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Microbial Metabolism PowerPoint
Microbial Metabolism PowerPoint

... c) redox energy is used to pump H+ into the cell i) creates a higher concentration in ICF d) H+ is moved out through ATPsynthase creating ATP as it moves out e) each NADH has enough energy to produce 3 ATP and each FADH2 can produce 2 i) 30 ATP from NADH and 4 from FADH2 ...
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... NADH + H+ + FMN  NAD+ + FMNH2 FMNH2 + (Fe-S)ox  FMNH· + (Fe-S)red + H+ After Fe-S is reoxidized by transfer of the electron to the next iron-sulfur center in the pathway: FMNH· + (Fe-S)ox  FMN + (Fe-S)red + H+ Electrons pass through a series of iron-sulfur centers, and are eventually transferred ...
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... the mitochondrial matrix and the intermembrane space, which, requires ejecting of hydrogen ions into the inter-membrane space in the course of electron transportation for synthesis of water. The structures responsible for transporting ions must do so in a predetermined direction and maintain functio ...
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... Preservative: Thiomersal 0.005%. Pharmacological Action Cytochrome C is involved in the oxidative phosphorylation for synthesizing ATP from ADP. Anhydrous Sodium Succinate (intermediate substance) promotes the production of ATP. Adenosine plays essential role in producing energy required for the vit ...
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Oxidative phosphorylation



Oxidative phosphorylation (or OXPHOS in short) is the metabolic pathway in which the mitochondria in cells use their structure, enzymes, and energy released by the oxidation of nutrients to reform ATP. Although the many forms of life on earth use a range of different nutrients, ATP is the molecule that supplies energy to metabolism. Almost all aerobic organisms carry out oxidative phosphorylation. This pathway is probably so pervasive because it is a highly efficient way of releasing energy, compared to alternative fermentation processes such as anaerobic glycolysis.During oxidative phosphorylation, electrons are transferred from electron donors to electron acceptors such as oxygen, in redox reactions. These redox reactions release energy, which is used to form ATP. In eukaryotes, these redox reactions are carried out by a series of protein complexes within the inner membrane of the cell's mitochondria, whereas, in prokaryotes, these proteins are located in the cells' intermembrane space. These linked sets of proteins are called electron transport chains. In eukaryotes, five main protein complexes are involved, whereas in prokaryotes many different enzymes are present, using a variety of electron donors and acceptors.The energy released by electrons flowing through this electron transport chain is used to transport protons across the inner mitochondrial membrane, in a process called electron transport. This generates potential energy in the form of a pH gradient and an electrical potential across this membrane. This store of energy is tapped by allowing protons to flow back across the membrane and down this gradient, through a large enzyme called ATP synthase; this process is known as chemiosmosis. This enzyme uses this energy to generate ATP from adenosine diphosphate (ADP), in a phosphorylation reaction. This reaction is driven by the proton flow, which forces the rotation of a part of the enzyme; the ATP synthase is a rotary mechanical motor.Although oxidative phosphorylation is a vital part of metabolism, it produces reactive oxygen species such as superoxide and hydrogen peroxide, which lead to propagation of free radicals, damaging cells and contributing to disease and, possibly, aging (senescence). The enzymes carrying out this metabolic pathway are also the target of many drugs and poisons that inhibit their activities.
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