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“La Caixa” – INPHINIT PROGRAMME – Call for projects Project Title / Job position title: Structural/functional study of a transcripton initiation complex Área of Knowledge (choose one): X Life Sciences Panel Physical Sciences, Mathematics and Engineering Panel Group of disciplines (choose one): X Medicine, Public Health, Sports Science, Nutrition, Clinical Psychology, Healthcare Management Plant, Animal and Environmental Biology, Physiology, Ecology and Conservation Human Biology, Microbiology, Molecular Biology, Genetics, Cell biology, Genomics and Proteomics, Biochemistry Agriculture, Veterinary Studies, Animal Production, Forest Sciences Biotechnology, Bioinformatics, Pharmaceuticals, Food Technology Theoretical and Applied Mathematics, IT Physics Chemistry and Chemical Engineering Geology, Earth Sciences, Environmental and Atmospheric Sciences, Mining, Geological Engineering, Oceanography, Hydrology Civil and Construction Engineering, Energy, Nuclear and Renewable Energy Engineering Telecommunication Engineering, Electronics, Robotics, Biomedical Engineering, Automation, ICT Industrial Engineering, Mechanics, Metallurgy, Materials, Nanotechnology, Aeronautical, Naval and Aerospace Enginerring Research project / Research Group description (max 2000 characters without spaces) Structural Biology of Protein & Nucleic Acid Complexes and Molecular Machines The group is engaged in the analysis of proteins and nucleic acids complexes using a number of molecular biology and structural biology techniques. Protein-nucleic acid complexes are prepared and crystallized for X-ray diffraction experiments, using synchrotron radiation. The complexes may also be analyzed by cryo-electron microscopy. The final outcome is a detailed 3D view of the molecular structures of interest. This information, together with complementary molecular biology and biochemistry experiments, unveils how these proteins and molecular machines function. Our focus is on the structural analysis of complexes and molecular machines related to DNA transcription, replication and translocation. Our group has also worked on the structural determination of a unique DNA structures, including the first determination of a Holliday junction and the structure DNA three-way junctions. Furthermore, our group has been involved in the characterization of complexes of DNA with different drugs with potential anticancer activity, either intercalators or supramolecular compounds. Job position description (max 2000 characters without spaces) We offer a PhD position to work on the structural analysis of a gene transcription initiaton complex. The complex is formed by the RNA polymerase holoenzyme, the DNA promoter and transcription factors related to pathogenic gene activation. The analysis includes the cloning expression and purification of the proteins involved, and the preparation of the complexes for crystallization and X-ray diffraction and cryo-electron microscopy analysis. The project implies the training on a number of molecular biology, biochemistry and biophysics techniques, as well as computational and computer graphics analysis. Group leader Title (Dr. / Prof./ Mr. / Mrs) Full name: Miquel Coll Capella Email: [email protected] Research Project / Research Group website: https://www.irbbarcelona.org/es/research/structural-biology-of-protein-nucleic-acidcomplexes-and-molecular-machines