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Microbiology Review Guide Answers Viruses 1. A virus is a small pathogen which causes many diseases. 2. Viruses are non-living because they don’t carry out life processes such as metabolism, growth, & development. All are parasitic – require hosts. 3. True: Viruses are specific as to what type of host or tissue they infect. 4. Ebola, AIDS, colds, influenza, chicken pox, polio, etc. are all viral diseases. 5. Interferon is a protein produced by the body that interferes with viral replication. 6. Ebola kills its victims too quickly so it is unable to spread very far. 7. Viruses consist of a nucleic acid (DNA or RNA) wrapped in a capsid (protein coat). 8. Viruses are classified by the type of nucleic acid they consist of, shape, mode of transmission, etc. 9. People don’t get sick every time they encounter a virus because they may be immune (have antibodies), they may have destroyed it with other defenses, such as stomach acid, it may be a virus that does not infect humans . . . Bacteria 1. Bacteria belong to the Archaebacteria or Eubacteria Kingdoms. 2. Bacteria are classified by shape, arrangement, gram stain reaction, oxygen requirements, colony color/appearance, etc. 3. See diagram on Bacteria WS & Quiz 4. An endospore is an adaptation for protection, it is a structure that allows the bacterial cell to remain dormant when environmental conditions are unfavorable & when conditions improve, the cell will resume normal functioning. 5. Obligate aerobes require oxygen for energy, obligate anaerobes do not use oxygen and may be harmed by it, facultative anaerobes can use oxygen when available but can also use fermentation for energy when oxygen is not available. 6. Most bacteria eat carbon based compounds such as fat, protein, sugar, or blood. 7. Parasitism is a relationship in which one organism benefits and the other is harmed; Mutualism is a relationship in which both organisms benefit. 8. Bacteria are able to convert nitrogen in the air into soil compounds that plants can use; they are used to make many food products, including yogurt, cheese, pickles, etc., they aid in digestion, are used for vaccines, wrinkle treatment, leather tanning, producing beer & wine, genetic engineering of human hormones etc. Bacteria can also be harmful because they cause food spoilage & disease. 9. Most bacteria prefer warm, dark, moist environments with the correct oxygen requirements & a food source. 10. Binary fission is a form of asexual reproduction – identical bacteria are produced; Conjugation is a form of sexual reproduction in which bacteria can share their genetic material & therefore pass on mutations. Labs 1. Agar in a Petri dish provides a food source for the bacteria. 2. Bacteria are mixed with water when making a smear to dilute them so that individual cells can be seen. 3. Heat fixing bacteria helps them to stick to the slide & not wash off during the addition of chemicals & rinsing. 4. True: the gram reaction is based on cell wall chemistry. 5. A gram + streptococcus would be a purple chain of spheres. 6. True: A colony consists of bacteria that are genetically alike. 7. ------- 8. ------- 9. ------Spread of Disease 1. Direct Contact 2. Botulism 3. Food 4. Salmonella 5. Airborne droplets 6. Anything that can carry & transmit disease; Lyme disease – deer tick; AIDS – dirty needle 7. Boil water, filter water, chemically treat water, cook food thoroughly, wash hands, refrigerate or freeze perishables . . . 8. Many different strains exist & they mutate rapidly. 9. Disinfect objects/surfaces; don’t share personal hygiene items; wear shower shoes, etc. Defenses Against Disease 1. Helper T cells – identify invaders & alert the immune system; killer T cells destroy infected body cells by using powerful chemicals; memory cells – store information for preparing antibodies; B cells – produce antibodies 2. Fever speeds up the rate of tissue repair & slows down pathogen growth 3. Stomach acid kills many swallowed pathogens 4. A vaccination is an injection of dead or inactivated pathogens that cause the body to produce antibodies to that particular pathogen = immunity 5. Mucus, skin, saliva, interferon, complement, phagocytes, etc. are all physical or chemical defenses against pathogens 6. Histamine is the substance produced by white blood cells that causes inflammation 7. Interferon is a non-specific chemical defense that “kills” viruses 8. Passive Immunity Active Immunity Where antibodies made Someone else Your own body How acquired naturally Placenta/Breast milk Exposure to pathogen How acquired artificially Injection of antibodies Vaccination How long it takes to develop Works immediately ~1 month or so How long immunity lasts Short time – few Long time – months, weeks years or permanent Are memory cells involved? No Yes Parasites 1. Tapeworm 2. Schistosoma (Swimmer’s Itch) 3. Trichina 4. Hookworm Protists 1. False – All protists are not multicellular; most are unicellular 2. True 3. True 4. False – Sporozoans are animal-like protists that cannot move 5. Cilia = small hairs in large numbers & flagella = long, whip-like structures only 1 or 2 total. 6. Ameoba 7. Malaria, African Sleeping Sickness, Chagas Disease – bite of infected arthropod 8. True 9. Euglena has no cell wall and can move = animal-like BUT also has chlorophyll to make its own food = plant-like 10. They supply ~75% of the world’s oxygen