The role of c-di-GMP signaling in an Aeromonas veronii biovar
... PhP groups of Aeromonas. Within 18 PhP clonal groups, biofilm formation scores are shown for six groups. ...
... PhP groups of Aeromonas. Within 18 PhP clonal groups, biofilm formation scores are shown for six groups. ...
Arabidopsis RTM1 and RTM2 Genes Function in Phloem to Restrict
... Extracts from roots, rosette leaves, stems, and inflorescence tissue of the TEV-restrictive ecotype Col-0 (RTM1/RTM1) were tested for RTM1 by immunoblot analysis (Fig. 1A). Although anti-RTM1 recognized purified RTM1-His6, no specific bands were detected in any Col-0 tissue type. Tissue from TEV-res ...
... Extracts from roots, rosette leaves, stems, and inflorescence tissue of the TEV-restrictive ecotype Col-0 (RTM1/RTM1) were tested for RTM1 by immunoblot analysis (Fig. 1A). Although anti-RTM1 recognized purified RTM1-His6, no specific bands were detected in any Col-0 tissue type. Tissue from TEV-res ...
The Case of Protein Kinase CK2
... between the nucleus and the cytoplasm (Li et al., 1997). This part of the protein also contains two motifs that have been previously characterized as motifs that regulate cyclin degradation. The CK2β regulatory subunit has a sequence resembling the nine-amino-acid motif called the destruction box, w ...
... between the nucleus and the cytoplasm (Li et al., 1997). This part of the protein also contains two motifs that have been previously characterized as motifs that regulate cyclin degradation. The CK2β regulatory subunit has a sequence resembling the nine-amino-acid motif called the destruction box, w ...
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... Analysis of acidic proteins by two-dimensional gel electrophoresis The patterns of polypeptide synthesis of oocytes labelled from either 0 to 3 h or from 18 to 21 h (i.e. after culture) are shown in Figs 3 and 4 respectively. These profiles confirm that maturation is accompanied by major changes in ...
... Analysis of acidic proteins by two-dimensional gel electrophoresis The patterns of polypeptide synthesis of oocytes labelled from either 0 to 3 h or from 18 to 21 h (i.e. after culture) are shown in Figs 3 and 4 respectively. These profiles confirm that maturation is accompanied by major changes in ...
Lewis lung carcinoma regulation of mechanical stretch - AJP-Cell
... solution (Advanced Biomatrix, San Diego, CA; diluted into 1 mg/ml by serum-free DMEM) was applied onto one edge of Silastic membrane on each stretching device. The collagen solution was then drawn across the substrate with a sterile cell scraper. Excess collagen was aspirated, and the culture dish c ...
... solution (Advanced Biomatrix, San Diego, CA; diluted into 1 mg/ml by serum-free DMEM) was applied onto one edge of Silastic membrane on each stretching device. The collagen solution was then drawn across the substrate with a sterile cell scraper. Excess collagen was aspirated, and the culture dish c ...
Hydrogels for protein delivery in tissue engineering ⁎ Roberta Censi
... loading process. Loading is done by incubation of the preformed gel with the protein of interest or by adding the protein to the hydrogel forming monomers/prepolymers. In recent years, attention is shifted to in situ gelling systems which preferably are liquid before administration and gellify at th ...
... loading process. Loading is done by incubation of the preformed gel with the protein of interest or by adding the protein to the hydrogel forming monomers/prepolymers. In recent years, attention is shifted to in situ gelling systems which preferably are liquid before administration and gellify at th ...
Profilin regulates the activity of p42 , a novel Myb
... Profilins, regulators of cytoplasmic actin dynamics, also bind to several nuclear proteins but the significance of these interactions is mostly unclear. Here, we describe a novel Myb-related transcription factor, p42POP, as a new ligand for profilin and show that profilin regulates its activity. p42 ...
... Profilins, regulators of cytoplasmic actin dynamics, also bind to several nuclear proteins but the significance of these interactions is mostly unclear. Here, we describe a novel Myb-related transcription factor, p42POP, as a new ligand for profilin and show that profilin regulates its activity. p42 ...
Bacillus cereus
... Ecology Habitat; symbiosis; contributions to the environment. Pathology Considered non-pathogenic ...
... Ecology Habitat; symbiosis; contributions to the environment. Pathology Considered non-pathogenic ...
