Assembly-Line Enzymology for Polyketide and Nonribosomal
... NRPS-mediated peptide elongations do not use an RNA template. Rather, the identity and ordering of protein domains (Figure 1d) in the synthetase constitutes the template (Figure 6). There are one or two gate-keeping domains in each NRPS module that specify the monomer to be selected and the chiralit ...
... NRPS-mediated peptide elongations do not use an RNA template. Rather, the identity and ordering of protein domains (Figure 1d) in the synthetase constitutes the template (Figure 6). There are one or two gate-keeping domains in each NRPS module that specify the monomer to be selected and the chiralit ...
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified
... FIG. 3. Purified reaction components for Sic1 ubiquitination assays. (A) Uba1 was purified from yeast strain RJD941 by NiNTA chromatography. This strain expresses hexahistidine‐ tagged Uba1 under control of a copper‐inducible promoter. A total of 2 g was loaded and run on an 8% SDS‐containing polya ...
... FIG. 3. Purified reaction components for Sic1 ubiquitination assays. (A) Uba1 was purified from yeast strain RJD941 by NiNTA chromatography. This strain expresses hexahistidine‐ tagged Uba1 under control of a copper‐inducible promoter. A total of 2 g was loaded and run on an 8% SDS‐containing polya ...
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... which is responsible for DnaA binding to acidic membranes. Domain IV (375–467) is essential for DNA binding and nucleoprotein complex formation. ...
... which is responsible for DnaA binding to acidic membranes. Domain IV (375–467) is essential for DNA binding and nucleoprotein complex formation. ...
Src protein–tyrosine kinase structure and regulation
... Src is expressed ubiquitously; however, brain, osteoclasts, and platelets express 5–200-fold higher levels of this protein than most other cells [2]. Alternatively spliced forms of Src, which contain 6- or 11-amino acid insertions in the SH3 domain (18- or 33-nucleotide insertions between exons 3 an ...
... Src is expressed ubiquitously; however, brain, osteoclasts, and platelets express 5–200-fold higher levels of this protein than most other cells [2]. Alternatively spliced forms of Src, which contain 6- or 11-amino acid insertions in the SH3 domain (18- or 33-nucleotide insertions between exons 3 an ...
original version
... which is responsible for DnaA binding to acidic membranes. Domain IV (375–467) is essential for DNA binding and nucleoprotein complex formation. ...
... which is responsible for DnaA binding to acidic membranes. Domain IV (375–467) is essential for DNA binding and nucleoprotein complex formation. ...
Isolation of Rat Transferrin Using CNBr-Activated
... Fig. Ib. Elution of pooled rat serum. For arrows see Figure la. on a CNBr-Sepharose column (3.5 X 1.6 cm.), to which Fractions 10-13 contain rat transferrin, J. Cliri. Chem. Clin. Biochem. / Vol. 14,1976 / No. 10 ...
... Fig. Ib. Elution of pooled rat serum. For arrows see Figure la. on a CNBr-Sepharose column (3.5 X 1.6 cm.), to which Fractions 10-13 contain rat transferrin, J. Cliri. Chem. Clin. Biochem. / Vol. 14,1976 / No. 10 ...
Effects of macromolecular crowding on protein folding and
... the presence of crowding agents, the yields of correctly folded protein are hardly affected. Identical results were obtained by refolding from 6 M guanidine HCl (GuHCl) as a denaturant (data not shown). Only at concentrations of crowding agents in excess of 250 g/l does the refolding yield decrease ...
... the presence of crowding agents, the yields of correctly folded protein are hardly affected. Identical results were obtained by refolding from 6 M guanidine HCl (GuHCl) as a denaturant (data not shown). Only at concentrations of crowding agents in excess of 250 g/l does the refolding yield decrease ...
Functional characterization of polypeptide release factor 1b in the
... linear amino acids, Pro-Ala-Thr (PAT), whereas RF2 recognizes UAA/UGA through Ser-Pro-Phe (SPF) [4,5]. In higher eukaryotes, there is only one RF-I, eRF1 (eukaryotic release factor 1), which can decode all three stop codons [6–8]. ...
... linear amino acids, Pro-Ala-Thr (PAT), whereas RF2 recognizes UAA/UGA through Ser-Pro-Phe (SPF) [4,5]. In higher eukaryotes, there is only one RF-I, eRF1 (eukaryotic release factor 1), which can decode all three stop codons [6–8]. ...
