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STATE UNIVERSITY COLLEGE AT BUFFALO Department of Biology Biology 100 Principles of Biology Fall 2004 Dr. Wadsworth Mid-Term Exam 1a Biomolecules, Membranes and Cells Cover Sheet Name: ____________________________________________________ (Print) Instructions. 1. Print your name in the space designated on this cover sheet. 2. Be sure that your exam has 8 pages including this cover sheet. 3. Read each question carefully and answer in the space provide 4. At the end of the exam there are 6 short answer questions. Answer only 5 of these 6 questions. Answering all six questions may reduce your grade. Under the question you choose not to answer, please write the word "Skip". Failure to write "SKIP" under the one question you choose not to answer will reduce your grade. Multiple Choice Choose the best answer for the question or the best ending for each statement. Write the letter (A-E) which corresponds to the best answer on the line before the question. (2 pts each) _____ 1. Which of the following might describe the subatomic composition of a positively charged nitrogen ion? A. B. C. D. E. 7 protons, 6 neutrons, 7 electrons 7 protons, 7 neutrons, 6 electrons 8 protons, 7 neutrons, 7 electrons 8 protons, 6 neutrons, 7 electrons 8 protons, 7 neutrons, 6 electrons _____2. What are the four most abundant elements found in biological molecules? A. B. C. D. E. Hydrogen, Sulfur, Nitrogen, and Oxygen Carbon, Oxygen, Hydrogen, and Nitrogen Phosphorus, Hydrogen, Carbon, and Oxygen Carbon, Oxygen, Nitrogen, and Sulfur Carbon, Hydrogen, Sulfur and Phosphorus _____3. Which of the following would convert a neutrally charged hydrogen atom into a hydrogen ion? A. B. C. D. E. Addition of a charged proton. Addition of two positively charged protons Loss of an electon Any change in the subatomic structure Loss of a proton _____ 4. How does an atom with a valence number of 1 differ from an atom with a valence number of 2? A. The atom with the valence number of 2 has twice the electrons as the atom with the valence number of 1. B. The atom with the valence number of 2 can form twice as many covalent bonds as the atom with the valence number of 1. C. The atom with the valence number of 2 is more electronegative than the atom with the valence number of 1. D. The atom with the valence number of 2 is twice as large as the atom with the valence number of 1. E. The atom with the valence number of 2 has one more electron than the atom with a valence number of 1. _____ 5. The protein that causes mad cow disease is a prion. Which of the following best explains why it is a prion? A. B. C. D. E. It forms plaques in the brain that result in a loss of memory. It assembles into long rods in red blood cells causing them to take a sickle shape. It produces fever and fatigue. It induces the misfolding of proteins in individuals that ingest it. It leads to the substitution of hydrophobic amino acids for hydrophilic amino acids. _____ 6. Polymers are broken down to form monomers by an hydrolysis reaction. Which of the following best describes why this reaction is called a hydrolysis reaction? A. A water molecule is split to restore the hydroxyl and hydrogen on the monomers B. The energy stored in the molecule is released during splitting and temporarily stored in water. C. A water molecule needs to be divided into an hydroxyl and a hydrogen to release energy to split the polymers. D. A hydroxyl and hydrogen are split from the polymer to form a new water molecule. E. The chemical bonds within a polymer are much like those found in water. _____7. When a protein is in its native state, where would you expect to find the amino acids with hydrophilic side groups? A. Covalently linked to one another to form the peptide bonds B. Covalently linked to hydrophobic amino acids to form peptide bonds C. Buried in the interior of the protein, bound to water D. Linked to one another through hydrophilic interactions E. Near the surface of the protein _____ 8. Nucleotides are made of which of the following sets of components? A. Nitrogenous base, Carboxyl Group, Sugar B. Amino acid, Fatty Acid, Sugar C. Hydroxyl, Phosphate, Carboxyl D. Amino acid, Nitrogenous base, Sugar E. Sugar, Phosphate, Nitrogenous base _______ 9. Which of the following classes of molecules consist of four interlocking carbon rings modified with various functional groups? A. Steroids B. Fatty Acids C. Triglyceride D. Polypeptides E. Cellulose _____ 10. Glucose is an example of which of the following classes of biomolecules? A. Proteins B. Amino Acids C. Nucleotides D. Monosaccharides E. Triglycerides _____ 11. Which of the following classes of biomolecules can serve as a hormone in humans? A. B. C. D. E. RNA and DNA Proteins and Nucleic Acids Proteins and Steroids Proteins and Polysaccharides Polysaccharides and Steroids _____ 12. The phrase "Primacy of Proteins" is used to emphasize: A. B. C. D. E. that proteins are the largest of the biomolecules that proteins contain "information" that proteins are hydrophobic that proteins are highly hydrophilic that proteins perform most cellular functions _____ 13. Which of the