Amino acid composition of kolomikta actinidia
... A high nutritional value of fruits led to a significant increase in their consumption. Recently, many researchers have been recommending the use of fruits, including kiwifruits (Actinidia chinensis), with a high antioxidant activity (Jung et al., 2005; Scalzo et al., 2005). Of almost 50 species of A ...
... A high nutritional value of fruits led to a significant increase in their consumption. Recently, many researchers have been recommending the use of fruits, including kiwifruits (Actinidia chinensis), with a high antioxidant activity (Jung et al., 2005; Scalzo et al., 2005). Of almost 50 species of A ...
The amoebae plate test implicates a paralogue of lpxB in the
... pneumophila resides in this nutritionally rich compartment, the vacuole may acidify (Sturgill-Koszycki & Swanson, 2000), and bacterial replication apparently proceeds without requiring a functional Icm/Dot transporter (Coers et al., ...
... pneumophila resides in this nutritionally rich compartment, the vacuole may acidify (Sturgill-Koszycki & Swanson, 2000), and bacterial replication apparently proceeds without requiring a functional Icm/Dot transporter (Coers et al., ...
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn
... and we are exceptionally grateful to the numerous authors of oral and poster presentations for their contributions to this conference. We are very thankful to our main sponsors and industrial partners, as well as the German Research Foundation (DFG), the Federation of European Microbiological Societ ...
... and we are exceptionally grateful to the numerous authors of oral and poster presentations for their contributions to this conference. We are very thankful to our main sponsors and industrial partners, as well as the German Research Foundation (DFG), the Federation of European Microbiological Societ ...
The mating pair stabilization protein, TraN, of the F plasmid is an
... importance in TraN function. TraN appears to require both intra- and intermolecular disulfide bond formation for its stability and structure as demonstrated by its instability in a dsbA mutant and its aberrant migration on SDS-polyacrylamide gels under non-reducing conditions or by cross-linking wit ...
... importance in TraN function. TraN appears to require both intra- and intermolecular disulfide bond formation for its stability and structure as demonstrated by its instability in a dsbA mutant and its aberrant migration on SDS-polyacrylamide gels under non-reducing conditions or by cross-linking wit ...
Structure-Based Systematic Isolation of Conditional-Lethal Mutations in the Single Yeast Calmodulin Gene.
... copy, and overexpressed. Whereas all but one of the single mutations caused no obvious phenotype, most of the multiple mutations caused obvious growth phenotypes. Fivewere lethal, 6 were lethal only in synthetic medium, 13 were temperature-sensitive lethal and 2 had no discernible phenotypic consequ ...
... copy, and overexpressed. Whereas all but one of the single mutations caused no obvious phenotype, most of the multiple mutations caused obvious growth phenotypes. Fivewere lethal, 6 were lethal only in synthetic medium, 13 were temperature-sensitive lethal and 2 had no discernible phenotypic consequ ...
Mitochondrial membrane lipid remodeling in
... oxidases (AOX) [4] and type II NAD(P)H dehydrogenases [5], which result in high metabolic plasticity and make plants endowed with a remarkable potential for adaptation. Interestingly, taking into account the widespread taxonomic distribution of AOX, which is also present in prokaryotes and, among eu ...
... oxidases (AOX) [4] and type II NAD(P)H dehydrogenases [5], which result in high metabolic plasticity and make plants endowed with a remarkable potential for adaptation. Interestingly, taking into account the widespread taxonomic distribution of AOX, which is also present in prokaryotes and, among eu ...
Kinetics of MPF and histone H1 kinase activity differ during the G2
... release. This indicates that histone Hi kinase activity in mouse oocytes and biological MPF activity are completely dependent on protein synthesis. However. the difference in the dynamics of the activities between the two might reside in differing half lifes of their active and/or activating princip ...
... release. This indicates that histone Hi kinase activity in mouse oocytes and biological MPF activity are completely dependent on protein synthesis. However. the difference in the dynamics of the activities between the two might reside in differing half lifes of their active and/or activating princip ...
SCARFACE Encodes an ARF-GAP That Is Required for Normal
... Intermediate and Weak sfc Alleles We previously described five phenotypically identical sfc alleles (sfc-1 through sfc-5) (Deyholos et al., 2000). sfc mutants are small, with epinastic cotyledons and leaves that also show distinct vein pattern defects. In contrast with the wild type, which produces ...