Nitrate reductase activity in chicory roots
... The NR level was quantified by the two sites ELISA procedure according to Cherel et al. (1986) using monoclonal anti-NR maize 96925 and S6 polyclonal anti-NR maize antibodies. These antibodies were first tested against chicory root NR by Western blot analysis and immunoprecipitation assays. ...
... The NR level was quantified by the two sites ELISA procedure according to Cherel et al. (1986) using monoclonal anti-NR maize 96925 and S6 polyclonal anti-NR maize antibodies. These antibodies were first tested against chicory root NR by Western blot analysis and immunoprecipitation assays. ...
Extrapolation of elementary rate constants of P
... The basic structural mass action kinetic model is depicted in Fig.1 and Eq 1, 2A and 2B. Important components of the model are: passive permeability across the lipid bilayers obtained in the presence of GF120918; partitioning of substrates into the membranes; active transport by P-gp and by other tr ...
... The basic structural mass action kinetic model is depicted in Fig.1 and Eq 1, 2A and 2B. Important components of the model are: passive permeability across the lipid bilayers obtained in the presence of GF120918; partitioning of substrates into the membranes; active transport by P-gp and by other tr ...
The PRT protein family Sangita C Sinha* and Janet L Smith
... The available structures provide a wealth of information on states of PRTases relevant to catalysis, effectively providing snapshots along the reaction pathway [31,40]. The closed-loop structures, in particular, provide a remarkably consistent picture of Mg•PRPP binding. Mg•PRPP binds in a virtually ...
... The available structures provide a wealth of information on states of PRTases relevant to catalysis, effectively providing snapshots along the reaction pathway [31,40]. The closed-loop structures, in particular, provide a remarkably consistent picture of Mg•PRPP binding. Mg•PRPP binds in a virtually ...
Protein Interaction Technical Handbook
... Usually a combination of techniques is necessary to validate, characterize and confirm protein interactions. Previously unknown proteins may be discovered by their association with one or more proteins that are known. Protein interaction analysis may also uncover unique, unforeseen functional roles ...
... Usually a combination of techniques is necessary to validate, characterize and confirm protein interactions. Previously unknown proteins may be discovered by their association with one or more proteins that are known. Protein interaction analysis may also uncover unique, unforeseen functional roles ...
The LIR motif – crucial for selective autophagy
... Fig. 3. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A) Surface representation of LC3B bound to the p62-LIR peptide (top left), yeast Atg8 bound to the Atg19-LIR peptide (top right), GABARAP-L1 bound to the NBR1-LIR peptide (bottom left) and LC3C bound to the NDP52-LIR ...
... Fig. 3. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A) Surface representation of LC3B bound to the p62-LIR peptide (top left), yeast Atg8 bound to the Atg19-LIR peptide (top right), GABARAP-L1 bound to the NBR1-LIR peptide (bottom left) and LC3C bound to the NDP52-LIR ...
Scientific Opinion on Dietary Reference Values for protein
... Dietary proteins are the source of the nitrogen and indispensable amino acids which the body requires for tissue growth and maintenance. The main pathway of amino acid metabolism is protein synthesis. In this opinion, “protein” is total N x 6.25, and protein requirements are based on nitrogen conten ...
... Dietary proteins are the source of the nitrogen and indispensable amino acids which the body requires for tissue growth and maintenance. The main pathway of amino acid metabolism is protein synthesis. In this opinion, “protein” is total N x 6.25, and protein requirements are based on nitrogen conten ...
Snake venomics of Bothrops punctatus
... was dissolved in 1 mL of water and, after stirring, the absorbance was measured at 228 nm. The percentage of ACE inhibition (% ACEi) was determined using the following formula; % ACEi = (Abs Control − Abs sample)/(Abs control − Abs blank). Control absorbance corresponded to hippuric acid formed afte ...
... was dissolved in 1 mL of water and, after stirring, the absorbance was measured at 228 nm. The percentage of ACE inhibition (% ACEi) was determined using the following formula; % ACEi = (Abs Control − Abs sample)/(Abs control − Abs blank). Control absorbance corresponded to hippuric acid formed afte ...
Biomarker discovery for psychiatric disorders
... 7.2.2.2 Western blot validation of beta galactosidase 7.2.2.3 Pyruvate metabolism alterations in ...
... 7.2.2.2 Western blot validation of beta galactosidase 7.2.2.3 Pyruvate metabolism alterations in ...
