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Movie 1: Movie_1_Periodic_cell_movement_during_aggregation.mpg Dictyostelium cells aggregating on a coverslip submerged in phosphate buffer 5hours after the onset of starvation. Cells communicate by propagating cAMP waves which cause the periodic chemotactic cell movement. Time interval between images: 20 seconds Movie 2: Movie_2_Darkfieldwaves.mpg Dictyostelium cells that have been plated in a monolayer on an agar plate show the characteristic macroscopic dark field waves that correspond to the relayed cAMP waves (left panel). The diameter of the Petri dish is 3.5cm. Time interval between images: 20 seconds. The right panel shows the images following image subtraction to enhance the visibility of the dark field waves. Movie 3: Movie_3_Mound_wavepropagation.mpg Higher magnification of a mound with aggregation streams in which cells continue to move towards the aggregations centre (Upper panel). Time interval between images: 10 seconds. The bottom panel shows again the subtracted image to highlight the propagation of the optical density waves that are propagated away along the aggregation streams while the cells move in the opposite direction towards the mound. Movie 4: Movie_4_CRAC_translocation_in_streams.mpg Cells expressing the PIP3 binding PH domain of CRAC fused to GFP which translocates to the leading edge in response to the external cAMP waves. The cells move in an aggregation stream from right to left, while the cAMP waves are propagated in the opposite direction. Detection of the periodic cAMP signal causes the transient translocation of CRAC-GFP to the leading edge and an increase in cell movement speed. This is shown for the cell highlighted in yellow. The graphs show the fluorescence changes at the leading edge of that cell (red curve, measured in a 10x10pixel window at the anterior plasma membrane) indicating the periodic production of PIP3 at this site which also correlates with the changes in cell velocity (white curve). Time interval between images: 10 seconds. Movie 5 Movie_5_Paxillin_sorting.mpg Side view of a mound of wildtype cells containing fluorescently labelled wildtype cells (red) and fluorescently labelled PaxBnull cells (green). As the mound transforms into a slug the dispersed paxBnull cells are restricted to the posterior of the forming slug. Time interval between images: 60 seconds. Movie 6 Movie_6_Cellmovement_in_slugs.mpg Cell movement in the slug is coordinated by periodic cAMP waves that are initiated in the slug tip and propagated backwards along the length of the slug causing the periodic changes in cell velocity. The fluorescence image shows a small number of GFP labelled cells. The three highlighted cells (yellow) were tracked and the corresponding velocities plotted in the graph underneath. Time interval between images: 20 seconds. Movie 7 Movie_7_PaxillinBGFP_in_aggregation_streams.mpg PaxillinB-GFP accumulates at focal adhesion sites that remain stationary once they have been formed. Shown is the ventral surface of cells moving in aggregation streams under TIRF illumination. Time interval between images: 2 seconds. Movie 8 Movie_8_ABDGFP_in_slug.mpg Using the f-actin specific ABD-GFP actin containing containing filaments in the cell cortex are clearly visible in these slug cells (TIRF illumination). Also visible are small spot like putative cell contact sites. Time interval between images: 1 second. Movie 9 Movie_9_MyosinIIGFP_in_slug.mpg MyosinII-GFP marks the cellular localisation of myosin II filaments which are involved in the contraction of the cell posterior through interaction with the actin cytoskeleton. As can be seen MyosinII-GFP filaments are absent from the leading edge of slug cells. Time interval between images: 2 seconds. Movie 10 Movie_10_mesoderm_cell_migration_in_chick_embryo.mpg Upper panel: brightfield image of stage HH4 chick embryo. Middle panel: GFP expressing cells were grafted to the posterior and middle primitive streak and cell movement followed subsequently. The tip of the primitive streak points to the right. Time interval between images: 3minutes. Bottom panel: Cell tracks of the GFP labelled cells over a 6 hour period, the last two hours of migration are shown in green. Movie 11 Movie_11_streak formation Upper left panel brightfield image of developing chick blastodisc, the primitive streak forms diagionally from the bottom and extends towards the top. Upper right panel: sequence showing movement of individual GFP transfected cells, every dot is a single cell. Lower left panel: Vector velocity field calculated from the bright field sequence. Lower right panel: cell tracks calculated from the movement of the GFP transfected cells (upper left panel)