Tyrosine phosphorylation at the MET catalytic doamin and
... RON proteins (extracellular domains, R&D Systems) were incubated at 10-fold higher (4 to 400 pM) concentration than rMET (0.4-40 pM) in the full-length MET immunoassay. In immunoassay format, the cross-reactivity of the AF276 antibody with the receptor tyrosine kinase RON was < 5%, and no detectable ...
... RON proteins (extracellular domains, R&D Systems) were incubated at 10-fold higher (4 to 400 pM) concentration than rMET (0.4-40 pM) in the full-length MET immunoassay. In immunoassay format, the cross-reactivity of the AF276 antibody with the receptor tyrosine kinase RON was < 5%, and no detectable ...
The AP-3 adaptor complex is required for vacuolar function
... brassinosteroid receptor [23]) PM proteins showed aggregation in intracellular compartments (Figure 1C-1H). The pronounced intracellular accumulation of diverse proteins had surprisingly little impact on the plant morphology because pat4-1 did not show any abnormal phenotypes under standard growth c ...
... brassinosteroid receptor [23]) PM proteins showed aggregation in intracellular compartments (Figure 1C-1H). The pronounced intracellular accumulation of diverse proteins had surprisingly little impact on the plant morphology because pat4-1 did not show any abnormal phenotypes under standard growth c ...
Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell
... force microscope to detect surface accessibility of “molecular rulers” consisting of versions of the plasma membrane sensor Wsc1 with different lengths (Dupres et al. 2010; Yamaguchi et al. 2011). The wall’s major components are b1,3- and b1,6-linked glucans, mannoproteins, and chitin, which can be ...
... force microscope to detect surface accessibility of “molecular rulers” consisting of versions of the plasma membrane sensor Wsc1 with different lengths (Dupres et al. 2010; Yamaguchi et al. 2011). The wall’s major components are b1,3- and b1,6-linked glucans, mannoproteins, and chitin, which can be ...
Cloning vectors for the expression of green fluorescent protein
... A series of versatile cloning vectors has been constructed that facilitate the expression of protein fusions to the Aequorea victoria green fluorescent protein (GFP) in plant cells. Amino-terminal- and carboxy-terminal protein fusions have been created and visualized by epifluorescence microscopy, b ...
... A series of versatile cloning vectors has been constructed that facilitate the expression of protein fusions to the Aequorea victoria green fluorescent protein (GFP) in plant cells. Amino-terminal- and carboxy-terminal protein fusions have been created and visualized by epifluorescence microscopy, b ...
Site-selective incorporation and ligation of
... chains is present within a protein. In recent years more nuanced protein labelling strategies have emerged; these strategies primarily focus around incorporating a unique functional ‘handle’ into a protein that is not naturally present within the proteinogenic amino acid side chains. The tagged prot ...
... chains is present within a protein. In recent years more nuanced protein labelling strategies have emerged; these strategies primarily focus around incorporating a unique functional ‘handle’ into a protein that is not naturally present within the proteinogenic amino acid side chains. The tagged prot ...
Cellular functions of the BRCA tumour
... mice exhibit indistinguishable phenotypes indicative of a shared functional role in vivo [13,38,39]. Three-dimensional structural analysis of the BRCA1–BARD1 RING heterodimer complex indicates that these proteins interact through an extensive four-helix bundle formed by helices that flank the core R ...
... mice exhibit indistinguishable phenotypes indicative of a shared functional role in vivo [13,38,39]. Three-dimensional structural analysis of the BRCA1–BARD1 RING heterodimer complex indicates that these proteins interact through an extensive four-helix bundle formed by helices that flank the core R ...
A Comprehensive Mutational Analysis of the
... The Arabidopsis thaliana RESISTANCE TO POWDERY MILDEW8.2 (RPW8.2) protein is specifically targeted to the extrahaustorial membrane (EHM) encasing the haustorium, or fungal feeding structure, where RPW8.2 activates broad-spectrum resistance against powdery mildew pathogens. How RPW8.2 activates defens ...
... The Arabidopsis thaliana RESISTANCE TO POWDERY MILDEW8.2 (RPW8.2) protein is specifically targeted to the extrahaustorial membrane (EHM) encasing the haustorium, or fungal feeding structure, where RPW8.2 activates broad-spectrum resistance against powdery mildew pathogens. How RPW8.2 activates defens ...
