Susan Viggiano and Michael S. Cosgrove
... suggest different family members are controlled by distinct regulatory mechanisms. For example, although the MLL1 core complex has predominantly mono- and dimethyltransferase activity in vitro (36), a similar complex assembled with MLL3 shows only monomethylation activity (37, 38). Indeed, MLL2/3 co ...
... suggest different family members are controlled by distinct regulatory mechanisms. For example, although the MLL1 core complex has predominantly mono- and dimethyltransferase activity in vitro (36), a similar complex assembled with MLL3 shows only monomethylation activity (37, 38). Indeed, MLL2/3 co ...
Eukaryote-Like Serine/Threonine Kinases and Phosphatases in
... maintained across kingdoms (Fig. 1B) (also see “Structural and functional studies of bacterial eSTKs”). While subdomains vary in size, there is little sequence homology between members of the superfamily. However, the kinase catalytic domain can be defined by the presence of specific conserved motif ...
... maintained across kingdoms (Fig. 1B) (also see “Structural and functional studies of bacterial eSTKs”). While subdomains vary in size, there is little sequence homology between members of the superfamily. However, the kinase catalytic domain can be defined by the presence of specific conserved motif ...
Functional Interactions Between the Subunits of the Lactose
... (123)). For most of these transporters, it remains to be determined whether functional interactions between subunits take place. Furthermore, in those cases where cooperativity between subunits has been shown, the role of the oligomeric structure in the mechanism of transport is still far from clear ...
... (123)). For most of these transporters, it remains to be determined whether functional interactions between subunits take place. Furthermore, in those cases where cooperativity between subunits has been shown, the role of the oligomeric structure in the mechanism of transport is still far from clear ...
Selenocysteine insertion directed by the 3′
... expressed from the construct containing a mutant SECIS element showed a very weak 75Se signal (Figure 2, lane 2, upper panel), suggesting that the functional SECIS element was required for Sec insertion at the UGA codon. However, when the UGA codon was replaced with UAA (independently of whether the ...
... expressed from the construct containing a mutant SECIS element showed a very weak 75Se signal (Figure 2, lane 2, upper panel), suggesting that the functional SECIS element was required for Sec insertion at the UGA codon. However, when the UGA codon was replaced with UAA (independently of whether the ...
Barley Aleurone Cells Contain Two Types of
... which we have called secondary vacuoles. Differential interference contrast (DIC) microscopy showed that these two types of vacuole occur in both GA- and ABA-treated aleurone protoplasts (Figure 1). In Figure 1, the plane of focus was selected to most clearly reveal the characteristics of the second ...
... which we have called secondary vacuoles. Differential interference contrast (DIC) microscopy showed that these two types of vacuole occur in both GA- and ABA-treated aleurone protoplasts (Figure 1). In Figure 1, the plane of focus was selected to most clearly reveal the characteristics of the second ...
mexCD-oprJ aeruginosa
... repressor of mexCD-oprJ with homology to NfxB, EsrC, was discovered. In the absence of CHXmediated envelope stress, the expression of esrC is negatively regulated by NfxB. In the presence of CHX-mediated envelope stress, the repressor activity of NfxB is alleviated and EsrC is produced, where it con ...
... repressor of mexCD-oprJ with homology to NfxB, EsrC, was discovered. In the absence of CHXmediated envelope stress, the expression of esrC is negatively regulated by NfxB. In the presence of CHX-mediated envelope stress, the repressor activity of NfxB is alleviated and EsrC is produced, where it con ...
Phosphorylation of the F1Fo ATP Synthase Я Subunit
... with respect to growth, complex and supercomplex formation, and enzymatic activity (ATPase rate). The most striking mutant was the T262 site, for which the phosphomimetic (T262E) abolished activity, whereas the nonphosphorylatable strain (T262A) had an ATPase rate equivalent to wild type. Although T ...
... with respect to growth, complex and supercomplex formation, and enzymatic activity (ATPase rate). The most striking mutant was the T262 site, for which the phosphomimetic (T262E) abolished activity, whereas the nonphosphorylatable strain (T262A) had an ATPase rate equivalent to wild type. Although T ...
Active Transport of the Survival Motor Neuron Protein and
... Spinal muscular atrophy (SMA) is a neurodegenerative disease caused by deletion and/or mutation of the survival motor neuron protein Gene (SMN1) that results in the expression of a truncated protein lacking the C terminal exon-7. Whereas SMN has been shown to be an important component of diverse rib ...
