Isolation and Characterization of a Novel Ribosome
... Vecchio Blanco et al., 1997). Importantly, the data indicated that highly conserved hydrophobic residues reported in the N-terminal region of all other RIPs, such as a Tyr-14 and Phe-17 (Funatsu et al., 1991) were found in the N-terminal region of the hairy root RIP. Based on these results, we concl ...
... Vecchio Blanco et al., 1997). Importantly, the data indicated that highly conserved hydrophobic residues reported in the N-terminal region of all other RIPs, such as a Tyr-14 and Phe-17 (Funatsu et al., 1991) were found in the N-terminal region of the hairy root RIP. Based on these results, we concl ...
Shigella boydii Involved in O-antigen Synthesis By
... 3.3.1 Donor substrate specificity .........................................................................- 58 3.3.2 Role of pyrophosphate in acceptor substrate specificity ..............................- 60 3.3.3 Screening of compounds as potential inhibitors ..................................... ...
... 3.3.1 Donor substrate specificity .........................................................................- 58 3.3.2 Role of pyrophosphate in acceptor substrate specificity ..............................- 60 3.3.3 Screening of compounds as potential inhibitors ..................................... ...
Dopamine transporter: involvement in selective dopaminergic
... efflux turnover through the transporter may be an important factor for MPPþ toxicity (Kitayama et al., 1998). Some mutations of the DAT protein resulting in enhanced reverse transport compared to that of wild type DAT (Y273A; double mutant S353A=S335A; Y533F) leads to decreased sensitivity of the ce ...
... efflux turnover through the transporter may be an important factor for MPPþ toxicity (Kitayama et al., 1998). Some mutations of the DAT protein resulting in enhanced reverse transport compared to that of wild type DAT (Y273A; double mutant S353A=S335A; Y533F) leads to decreased sensitivity of the ce ...
PCNA Protein Expression during Spermatogenesis of the
... entire open reading frame of the PCNA yielded a single band (data not shown), indicating that only one species of mRNA was responsible for the production of the two PCNA proteins. To further characterize these two PCNA proteins, we used antibodies that recognized different epitopes of eel PCNA (amin ...
... entire open reading frame of the PCNA yielded a single band (data not shown), indicating that only one species of mRNA was responsible for the production of the two PCNA proteins. To further characterize these two PCNA proteins, we used antibodies that recognized different epitopes of eel PCNA (amin ...
Journal of Microbiology and Biotechnology
... crystal structure of the IPDC from E. cloacae has recently been determined, and the native enzyme was estimated to be around 240 kDa, suggesting a homotetramer structure [27]. The enzyme is dependent on Mg2+ and thiamine diphosphate (ThDP) as cofactors, and has higher affinity for the substrate IPA ...
... crystal structure of the IPDC from E. cloacae has recently been determined, and the native enzyme was estimated to be around 240 kDa, suggesting a homotetramer structure [27]. The enzyme is dependent on Mg2+ and thiamine diphosphate (ThDP) as cofactors, and has higher affinity for the substrate IPA ...
The Role in Translation of Editing and Multi
... evolutionarily conserved. Tyrosyl-tRNA synthetases are among the aaRSs lacking any known editing activity. The high specificity displayed by this aaRSs is achieved by taking advantage of the unique structural and chemical properties of certain amino acids, leading to favorable binding affinities of ...
... evolutionarily conserved. Tyrosyl-tRNA synthetases are among the aaRSs lacking any known editing activity. The high specificity displayed by this aaRSs is achieved by taking advantage of the unique structural and chemical properties of certain amino acids, leading to favorable binding affinities of ...
Structural and functional validation of S
... have not been successful in clinical assessment, creating the need for further research in identifying novel inhibitors. ...
... have not been successful in clinical assessment, creating the need for further research in identifying novel inhibitors. ...
9. proteins i
... (enzymes) are largely responsible for determining the phenotype or properties of a cell in a particular environment. The total hereditary material of the cell or genotype dictates which type of protein the cell can produce. In fact, the proteins have built into their structure the information that i ...
... (enzymes) are largely responsible for determining the phenotype or properties of a cell in a particular environment. The total hereditary material of the cell or genotype dictates which type of protein the cell can produce. In fact, the proteins have built into their structure the information that i ...
