BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... Permanently denatured [125I]RCMLA or unfolded β-gal was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addit ...
... Permanently denatured [125I]RCMLA or unfolded β-gal was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addit ...
Annexin-Phospholipid Interactions. Functional Implications
... conserved core composed of four (eight in annexin A6) homologous domains of about 70 amino acids showing a similar three-dimensional structure highly conserved throughout annexin evolution [6]. Inside each domain, a sequence of 17 amino acid residues is found which presents an even higher degree of ...
... conserved core composed of four (eight in annexin A6) homologous domains of about 70 amino acids showing a similar three-dimensional structure highly conserved throughout annexin evolution [6]. Inside each domain, a sequence of 17 amino acid residues is found which presents an even higher degree of ...
Protein phosphatase 1 down regulates ZYG
... Because we had identified a mutation in I-2szy-2 as a suppressor of zyg-1(it25), we speculated that loss of additional PP1 regulatory proteins may have a similar effect. To test this hypothesis we identified seven C. elegans proteins which show a high degree of conservation with human PP1 regulato ...
... Because we had identified a mutation in I-2szy-2 as a suppressor of zyg-1(it25), we speculated that loss of additional PP1 regulatory proteins may have a similar effect. To test this hypothesis we identified seven C. elegans proteins which show a high degree of conservation with human PP1 regulato ...
Biochemical characterization of the initial steps of the Kennedy
... two most abundant phospholipid species in eukaryotic cells and many bacteria [1]. In the parasitic protozoan Trypanosoma brucei, the agent of African sleeping sickness, GPCho accounts for approximately half of all phospholipids in both life-cycle stages, whereas GPEtn forms between 16 and 21 % [2]. ...
... two most abundant phospholipid species in eukaryotic cells and many bacteria [1]. In the parasitic protozoan Trypanosoma brucei, the agent of African sleeping sickness, GPCho accounts for approximately half of all phospholipids in both life-cycle stages, whereas GPEtn forms between 16 and 21 % [2]. ...
Presentation - European Bioinformatics Institute
... The contributes_to qualifier • Where an individual gene product that is part of a complex can be annotated to terms that describe the action (function or process) of the whole complex • contributes_to is not needed to annotate a catalytic subunit. Furthermore, contributes_to may be used for any non ...
... The contributes_to qualifier • Where an individual gene product that is part of a complex can be annotated to terms that describe the action (function or process) of the whole complex • contributes_to is not needed to annotate a catalytic subunit. Furthermore, contributes_to may be used for any non ...
Regulation of mitochondrial calcium in plants versus animals
... Plant and animal cells share a need to adjust their physiology rapidly. This is particularly important to deal with changes in their environment, but also to support developmental programmes. Intracellular Ca2+ transients are an essential and universal signalling mechanism for mediating physiologica ...
... Plant and animal cells share a need to adjust their physiology rapidly. This is particularly important to deal with changes in their environment, but also to support developmental programmes. Intracellular Ca2+ transients are an essential and universal signalling mechanism for mediating physiologica ...
Hormone sensitive lipase: structure, function and
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
Axonal of Kinesin in the Chain Isoforms
... olism (Brady, 1985a). Although detailed descriptions exist of the composition and rates of movement for the major axonal transport rate components, less has been known about underlying molecular mechanisms. Discovery of the mechanochemical ATPase kinesin (Brady, 1985b; Vale et al., 1985) provided a ...
... olism (Brady, 1985a). Although detailed descriptions exist of the composition and rates of movement for the major axonal transport rate components, less has been known about underlying molecular mechanisms. Discovery of the mechanochemical ATPase kinesin (Brady, 1985b; Vale et al., 1985) provided a ...
Exploitation of Bile Acid Transport Systems in Prodrug Design
... stoichiometry of Na+:bile acid is identified of being 2:1 [54]. The second uptake system is Na+independent and involves several members of the organic anion transporting polypeptide (OATP) family. Three OATPs, which are expressed in the liver and accept bile salts as substrates, have been cloned. Th ...
