Purification and characterization of pyruvate decarboxylase from
... magnetic stirrer. The pH of the medium was adjusted to pH 3.0 with 6.5 M-HCI prior to inoculation and the medium was reduced by the addition of 25 ml titanium/nitrilotriacetic acid solution (Moench & Zeikus, 1983). The cells were harvested during mid-exponential phase by centrifugation using a Sharp ...
... magnetic stirrer. The pH of the medium was adjusted to pH 3.0 with 6.5 M-HCI prior to inoculation and the medium was reduced by the addition of 25 ml titanium/nitrilotriacetic acid solution (Moench & Zeikus, 1983). The cells were harvested during mid-exponential phase by centrifugation using a Sharp ...
Data mining for important amino acid residues in multiple sequence
... products which induce more than one transition state in this particular reaction. As enzymes are such outstanding bio-catalysts, one of the most important goals in biochemical research is to determine the function of an enzyme (and how exactly such an enzyme catalyzes its chemical reaction). Because ...
... products which induce more than one transition state in this particular reaction. As enzymes are such outstanding bio-catalysts, one of the most important goals in biochemical research is to determine the function of an enzyme (and how exactly such an enzyme catalyzes its chemical reaction). Because ...
A low-molecular-mass protein from Methylococcus
... that the FDH may be associated with bound formaldehyde following enzyme isolation and that NAD may also be associated with the enzyme but in a form that is less tightly bound than found with the methanol dehydrogenase from Bacillus methanolicus. Data are presented which indicate that the modifin may ...
... that the FDH may be associated with bound formaldehyde following enzyme isolation and that NAD may also be associated with the enzyme but in a form that is less tightly bound than found with the methanol dehydrogenase from Bacillus methanolicus. Data are presented which indicate that the modifin may ...
Fast evolutionary rates associated with functional loss in class I
... A third glucose transporter gene, sgtp2, has also been identified. The sgtp2 open reading frame was interrupted by a missing base ~260 bp downstream the first initiator ATG codon. The hypothetical addition of a base at this downstream position resulted in a translation product homologous to glucose ...
... A third glucose transporter gene, sgtp2, has also been identified. The sgtp2 open reading frame was interrupted by a missing base ~260 bp downstream the first initiator ATG codon. The hypothetical addition of a base at this downstream position resulted in a translation product homologous to glucose ...
please refer to anzfa`s guide to applications and proposals for a
... required to be labelled as genetically modified if novel DNA and/or novel protein is present in the final food. Studies conducted by the Applicant show that novel proteins are present in the grain. Soybean DP-356043-5 has elevated levels of two minor fatty acids, heptadecanoic acid (C17:0) and hepta ...
... required to be labelled as genetically modified if novel DNA and/or novel protein is present in the final food. Studies conducted by the Applicant show that novel proteins are present in the grain. Soybean DP-356043-5 has elevated levels of two minor fatty acids, heptadecanoic acid (C17:0) and hepta ...
Unusual ADP-forming acetyl-coenzyme A synthetases from the
... Abstract ADP-forming acetyl-CoA synthetase (ACD), the novel enzyme of acetate formation and energy conservation in archaea (Acetyl - CoA þ ADP þ Pi acetate þ ATP þ CoA), has been studied only in few hyperthermophilic euryarchaea. Here, we report the characterization of two ACDs with unique molecul ...
... Abstract ADP-forming acetyl-CoA synthetase (ACD), the novel enzyme of acetate formation and energy conservation in archaea (Acetyl - CoA þ ADP þ Pi acetate þ ATP þ CoA), has been studied only in few hyperthermophilic euryarchaea. Here, we report the characterization of two ACDs with unique molecul ...
Toxoplasma gondii Chitinase Induces Macrophage Activation
... (Km; 0.34 mM) indicated that this chitinase has higher affinity for the substrate than chitinases previously identified in T. cruzi (1.5 mM), Clostridium paraputrificum (7.9 mM), and Trichoderma harzianum (8.06 mM) (Table 1). Although few other chitinases from microorganisms have been described (Tab ...
... (Km; 0.34 mM) indicated that this chitinase has higher affinity for the substrate than chitinases previously identified in T. cruzi (1.5 mM), Clostridium paraputrificum (7.9 mM), and Trichoderma harzianum (8.06 mM) (Table 1). Although few other chitinases from microorganisms have been described (Tab ...