FKBP12-rapamycin-associated protein associates
... FKBP12-rapamycin associated protein (FRAP, also known as mTOR or RAFT) is the founding member of the phosphatidylinositol kinase-related kinase family and functions as a sensor of physiological signals that regulate cell growth. Signals integrated by FRAP include nutrients, cAMP levels, and osmotic ...
... FKBP12-rapamycin associated protein (FRAP, also known as mTOR or RAFT) is the founding member of the phosphatidylinositol kinase-related kinase family and functions as a sensor of physiological signals that regulate cell growth. Signals integrated by FRAP include nutrients, cAMP levels, and osmotic ...
Accumulation of xylem transported protein at pit membranes and
... pathway of least resistance during sap transport from one vessel to another. Nevertheless, they may account for around 50% of overall xylem resistance to flow (see review by Choat et al., 2008). Proteomic investigations using gel separations and, more recently, tandem mass spectrometry have identifi ...
... pathway of least resistance during sap transport from one vessel to another. Nevertheless, they may account for around 50% of overall xylem resistance to flow (see review by Choat et al., 2008). Proteomic investigations using gel separations and, more recently, tandem mass spectrometry have identifi ...
Prediction of protease substrates using sequence
... substrates to induce apoptosis in virally infected and neoplastic cells. While substrates for both types of proteases have been determined experimentally, there are many more yet to be discovered in humans and other metazoans. Here, we present a bioinformatics method based on support vector machine ...
... substrates to induce apoptosis in virally infected and neoplastic cells. While substrates for both types of proteases have been determined experimentally, there are many more yet to be discovered in humans and other metazoans. Here, we present a bioinformatics method based on support vector machine ...
Enzymatic activation of sulfur for incorporation into biomolecules in
... given that, in addition to the FeS clusters, the thionucleosides 4-thiouridine and 5-methylaminomethyl-2-thiouridine (Lauhon & Kambampati, 2000; Lauhon, 2002) as well as thiamin (Lauhon & Kambampati, 2000) also receive their sulfur from IscS. Indeed, other sulfur-containing molecules may be biosynth ...
... given that, in addition to the FeS clusters, the thionucleosides 4-thiouridine and 5-methylaminomethyl-2-thiouridine (Lauhon & Kambampati, 2000; Lauhon, 2002) as well as thiamin (Lauhon & Kambampati, 2000) also receive their sulfur from IscS. Indeed, other sulfur-containing molecules may be biosynth ...
The Family of Berberine Bridge Enzyme-like
... and Dr. Domen Zafred for joining the team BBE. It was a pleasure to unravel the mysteries of BBE-like protein family with you in joined forces. Furthermore I would like to thank my thesis committee members Prof. Karl Gruber and Prof. Wolfgang Kroutil. Karl kindly gave me an insight into structural b ...
... and Dr. Domen Zafred for joining the team BBE. It was a pleasure to unravel the mysteries of BBE-like protein family with you in joined forces. Furthermore I would like to thank my thesis committee members Prof. Karl Gruber and Prof. Wolfgang Kroutil. Karl kindly gave me an insight into structural b ...
Transendothelial Transport and Its Role in Therapeutics
... and into the brain have been widely explained. In addition, roles of various receptors, ATP powered pumps, channels, and transporters in transport of vital molecules in maintenance of homeostasis and normal body functions have been described in detail. Major role of integral membrane proteins, carri ...
... and into the brain have been widely explained. In addition, roles of various receptors, ATP powered pumps, channels, and transporters in transport of vital molecules in maintenance of homeostasis and normal body functions have been described in detail. Major role of integral membrane proteins, carri ...
as a PDF
... volume of solution E (formic acid/acetic acid/H 2 0, 50 :156 :1,794, pH 1 .9) and subjected to high-voltage electrophoresis in the first dimension on TLC cellulose plates (Merck) in the same buffer at 1 kV at 20°C for 40 min . Ascending chromatography in the second dimension was carried out in solut ...
... volume of solution E (formic acid/acetic acid/H 2 0, 50 :156 :1,794, pH 1 .9) and subjected to high-voltage electrophoresis in the first dimension on TLC cellulose plates (Merck) in the same buffer at 1 kV at 20°C for 40 min . Ascending chromatography in the second dimension was carried out in solut ...
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... component (20%) in the eye lens of the gecko Lepidodactylus lugubris. Limited peptide sequence analysis earlier revealed that it belongs to the aldo-keto reductase superfamily, as does the frog lens -crystallin. We have now determined the complete cDNA sequence of Bcrystallin and established that ...