Molecular Machinery Regulating Exocytosis
... there are also Golgi-specific Rabs (Rab30, Rab33, Rab34, Rab43) and Rabs relating to the traffic to (Rab43) and from (Rab10) the Golgi. Rab proteins usually play positive roles in anterograde membrane trafficking, but the exact nature of their involvement (in vesicle budding, biogenesis, transport, ...
... there are also Golgi-specific Rabs (Rab30, Rab33, Rab34, Rab43) and Rabs relating to the traffic to (Rab43) and from (Rab10) the Golgi. Rab proteins usually play positive roles in anterograde membrane trafficking, but the exact nature of their involvement (in vesicle budding, biogenesis, transport, ...
Full-Text PDF
... Sandaracinobacter sibiricus (RB 16-17), and Roseococcus thiosulfatophilus (RB3) [26–30]. They were grown aerobically in the dark at their optimal temperature (28 °C) on an incubator shaker (200 rpm) in liquid rich organic (RO) or liquid minimal salts (MS) media [30,31] containing either glutamate, p ...
... Sandaracinobacter sibiricus (RB 16-17), and Roseococcus thiosulfatophilus (RB3) [26–30]. They were grown aerobically in the dark at their optimal temperature (28 °C) on an incubator shaker (200 rpm) in liquid rich organic (RO) or liquid minimal salts (MS) media [30,31] containing either glutamate, p ...
Essays41 Chap03 - Essays in Biochemistry
... easily cover this distance by diffusion, and by use of these substrates it has been found that archaebacterial proteasomes preferentially hydrolyse peptide bonds at the C-terminal side of large hydrophobic amino-acid residues, thus exhibiting chymotrypsin-like specificity. However, within protein su ...
... easily cover this distance by diffusion, and by use of these substrates it has been found that archaebacterial proteasomes preferentially hydrolyse peptide bonds at the C-terminal side of large hydrophobic amino-acid residues, thus exhibiting chymotrypsin-like specificity. However, within protein su ...
Molecular Affinity and Permeability of Different Molecular Weight
... period, the adsorptive capacity curve stayed flat after 1 h. Some polymers that have carboxyl groups in the molecule, such as poly(itaconic acid) chitosan, have strong affinity (30). Therefore, the reason for the low permeability of the bovine serum protein on the chitosan membrane can be explained ...
... period, the adsorptive capacity curve stayed flat after 1 h. Some polymers that have carboxyl groups in the molecule, such as poly(itaconic acid) chitosan, have strong affinity (30). Therefore, the reason for the low permeability of the bovine serum protein on the chitosan membrane can be explained ...
Ubiquitination and Auxin Signaling: A Degrading Story
... and subsequently was shown to interact with either of the Arabidopsis Skp1-like proteins, ASK1 or ASK2, and the cullin AtCUL1 to form the functional SCF TIR1 (Gray et al., 1999, 2001). The idea that regulated protein degradation via SCFTIR1 is required for proper auxin signaling was strengthened by ...
... and subsequently was shown to interact with either of the Arabidopsis Skp1-like proteins, ASK1 or ASK2, and the cullin AtCUL1 to form the functional SCF TIR1 (Gray et al., 1999, 2001). The idea that regulated protein degradation via SCFTIR1 is required for proper auxin signaling was strengthened by ...
Anal. Chem. (2011) - American Chemical Society
... approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative cleavage of peptides at the C-terminus of tyrosine residues was performed at potentials between 0.5 and 1.5 V (vs a quasi palladium (Pd) reference electrode).27 A recent detailed stu ...
... approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative cleavage of peptides at the C-terminus of tyrosine residues was performed at potentials between 0.5 and 1.5 V (vs a quasi palladium (Pd) reference electrode).27 A recent detailed stu ...
BIOGENESIS OF MITOCHONDRIAL ATPase Studies on
... binding of F 1 to mitochondrial membrane and thus is one of the components which are essential for cold-stable and oligomycin-sensitive ATPase. The activity of OSCP increases during glucose derepression two to three fold as shown by reconstitution experiments with OSCP-enriched fractions [11]. The e ...
... binding of F 1 to mitochondrial membrane and thus is one of the components which are essential for cold-stable and oligomycin-sensitive ATPase. The activity of OSCP increases during glucose derepression two to three fold as shown by reconstitution experiments with OSCP-enriched fractions [11]. The e ...
- Wiley Online Library
... Secretion and/or translocation of some TTSS effectors in several animal pathogens is dependent on the function of customized chaperones. These type III chaperones appear to prevent the premature aggregation of effectors in the cytoplasm, to maintain effectors in a state competent for type III secret ...
