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Quiz #3
BE 109 SP 03 MON/WED
4/9/03
Name_____________________
Write your name on this quiz and then answer the following questions in three sentences
or LESS. You have fifteen minutes to answer ten questions.
1. When you input the mRNA sequence of your gene of interest into Ambion’s website,
what nucleotide pattern does it look for to come up with a list of candidate siRNA
sequences?
2. Why is it important to maintain DNA replication fidelity? Is it acceptable to have a
one in a million error rate?
3. What are some of the causes of DNA damage?
4. How does having defects in DNA repair pathways affect human health?
5. What general category of DNA repair are EXO1 and AAG involved in?
6. What is the specific role of exonuclease-1 in this type of DNA repair? That is, which
step does it accomplish?
7. How do E. coli distinguish between parental and newly replicated strands when
performing DNA mismatch repair? For instance, if a T was wrongly paired with a G, how
does the cell know which base to replace?
8. ATR, ATM, and p53 are all involved in pathways that allow the cell to do what in
response to DNA damage signals?
9. Why has it been difficult to produce an ATR knockout mouse? Hint: what is the
phenotype of ATR knockout cells?