... Spinal muscular atrophy (SMA) is a neurodegenerative disease caused by deletion and/or mutation of the survival motor neuron protein Gene (SMN1) that results in the expression of a truncated protein lacking the C terminal exon-7. Whereas SMN has been shown to be an important component of diverse rib ...
Capping protein: new insights into mechanism
... S100B binds tightly to the 12- and 28-residue a-subunit peptides and to denatured CP [26,28]. Relatively high concentrations of the non-ionic detergent Triton-X100 did allow S100B to bind weakly to CP without denaturing either protein, however, and this binding inhibited the capping activity of CP i ...
... S100B binds tightly to the 12- and 28-residue a-subunit peptides and to denatured CP [26,28]. Relatively high concentrations of the non-ionic detergent Triton-X100 did allow S100B to bind weakly to CP without denaturing either protein, however, and this binding inhibited the capping activity of CP i ...
FAT/CD36 is located on the outer mitochondrial membrane
... function in FAT/CD36-KO mice, a recent investigation has also failed to detect FAT/CD36 on mitochondrial membranes utilizing both immunoblotting and fluorescence immunocytochemistry techniques [21]. Indeed, contamination of other cell fractions has been an issue when isolating skeletal muscle mitoch ...
... function in FAT/CD36-KO mice, a recent investigation has also failed to detect FAT/CD36 on mitochondrial membranes utilizing both immunoblotting and fluorescence immunocytochemistry techniques [21]. Indeed, contamination of other cell fractions has been an issue when isolating skeletal muscle mitoch ...
Actin and Myosin Function in Directed Vacuole Movement during
... Affinity purification of anti-actin antibody was performed with nitrocellulose blots as described (39). Simultaneous visualization of Myo2p and the vacuole membrane was performed in yeast zygotes. 2.5 OD600 U each of mid-log phase M A T a and M A T a strains were mixed together in YEPD medium and sh ...
... Affinity purification of anti-actin antibody was performed with nitrocellulose blots as described (39). Simultaneous visualization of Myo2p and the vacuole membrane was performed in yeast zygotes. 2.5 OD600 U each of mid-log phase M A T a and M A T a strains were mixed together in YEPD medium and sh ...
Zinc transporters that regulate vacuolar zinc storage in
... strain. The resulting zrt3 mutant was viable in both rich (YPD) and minimal synthetic de®ned (SD) media, indicating that Zrt3p is not required for growth under standard laboratory conditions. To test whether Zrt3p is important under zinc limitation, growth of wild-type and zrt3 mutant strains was ex ...
... strain. The resulting zrt3 mutant was viable in both rich (YPD) and minimal synthetic de®ned (SD) media, indicating that Zrt3p is not required for growth under standard laboratory conditions. To test whether Zrt3p is important under zinc limitation, growth of wild-type and zrt3 mutant strains was ex ...
- VU Research Repository
... One of the major yeast stressors during fermentation is ethanol accumulation. Ethanol stress is associated with reduced cell growth and viability, consequently lowering yeast productivity. Although the underlying causes of ethanol inhibition of cells are yet to be identified, it has been discovered ...
... One of the major yeast stressors during fermentation is ethanol accumulation. Ethanol stress is associated with reduced cell growth and viability, consequently lowering yeast productivity. Although the underlying causes of ethanol inhibition of cells are yet to be identified, it has been discovered ...
Some Aspects of Fatty Acid Oxidation in Isolated Fat
... male Sprague-Dawley rats from Bantin and Kingman (Hull, U.K.) or were Wistar or Sprague-Dawley rats bred in the animal colony at University College London. Fed animals were maintained on cube diet 41B (Bruce & Parkes, 1949) and weighed 140-200g. Starved animals were within this weight range at the t ...
... male Sprague-Dawley rats from Bantin and Kingman (Hull, U.K.) or were Wistar or Sprague-Dawley rats bred in the animal colony at University College London. Fed animals were maintained on cube diet 41B (Bruce & Parkes, 1949) and weighed 140-200g. Starved animals were within this weight range at the t ...
Mitochondrial trafficking and anchoring in neurons
... et al., 2005; Russo et al., 2009), as loss of dMiro impairs both kinesin- and dynein-driven transport and overexpressing dMiro also alters mitochondrial transport in both directions. These findings support an attractive model in which the relative activity of the opposite-moving motors is regulated ...
... et al., 2005; Russo et al., 2009), as loss of dMiro impairs both kinesin- and dynein-driven transport and overexpressing dMiro also alters mitochondrial transport in both directions. These findings support an attractive model in which the relative activity of the opposite-moving motors is regulated ...
