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Name _______________________________ Date ___________ Per _____ Protein Synthesis Overview Label the summary of protein synthesis diagrammed below using the following terms: transcription, translation, DNA, mRNA, ribosome, tRNA, amino acid, polypeptide, nucleus, codon, and anticodon. What is it? DNA mRNA tRNA Amino acids Transcription translation Where is it? Picture TRANSCRIPTION For the following examples, give the appropriate sequence of DNA, mRNA, tRNA and/or polypeptide (AA = amino acids). Remember: A codon chart can only be used for decoding a strand of mRNA. Example 1: DNA: TAC GCG CCT AGG GGG TGG mRNA: _____ _____ _____ _____ _____ _____ AA: _____ _____ _____ _____ ______ ______ Example 2: DNA: TAC GCG CCT AGG GGG TGG mRNA: _____ _____ _____ _____ _____ _____ tRNA: _____ _____ _____ _____ ______ ______ AA: _____ _____ _____ _____ ______ ______ Example 3: DNA: C__ _G_ A__ _A_ __C _T_ mRNA: _U_ __A __C __A _G_ _A_ tRNA: _AU G_U _UG G_U CC_ G_A AA: _____ _____ _____ _____ _____ _____ DNA to Protein Worksheet- HARDER!!! My name is: __________________________ Write the corresponding strand of DNA for each codon below. CAT TAG GCC AGC TGA CTT GAC CAG GAG TGC ________________________________________________________ Write the corresponding strand of mRNA for each DNA codon below. CAT TAG GCC AGC TGA CTT GAC CAG GAG TGC ________________________________________________________ Write the corresponding tRNA anti-codons for each mRNA codon below. CAU UAG GCC AGC UGA CUU GAC CAG GAG UGC ______________________________________________________ Write the amino acids for each mRNA codon below. CUA ___________ AUC ____________ CGG ________________ UCG ____________ What anit-codon on tRNA would bind with these mRNAcodons? a. AAA b. GGA c. UAC d. CGU What amino acids would the translation of these mRNA transcripts produce? a. UAA CAA GGA GCA UCC b. UGA CCC GAU UUC AGC 1. 1. 2. 2. 3. 3. 4. 4. 5. 5. DNA to Protein Worksheet- Easier EXPLAIN these terms: DNA Replication: Transcription: Translation: My name is: _________________________ Codon: Anti-codon: Amino Acids: 1. Write the corresponding DNA strands for the DNA strands below: ATT CAT TAG GAG CAC TAC GCA __________________________ CGA TCG GAT CCA CAG TGA CGA __________________________ TGA CGA CCG GGG TCC GAT CCA __________________________ 2. Write the corresponding mRNA strands for the DNA strands below: ATT CAT TAG GAG CAC TAC GCA __________________________ CGA TCG GAT CCA CAG TGA CGA __________________________ TGA CGA CCG GGG TCC GAT CCA __________________________ 3. Write the corresponding tRNA anti-codons for each mRNA codon below: AUU CAU UAG GAG CAC UAC GCA __________________________ CGA UCG GAU CCA CAG UGA CGA __________________________ UGA CGA CCG GGG UCC GAU CCA __________________________ Name _______________________________________ Date ___________ Per ____ DNA: The Molecule of Heredity 1. A nucleotide is made of three parts: a ___________________ group, a five carbon __________________, and a _________________ base. 2. In a single strand of DNA, the phosphate group binds to the __________________ of the next group. 3. Chargaff's rule states that the DNA of any species contains equal amounts of ______________ & __________________ and also equal amounts of _________________ & _________________. 4. In DNA, thymine is complementary to (or pairs with) ________________ ; cytosine is complementary to _____________. 5. In a strand of DNA, the percentage of thymine is 30%. What is the percentage of cytosine in the same DNA strand? _________________ 6. James Watson and Francis Crick with, the help of Rosalind Franklin and others, determined that the shape of the DNA molecule was a __________________________. 7. On the diagram to the right: Circle and label a nucleotide. Label the sugar and phosphate molecules. Label the bases that are not already labeled. Label a base pair. Label the sugar-phosphate backbones. Label the hydrogen bonds. DNA Replication 1. Number the steps of DNA replication in the correct order (1, 2, 3) _______Daughter strands are formed using complementary base pairing. ______ DNA unwinds ______ The DNA of the daughter strands winds with together with its parent strand. 2. Why is DNA replication called "semi-conservative"? ________________________________ 3. What enzyme unwinds or unzips the parent strand? _________________________________ 4. What enzyme connects the new bases to the old bases in the DNA template? ___________________________________________________________________ 5. What enzyme connects the new nucleotides together and proofreads them? ___________________________________________________________________ 6. Show the complimentary base pairing that would occur in the replication of the short DNA molecule below. Use a colored pencil (or different pens, markers, etc.) to show which strands are the original and which are newly synthesized. Original DNA Strand 1 ---Original Original DNA ---DNA Strand 1 (copy ---Strand 2 from left) > A-T C-G T-A T-A A-T C-G G-C C-G C-G G-C A-T T-A New New DNA + DNA Strand Strand Original DNA Strand 2 (copy from left)