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Study Guide for Lab Exam I Microscopes: What are the Rules for Care & Use? ____________________________________________________________ ___________________________________________________________________________________ ___________________________________________________________________________________ What TWO features of our microscope will allow us to adjust the lighting used to view a specimen? ___________________________________________________________________________________ What type light is used by a Compound Scope? _________________ Dissecting Scope? ________________ What is the orientation of the image with the Compound Scope vs. the Dissecting Scope? _________________ ___________________________________________________________________________________ How is Total Magnification calculated? _________________________________________________________ Define the term Magnification ________________________________________________________________ Define the term Resolution ___________________________________________________________________ Be able to name the major parts of the Compound Microscope and give their functions. Cells & Mitosis: Be able to recognize the following features on generalized Animal & Plant Cell Models: Cell Membrane Nucleus Mitochondrion Endoplasmic Reticulum Central Water Vacuole Cell Wall Ribosome Chloroplast Cytoplasm Centrosome Be able to distinguish between the different stages of the Cell Cycle (Interphase & Mitosis) Know the following: Definition, Purpose, and Significance of Mitosis Know the following features of mitosis: Chromosomes & Sister Chromatids Equatorial Plane Centromere Spindle Fibers Be able to distinguish between differences in Cytokinesis of Animal Cells and Plant Cells Animals have the formation of a ________________ Plants have the formation of a _________________ Monera & Protista: These will not be on your Exam (Fall ’03) Recognize members of the Kingdom Monera ____________________________________________________ Know examples of Bacterial Diseases. __________________________________________________________ What does a “bacterial colony” on an agar plate represent? ___________________________________________ Recognize members of the Kingdom Monera ____________________________________________________ Know examples of Protozoan Diseases. __________________________________________________________ What is a Vector? Example? __________________________________________________________________ Know locomotion features used by the Protozoans studied in lab: Euglena uses _________________________________________________________________________ Paramecium uses ______________________________________________________________________ Ameba uses __________________________________________________________________________ Physical Properties: Know the terms – 1) Brownian Movement 2) Diffusion 3) Osmosis 4) Dialysis 5) Test for Starch (Iodine) 6) Test for Glucose (Benedict’s) 7) Solution 8) Solvent 9) Solute Characteristics of Active Transport Processes – _____________________________________________________ Characteristics of Passive Transport Processes – ____________________________________________________ Know the term “Selective Permeability” – _________________________________________________________ Know definitions of the terms below AND know the consequence for a cell exposed to this external environment 1) Isotonic – _________________________________________________________________________ Consequence: __________________________________________________________________ 2) Hypotonic – _______________________________________________________________________ Consequence: __________________________________________________________________ 3) Hypertonic – ______________________________________________________________________ Consequence: __________________________________________________________________ Enzymes: What is the role of an Enzyme in a Chemical Reaction? ______________________________________________ How can Enzymes be Denatured? ________________________________ Inhibited? _____________________ Be very familiar with the Characteristics of Enzymes: _______________________________________________ _____________________________________________________________________________________ In our Experiment – What was the Enzyme? ______________________ and its Substrate? __________________ What was the Data for our experiment? ___________________________________________________________ DNA & Protein Synthesis: When during the Cell Cycle does DNA perform Semiconservative Replication? __________________________ Be able to “go back and forth” between DNA codons, mRNA codons, tRNA anticodons, and Amino Acids 1) DNA complementary bases (DNA to DNA) 4) tRNA to mRNA 2) DNA to mRNA 5) mRNA to tRNA 3) mRNA to DNA 6) amino acids called for by DNA , mRNA, tRNA What is Transcription and where does it occur? ____________________________________________________ What molecule is made during Transcription? _____________________________________________________ What is Translation and where does it occur? ______________________________________________________ What molecule is made during Translation? _______________________________________________________ Algae & Lower Plants: Algae are currently classified in which Kingdom? __________________________________________________ Be able to recognize examples of RED, GREEN, & BROWN algae. Know the Anatomy of the Sea Kelp: Blades Stipe Holdfast Air Cyst (bladder) Know some of the Commercial Uses of Algae (Carrageenan, Alginate, Agar, Alginic Acid) 2 Know some of the Commercial Uses of Diatoms ___________________________________________________ Be able to distinguish between the Gametophytes of Mosses & Liverworts. What advancement have Ferns made over Mosses & Liverworts. (Features that have made Ferns “more terrestrial”. _____________________________________________________________________________________ What are the Fern’s Sori? ______________________________________________________________________ Know the Generalized Plant’s Alternation of Generations – Recognize dominant generations, spores, gametes, Sporophyte, Gametophyte, mitosis, meiosis, etc. Gymnosperms & Angiosperms: What is the dominant generation for these two groups of plants? Gametophyte or Sporophyte What advancement have the Gymnosperms & Angiosperms made over the Ferns, Mosses, & Liverworts? _____________________________________________________________________________________ Recognize common examples of Gymnosperms. (Conifers, Cycads, Gingko, Ephedra) Be able to distinguish between Female Cones and Male Cones Recognize examples of different Angiosperms. (Anthophytes) Be able to distinguish between Monocots & Dicots. (cotyledons, floral whorls, roots, leaf venation) Know the floral features of a Perfect & Complete Flower. Know the functions of the floral features too! Draw a Flower here: Sepals _________________________________ Petals _________________________________ Stamen: Anther ______________________________ Filament _____________________________ Pistil: Stigma ______________________________ Style ________________________________ Ovary _______________________________ .Know the story of Pollination and Fertilization for the Angiosperms. _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ What are the two (2) results of the Angiosperm’s Double Fertilization? ___________________________________ ______________________________________ Following Fertilization – What is the fate of the Ovule? ______________________________________________ Following Fertilization – What is the fate of the Ovary? ______________________________________________ 3