Download EEB-20

Laboratory Learning Program
Research Opportunity
Research Opportunity Number: EEB-20
Project Title: Characterizing immunogenetic diversity in canids exposed to sarcoptic mange
Project Summary: It is widely established that infectious disease can pose a serious threat to wildlife. By
increasing morbidity and mortality in populations, the inability to cope with novel and enduring
parasites can precipitate population declines and extinction. Maintaining high levels of genetic diversity
at immune genes (such as the major histocompatibility complex or MHC gene family) is one way to
buffer against this risk. As a result, it is critically important to characterize MHC diversity in vertebrates
exposed to parasites. Sarcoptic mange is one such parasite currently afflicting canids across North
America. Caused by an ectoparasitic mite, sarcoptic mange can result in emaciation, decreased mobility,
and even death if an individual is unable to clear infection. To examine individual differences in
susceptibility and severity, we will genotype MHC microsatellites in wolves and coyotes exposed to this
ectoparasite. This will enable characterization of MHC diversity specific to mange and contribute to the
growing body of research detailing the importance of immunogenetic diversity in mitigating the risk of
wildlife disease.
Student Roles and Responsibilities: Assist in DNA visualization, DNA amplification, and genotyping.
Additional Considerations: Start and end dates are flexible. The student should be able to dedicate at
least 12 hours per week, in time blocks of 4 hours.
Department/Institute: Ecology and Evolutionary Biology
Faculty Sponsor: Bridget vonHoldt
Participation Dates: May to September, 2016
Stipend Offered: $0
Number of Internships Available: 0-1
Application Deadline: March 15, 2016, midnight eastern daylight time