... Intermediate and Weak sfc Alleles We previously described five phenotypically identical sfc alleles (sfc-1 through sfc-5) (Deyholos et al., 2000). sfc mutants are small, with epinastic cotyledons and leaves that also show distinct vein pattern defects. In contrast with the wild type, which produces ...
The Serratia gene cluster encoding biosynthesis of the red antibiotic
... genomic contexts of the clusters and their regulation are very different. The Serratia 39006 pig gene cluster contains an additional gene, tentatively designated pigO. RT-PCR and primer extension has confirmed that there is transcriptional readthrough between pigN and pigO (Slater et al., 2003), con ...
... genomic contexts of the clusters and their regulation are very different. The Serratia 39006 pig gene cluster contains an additional gene, tentatively designated pigO. RT-PCR and primer extension has confirmed that there is transcriptional readthrough between pigN and pigO (Slater et al., 2003), con ...
Guanine Nucleotide Exchange-Independent Activation of Gs Protein
... Preincubation time with GTP␥S was 15 min, because our kinetic experiments showed that adenylyl cyclase activation in the presence of GTP␥S reached a peak in 15 min. All of the incubations were at 37°C, and total assay time was 5 min. Time-dependent cAMP accumulation was determined by sampling the re ...
... Preincubation time with GTP␥S was 15 min, because our kinetic experiments showed that adenylyl cyclase activation in the presence of GTP␥S reached a peak in 15 min. All of the incubations were at 37°C, and total assay time was 5 min. Time-dependent cAMP accumulation was determined by sampling the re ...
Active Transport of Fentanyl by the Blood-Brain Barrier1
... To corroborate these findings, a study was done to determine the rates of release of [3H]fentanyl from cells preloaded with [3H]fentanyl under control conditions, and one was done in which the supernatant contained 10 mM cold fentanyl (EC75 for saturating the inward transport mechanism). Figure 2 sh ...
... To corroborate these findings, a study was done to determine the rates of release of [3H]fentanyl from cells preloaded with [3H]fentanyl under control conditions, and one was done in which the supernatant contained 10 mM cold fentanyl (EC75 for saturating the inward transport mechanism). Figure 2 sh ...
Cardiac mitochondrial matrix and respiratory complex - AJP
... et al. (123) and Weiland and colleagues (219, 220), was one of the first examples of metabolic control by enzyme phosphorylation. This discovery and the previously described regulation of GP by protein phosphorylation (114) were two of the early examples of acute modulation of enzyme activity by pro ...
... et al. (123) and Weiland and colleagues (219, 220), was one of the first examples of metabolic control by enzyme phosphorylation. This discovery and the previously described regulation of GP by protein phosphorylation (114) were two of the early examples of acute modulation of enzyme activity by pro ...
Identification of a Vinyl Reductase Gene for
... Arabidopsis polymorphism collection database, and we confirmed that the DVR gene is located on the region covered by the BAC clones shown in Figure 3A. There were four open reading frames (ORFs) encoding putative reductases whose functions were not yet assigned. By sequencing these genes, we finally ...
... Arabidopsis polymorphism collection database, and we confirmed that the DVR gene is located on the region covered by the BAC clones shown in Figure 3A. There were four open reading frames (ORFs) encoding putative reductases whose functions were not yet assigned. By sequencing these genes, we finally ...
The Platform Protein Is Essential for Type IV Pilus
... forces that drive twitching arise from the depolymerization of surface-attached pili and consequent translocation of the cell body toward the point of pilus attachment (8 –11). Four interdependent subcomplexes are proposed to form the apparatus responsible for T4P and T2S biogenesis and function (7) ...
... forces that drive twitching arise from the depolymerization of surface-attached pili and consequent translocation of the cell body toward the point of pilus attachment (8 –11). Four interdependent subcomplexes are proposed to form the apparatus responsible for T4P and T2S biogenesis and function (7) ...
invited review - Luzimar Teixeira
... In the early 1980s studies in rat adipose cells demonstrated that a major mechanism for the insulin-induced increase in glucose transport was through stimulating the movement of glucose transporter proteins from an intracellular ‘‘microsomal’’ compartment to the plasma membrane (19, 114). Despite it ...
... In the early 1980s studies in rat adipose cells demonstrated that a major mechanism for the insulin-induced increase in glucose transport was through stimulating the movement of glucose transporter proteins from an intracellular ‘‘microsomal’’ compartment to the plasma membrane (19, 114). Despite it ...