The aconitase of Escherichia cok purification of the
... Very little is known about the Escherichia coli aconitase and studies on the aconitase gene ( a m ) have been hindered by the lack of mutants deficient in aconitase. Genes encoding all of the citric acid cycle ...
... Very little is known about the Escherichia coli aconitase and studies on the aconitase gene ( a m ) have been hindered by the lack of mutants deficient in aconitase. Genes encoding all of the citric acid cycle ...
Phototropism: Growing towards an Understanding of
... NONPHOTOTROPIC HYPOCOTYL3 (NPH3) was originally identified as a phototropic loss-of-function mutant in Arabidopsis (Liscum and Briggs, 1995). NPH3 encodes a protein containing an N-terminal Broad-complex, Tramtrack, and Bric-á-brac (BTB) domain, a central NPH3 domain (Pfam, PF03000), and a C-terminal ...
... NONPHOTOTROPIC HYPOCOTYL3 (NPH3) was originally identified as a phototropic loss-of-function mutant in Arabidopsis (Liscum and Briggs, 1995). NPH3 encodes a protein containing an N-terminal Broad-complex, Tramtrack, and Bric-á-brac (BTB) domain, a central NPH3 domain (Pfam, PF03000), and a C-terminal ...
The Metabolism of Cellulose, Glucose and Starch by
... these produced from non-radioactive substrates. On addition of [14C]celluloseor rice starch, there was no increase or a decrease (up to 35 %) in the rate of production over 24 h. On incubation of starved protozoa (100000 organisms of isolate w grown in vitro) with cellulose or rice starch (4 mg) in ...
... these produced from non-radioactive substrates. On addition of [14C]celluloseor rice starch, there was no increase or a decrease (up to 35 %) in the rate of production over 24 h. On incubation of starved protozoa (100000 organisms of isolate w grown in vitro) with cellulose or rice starch (4 mg) in ...
Tetrahymena Contain Two Distinct and Unusual High Mobility Group
... Examination of the pattern of LG-1 and LG-2 synthesis during the sexual phase of the life cycle, conjugation, demonstrates that the synthesis of LG-1 and LG-2 is coordinately increased from basal levels during the differentiation of new macronuclei (7-13 h), suggesting that both of these proteins pl ...
... Examination of the pattern of LG-1 and LG-2 synthesis during the sexual phase of the life cycle, conjugation, demonstrates that the synthesis of LG-1 and LG-2 is coordinately increased from basal levels during the differentiation of new macronuclei (7-13 h), suggesting that both of these proteins pl ...
Domains of the Adenovirus E1A Protein Required for
... Figure 1. Structure of E1A mutants. The wild-type 243-aminoacid product of the 12S E1A RNA. The two regions that exhibit sequence conservation among various adenovirus serotypes (Kimelman et al. 1985) are indicated. Point mutations are indicated by arrows; the name of the mutant indicates the amino ...
... Figure 1. Structure of E1A mutants. The wild-type 243-aminoacid product of the 12S E1A RNA. The two regions that exhibit sequence conservation among various adenovirus serotypes (Kimelman et al. 1985) are indicated. Point mutations are indicated by arrows; the name of the mutant indicates the amino ...
High pKa variability of cysteine residues in structural databases and
... any other structure in the dataset) were kept; (iii) only protein with more than 100 and less than 1000 amino acids were considered. Among the PDB entries passing these filters (circa 13000, as of April 2013), 750 structures were randomly selected for further analysis, containing a total number of 4 ...
... any other structure in the dataset) were kept; (iii) only protein with more than 100 and less than 1000 amino acids were considered. Among the PDB entries passing these filters (circa 13000, as of April 2013), 750 structures were randomly selected for further analysis, containing a total number of 4 ...
Formation of Helical Hairpins during Membrane Protein Integration
... of the P2 domain, an N-glycosylation site (Asn-SerThr) was introduced 20 amino acid residues downstream of H2; in constructs where the poly-Leu stretch spans the membrane only once, this site will be glycosylated by the lumenally disposed oligosaccharyl transferase enzyme (Figure 1(a), left), while ...
... of the P2 domain, an N-glycosylation site (Asn-SerThr) was introduced 20 amino acid residues downstream of H2; in constructs where the poly-Leu stretch spans the membrane only once, this site will be glycosylated by the lumenally disposed oligosaccharyl transferase enzyme (Figure 1(a), left), while ...
FtsZ - Cytoskeleton, Inc.