Granska/Öppna
... identified widely, for example, in the capillary endothelial cells of the brain (bloodbrain barrier), intestine, kidney, liver, testes and placenta (blood-placental barrier) (Thiebaut et al. 1987, Cordon-Cardo et al. 1989). Many of these tissues are extremely important for the absorption, distributi ...
... identified widely, for example, in the capillary endothelial cells of the brain (bloodbrain barrier), intestine, kidney, liver, testes and placenta (blood-placental barrier) (Thiebaut et al. 1987, Cordon-Cardo et al. 1989). Many of these tissues are extremely important for the absorption, distributi ...
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen
... The flux through a pathway or an enzyme can be changed by many different regulatory mechanisms. Speaking in the terms introduced above, many buttons are available in the cell. In this section I will describe the various levels of regulation briefly. The regulatory mechanisms can be dissected crudely ...
... The flux through a pathway or an enzyme can be changed by many different regulatory mechanisms. Speaking in the terms introduced above, many buttons are available in the cell. In this section I will describe the various levels of regulation briefly. The regulatory mechanisms can be dissected crudely ...
The Plant Cell - Department of Biology
... dimerization and the transport of cargo, respectively. Recent completion of the genome sequences of several eukaryotes, ranging from a simple eukaryote to highly evolved multicellular organisms, has allowed the identification of a large number of kinesins in an organism. For example, 45 kinesin gene ...
... dimerization and the transport of cargo, respectively. Recent completion of the genome sequences of several eukaryotes, ranging from a simple eukaryote to highly evolved multicellular organisms, has allowed the identification of a large number of kinesins in an organism. For example, 45 kinesin gene ...
Transmembrane Movement of Exogenous Long
... between fatty acid in a bound form and a free form. The bound form of the fatty acid may be part of a mixed micelle, as would be case in the intestine or in a protein-bound form as in a capillary bed. Step 5 is linked to the intracellular metabolism, which drives this process forward. This may invol ...
... between fatty acid in a bound form and a free form. The bound form of the fatty acid may be part of a mixed micelle, as would be case in the intestine or in a protein-bound form as in a capillary bed. Step 5 is linked to the intracellular metabolism, which drives this process forward. This may invol ...
C-terminal processing of yeast Spt7 occurs in the absence of
... Based upon the mobility of this form and the differential function of Spt7 derivatives with C-terminal deletions, Spt7SLIK is predicted to contain a C-terminal truncation of approximately 200 amino acids [4,6]. To detect these forms of Spt7 we engineered a centromeric plasmid expressing a TAP and Fl ...
... Based upon the mobility of this form and the differential function of Spt7 derivatives with C-terminal deletions, Spt7SLIK is predicted to contain a C-terminal truncation of approximately 200 amino acids [4,6]. To detect these forms of Spt7 we engineered a centromeric plasmid expressing a TAP and Fl ...
p23 and organization of the Golgi apparatus
... Belden and Barlowe, 1996). Until now, no specific association between a p24 protein and a cargo molecule has been reported. However, it is possible that p24-cargo interactions are weak and/or transient, and cannot be revealed by classical biochemical approaches. Alternatively, it is possible that p2 ...
... Belden and Barlowe, 1996). Until now, no specific association between a p24 protein and a cargo molecule has been reported. However, it is possible that p24-cargo interactions are weak and/or transient, and cannot be revealed by classical biochemical approaches. Alternatively, it is possible that p2 ...
Structure, mechanism and regulation of pyruvate carboxylase
... gluconeogenic enzymes, glucose 6-phosphatase and fructose 1,6bisphosphatase, are not present in adipose tissue, Ballard and Hanson [21] proposed the involvement of PC in the pathway of de novo fatty acid synthesis. PC participates in this pathway by providing oxaloacetate for conversion into citrate ...
... gluconeogenic enzymes, glucose 6-phosphatase and fructose 1,6bisphosphatase, are not present in adipose tissue, Ballard and Hanson [21] proposed the involvement of PC in the pathway of de novo fatty acid synthesis. PC participates in this pathway by providing oxaloacetate for conversion into citrate ...
Relationship between expression of serendipity and
... strain uncovering the nullo gene was provided by Dr E. Wieschaus. Molecular characterization of the D. pseudoobscura and D. subobscura sry clones All molecular methods described in this and other sections were carried out using standard techniques described in Sambrook et al. (1989). M. Aguadé and C ...
... strain uncovering the nullo gene was provided by Dr E. Wieschaus. Molecular characterization of the D. pseudoobscura and D. subobscura sry clones All molecular methods described in this and other sections were carried out using standard techniques described in Sambrook et al. (1989). M. Aguadé and C ...