PEGylation of Biopharmaceuticals: A Review of Chemistry and
... Modification of biopharmaceutical molecules by covalent conjugation of polyethylene glycol (PEG) molecules is known to enhance pharmacologic and pharmaceutical properties of proteins and other large molecules and has been used successfully in 12 approved drugs. Both linear and branched-chain PEG reag ...
... Modification of biopharmaceutical molecules by covalent conjugation of polyethylene glycol (PEG) molecules is known to enhance pharmacologic and pharmaceutical properties of proteins and other large molecules and has been used successfully in 12 approved drugs. Both linear and branched-chain PEG reag ...
Cellulose Biosynthesis in Oomycetes
... This thesis is based on work on several organisms and spanning a broad spectrum of techniques. The common thread is two of the most important carbohydrates on earth, namely cellulose and chitin. The aim of the present investigation is to understand the mecha ...
... This thesis is based on work on several organisms and spanning a broad spectrum of techniques. The common thread is two of the most important carbohydrates on earth, namely cellulose and chitin. The aim of the present investigation is to understand the mecha ...
Unveiling the physiological and molecular basis of Mycobacterium
... non-replicating state of infecting microorganisms that enables evasion from the host’s immune system. The clinical phenotype of TB thus raises the question of whether or not M. tuberculosis latent infections are associated to the biofilm phenotype in vivo and if these multicellular structures may al ...
... non-replicating state of infecting microorganisms that enables evasion from the host’s immune system. The clinical phenotype of TB thus raises the question of whether or not M. tuberculosis latent infections are associated to the biofilm phenotype in vivo and if these multicellular structures may al ...
The yeast Saccharomyces cerevisiae– the main
... CIP process. Traditional agar plating techniques using selective cultivation media, such as WL actidione agar (for aerobic bacteria), Raka Ray for anaerobic bacteria and lysine (as a sole nitrogen source) agar for selecting non-Saccharomyces wild yeast (Walker, 1998; Campbell, 2003), are still in us ...
... CIP process. Traditional agar plating techniques using selective cultivation media, such as WL actidione agar (for aerobic bacteria), Raka Ray for anaerobic bacteria and lysine (as a sole nitrogen source) agar for selecting non-Saccharomyces wild yeast (Walker, 1998; Campbell, 2003), are still in us ...
ftsZ mutations affecting cell division frequency, placement and
... A key event in cytokinesis in bacteria is the assembly of the essential division protein FtsZ into ring-like structures at the nascent division site. FtsZ is the prokaryotic homologue of tubulin, and is found in nearly all bacteria. In vitro, FtsZ polymerizes in the presence of GTP to form higher-or ...
... A key event in cytokinesis in bacteria is the assembly of the essential division protein FtsZ into ring-like structures at the nascent division site. FtsZ is the prokaryotic homologue of tubulin, and is found in nearly all bacteria. In vitro, FtsZ polymerizes in the presence of GTP to form higher-or ...
Complement Levels and Activity in the Normal and LPS - AJP-Lung
... BAL levels of the different proteins in the normal lung appear to differ from levels in human serum (Reynolds and Newball 1974;Robertson, et al. 1976;Watford, et al. 2000). Complement activity has also been observed in the lung. Classical pathway activity was measured in human BAL using standard in ...
... BAL levels of the different proteins in the normal lung appear to differ from levels in human serum (Reynolds and Newball 1974;Robertson, et al. 1976;Watford, et al. 2000). Complement activity has also been observed in the lung. Classical pathway activity was measured in human BAL using standard in ...
ABSTRACT Title of Document:
... Escherichia coli as commensal and model pathogen ............................................... 2 Extraintestinal Pathogenic Escherichia coli (ExPEC).............................................. 3 Virulence factors associated with ExPECs ............................................................ ...
... Escherichia coli as commensal and model pathogen ............................................... 2 Extraintestinal Pathogenic Escherichia coli (ExPEC).............................................. 3 Virulence factors associated with ExPECs ............................................................ ...
9 The AMP-activated protein kinase: more than an energy sensor
... via phosphorylation of its Thr172 by LKB1. Conditions leading to changes in AMP concentration are directly related to changes in ATP concentrations. At any time, intracellular ATP concentrations reflect the balance between energy supply-and-demand. In most cells, ATP supply relies on mitochondrial o ...
... via phosphorylation of its Thr172 by LKB1. Conditions leading to changes in AMP concentration are directly related to changes in ATP concentrations. At any time, intracellular ATP concentrations reflect the balance between energy supply-and-demand. In most cells, ATP supply relies on mitochondrial o ...