An immunoassay for atrazine using tunable immunosorbent Jae-Young Kim, Ashok Mulchandani,
... it is a rapid and simple competitive ELISA method because extensive immobilization, washing, and incubation steps are not necessary. The developed immunoassay exhibited excellent selectivity against various pesticides such as paraoxon, carbaryl, and 2,4-D (data not shown). As expected, cross-reactiv ...
... it is a rapid and simple competitive ELISA method because extensive immobilization, washing, and incubation steps are not necessary. The developed immunoassay exhibited excellent selectivity against various pesticides such as paraoxon, carbaryl, and 2,4-D (data not shown). As expected, cross-reactiv ...
Identification and cDNA cloning of a Xenopus nucleolar
... mitosis by cdc2 kinase (Peter et al., 1990). Serine residues in acidic domains at the amino terminus are likely targets for CK II phosphorylation and TPXKK motifs in basic domains at the amino terminus are probable targets for cdc2 kinase phosphorylation. Phosphorylation of nucleolin by cdc2 kinase ...
... mitosis by cdc2 kinase (Peter et al., 1990). Serine residues in acidic domains at the amino terminus are likely targets for CK II phosphorylation and TPXKK motifs in basic domains at the amino terminus are probable targets for cdc2 kinase phosphorylation. Phosphorylation of nucleolin by cdc2 kinase ...
calcium, kinases and nodulation signalling in legumes
... Ca2+ concentrations at the tip and this establishes a gradient of Ca2+ down the root hair. Adding Nod factor accentuates this gradient23 and induces a wave of Ca2+ that migrates down the shaft of the root-hair cell towards the nucleus26. Isolated regions of high Ca2+ concentrations are observed in t ...
... Ca2+ concentrations at the tip and this establishes a gradient of Ca2+ down the root hair. Adding Nod factor accentuates this gradient23 and induces a wave of Ca2+ that migrates down the shaft of the root-hair cell towards the nucleus26. Isolated regions of high Ca2+ concentrations are observed in t ...
Chloroplast envelope membranes: a dynamic interface between
... membranes and this confers to the envelope membranes a low density. The outer envelope membrane has the highest lipid to protein ratio among plant cells membranes (2.5 to 3 mg lipids/mg proteins), and this is responsible for its very low density (1.08 g/cm3, Block et al. 1983a). The lipid to protein ...
... membranes and this confers to the envelope membranes a low density. The outer envelope membrane has the highest lipid to protein ratio among plant cells membranes (2.5 to 3 mg lipids/mg proteins), and this is responsible for its very low density (1.08 g/cm3, Block et al. 1983a). The lipid to protein ...
PPTX - Bonham Chemistry
... To perform a step mash with an acid rest: – Add hot water to your grain bill to achieve an appropriate rest temperature for an acid rest. Hold the mash at this temperature until the proper pH is achieved . Note this may take more than one hour. – Add hot water infusion or direct heat to raise the ma ...
... To perform a step mash with an acid rest: – Add hot water to your grain bill to achieve an appropriate rest temperature for an acid rest. Hold the mash at this temperature until the proper pH is achieved . Note this may take more than one hour. – Add hot water infusion or direct heat to raise the ma ...
Substrate Specificity and Mechanism from the Structure of
... the first to be solved for any member of this family and reveals a novel nucleotide binding fold and a less common, syn conformation of the glycosidic bond of the ADP in the active site.20 However, determination of the structure of another GHMP kinase family member, mevalonate kinase,16 in complex w ...
... the first to be solved for any member of this family and reveals a novel nucleotide binding fold and a less common, syn conformation of the glycosidic bond of the ADP in the active site.20 However, determination of the structure of another GHMP kinase family member, mevalonate kinase,16 in complex w ...
THE RENAL ELECTROGENIC Na+:HCO3 COTRANSPORTER
... predicted Vm would be either more negative or more positive than the initial Vm, depending on whether the net direction of transport was inward or outward, respectively. However, the application of CO2/HCO3− can cause non-specific Vm changes. In the first place, CO2 diffuses rapidly across most cell ...
... predicted Vm would be either more negative or more positive than the initial Vm, depending on whether the net direction of transport was inward or outward, respectively. However, the application of CO2/HCO3− can cause non-specific Vm changes. In the first place, CO2 diffuses rapidly across most cell ...