Understanding the Regulation of Metabolic Enzyme Acetylation in E
... metabolic outcomes. Here, we report that the cAMP receptor protein (CRP) regulates protein acetylation in E. coli and provide evidence that protein acetylation modulates the flux of carbon through central metabolism. When we grew cells in mixed amino acids supplemented with glucose and cAMP, global ...
... metabolic outcomes. Here, we report that the cAMP receptor protein (CRP) regulates protein acetylation in E. coli and provide evidence that protein acetylation modulates the flux of carbon through central metabolism. When we grew cells in mixed amino acids supplemented with glucose and cAMP, global ...
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose
... 2-mercaptoethanol did not remove the presence of high molecular mass aggregated Rubisco bands. In contrast, aggregated Rubisco bands formed during oxidization stress disappeared in reductive SDS-PAGE conditions (Peñarrubia and Moreno 1990, Mehta et al. 1992). Attachment of [14C]AsA to Rubisco protei ...
... 2-mercaptoethanol did not remove the presence of high molecular mass aggregated Rubisco bands. In contrast, aggregated Rubisco bands formed during oxidization stress disappeared in reductive SDS-PAGE conditions (Peñarrubia and Moreno 1990, Mehta et al. 1992). Attachment of [14C]AsA to Rubisco protei ...
Engineering the pentose phosphate pathway of
... it is unable to utilise the 5-carbon sugars D-xylose and L-arabinose present in plant biomass. In this study, one key metabolic step of the catabolic D-xylose pathway in recombinant D-xylose-utilising S. cerevisiae strains was studied. This step, carried out by xylulokinase (XK), was shown to be rat ...
... it is unable to utilise the 5-carbon sugars D-xylose and L-arabinose present in plant biomass. In this study, one key metabolic step of the catabolic D-xylose pathway in recombinant D-xylose-utilising S. cerevisiae strains was studied. This step, carried out by xylulokinase (XK), was shown to be rat ...
Diacylglycerol kinase zeta in hypothalamus interacts with long form leptin receptor. Relation to dietary fat and body weight regulation
... by the fact that DGK is widely and abundantly expressed throughout the body (14) and that it can promiscuously use various kinds of long chain diacylglycerols as substrates, whether or not they are second messengers (11). Dietary fat is an important factor that contributes to the development of obe ...
... by the fact that DGK is widely and abundantly expressed throughout the body (14) and that it can promiscuously use various kinds of long chain diacylglycerols as substrates, whether or not they are second messengers (11). Dietary fat is an important factor that contributes to the development of obe ...
as Hotspot Ca 2+ Signaling Units
... resting conditions [32]. They have yet to be identified, although recently strong evidence has been provided that the Na+/Ca2+ exchanger isoform NCLX is the longsought protein responsible for the mitochondrial Na+-dependent Ca2+ efflux [33]. Finally, the low conductance mode of the permeability tran ...
... resting conditions [32]. They have yet to be identified, although recently strong evidence has been provided that the Na+/Ca2+ exchanger isoform NCLX is the longsought protein responsible for the mitochondrial Na+-dependent Ca2+ efflux [33]. Finally, the low conductance mode of the permeability tran ...
Ribosomal Stalk Protein L12: Structure, Function and
... L12 is one of the best studied r-protein. In general, L12 proteins form a strong dimer in solution. The L12 dimer consists of two organized domains connected by one flexible hinge region. The N-terminal domain spanning residues 1-37 is responsible for the strong dimer interaction and for binding to ...
... L12 is one of the best studied r-protein. In general, L12 proteins form a strong dimer in solution. The L12 dimer consists of two organized domains connected by one flexible hinge region. The N-terminal domain spanning residues 1-37 is responsible for the strong dimer interaction and for binding to ...
on the importance of cooperativity effects - RSC Publishing
... in avin-dependent enzymes.26 By examining high-resolution structures of proteins and nucleic acids, Chakravarty and coworkers pointed out that “h6”-type anion–p interaction is observed unambiguously and suggested it plays an important role in macromolecular folding and function.27 Howell and cowork ...
... in avin-dependent enzymes.26 By examining high-resolution structures of proteins and nucleic acids, Chakravarty and coworkers pointed out that “h6”-type anion–p interaction is observed unambiguously and suggested it plays an important role in macromolecular folding and function.27 Howell and cowork ...