... Secretion and/or translocation of some TTSS effectors in several animal pathogens is dependent on the function of customized chaperones. These type III chaperones appear to prevent the premature aggregation of effectors in the cytoplasm, to maintain effectors in a state competent for type III secret ...
Johansson L, Gafvelin G, Arnér ES. Selenocysteine in proteins
... for the synthesis of this single protein, clearly suggesting a major importance of maintaining thioredoxin reductase as a selenoprotein for that organism. Interestingly, however, insects have developed a highly reactive thioredoxin reductase, which is also essential [35] but which is not a selenopro ...
... for the synthesis of this single protein, clearly suggesting a major importance of maintaining thioredoxin reductase as a selenoprotein for that organism. Interestingly, however, insects have developed a highly reactive thioredoxin reductase, which is also essential [35] but which is not a selenopro ...
2006 Program
... “The S. aureus Hss/Hrt systems sense an abundant host marker of invasive infection and modulate virulence” Jared D. Heffron and David L. Popham “Germination specific lytic enzyme homologs in Bacillus anthracis spores” Benjamin C. Orsburn and David L. Popham “Factors contributing to the heat resistan ...
... “The S. aureus Hss/Hrt systems sense an abundant host marker of invasive infection and modulate virulence” Jared D. Heffron and David L. Popham “Germination specific lytic enzyme homologs in Bacillus anthracis spores” Benjamin C. Orsburn and David L. Popham “Factors contributing to the heat resistan ...
Supporting document 1 Safety assessment
... properties of AHAS expressed in CV127. These studies demonstrated that the AHAS protein expressed in CV127 is as expected in terms of its physicochemical and functional properties but the mature form of protein is slightly larger (by 34 amino acids) than anticipated due to the N-terminal signal pept ...
... properties of AHAS expressed in CV127. These studies demonstrated that the AHAS protein expressed in CV127 is as expected in terms of its physicochemical and functional properties but the mature form of protein is slightly larger (by 34 amino acids) than anticipated due to the N-terminal signal pept ...
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody
... relatively weak IgG and IgA responses in human sera, although it had strong antibody responses to other EBV proteins. Higher IgG reactivity was found in NPC patients (P ⴝ 0.015) than in regional Indonesian controls or EBV-negative individuals (P < 0.001). IgA responses to native BARF1 were marginal. ...
... relatively weak IgG and IgA responses in human sera, although it had strong antibody responses to other EBV proteins. Higher IgG reactivity was found in NPC patients (P ⴝ 0.015) than in regional Indonesian controls or EBV-negative individuals (P < 0.001). IgA responses to native BARF1 were marginal. ...
Mitochondrial Dynamics and the ER: The Plant Perspective
... A model for the mixing and unmixing of mitochondrial content was proposed, describing a separation of dysfunctional mitochondria from the pool of fusing mitochondria, and their targeting to autophagosomes (Twig et al., 2008b). In mammalian cells mitochondrial fusion triggers fission which in turn is ...
... A model for the mixing and unmixing of mitochondrial content was proposed, describing a separation of dysfunctional mitochondria from the pool of fusing mitochondria, and their targeting to autophagosomes (Twig et al., 2008b). In mammalian cells mitochondrial fusion triggers fission which in turn is ...
mitochondrial biogenesis during
... mitochondria increases with increasing times of incubation of the cyst at 30 ° C . The proteinsynthesizing activity was not inhibited by cycloheximide (100 µg/ml), while chloramphenicol (200 µg/ml) caused over 98% inhibition . These results indicate that mitochondrial protein synthesis was measured ...
... mitochondria increases with increasing times of incubation of the cyst at 30 ° C . The proteinsynthesizing activity was not inhibited by cycloheximide (100 µg/ml), while chloramphenicol (200 µg/ml) caused over 98% inhibition . These results indicate that mitochondrial protein synthesis was measured ...
- Wiley Online Library
... accompanied with the reduction of AnPRS activity causes the formation of hyper-septation as well as the restoration of septation and conidiation in the absence of SEPH. Clearly, the timing and positioning of septation is related to AnPRS activity. Moreover, with the extensive yeast two-hybrid analys ...
... accompanied with the reduction of AnPRS activity causes the formation of hyper-septation as well as the restoration of septation and conidiation in the absence of SEPH. Clearly, the timing and positioning of septation is related to AnPRS activity. Moreover, with the extensive yeast two-hybrid analys ...