Lecture 1 - "Hudel" Luecke
... Amino Acids Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes t ...
... Amino Acids Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes t ...
Pulsing of Membrane Potential in Individual
... experiments, including the ability to control the bioenergetic state through the provision of different substrates, straightforward application of inhibitors, particularly those that cannot be reliably used in vivo due to insufficient uptake by the plant cell, and improved quantitative imaging. This ...
... experiments, including the ability to control the bioenergetic state through the provision of different substrates, straightforward application of inhibitors, particularly those that cannot be reliably used in vivo due to insufficient uptake by the plant cell, and improved quantitative imaging. This ...
Expanding roles of protein kinase CK2 in regulating plant growth
... as other cellular proteins involved in basic physiological processes such as translation and cell cycle. The high degree of redundancy in the genes encoding the catalytic as well as regulatory subunits in plants compared to the animal systems, adds another level of complexity in analysing CK2 functi ...
... as other cellular proteins involved in basic physiological processes such as translation and cell cycle. The high degree of redundancy in the genes encoding the catalytic as well as regulatory subunits in plants compared to the animal systems, adds another level of complexity in analysing CK2 functi ...
A motif of eleven amino acids is a structural adaptation that
... varied in size, which was reflected by the Clin value, NLC and Qmax obtained from each cell were normalized by its Clin. The normalized mean values and standard deviations of the four parameters from six gerbil prestin-expressing cells are shown in Fig. 2C. Using the same voltage protocol, we measur ...
... varied in size, which was reflected by the Clin value, NLC and Qmax obtained from each cell were normalized by its Clin. The normalized mean values and standard deviations of the four parameters from six gerbil prestin-expressing cells are shown in Fig. 2C. Using the same voltage protocol, we measur ...
Chlamydia Exploit the Mammalian Tryptophan-Depletion
... availability since its export depends upon the complex type III secretion system, some ...
... availability since its export depends upon the complex type III secretion system, some ...
BMP binding domains in the extracellular space
... homozygous for a truncated chordin allele lacking the three C-terminal CRs (CR2-4), but retaining CR1 and most of the inter-repeat region, were found to be viable hypomorphic mutants displaying only minor skeletal defects (D. B., J. Klingensmith, J. Rossant and E. M. D. R., unpublished results). Thi ...
... homozygous for a truncated chordin allele lacking the three C-terminal CRs (CR2-4), but retaining CR1 and most of the inter-repeat region, were found to be viable hypomorphic mutants displaying only minor skeletal defects (D. B., J. Klingensmith, J. Rossant and E. M. D. R., unpublished results). Thi ...
as a PDF
... The lactic acid bacteria are mainly studied for their capacities to ferment carbon sources, which results in acidification of the medium. It was therefore surprising to discover that some bacteria, including Lactococcus lactis, had the capacity for respiration-based growth when a heme source and oxy ...
... The lactic acid bacteria are mainly studied for their capacities to ferment carbon sources, which results in acidification of the medium. It was therefore surprising to discover that some bacteria, including Lactococcus lactis, had the capacity for respiration-based growth when a heme source and oxy ...
4. characterisation of novel proteins
... spatially and temporally active only in the developing seed. Data generated over three generations confirmed stability of the introduced genetic elements and indicated that the genes are inherited in a predicted manner according to Mendelian principles. Bioinformatic analysis of the insertion site a ...
... spatially and temporally active only in the developing seed. Data generated over three generations confirmed stability of the introduced genetic elements and indicated that the genes are inherited in a predicted manner according to Mendelian principles. Bioinformatic analysis of the insertion site a ...
Salt tolerance (STO), a stress-related protein, has a major role in
... far-red (FRc, 0.017–0.56 lmol m)2 sec)1) or blue light (Bc, 0.1–10 lmol m)2 sec)1). RNAi and T-DNA lines exhibited a pronounced inhibition of hypocotyl growth compared with the wild type under all the light conditions tested. However, the STO-overexpressing lines, including GFP fusion overexpressors ...
... far-red (FRc, 0.017–0.56 lmol m)2 sec)1) or blue light (Bc, 0.1–10 lmol m)2 sec)1). RNAi and T-DNA lines exhibited a pronounced inhibition of hypocotyl growth compared with the wild type under all the light conditions tested. However, the STO-overexpressing lines, including GFP fusion overexpressors ...
