Exploring the directionality of Escherichia coli formate
... The possibility that FHL could be energy conserving has also led to hypotheses on the origins of life itself. One compelling idea is that the FHL ‘reverse’ reaction may once have dominated early cellular life, coupling the proton motive force to H2-dependent CO2 fixation (Nitschke and Russell 2009) ...
... The possibility that FHL could be energy conserving has also led to hypotheses on the origins of life itself. One compelling idea is that the FHL ‘reverse’ reaction may once have dominated early cellular life, coupling the proton motive force to H2-dependent CO2 fixation (Nitschke and Russell 2009) ...
The Genera Staphylococcus and Macrococcus
... possibility of false-negative reactions due to PCR inhibitors that might be present in some foods. Several conventional procedures for isolating S. aureus from foods are described below. Nonselective Enrichment Procedures It is often necessary to use nonselective enrichment procedures for the detect ...
... possibility of false-negative reactions due to PCR inhibitors that might be present in some foods. Several conventional procedures for isolating S. aureus from foods are described below. Nonselective Enrichment Procedures It is often necessary to use nonselective enrichment procedures for the detect ...
Euglena gracilis ascorbate peroxidase forms an intramolecular
... adopted a PCR-based oligo-capped method utilizing primers corresponding to sequences of an EST clone and a partial cDNA isolated previously [19]. The final clone containing the full-length cDNA, as indicated by the presence of a 5 -TTTTTTTTCG-3 spliced leader at the 5 end [20], was 2403 bp long w ...
... adopted a PCR-based oligo-capped method utilizing primers corresponding to sequences of an EST clone and a partial cDNA isolated previously [19]. The final clone containing the full-length cDNA, as indicated by the presence of a 5 -TTTTTTTTCG-3 spliced leader at the 5 end [20], was 2403 bp long w ...
Investigation of Factors Affecting Opalescence and Phase
... opalescence for a DVD-IgTM protein solution. Larger size and increased asymmetry of the molecule (with respect to shape and surface charge distribution) results in many fold increase in formulation challenges for DVD-IgTM protein. We first established the thermodynamic basis for phase separation and ...
... opalescence for a DVD-IgTM protein solution. Larger size and increased asymmetry of the molecule (with respect to shape and surface charge distribution) results in many fold increase in formulation challenges for DVD-IgTM protein. We first established the thermodynamic basis for phase separation and ...
Biogenesis and multifaceted roles of outer membrane
... found to release spherical membrane-bound vesicles which were produced by pinching off of the OM (Chatterjee & Das, 1967). It was also observed that the density of some granular material was higher near the site where the ‘pinching off’ event was taking place on the OM, and some granules were enteri ...
... found to release spherical membrane-bound vesicles which were produced by pinching off of the OM (Chatterjee & Das, 1967). It was also observed that the density of some granular material was higher near the site where the ‘pinching off’ event was taking place on the OM, and some granules were enteri ...
The Drosophila Sec7 domain guanine nucleotide exchange factor
... (Kraut et al., 2001) encodes a GEF for ARF family G-proteins involved in vesicle trafficking (Fig. 2). garz is a member of a conserved family of ARF-GEFs with homologs in humans (GBF1; 43% identity) (Claude et al., 1999), Caenorhabditis elegans (GBF1; 34% identity; WormBase), Arabidopsis thaliana (G ...
... (Kraut et al., 2001) encodes a GEF for ARF family G-proteins involved in vesicle trafficking (Fig. 2). garz is a member of a conserved family of ARF-GEFs with homologs in humans (GBF1; 43% identity) (Claude et al., 1999), Caenorhabditis elegans (GBF1; 34% identity; WormBase), Arabidopsis thaliana (G ...
Identification and characterization of novel interaction
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
Electron Transport Chains of Lactic Acid Bacteria
... Most bacteria have a proton driven F1F0 ATPase complex to generate ATP, although some bacteria (and Archaea), such as Acetobacterium woodii, have sodium driven ATPase systems (23, 41). LAB also require the F1F0 ATPase for growth, although they, when fermenting, do not use this protein complex to gen ...
