and amount of GLUT4 at the plasma membrane, as well as with increased activities of key metabolic enzymes (glycerol-3-phosphate acyltransferase, glycogen synt ...
Molecular cloning, characterization and gene expression of an
... organisms from eubacteria to eukaryotes [9,20e26], they are essential, strongly expressed, and tightly regulated [27]. Catalase comprises four ferriphotophorphyrin groups per molecule, and its enzymatic activity in tissues varies greatly [28,29]. Each monomer harbors a single heme and nicotinamide a ...
... organisms from eubacteria to eukaryotes [9,20e26], they are essential, strongly expressed, and tightly regulated [27]. Catalase comprises four ferriphotophorphyrin groups per molecule, and its enzymatic activity in tissues varies greatly [28,29]. Each monomer harbors a single heme and nicotinamide a ...
Ideal Amino Acid Profile For Piglets
... various research programs to fill the gap of knowledge concerning the next limiting AA for piglets. A masterpiece of this program has been the work of Gloaguen (2012) about Valine, Isoleucine, Leucine, Histidine, Phenylalanine, Tyrosine and dietary protein reduction in piglets. These new outcomes, t ...
... various research programs to fill the gap of knowledge concerning the next limiting AA for piglets. A masterpiece of this program has been the work of Gloaguen (2012) about Valine, Isoleucine, Leucine, Histidine, Phenylalanine, Tyrosine and dietary protein reduction in piglets. These new outcomes, t ...
Protein stabilization: a common consequence of mutations
... dierence) and that this single mutation is both necessary and sucient for the phenotype. A major involvement of the proteasome in Myc degradation was con®rmed, but surprisingly, a dilysine motif adjacent to thr-61 proved not to be the ubiquitin target. Two other v-Myc genes which carry a mutation ...
... dierence) and that this single mutation is both necessary and sucient for the phenotype. A major involvement of the proteasome in Myc degradation was con®rmed, but surprisingly, a dilysine motif adjacent to thr-61 proved not to be the ubiquitin target. Two other v-Myc genes which carry a mutation ...