... stoichiometry of Na+:bile acid is identified of being 2:1 [54]. The second uptake system is Na+independent and involves several members of the organic anion transporting polypeptide (OATP) family. Three OATPs, which are expressed in the liver and accept bile salts as substrates, have been cloned. Th ...
University of Groningen The functional relationship between
... intestinal lumen. The bile salt/phospholipid mixed-micelles take up fat-soluble compounds and carry them through the intestinal tract so they can be absorbed in the gut or efficiently secreted in the feces (6). At the terminal ileum, the majority (~95%) of bile salts is reabsorbed and return to the ...
... intestinal lumen. The bile salt/phospholipid mixed-micelles take up fat-soluble compounds and carry them through the intestinal tract so they can be absorbed in the gut or efficiently secreted in the feces (6). At the terminal ileum, the majority (~95%) of bile salts is reabsorbed and return to the ...
Auxin transport routes in plant development
... maxima or gradients between cells. Besides local biosynthesis and the release of active forms from inactive precursors, the major determinant of differential auxin distribution is its directional transport between cells. This regulated polar auxin transport (PAT) within plant tissues appears to be u ...
... maxima or gradients between cells. Besides local biosynthesis and the release of active forms from inactive precursors, the major determinant of differential auxin distribution is its directional transport between cells. This regulated polar auxin transport (PAT) within plant tissues appears to be u ...
Characterization of the Two-Component, FAD-Dependent Monooxygenase SgcC That Requires Carrier Protein-Tethered
... similarities.17–19 The first class, or one-component monooxygenases, are single polypeptides that utilize FAD or FMN as a cofactor and require NADH or NADPH to initiate oxidation of substrates; thus, these monooxygenases have both flavin reductase and monooxygnease activity. The second class, or two ...
... similarities.17–19 The first class, or one-component monooxygenases, are single polypeptides that utilize FAD or FMN as a cofactor and require NADH or NADPH to initiate oxidation of substrates; thus, these monooxygenases have both flavin reductase and monooxygnease activity. The second class, or two ...
CHAPTER 1 INTRODUCTION
... them as a hay crop. It was first cultivated in South Africa in 1903 at Cedara in Natal (Cronje , 1997). It was not until after 1945 that their value as a supplier of feed and food oil was recognised and exploited (Snyder and Kwon , 1987). In South Africa a genetically improved soya bean with increas ...
... them as a hay crop. It was first cultivated in South Africa in 1903 at Cedara in Natal (Cronje , 1997). It was not until after 1945 that their value as a supplier of feed and food oil was recognised and exploited (Snyder and Kwon , 1987). In South Africa a genetically improved soya bean with increas ...
Advances in Chemical Protein Modification
... relied upon reactions at nucleophilic amino acids, particularly cysteine (Cys) or lysine (Lys) residues (Scheme 1).13 Cysteine easily undergoes disulfide exchange to form mixed disulfides14 and also alkylation with suitable electrophiles that include α-halocarbonyls (e.g., iodoacetamide)14 and Michael ...
... relied upon reactions at nucleophilic amino acids, particularly cysteine (Cys) or lysine (Lys) residues (Scheme 1).13 Cysteine easily undergoes disulfide exchange to form mixed disulfides14 and also alkylation with suitable electrophiles that include α-halocarbonyls (e.g., iodoacetamide)14 and Michael ...
Differential recruitment of Dishevelled provides signaling specificity
... Localization of Dsh to the membrane and to filopodia in response to Fz Of the known components of PCP and Wg signaling, Drosophila Dsh is thought to act most immediately downstream of the receptor. We were therefore interested in examining possible interactions between Dsh and the PCP receptor Fz an ...
... Localization of Dsh to the membrane and to filopodia in response to Fz Of the known components of PCP and Wg signaling, Drosophila Dsh is thought to act most immediately downstream of the receptor. We were therefore interested in examining possible interactions between Dsh and the PCP receptor Fz an ...