... Most bacteria have a proton driven F1F0 ATPase complex to generate ATP, although some bacteria (and Archaea), such as Acetobacterium woodii, have sodium driven ATPase systems (23, 41). LAB also require the F1F0 ATPase for growth, although they, when fermenting, do not use this protein complex to gen ...
Analysis of the genetic potential of the spongederived fungus
... Ircinia fasciculata in the course of a research program focused on the discovery and characterization of novel natural products. This led to the isolation and characterisation of two novel and structurally highly similar polyketides: sorbicillactone A and sorbicillactone B. Consistent with their str ...
... Ircinia fasciculata in the course of a research program focused on the discovery and characterization of novel natural products. This led to the isolation and characterisation of two novel and structurally highly similar polyketides: sorbicillactone A and sorbicillactone B. Consistent with their str ...
A heritable switch in carbon source utilization driven
... gene showed a detectable difference between [gar!] cells and [GAR+] cells on our arrays, but that gene was very strongly affected. Hexose Transporter 3 (HXT3) was ;36fold down-regulated in [GAR+] cells compared with [gar!] cells (Supplemental Fig. S4). No other transcript exhibited more than a twofo ...
... gene showed a detectable difference between [gar!] cells and [GAR+] cells on our arrays, but that gene was very strongly affected. Hexose Transporter 3 (HXT3) was ;36fold down-regulated in [GAR+] cells compared with [gar!] cells (Supplemental Fig. S4). No other transcript exhibited more than a twofo ...
Organization of Physical Interactomes as
... complex schemas are organized in terms of their lower-order constituents. The uncovered schemas span a wide range of cellular activities, with many signaling and transport related higher-order schemas. We establish the functional importance of the schemas by showing that they correspond to functiona ...
... complex schemas are organized in terms of their lower-order constituents. The uncovered schemas span a wide range of cellular activities, with many signaling and transport related higher-order schemas. We establish the functional importance of the schemas by showing that they correspond to functiona ...
PIN-Dependent Auxin Transport: Action, Regulation
... members of the family, and look at the family from an evolutionary perspective. Next, we cover the cell biological and molecular aspects of PIN function, in particular the establishment of their polar subcellular localization. Hormonal and environmental inputs into the regulation of PIN action are s ...
... members of the family, and look at the family from an evolutionary perspective. Next, we cover the cell biological and molecular aspects of PIN function, in particular the establishment of their polar subcellular localization. Hormonal and environmental inputs into the regulation of PIN action are s ...
The Role of the N-Terminal Domains of Bacterial Initiator DnaA in
... 2. Bacterial DnaA—General Overview of the Structure and Function To form a bacterial initiation complex, often called an orisome, DnaA binds to DNA at oriC and employs protein-protein interactions between protomers to assemble into a helical filament that is capable of opening double-stranded DNA (d ...
... 2. Bacterial DnaA—General Overview of the Structure and Function To form a bacterial initiation complex, often called an orisome, DnaA binds to DNA at oriC and employs protein-protein interactions between protomers to assemble into a helical filament that is capable of opening double-stranded DNA (d ...
PKU
... 6 mg/dl may have babies with severe problems. The babies are born smaller than expected with small heads. The babies do not develop normally. Some of the babies have life-threatening heart problems. The clinic will work closely with all young women, when that time comes, to help them have a healthy ...
... 6 mg/dl may have babies with severe problems. The babies are born smaller than expected with small heads. The babies do not develop normally. Some of the babies have life-threatening heart problems. The clinic will work closely with all young women, when that time comes, to help them have a healthy ...
Influence of the regulatory protein RsmA on cellular
... 2004; Laskowski et al., 2004). Transcriptome profiling of the P. aeruginosa PAK retS mutant revealed a decrease in the expression of genes involved in type III secretion and virulence and an increase in the biosynthetic genes (the psl and pel operons) involved in the formation of the mannose- and gl ...
... 2004; Laskowski et al., 2004). Transcriptome profiling of the P. aeruginosa PAK retS mutant revealed a decrease in the expression of genes involved in type III secretion and virulence and an increase in the biosynthetic genes (the psl and pel operons) involved in the formation of the mannose- and gl ...