2004__MORRIS_et_al_Transpor... - Institute of Experimental Botany
... It is probable that auxin efflux carriers are multi-component systems consisting of at least two, but possibly more individual protein components, each with a different function (61). The synthetic auxin transport inhibitor NPA strongly inhibits auxin efflux and consequently stimulates auxin accumul ...
... It is probable that auxin efflux carriers are multi-component systems consisting of at least two, but possibly more individual protein components, each with a different function (61). The synthetic auxin transport inhibitor NPA strongly inhibits auxin efflux and consequently stimulates auxin accumul ...
Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for
... Lis1 is required for nuclear migration in fungi, cell cycle progression in mammals, and the formation of a folded cerebral cortex in humans. Lis1 binds dynactin and the dynein motor complex, but the role of Lis1 in many dynein/dynactindependent processes is not clearly understood. Here we generate a ...
... Lis1 is required for nuclear migration in fungi, cell cycle progression in mammals, and the formation of a folded cerebral cortex in humans. Lis1 binds dynactin and the dynein motor complex, but the role of Lis1 in many dynein/dynactindependent processes is not clearly understood. Here we generate a ...
Endoplasmic reticulum localization of the low density lipoprotein
... membrane. Epifluorescence microscopy was used to visualize endogenous LDLR, transfected LDLR, and transport-defective LDLR mutants in Ldlr⫺/⫺ (C–F) and wild-type hepatocytes (all other panels). (A and B) Endogenous LDLR was visualized in nonpermeabilized and permeabilized hepatocytes, respectively, ...
... membrane. Epifluorescence microscopy was used to visualize endogenous LDLR, transfected LDLR, and transport-defective LDLR mutants in Ldlr⫺/⫺ (C–F) and wild-type hepatocytes (all other panels). (A and B) Endogenous LDLR was visualized in nonpermeabilized and permeabilized hepatocytes, respectively, ...
Flux analysis in central carbon metabolism in plants
... powerful method of flux quantification, that is gaining popularity in metabolic engineering. Flux quantification in plants has received very limited attention compared to that in microorganisms and mammalian cells. This is principally because plant biochemistry is more complex, and exhibits extensiv ...
... powerful method of flux quantification, that is gaining popularity in metabolic engineering. Flux quantification in plants has received very limited attention compared to that in microorganisms and mammalian cells. This is principally because plant biochemistry is more complex, and exhibits extensiv ...
The potential for toxin and antitoxin gene
... may be more successful on horizontally mobile elements than others. This includes genes that exhibit a post-segregational killing (PSK) phenotype. PSK is generated by expression of a toxin and antitoxin from a mobile element, such that if a bacterium loses the element the toxin becomes active in the ...
... may be more successful on horizontally mobile elements than others. This includes genes that exhibit a post-segregational killing (PSK) phenotype. PSK is generated by expression of a toxin and antitoxin from a mobile element, such that if a bacterium loses the element the toxin becomes active in the ...
New insight into the photoheterotrophic growth of the
... also employs the EMC pathway. On the other hand, Rs. rubrum has been proposed to assimilate acetate through the citramalate cycle (Ivanovsky et al., 1997), based on the early finding that acetate assimilation is accompanied by the production of a compound identified as citramalate (Hoare, 1963) and ...
... also employs the EMC pathway. On the other hand, Rs. rubrum has been proposed to assimilate acetate through the citramalate cycle (Ivanovsky et al., 1997), based on the early finding that acetate assimilation is accompanied by the production of a compound identified as citramalate (Hoare, 1963) and ...
Cbp3–Cbp6 interacts with the yeast mitochondrial ribosomal tunnel
... mRNAs, we used strains carrying intronless mitochondrial genomes. Thus, the mRNAs are present in constant quantities, allowing us to assess their translation independent of the splicing processes. Mitochondrial translation products of wild type or cells lacking CBP3 were pulse labeled with [35S]meth ...
... mRNAs, we used strains carrying intronless mitochondrial genomes. Thus, the mRNAs are present in constant quantities, allowing us to assess their translation independent of the splicing processes. Mitochondrial translation products of wild type or cells lacking CBP3 were pulse labeled with [35S]meth ...