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9765 Clairemont Mesa Blvd, Suite C San Diego, CA 92124 Tel: 858.875.1900 Fax: 858.622.0609 TM173 Antibody (C-term) Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP9747b Specification TM173 Antibody (C-term) - Product Information Application Primary Accession Reactivity Host Clonality Isotype Clone Names Calculated MW Antigen Region WB, FC,E Q86WV6 Human Rabbit Polyclonal Rabbit Ig RB24462 42193 311-340 TM173 Antibody (C-term) - Additional Information Gene ID 340061 Other Names Stimulator of interferon genes protein, hSTING, Endoplasmic reticulum interferon stimulator, ERIS, Mediator of IRF3 activation, hMITA, Transmembrane protein 173, TMEM173, ERIS, MITA, STING Target/Specificity This TM173 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-340 amino acids from the C-terminal region of human TM173. Western blot analysis of lysate from U-937 cell line, using TM173 Antibody (C-term)(Cat. #AP9747b). AP9747b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Dilution WB~~1:1000 FC~~1:10~50 Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. Precautions TM173 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. TM173 Antibody (C-term) (Cat. #AP9747b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. TM173 Antibody (C-term) - Background TM173 Antibody (C-term) - Protein Information Name TMEM173 Synonyms ERIS, MITA, STING Function Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG doublestranded DNA from viruses and bacteria delivered to the cytoplasm. Acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol: upon binding of c-di-GMP or cGAMP, autoinhibition is alleviated and TMEM173/STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Essential for the induction of IFN-beta in response to human herpes simplex virus 1 (HHV-1) infection. Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasm, perinuclear region. Cytoplasm Note=In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited Tissue Location Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages. TM173 Antibody (C-term) - Protocols Provided below are standard protocols that you may find useful for product applications. • • • • • • • • Powered by TCPDF (www.tcpdf.org) Western Blot Blocking Peptides Dot Blot Immunohistochemistry Immunofluorescence Immunoprecipitation Flow Cytomety Cell Culture Acts as a facilitator of innate immune signaling. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. TM173 Antibody (C-term) - References Sun, W., et al. Proc. Natl. Acad. Sci. U.S.A. 106(21):8653-8658(2009) Zhong, B., et al. Immunity 30(3):397-407(2009) Graubert, T.A., et al. PLoS ONE 4 (2), E4583 (2009)