Biodegradation of Melamine and Cyanuric Acid by a Newly
... in the MEL1 strain was compared with the A. citrulli NRRL B-12227 strain. As shown in Table 1, the specific activities of the melamine deaminase in these strains were similar. The melamine deaminase in the MEL1 strain was also purified and compared with that in the NRRL B-12227 strain. The specific ...
... in the MEL1 strain was compared with the A. citrulli NRRL B-12227 strain. As shown in Table 1, the specific activities of the melamine deaminase in these strains were similar. The melamine deaminase in the MEL1 strain was also purified and compared with that in the NRRL B-12227 strain. The specific ...
Interactions of Annexins with the mu Subunits of the Clathrin
... To search for proteins that interact with the N-terminal domain of annexin A2, the N-terminal 34 residues were fused to the yeast GAL4 DNA binding domain and used as a bait in a yeast two-hybrid screen of a human fetal brain cDNA library. The bait construct was found not to cause autoactivation in c ...
... To search for proteins that interact with the N-terminal domain of annexin A2, the N-terminal 34 residues were fused to the yeast GAL4 DNA binding domain and used as a bait in a yeast two-hybrid screen of a human fetal brain cDNA library. The bait construct was found not to cause autoactivation in c ...
Minireview: Lipid Droplets in Lipogenesis and Lipolysis
... observation that hormone-stimulated lipolysis in these mice was reduced. Thus, in addition to keeping lipases at bay under basal conditions, perilipin appears to coordinate the recruitment and/or activation of lipases under lipolytic conditions, as discussed below. Tansey and colleagues (37) noted t ...
... observation that hormone-stimulated lipolysis in these mice was reduced. Thus, in addition to keeping lipases at bay under basal conditions, perilipin appears to coordinate the recruitment and/or activation of lipases under lipolytic conditions, as discussed below. Tansey and colleagues (37) noted t ...
REVIEWS - Unisciel
... Within the Z-line, titin interacts with other Zline proteins. The two amino-terminal Ig domains of Z-line titin contain a binding site for telethonin (also known as T-cap), which is a small protein that is involved in cellular signalling mechanisms 24,30. Adjacent to the telethonin/T-cap site, there ...
... Within the Z-line, titin interacts with other Zline proteins. The two amino-terminal Ig domains of Z-line titin contain a binding site for telethonin (also known as T-cap), which is a small protein that is involved in cellular signalling mechanisms 24,30. Adjacent to the telethonin/T-cap site, there ...
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)
... Botulism, a rare but life threatening, mainly food borne disease caused by a toxic bacterial protein Botulinum (BoNT)1, well known neurotoxin expressed in an anaerobic spore forming bacteria Clostridium botulinum. Human neurotoxin BoNT elicits flaccid paralysis by a very unique mechanism which inclu ...
... Botulism, a rare but life threatening, mainly food borne disease caused by a toxic bacterial protein Botulinum (BoNT)1, well known neurotoxin expressed in an anaerobic spore forming bacteria Clostridium botulinum. Human neurotoxin BoNT elicits flaccid paralysis by a very unique mechanism which inclu ...
View/Open - eDiss - Georg-August
... shedding is well documented in animals, where it fulfils diverse regulatory functions on a range of different proteins. In plants, ectodomain shedding has so far only been reported for CERK1 and the function of CERK1 ectodomain shedding is unknown. Some evidence for a role in cell death control come ...
... shedding is well documented in animals, where it fulfils diverse regulatory functions on a range of different proteins. In plants, ectodomain shedding has so far only been reported for CERK1 and the function of CERK1 ectodomain shedding is unknown. Some evidence for a role in cell death control come ...
A Receptor-Like Kinase Mediates Ammonium
... we established a set of CAP1-suppression transgenic lines using RNA interference (RNAi). The independent transgenic plants (CAP1-RNAi1, 2, or 3 lines) had distinctly fewer root hairs and abnormally swollen bases compared with the wild type (Supplemental Figure 2C), confirming that deficient expression ...
... we established a set of CAP1-suppression transgenic lines using RNA interference (RNAi). The independent transgenic plants (CAP1-RNAi1, 2, or 3 lines) had distinctly fewer root hairs and abnormally swollen bases compared with the wild type (Supplemental Figure 2C), confirming that deficient expression ...