Assembling the Presynaptic Active Zone: A Characterization of an
... 30 min after the initial axodendritic contact (VardinonFriedman et al., 2000; see also Ahmari et al., 2000). In contrast, the recruitment of glutamate receptors, presumably to the postsynaptic compartment, seems to be delayed on average by another 40 min (VardinonFriedman et al., 2000). These studie ...
... 30 min after the initial axodendritic contact (VardinonFriedman et al., 2000; see also Ahmari et al., 2000). In contrast, the recruitment of glutamate receptors, presumably to the postsynaptic compartment, seems to be delayed on average by another 40 min (VardinonFriedman et al., 2000). These studie ...
The Structure of the PanD/PanZ Protein Complex
... Figure 3. NMR Analysis of the PanD-PanZ.AcCoA Complex (A) Overlay of the crystal structure of PanZ in complex with PanD with the NMR solution structure of the PanZ.AcCoA complex (PDB code 1K5T). (B) Stereoscopic view of the CoA-binding site of PanZ showing key hydrogen-bonding interactions. Binding ...
... Figure 3. NMR Analysis of the PanD-PanZ.AcCoA Complex (A) Overlay of the crystal structure of PanZ in complex with PanD with the NMR solution structure of the PanZ.AcCoA complex (PDB code 1K5T). (B) Stereoscopic view of the CoA-binding site of PanZ showing key hydrogen-bonding interactions. Binding ...
Depletion of the co-chaperone CDC-37 reveals two
... elegans. Of the three potential Cdc37 homologs identified by sequence similarity, only one, W08F4.8, was predicted to encode a protein similar in overall size and domain structure to the H. sapiens CDC37 protein (see Fig. S1 in the supplementary material). W08F4.8 protein was found to interact with ...
... elegans. Of the three potential Cdc37 homologs identified by sequence similarity, only one, W08F4.8, was predicted to encode a protein similar in overall size and domain structure to the H. sapiens CDC37 protein (see Fig. S1 in the supplementary material). W08F4.8 protein was found to interact with ...
- Wiley Online Library
... least in part, mediated by TOR (Kapahi et al., 2004; Hansen et al., 2007). Similar observations have also been reported in yeast (Kaeberlein et al., 2005). It has been shown in many organisms that, in response to nutrient or energy deficiency (depletion of amino acid or cellular ATP level), TOR decr ...
... least in part, mediated by TOR (Kapahi et al., 2004; Hansen et al., 2007). Similar observations have also been reported in yeast (Kaeberlein et al., 2005). It has been shown in many organisms that, in response to nutrient or energy deficiency (depletion of amino acid or cellular ATP level), TOR decr ...
The Role of Cyclin B in Meiosis I
... G1 and G2 periods are absent or very short in these "cleavage divisions" presumably because most of the necessary materials have been laid down previously during oogenesis. There is no evidence that other external signals, like growth factors, play any role in controlling the very early cell cycles. ...
... G1 and G2 periods are absent or very short in these "cleavage divisions" presumably because most of the necessary materials have been laid down previously during oogenesis. There is no evidence that other external signals, like growth factors, play any role in controlling the very early cell cycles. ...
Moore BA, Gonzalez Aviles GD, Larkins CE, Hillman MJ, Caspary T. Mamm Genome. 2010 Aug;21(7-8):350-60. Mitochondrial retention of Opa1 is required for mouse embryogenesis
... Opa1 is one of three GTPases known to regulate mitochondrial fusion. When Opa1 is knocked down in cells, mitochondrial fragmentation results (Chen et al. 2005; Cipolat et al. 2004; Griparic et al. 2004; Olichon et al. 2003). To determine whether the mutation we found in Opa1 disrupts Opa1 function, ...
... Opa1 is one of three GTPases known to regulate mitochondrial fusion. When Opa1 is knocked down in cells, mitochondrial fragmentation results (Chen et al. 2005; Cipolat et al. 2004; Griparic et al. 2004; Olichon et al. 2003). To determine whether the mutation we found in Opa1 disrupts Opa1 function, ...
(2009). Age determination and growth in the male South African fur
... on my PhD work. Published six papers based on my work. 1988 to 1989. Research Associate in the Botany Department of the University of British Columbia (UBC), Vancouver, B.C., Canada. I have published a paper based upon some of my joint work while at UBC (Kafkafi et al., 1992). Recently I published a ...