following molecules would have the most atoms. A. B. C. D. E. Saturated triglyceride Saturated fatty acid Unsaturated fatty acid Polyunsaturated fatty acid Polyunsaturated triglyceride _____ 14. Which of the following are functions of carbohydrates in cells? A. B. C. D. E. Storage of energy and hormones Motion and membranes Membranes and cell walls Storage of energy and structural Hormones and structural _____ 15. Which of the following parts of the cell is larger? A. B. C. D. E. Cytoplasm Ribosome Nucleus Mitochondria Chromatin _____ 16. Which of the following organelles would you find in plant cells but not animal cells? A. Nucleus B. Cytoskeleton C. Central Vacuole D. Mitochondria E. Vesicles _____ 17. What organelle is most responsible for assembling membranes? A. Endoplasmic Reticulum B. Plasma Membrane C. Choloroplasts D. Mitochondria E. Cytoskeleton _____ 18. Which of the following is found in both eukaryotic and prokaryotic cells? A. Endoplasmic Reticulum B. Nucleus C. Mitochondria D. Ribosomes E. Golgi _____ 19. Which of the following does not increase the fluidity of membranes? A. B. C. D. E. Increasing temperature Double bonds in the fatty acid chains Cholesterol Unsaturated fatty acids Saturated fatty acids _____ 20. Which of the following best describes a difference between facilitated transport and active transport. A. Active transport requires energy facilitate diffusion doesn’t B. Active transport moves molecules from areas of high concentration to areas of low concentration, and facilitated diffusion moves molecules from areas of low concentration to high concentration. C. Active transport uses a membrane protein facilitated diffusion doesn’t. D. Active transport moves macromolecules across the membrane, while facilitate diffusion moves ions across membranes E. Active transport is specific to a limited set of molecules, while facilitated diffusion can transport any small uncharged molecule. _____ 21. Which of the following best describes the arrangement of intergral membrane proteins in a membrane? A. B. C. D. E. The proteins are sandwiched between layers of phospholipid bilayer The proteins coat the outside of the phospholipid bilayer The proteins pierce the phospholipid bilayer The proteins clump on the surface of the phospholipid bilayer The proteins only interact with the hydrophobic tails of the phospholipids _____ 22. The glycine pump that transports the amino acid glycine across the membrane can not transport the amino acid alanine across the membrane. What explain why the glycine pump cannot transport alanine? A. B. C. D. E. Alanine can not be easily hydrolized Alanine is a triglyceride and, therefore, is too hydrophobic to move across the membrane Alanine does not have a shape complementary to the binding site of the glycine pump Alanine is only found outside of cells Alanine is an ion and ions are never transported across the membrane by active transport. _____ 23. During endocytosis: A. materials stored in the cytoplasmic vesicles are transported to the plasma membrane and ejected. B. Solutes containing necessary materials pass directly into the cell through the plasma membrane. C. Carrier proteins ferry large molecules through the membrane. D. Pumps concentrate large molecules inside of cells. E. Materials are enclosed in invaginations of the plasma membrane, which subsequently pinch off, forming cytoplasmic vesicles. _____ 24. Which of the following molecules uses simple diffusion to move across the plasma membrane: A. B. C. D. E. LDL particles Very small ions Small uncharged particles Proteins Large bulky particles _____ 25. Biological membranes are called selectively premeable membranes because: A. They are composed of about 50% protein and 50% lipids B. Charge molecules can not pass through the membrane C. Active transport requires energy D. Active transport requires a carrier protein E. Only some molecules pass through the membrane Short Answer Questions Answer only five of the following six questions in the space below each question. Under the one question you choose not to answer, write the word "SKIP". 26. Milk proteins remain dissolved in solution when in their native state but clump together to form curds in their denatured state. What explains this difference in the way native and denatured proteins behave? 27. Draw the structure of glucose. 28. Describe three differences between plant and animal cells. 29. Plants around the equator often have saturated fats in their membranes, while plants near the arctic often have unsaturated fatty acids in their membranes. What advantage is it to the arctic plants to have unsaturated fatty acids in their membranes? 30. Biological membranes are described as being semipermeable. An example of this is that some monosaccharides such as glucose can cross the membrane, but other monosaccharides such as raffinose cannot. What is the likely explanation for the difference in the permeability of these two equally sized monosaccharides? 31. The structure of the amino acid phenylalanine is drawn below. Explain whether this molecule could be described as amphipathic or not. H O H N C C H H O CH H C H H H C C C CH C H