Download Blood and Bone Marrow

Blood and Bone Marrow
Peripheral Blood: Erythrocytes
• RBCs constitute the
largest number of
cells in the blood
• Biconcave discs
• NO NUCLEUS
• Contain Hemoglobin
Peripheral Blood: Platelets
• Derived from
Megakaryocytes in
bone marrow –
formed from small bits
of Megakaryocyte
cytoplasm
• Function in blood
clotting
Peripheral Blood: Leukocytes
• GRANULOCYTES
– Neutrophils
– Basophils
– Eosinophils
• AGRANULOCYTES
– Lymphocytes (T and B cells)
– Monocytes (Macrophages)
Peripheral Blood:
Leukocytes
• GRANULOCYTES
– Neutrophils
– Basophils
– Eosinophils
Granulocytes: Neutrophils
• Most numerous WBC in
blood
• Multilobed nucleus
• Granules:
– Specific granules
– Azurophilic granules
• Function
– 1st wave of cells in acute
inflammation; can
phagocytose bacteria
Granulocytes: Basophils
• Rare!
• Lobulated nucleus often
obscured by granules
• Dark Blue Granules
– Hydrolytic enzymes,
heparin sulfate, histamine,
SRS
• Function
– Role in hypersensitivity and
anaphylaxis
Granulocytes: Eosinophils
• Bilobed nucleus
• Bright pink Granules
– Arginine rich major basic
protein, peroxidase,
histaminase, arylsulfatase
• Function:
– Important in allergic rxns,
parasitic infections, and
phagocytosis of Ab-Ag
complexes
Agranulocytes: Lymphocytes
• About size of RBCs
• Function
– Main functional cells of
adaptive immune
system
– T cells
– B cells
Agranulocytes: Monocytes
• Largest WBCs in
blood smear
• Migrate through blood
to the tissues; once in
tissues they
differentiate into
phagocytes
(macrophages)
Erythrocyte Development
Watch for trends!
Proerythroblast
• Biggest in lineage
• Large central nucleus
with one or two
nucleoli
• Basophilic cytoplasm
b/c ribosomes
• Look for Golgi ghost
Basophilic Erythroblast
• Smaller than
proerythroblast
• Checkerboard
nucleus
(heterochromatic)
• Intense basophilia
(lots of ribosomes!)
Proerythroblast vs Basophilic
Erythroblast
Polychromatophilic Erythroblast
• Smaller than basophilic
erythroblast
• Smaller intensely
heterochromatic nucleus
• Purple/lilac cytoplasm
mix of basophilia from
ribosomes and growing
eosinophilia from
hemoglobin
• LAST MITOTIC STAGE!
Normoblast
• Smaller than
polychromatophilic
erythroblast
• Small, compact,
intensely staining
nucleus that is getting
ready to be extruded
• Eosinophilic
cytoplasm due to
abundant hemoglobin
Erythrocyte
• Smallest
• Eosinophilic due to
hemoglobin
• NO NUCLEUS!