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Mr. Wright, 2010 Section 19.1 Question 1: How are they different? Has no membrane bound nucleus. Has no membrane bound organelles (chloroplasts, mitochondria, etc.) Much simpler cells Has a nucleus. Contains membrane bound organelles Cells are more complex than prokaryotic cells. Prokaryotes Eukaryotes Question 2: Prokaryotes make up two domains: TO CLARIFY – when people talk about everyday bacteria, they are talking about Eubacteria. A bit about Archaea… Archaeabacteria – “ancient bacteria” Have different cell walls than those seen in other living organisms. Found in very extreme environments: Swamps Salt Lakes Deep Sea Vents Hot Springs Archaea and You What is global warming? Some archaea produce methane while making energy and release this in to the environment. Methane = greenhouse gas Cows have a huge impact on global warming. Why? Question 3: 3 Bacterial Shapes: …plus a little extra. The prefix staph- means the cells are grouped into clusters. The prefix strept- means the cells are arranged in chains. Question 4: A Typical Bacterial Cell: What do these things do? Genome Genetic Material (DNA) of the cell Ribosomes Make proteins Cytoplasm Fluid filling the inside of the cell What do these things do? Cell Membrane & Cell Wall Protects and supports the cell. Flagella Used for movement Pili Used to adhere to surfaces and other cells Question #4: Oxygen Requirements Obligate anaerobes – cannot live where there is oxygen Facultative anaerobes – can live with or without oxygen Obligate aerobes – require oxygen to live Question 5: Bacteria can be: Photoautotrophs Get their energy from sunlight Chemoautotrophs Get their energy from inorganic sources Heterotrophs Get their energy from other organisms. MOST BACTERIA Question 6 Binary Fission Binary fission results in two daughter cells that are exactly identical to the mother cell. Question 8: Conjugation Two bacterial cells can join and exchange genetic information using plasmids. “Prokaryotic sex” Plasmid – smaller circular molecules of DNA in the cytoplasm Other methods: Bacterial cells can take up DNA from the environment (Transformation) Other Methods: Viruses can transfer new genetic material into a bacterial cell. (Transduction) Question #9: Endospores Some bacteria can form an endospore when environmental conditions become harsh. Can resist extreme conditions. When good conditions return, the spores give rise to normal bacterial cells. Question #10: Nitrogen fixing bacteria Almost 80% of Earth’s atmosphere is made up of nitrogen gas, but plants and animals cannot utilize it in this form. Nitrogen fixing bacteria convert this nitrogen gas into a form that plants and animals can use – nitrogen fixation Bacteria in Industry Bacteria provide many benefits to humans, as well. We use bacteria to produce many different types of foods. Yogurt Sour Cream Cheeses Bacteria in Industry Bacteria are also used to make certain chemicals. Acetone Antibiotics Insulin We use bacteria to help recycle and break down organic wastes. Oil spills Sewage Bacteria as art! Section 19.3 Bacteria and Humans Some bacteria cause disease in humans by making toxins or by destroying tissue. Examples of bacterial diseases: Anthrax Lyme Disease Staph Infection Salmonella food poisoning Germ Theory Germ Theory was the idea that illness was caused by bacteria. Proven by Louis Pasteur. Invented the first vaccine. Made milk safer to drink through Pasteurization. Antibiotics Antibiotics kill bacteria by interfering with cellular activities. Made naturally by fungi and other bacteria to kill neighboring cells competing for resources. Penicillin comes from a fungus. Antibiotics – The Discovery The first antibiotic (Penicillin) was discovered by Alexander Fleming. It was an accident. Fleming was working with bacteria in the lab. One day, as he was heading home for the weekend, he got lazy and left his dishes out to clean on Monday. When he came back the next day, he discovered Penicillium growing on his plates… with no bacteria remaining. Antibiotic resistance Bacteria are evolving to become resistant to antibiotics. A mutation giving resistance to the antibiotic. While the old strain is wiped out by antibiotics, the new strand continues to multiply and take over. Reasons it’s a big problem: Doctors overprescribe antibiotics when they might not be completely necessary. People “stockpile” extra antibiotics and use them whenever they are sick. Reasons it’s a big problem: Bacteria that we used to have no problem treating are becoming untreatable… and it’s only getting worse!