... component (20%) in the eye lens of the gecko Lepidodactylus lugubris. Limited peptide sequence analysis earlier revealed that it belongs to the aldo-keto reductase superfamily, as does the frog lens -crystallin. We have now determined the complete cDNA sequence of Bcrystallin and established that ...
E. coli
... resulting mutants (Fig.7, 8). All three disulfide bonds formed at fixed positions are required for full expression of the biological activity of STb. It has some fexibilities in its conformation to exert toxic activity and that the role of each disulfide bond in exerting toxic activity is not qu ...
... resulting mutants (Fig.7, 8). All three disulfide bonds formed at fixed positions are required for full expression of the biological activity of STb. It has some fexibilities in its conformation to exert toxic activity and that the role of each disulfide bond in exerting toxic activity is not qu ...
PfPI3K, a phosphatidylinositol-3 kinase from
... protein. PfPI3K shares high homology with the catalytic and signature helical domain of PI3Ks (supplemental Table 1). PI3Ks are classified based on their domain architecture, mechanism of regulation, and substrate affinities.3 Comparison of vacuolar protein-sorting protein 34 (vps34), a class III PI ...
... protein. PfPI3K shares high homology with the catalytic and signature helical domain of PI3Ks (supplemental Table 1). PI3Ks are classified based on their domain architecture, mechanism of regulation, and substrate affinities.3 Comparison of vacuolar protein-sorting protein 34 (vps34), a class III PI ...
Facilitated transport of small molecules and ions for energy
... because the water-mediated vehicle mechanism may also play a pivotal role in facilitated transport. In a broader sense, when small molecules with active carriers are doped into polymer membranes, the carriers might migrate if the polymer matrix is plasticized or swollen by a solvent. This type of me ...
... because the water-mediated vehicle mechanism may also play a pivotal role in facilitated transport. In a broader sense, when small molecules with active carriers are doped into polymer membranes, the carriers might migrate if the polymer matrix is plasticized or swollen by a solvent. This type of me ...
Formation of Monoterpenes in Antirrhinum majus
... The precursor of all monoterpenes is the C10 acyclic intermediate geranyl diphosphate (GPP), which is formed from the C5 compounds isopentenyl diphosphate and dimethylallyl diphosphate by GPP synthase (GPPS). We have discovered that Antirrhinum majus (snapdragon) and Clarkia breweri, two species who ...
... The precursor of all monoterpenes is the C10 acyclic intermediate geranyl diphosphate (GPP), which is formed from the C5 compounds isopentenyl diphosphate and dimethylallyl diphosphate by GPP synthase (GPPS). We have discovered that Antirrhinum majus (snapdragon) and Clarkia breweri, two species who ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
Specificity of the Organic Acid Activation of
... only L-malate and pyruvate caused a significant increase in NADH-dependent oxygen uptake (Fig. 2B). These results were also observed with ubiquinol-1 as substrate, but with this substrate D- and L-malate stimulated only after addition of 0.1 mM NAD+ (not shown). ME Oxidizes Both ...
... only L-malate and pyruvate caused a significant increase in NADH-dependent oxygen uptake (Fig. 2B). These results were also observed with ubiquinol-1 as substrate, but with this substrate D- and L-malate stimulated only after addition of 0.1 mM NAD+ (not shown). ME Oxidizes Both ...
Adjeitey_Cyril _Nii-Klu_2013_ thesis
... The etiology of obesity is complex and obesity currently ranks highly amongst diseases that are known to cause major health problems. Currently, 34% of adults in the United States are considered obese which has made obesity a major contributor to disease burden in the United States (22, 107, 108). I ...
... The etiology of obesity is complex and obesity currently ranks highly amongst diseases that are known to cause major health problems. Currently, 34% of adults in the United States are considered obese which has made obesity a major contributor to disease burden in the United States (22, 107, 108). I ...
Endoplasmic reticulum–mitochondria contacts: function of the junction
... microtubules by factors that associate with the microtubule motor kinesin 1. In the case of mitochondria, this occurs through kinesin 1 binding to the cytoplasmic protein MILTON, which in turn binds MIRO on the outer mitochondrial membrane. MIRO is important for mitochondrial movement. Less is known ...
... microtubules by factors that associate with the microtubule motor kinesin 1. In the case of mitochondria, this occurs through kinesin 1 binding to the cytoplasmic protein MILTON, which in turn binds MIRO on the outer mitochondrial membrane. MIRO is important for mitochondrial movement. Less is known ...