Download Movie 1:

Movie 1:
Movie_1_Periodic_cell_movement_during_aggregation.mpg
Dictyostelium cells aggregating on a coverslip submerged in phosphate buffer 5hours
after the onset of starvation. Cells communicate by propagating cAMP waves which
cause the periodic chemotactic cell movement.
Time interval between images: 20 seconds
Movie 2:
Movie_2_Darkfieldwaves.mpg
Dictyostelium cells that have been plated in a monolayer on an agar plate show the
characteristic macroscopic dark field waves that correspond to the relayed cAMP
waves (left panel). The diameter of the Petri dish is 3.5cm. Time interval between
images: 20 seconds. The right panel shows the images following image subtraction to
enhance the visibility of the dark field waves.
Movie 3:
Movie_3_Mound_wavepropagation.mpg
Higher magnification of a mound with aggregation streams in which cells continue to
move towards the aggregations centre (Upper panel). Time interval between images:
10 seconds. The bottom panel shows again the subtracted image to highlight the
propagation of the optical density waves that are propagated away along the
aggregation streams while the cells move in the opposite direction towards the
mound.
Movie 4:
Movie_4_CRAC_translocation_in_streams.mpg
Cells expressing the PIP3 binding PH domain of CRAC fused to GFP which
translocates to the leading edge in response to the external cAMP waves. The cells
move in an aggregation stream from right to left, while the cAMP waves are
propagated in the opposite direction. Detection of the periodic cAMP signal causes
the transient translocation of CRAC-GFP to the leading edge and an increase in cell
movement speed. This is shown for the cell highlighted in yellow. The graphs show
the fluorescence changes at the leading edge of that cell (red curve, measured in a
10x10pixel window at the anterior plasma membrane) indicating the periodic
production of PIP3 at this site which also correlates with the changes in cell velocity
(white curve). Time interval between images: 10 seconds.
Movie 5
Movie_5_Paxillin_sorting.mpg
Side view of a mound of wildtype cells containing fluorescently labelled wildtype
cells (red) and fluorescently labelled PaxBnull cells (green). As the mound transforms
into a slug the dispersed paxBnull cells are restricted to the posterior of the forming
slug. Time interval between images: 60 seconds.
Movie 6
Movie_6_Cellmovement_in_slugs.mpg
Cell movement in the slug is coordinated by periodic cAMP waves that are initiated in
the slug tip and propagated backwards along the length of the slug causing the
periodic changes in cell velocity. The fluorescence image shows a small number of
GFP labelled cells. The three highlighted cells (yellow) were tracked and the
corresponding velocities plotted in the graph underneath.
Time interval between images: 20 seconds.
Movie 7
Movie_7_PaxillinBGFP_in_aggregation_streams.mpg
PaxillinB-GFP accumulates at focal adhesion sites that remain stationary once they
have been formed. Shown is the ventral surface of cells moving in aggregation
streams under TIRF illumination. Time interval between images: 2 seconds.
Movie 8
Movie_8_ABDGFP_in_slug.mpg
Using the f-actin specific ABD-GFP actin containing containing filaments in the cell
cortex are clearly visible in these slug cells (TIRF illumination). Also visible are small
spot like putative cell contact sites. Time interval between images: 1 second.
Movie 9
Movie_9_MyosinIIGFP_in_slug.mpg
MyosinII-GFP marks the cellular localisation of myosin II filaments which are
involved in the contraction of the cell posterior through interaction with the actin
cytoskeleton. As can be seen MyosinII-GFP filaments are absent from the leading
edge of slug cells. Time interval between images: 2 seconds.
Movie 10
Movie_10_mesoderm_cell_migration_in_chick_embryo.mpg
Upper panel: brightfield image of stage HH4 chick embryo.
Middle panel: GFP expressing cells were grafted to the posterior and middle primitive
streak and cell movement followed subsequently. The tip of the primitive streak
points to the right. Time interval between images: 3minutes.
Bottom panel: Cell tracks of the GFP labelled cells over a 6 hour period, the last two
hours of migration are shown in green.
Movie 11
Movie_11_streak formation
Upper left panel brightfield image of developing chick blastodisc, the primitive streak
forms diagionally from the bottom and extends towards the top. Upper right panel:
sequence showing movement of individual GFP transfected cells, every dot is a single
cell. Lower left panel: Vector velocity field calculated from the bright field sequence.
Lower right panel: cell tracks calculated from the movement of the GFP transfected
cells (upper left panel)