Analysis of the Arabidopsis rsr4-1/pdx1
... rsr4-1 plants display several prominent aerial and root phenotypes. Flowering time under long-day conditions (16 h light) was delayed, in comparison with wild-type C24 plants (Figure 2C), but mutant plants displayed no change in leaf number at the time of flowering, with an average of 22 rosette lea ...
... rsr4-1 plants display several prominent aerial and root phenotypes. Flowering time under long-day conditions (16 h light) was delayed, in comparison with wild-type C24 plants (Figure 2C), but mutant plants displayed no change in leaf number at the time of flowering, with an average of 22 rosette lea ...
The uptake, metabolism, transport and transfer of nitrogen in an
... Fig. 1 Molecular structure of ions of bis-(trimethylsilyl) trifluoroacetamide (MTBSTFA)-derivatized Arg. This figure gives the structure of the ion remaining after a 70-eV impact on the MTBSTFA derivative of Arg. It demonstrates that the ion being detected has lost one of the quanido Ns and a t-buty ...
... Fig. 1 Molecular structure of ions of bis-(trimethylsilyl) trifluoroacetamide (MTBSTFA)-derivatized Arg. This figure gives the structure of the ion remaining after a 70-eV impact on the MTBSTFA derivative of Arg. It demonstrates that the ion being detected has lost one of the quanido Ns and a t-buty ...
Control of Pre-mRNA Splicing by the General Splicing Factors
... mutations that affect the splicing process are common, and testify to the importance of splicing for normal cellular function. The splicing process is made more complex by the fact that many premRNAs can be spliced in more than one way to give mature transcripts coding for proteins with distinct fun ...
... mutations that affect the splicing process are common, and testify to the importance of splicing for normal cellular function. The splicing process is made more complex by the fact that many premRNAs can be spliced in more than one way to give mature transcripts coding for proteins with distinct fun ...
Responses of plant vascular systems to auxin transport
... developmental stage of inhibitor application. Finally, we will ...
... developmental stage of inhibitor application. Finally, we will ...
Trichomonas vaginalis - Università degli Studi di Sassari
... pancake shape that allows an increasing of the surface contact [2, 14]. In a process called swarming, trophozoites are able to attach one to another by their pseudopods forming aggregates consisting of numerous cells. The exact role of these aggregates is not yet clear. Pereira-Naves et al have show ...
... pancake shape that allows an increasing of the surface contact [2, 14]. In a process called swarming, trophozoites are able to attach one to another by their pseudopods forming aggregates consisting of numerous cells. The exact role of these aggregates is not yet clear. Pereira-Naves et al have show ...
Structure-based design and functional studies of novel noroviral 3C
... substrate peptides and peptide-like inhibitors [13,26–29]. The norovirus NS6 protease is a cysteine protease that adopts a chymotrypsin-like fold consisting of two well-defined domains, a β-sheet domain 1 and a β-barrel domain 2, joined by the 20residue lpeI loop. The two domains are separated by th ...
... substrate peptides and peptide-like inhibitors [13,26–29]. The norovirus NS6 protease is a cysteine protease that adopts a chymotrypsin-like fold consisting of two well-defined domains, a β-sheet domain 1 and a β-barrel domain 2, joined by the 20residue lpeI loop. The two domains are separated by th ...
Escherichia coli FtsZ polymers contain mostly GTP and
... different from that of tubulin, and that FtsZ itself might have a motor role in cell division. ...
... different from that of tubulin, and that FtsZ itself might have a motor role in cell division. ...
Mitochondria as a Pharmacological Target
... facilitate a better understanding of its mechanism of action and open new perspectives of its application. For example, recent studies on the cardioprotective action of potassium channel openers have revealed that cardiac mitochondria are more important as the primary targets of these drugs than is ...
... facilitate a better understanding of its mechanism of action and open new perspectives of its application. For example, recent studies on the cardioprotective action of potassium channel openers have revealed that cardiac mitochondria are more important as the primary targets of these drugs than is ...