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
Expression and Characterization of Cynomolgus Monkey
... with human intestinal microsomes. Additionally, a much higher intestinal extraction was observed in monkeys, as compared with humans, for midazolam (MDZ), nifedipine, amitriptyline, propranolol, and timolol, drugs metabolized by human CYP3A4 (h3A4), 2C19, 2D6, and 1A2, respectively, suggesting that ...
... with human intestinal microsomes. Additionally, a much higher intestinal extraction was observed in monkeys, as compared with humans, for midazolam (MDZ), nifedipine, amitriptyline, propranolol, and timolol, drugs metabolized by human CYP3A4 (h3A4), 2C19, 2D6, and 1A2, respectively, suggesting that ...
Protein relative abundance patterns associated with sucrose
... within a smaller subset of subjects. One of the key findings was that healthy sites from different people were broadly similar with respect to their functional profiles, even though there was extensive individual variation in their taxonomic profiles [20]. It is possible that the “conservation of fu ...
... within a smaller subset of subjects. One of the key findings was that healthy sites from different people were broadly similar with respect to their functional profiles, even though there was extensive individual variation in their taxonomic profiles [20]. It is possible that the “conservation of fu ...
Free Full Text ( Final Version , 5mb ) - EUR RePub
... Rivett, 1989a). One is a 1500 kDa (26S) particle, capable of degrading ubiquitinated proteins in vitro in an ATP-dependent fashion (Fagan eta!, 1987; Hough et a!, 1987). The other is smaller (-600 kDa) and has been given many names, the most common being multicatalytic proteinase complex, MCPC (revi ...
... Rivett, 1989a). One is a 1500 kDa (26S) particle, capable of degrading ubiquitinated proteins in vitro in an ATP-dependent fashion (Fagan eta!, 1987; Hough et a!, 1987). The other is smaller (-600 kDa) and has been given many names, the most common being multicatalytic proteinase complex, MCPC (revi ...
Saccharomyces species in the Production of Beer
... potable), wort is by far the most intricate. Therefore, when yeast is pitched (inoculated) into wort it potable), wort is by far the most intricate. Therefore, when yeast is pitched (inoculated) into wort it is is introduced into a complex environment because it consists of simple sugars, dextrins, ...
... potable), wort is by far the most intricate. Therefore, when yeast is pitched (inoculated) into wort it potable), wort is by far the most intricate. Therefore, when yeast is pitched (inoculated) into wort it is is introduced into a complex environment because it consists of simple sugars, dextrins, ...
Protein and Amino Acid Metabolism During and After Exercise
... and they are chiefly found in muscle, accounting for ∼65% of the total muscle protein. Thus their primary amino acid composition has a substantial effect on the composition of the amino acids released from protein in muscle during protein breakdown and withdrawn from the free pool during protein syn ...
... and they are chiefly found in muscle, accounting for ∼65% of the total muscle protein. Thus their primary amino acid composition has a substantial effect on the composition of the amino acids released from protein in muscle during protein breakdown and withdrawn from the free pool during protein syn ...
Regulation of KNOLLE syntaxin - Journal of Cell Science
... benzyl aminopurine (Sigma) pH 5.7 with KOH, and 0.005% SILWET L77 (Osi Specialities). T1 seeds were bulk-harvested (each seed representing a single transformation event; Bechtold et al., 2000; Desfeux et al., 2000; Ye et al., 1999), sown on soil and selected for transformants by spraying BASTA® (183 ...
... benzyl aminopurine (Sigma) pH 5.7 with KOH, and 0.005% SILWET L77 (Osi Specialities). T1 seeds were bulk-harvested (each seed representing a single transformation event; Bechtold et al., 2000; Desfeux et al., 2000; Ye et al., 1999), sown on soil and selected for transformants by spraying BASTA® (183 ...
Antidepressant specificity of serotonin transporter suggested by three LeuT–SSRI structures Zheng Zhou
... SSRIs bind directly to the serotonin-transporter protein and inhibit neurotransmitter recycling, making them effective drugs for the treatment of depressive disorders1,2. SSRIs, however, are rather promiscuous in that they also bind to the homologous norepinephrine and dopamine transporters (NET and ...
... SSRIs bind directly to the serotonin-transporter protein and inhibit neurotransmitter recycling, making them effective drugs for the treatment of depressive disorders1,2. SSRIs, however, are rather promiscuous in that they also bind to the homologous norepinephrine and dopamine transporters (NET and ...