... on my PhD work. Published six papers based on my work. 1988 to 1989. Research Associate in the Botany Department of the University of British Columbia (UBC), Vancouver, B.C., Canada. I have published a paper based upon some of my joint work while at UBC (Kafkafi et al., 1992). Recently I published a ...
DOCTORAL THESIS
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
ribosomal defects in a mutant deficient in the yajl homolog of the
... acyl-CoA dehydrogenase FadE, and proline oxidase PutA, all of which transfer electrons from substrates to quinones without using NAD/NADH (Figure 2). The expression levels of mRNAs coding for respiratory dehydrogenases were determined by DNA array experiments (Figure 3A). Mutant/parental strain gene ...
... acyl-CoA dehydrogenase FadE, and proline oxidase PutA, all of which transfer electrons from substrates to quinones without using NAD/NADH (Figure 2). The expression levels of mRNAs coding for respiratory dehydrogenases were determined by DNA array experiments (Figure 3A). Mutant/parental strain gene ...
Thiele et al.: `Genome-scale reconstruction of E. coli`s transcriptional
... A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine. [43]. Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tR ...
... A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine. [43]. Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tR ...
Generation of ATP during Cytochrome-linked
... hydratase (fumarase), glycerol I -phosphate was omitted from the reaction mixture. After 20 min, the reaction was stopped with 0.8 ml 18 N-H,SO,. After centrifuging succinate and fumarate were measured in the supernatant fluid with the gas chromatograph as described by Holdeman & Moore (1972). Fumar ...
... hydratase (fumarase), glycerol I -phosphate was omitted from the reaction mixture. After 20 min, the reaction was stopped with 0.8 ml 18 N-H,SO,. After centrifuging succinate and fumarate were measured in the supernatant fluid with the gas chromatograph as described by Holdeman & Moore (1972). Fumar ...
The symbiotic ion channel homolog DMI1 is localized in the nuclear
... localization signal (NLS), which targets DMI1 to the nucleus. In order to determine if this domain is essential for DMI1 localization and function, we deleted the 76 amino acids from the N-terminus of DMI1 and introduced a codon for a new initiator methionine. The truncated DMI1 (tDMI1) was fused to ...
... localization signal (NLS), which targets DMI1 to the nucleus. In order to determine if this domain is essential for DMI1 localization and function, we deleted the 76 amino acids from the N-terminus of DMI1 and introduced a codon for a new initiator methionine. The truncated DMI1 (tDMI1) was fused to ...
Systems biology of inborn errors of metabolism
... understanding of the precise biochemical pathology involved. Constraint-based metabolic network reconstruction and modeling is the core systems biology methods to analyze the complex interactions between cellular components that maintain cellular homeostasis. Simultaneously, the global human metabol ...
... understanding of the precise biochemical pathology involved. Constraint-based metabolic network reconstruction and modeling is the core systems biology methods to analyze the complex interactions between cellular components that maintain cellular homeostasis. Simultaneously, the global human metabol ...
as a PDF - CiteSeerX
... full-length CD34, having a relatively short intracellular region of 73 amino acids. An alternatively spliced form with a shorter intracellular tail is expressed at lower abundance. Full-length CD34 is highly phosphorylated by protein kinase C (PKC).5 CD34 itself does not have intrinsic kinase activi ...
... full-length CD34, having a relatively short intracellular region of 73 amino acids. An alternatively spliced form with a shorter intracellular tail is expressed at lower abundance. Full-length CD34 is highly phosphorylated by protein kinase C (PKC).5 CD34 itself does not have intrinsic kinase activi ...
Chapter 4. Unnatural amino acids with caged side chains
... The advent of techniques to alter the amino acids of proteins has permitted the consideration of ‘caged proteins,’ in which particular residues in a protein are provided with photo-removable protecting groups. A number of recent reviews have presented a survey of this literature.21-23 Interesting ex ...
... The advent of techniques to alter the amino acids of proteins has permitted the consideration of ‘caged proteins,’ in which particular residues in a protein are provided with photo-removable protecting groups. A number of recent reviews have presented a survey of this literature.21-23 Interesting ex ...